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What is viral transport medium ?

Posted on 2022年5月6日2022年5月6日 by Dr. Lee

Viral transport medium is a protective liquid medium added to the virus sampling tube to protect the samples after nasopharyngeal swab sampling and is usually called virus preservation solution in China.

Usually, in nucleic acid testing (PCR swab test), we cannot perform nucleic acid PCR directly at the sample collection site.

We need to add virus preservation solution (VTM) to the samples collected by swab for transfer check.

Viral Transport Media Kits Manufacturer

What is a virus?

Viruses are microorganisms whose structure is very simple.

There is no way to complete the process of self-replication.

They need the help of host cells and the nutrition of the host to complete virus replication.

The virus can infect humans as well as many other animals.

Influenza viruses such as common infectious diseases are usually RNA viruses.

There are many common viruses that cause human infection, which can cause respiratory diseases, such as cold virus, influenza virus, HIV and influenza A virus.

Since it has no cellular structure of its own, the virus itself cannot replicate.

But invades the gene into the host cell and replicates the new virus with the help of the latter’s replication system.

In conclusion, viruses are diverse and can cause damage to multiple parts of the body. “

After the virus sample is collected, in order to maintain the activity of the virus sample and prolong the survival time of the virus in the sample, the sampling swab ( flocking swab ) will be put into the preservation solution ( viral transport media ) for preservation and transportation.

What is the viral transport medium used for?

PCR test

The inactivated viral transport medium can perform protein denaturation on fresh clinical virus specimens to inactivate the virus. Prevent secondary infection and ensure the safety of transportation and testing personnel. The inactivated samples can be used with a variety of virus extraction kits.

The nucleic acid extraction instrument can quickly extract nucleic acids.

At the same time, with the respiratory pathogen PCR detection kit (VTM Kits) to achieve rapid detection. The specificity and sensitivity are not affected.

Virus preservation solution has many functions:

1. The operation is simple, and the liquid contains ingredients that can denature proteins, thereby inactivating the virus;

2. Contains inhibitors to protect the stability of viral nucleic acid to a greater extent and greatly improve the nucleic acid extraction rate;

3. Stored at room temperature for 12 months before sampling.

The airtightness is good, which is convenient for storage and transportation and saves transportation costs.

Cell medical (Dongguan) Co., Ltd.

Viral transport media include inactivated and non-inactivated viral transport media.

Contains Hank‘s Liquid, Potassium Chloride, Magnesium Chloride, Calcium Chloride, BSA, etc. The combination of various antibiotics has antibacterial and antifungal effects. Bovine serum albumin (BSA), as a protein stabilizer, can form a protective film on the protein shell of the virus. Making it difficult to decompose and ensuring the integrity of the virus; Hank’s buffer The neutral environment constructed by the agent helps to increase the survival time and infection stability of the virus.

What is viral transport medium?

Nasopharyngeal Swab

Inactivated and non-inactivated virus preservation solutions: usually used for the collection and transportation of clinical influenza, avian influenza, hand, foot and mouth virus, measles and other virus specimens, as well as specimens such as mycoplasma, ureaplasma, chlamydia, etc.

1. For monitoring and sampling of infectious pathogenic microorganisms by disease control departments and clinical departments.

It is suitable for a sampling influenza virus (common influenza, highly pathogenic avian influenza, H1N1 influenza virus, etc.), hand, foot and mouth virus and other types of viruses.

2. To transport nasopharyngeal flocked swab specimens or tissue specimens from specific parts from the sampling site to the testing laboratory for PCR extraction and detection.

3. Preserve nasopharyngeal swab samples or tissue samples from specific parts for necessary cell culture.

Pooled Sample Test Viral Transport Media Kits Manufacturer

Posted on 2022年5月5日2022年5月5日 by Dr. Lee

The epidemic is an order, prevention and control are a responsibility, and life is more important than Mount Tai.

One-time use of Viral Transport Medium accelerates production, Cell medical (Dongguan) Co., Ltd. has a daily production capacity of more than 1 million sets of Viral Transport Media kits.

One-time use Virus Sampling Tube, Accelerated Overtime Production, 10ml Viral Transport Media Tube, 20ml Viral Transport Media Tube, 30ml Viral Transport Media Tube.

20-in-1: Pooled Sample Test, originally originated from a single set of samples to 5-in-1, 10-in-1, and maybe 30-in-1, 40-in-1, and 50-in-1 in the future.

The purpose of collecting and mixing is to improve efficiency and quickly screen positive patient samples.

How strong is the detection ability of 10 people’s sample nucleic acid (PCR test) mixed together?

The new wave of the epidemic has affected the hearts of people all over the world. Many people are cheering on the Internet to overcome the epidemic. However, in the recent period, the outbreak of asymptomatic patients and local confirmed patients has not yet reached an inflection point. cases increased.

Why do 10-in-1 Pooled Sample Test?

Mainly because of the following three advantages.

1. The speed of nucleic acid detection can be increased by 10 times.

Citizens line up in an orderly line for PCR swab testing

In the past nucleic acid testing, each person took a test tube.

After the test, we put the sample into the test tube for preservation.

The samples of 10 people: Stored in a test tube.

If tens of thousands of people in the city do nucleic acid swabs alone.

Then, we need millions and tens of millions of collection tubes and a large number of medical personnel.

In this way, the speed of nucleic acid detection will be very slow. Unable to achieve the desired effect.

2. It can quickly identify the positive infected people in the crowd.

Now the infected people are all asymptomatic patients with fever and no cold. It is impossible to detect them by ordinary methods. Only through nucleic acid testing can the hidden dangers in the crowd be excluded.

If everyone is tested individually, the speed of nucleic acid testing will be particularly slow. Over time, people queuing for nucleic acid testing are likely to cause cross-infection.

Completing the test as soon as possible and excluding infected people from the crowd as soon as possible can also reduce the scope of infection, and the mixed detection method for each person can improve the speed and reduce the scope of infection.

If there is one positive in it, wouldn’t it cause cross-infection?

Even if the other nine were negative, they would definitely be implicated.

3. Can reduce the cost of nucleic acid testing.

Although in some places our nucleic acid tests are all free, and people do not need to pay a penny, that is because all these nucleic acid costs are paid for by our government.

Our government will uniformly purchase test reagents for nucleic acid detection, and then uniformly pay for some of the testing costs.

There are millions of people in a city, and the cost of these nucleic acid tests is a lot of money.

So what is the principle behind this 10-person sample being mixed together?

Nucleic acid detection using this mode is to deal with large-scale nucleic acid detection.

Each group of 10 people, after completing the test, put the flocked swab samples into a collection tube, and then centrally send them to the local laboratory for testing.

Medical Swab Test Kit specimen collection sterile nylon flocked swab

If these 10 people are all negative, then it proves that these 10 people are all safe and do not need separate isolation and separate testing.

If one of the 10 people is positive, the remaining 9 people will have special epidemic prevention personnel.

These 9 people will be sent to the isolation area for individual isolation, and then each person will be tested individually every day.

After 14 days of isolation, in In the past 14 days, the results of these 9 people are all negative, which can ensure safety and allow them to return to normal life.

Nucleic acid detection using this mode is to deal with large-scale nucleic acid detection.

Each group of 10 people, after completing the test, put the flocked swab samples into a collection tube, and then centrally send them to the local laboratory for testing.

Pooled Sample Test Viral Transport Media Kits Manufacturer

Viral Transport Media Kits Manufacturer

Cell medical (Dongguan) Co., Ltd.

Product name: Disposable virus sampling tube ( Viral Transport Media)

Model: CM-F005-30 (12ml)

Specification: 12mL/tube

Type: inactivated/non-extinguishing/sample release agent

Scope of application: nucleic acid sampling, for new coronavirus sampling, COVID-19, coronavirus sampling, nucleic acid sampling, collection, transportation and storage of virus samples, etc.

Contraindications: none

20 in 1 virus sampling tube Manufacturer: Cell medical (Dongguan) Co., Ltd.

Output: 200,000 sets per day

10 mixed 1 production capacity of 1 million sets per day

Throat swab production capacity: 10 million per day.

Flocked Swab Influenza | Comprehensive Flu Swab Test Guide

Posted on 2022年5月4日2022年5月4日 by Dr. Lee

Flu Flocked Swab Influenza

Influenza refers to the common cold, which is an acute respiratory infectious disease caused by the influenza virus.

It is highly contagious and mainly spreads through droplets, such as coughing and sneezing. Influenza generally occurs in winter and spring.

The current influenza detection method is the rapid influenza antigen detection method, which detects “flux A” and “flu B” antigens.

Laboratory staff collect samples through throat swabs.

In fact, in inspection work, it is important to know the quality of the specimen in order to obtain accurate and reliable results.

Throat swab specimens: Professionals can avoid many false-negative influenza tests caused by improper specimen sampling.

The precautions for flu throat swabs are basically the same as those for nucleic acid detection throat swabs. Because the same part is sampled.

There are two types of flu throat swabs: nasopharyngeal swabs and oropharyngeal swabs.

What precautions should I take when taking a throat swab test for flu?

1. Oropharyngeal swab The collection method of the oropharyngeal swab is to let the subject open his mouth, then use a long pharyngeal swab to wipe the patient’s pharynx, bilateral frontal arches and tonsils, and take out the local secretions. Into the catheter of the throat swab and sent to the laboratory for testing.

2. Nasopharyngeal swabs The collection method of nasopharyngeal swabs is to use sterilized throat swabs to penetrate deep into the nasal cavity of the subject and reach the nasopharynx, and extract secretions and nasopharynx by rotating or wiping.

Epithelial cells put a section with secretions and epithelial cells into a sterile container and then carry out pathogenic detection.

Flu swab material

Swab options for flu testing include flocked swabs, cotton-tipped swabs, rayon tipped swabs, and foam-tipped swabs.

Cotton tip swab:

The original swab tips were all cotton, mainly based on their cleaning power and water absorption. Cotton swabs are very inexpensive and readily available, and are widely used in medical wound management after sterilization with ethylene oxide or autoclaving, as well as in forensic crime scene forensics. In the absence of alternatives, cotton swabs can be used in almost a variety of scenarios.

However, cotton-tipped swabs also have some problems. Due to the low release rate of cotton, natural material for samples, and some of the natural fatty acids in it can damage microorganisms, they are less and less used for sample collection.

Rayon head swab:

Rayon—a synthetic fiber made from wood pulp. Although classified as synthetic fiber, it is essentially a natural material. Compared to cotton, rayon is produced during the process of removing substances that may damage the sample or react with the sample during transportation and handling , so it is generally used for sampling for diagnostic testing.

Polyester Fiber Tip Swabs:

The head material of the polyester fiber swab is made of polyester long fiber winding, which was first introduced into the field of medical testing by DuPont. Polyester fibres are proven for microbial collection, rapid diagnostics, and PCR testing.

Polyester swabs have a high release rate but are more expensive than cotton and rayon swabs.

Foam tip swab:

China Medical Nasal Foam Swab Factory And Manufacturers

Swabs made from medical-grade polyurethane are an excellent material for diagnostics, especially cell sampling. The swab tip of this swab is usually manufactured as a sheet with a surface hole count of 100ppi (holes per inch).

This material has good absorption of hydrophilic substances and poor absorption of hydrophobic substances. Used in some scenarios that require resistance to chemical corrosion. Also used for DNA and microbial sampling.

Flocked Swabs:

Flocking technology was first developed for cosmetic and industrial production. (Learn more)

After the flocking process was introduced into swab production. It replaced the traditional swab head, and the swab head was brought to a new level.

vtm swab

Due to the flocking process and the multi-branched polyester fibre material used, the flocked swabs have a very high absorption and release rate and are proven to be equally efficient for molecular sampling and absorption. Designated by some institutions for nucleic acid sampling as well as some research areas for genomic analysis.

The evolution of swabs is not limited to changes in materials. New demands in the fields of nursing, testing, and scientific research make swabs continue to evolve in multiple dimensions. The swab also not only acts as an applicator, but also acts as some transport medium.

Precautions include the following:

① Do not drink water before the throat swab test, at least 30 minutes in advance, because drinking water before the test will dilute the virus attached to the pharynx, especially drinking hot water will inhibit the activity of the virus.

In order to improve the accuracy of throat swab nucleic acid detection, do not drink water 15 to 30 minutes before the test to ensure that the throat swab has high accuracy.

②. For patients with good throat exposure, it is not recommended to take too long “ah~” sounds to reduce the risk of occupational exposure to the sampler.

③ To prevent vomiting, do not eat within two hours before the throat swab test.

④. Do not smoke, drink, or chew gum within half an hour before the throat swab test.

During the throat flocked swab sampling, the patient may have clinical symptoms such as nausea or nasopharyngeal discomfort, but the symptoms can subside quickly, so the patient should not be nervous and cooperate actively during the examination. At the same time, the subjects should not smoke, drink, or chew gum within half an hour before the sample collection.

If it is because of the throat swab the child, the parents must soothe the child’s emotions, communicate with the child patiently, and try not to move the child’s head as much as possible.