Viral Transport Kits | Viral Transport Medium | VTM Kit

Winter is coming, the cold current is coming, the goose feather like snow is flying all over the sky, and the whole world is wearing a white coat.

Many customers once again throw this “soul question” to us. “Can your Viral Transport Kits adapt to the cold environment?”

He answer is yes. Let’s follow Cell medical Dr. Li for experimental verification!

Experimental purpose

Verify that the Viral Transport Medium of cell medical (Dongguan) Co., Ltd. has no significant impact on its service performance after low-temperature freezing and thawing.

Experimental scheme

Simulate the process of low-temperature freezing during transportation, place the sampling tube in a low-temperature environment for 24 hours, and then melt at room temperature. The sampling tube products stored normally without freezing and thawing were used as the control. Each group was added with virus mixture, stored at 4 ℃ for 24 hours, and then bathed in water at 56 ℃ for 30 minutes for nucleic acid extraction and nucleic acid detection. Set 2 repetitions for each group.

1. Low temperature icing simulation: set two low temperature gradients and place the sampling tube in two low temperature environments of – 20 ℃ and – 80 ℃ respectively.

2. Mixing of virus solution: We used two different concentrations of virus solutions, representing high and low concentration gradients respectively. The copy numbers of virus solution were 106 copies / ml and 104 copies / ml, respectively. Simulate the actual sampling process, add the virus solution in the volume ratio of 1:10 to the sampling solution, and the virus solution is 100 μ 50. 1ml of sampling solution and add 100 ml to it μ L of oral liquid.

3. After mixing each group, place it in 4 ℃ environment for 24 hours and inactivate it in 56 ℃ water bath for 30 minutes.

4. Extract the nucleic acid of each group of virus, and detect the virus content of each group by RT qPCR. The CT value is used as the basis for result evaluation. The higher the CT value, the higher the detected concentration.

5. The experimental groups are as follows:

Main experimental materials

Sampling solution: virus preservation solution produced by cell medical (Dongguan) Co., Ltd.
virus diluent: the virus isolation serum-free medium of Cell medical was used for gradient dilution.
virus for verification: porcine epidemic diarrhea virus. Its structure and genome size are similar to those of New Coronavirus.
nucleic acid extraction reagent: non cleavage nucleic acid extraction reagent (magnetic bead method).

Viral Transport Kits Experimental results.

1. Product appearance.

the color is consistent without precipitation.

2. Performance evaluation results

amplification curve.

A. amplification curve of each experimental group and negative control group:

The groups with earlier peaks are the amplification curves of high concentration groups, and the groups with later peaks are the amplification curves of low concentration groups.

Empirical conclusion.

The Viral Transport Medium products of Cell medical (Dongguan) Co. ,Ltd. will not affect its service performance after one-time freezing and thawing.

And can fully meet the requirements of low-temperature transportation and low-temperature preservation of virus samples.