What kind of virus sampling tube do you know? Does the following look like what you know? The following is introduced to you by Disposable Virus Sampling Tube Manufacturers — Cell medical (Dongguan) Co., Ltd.
What is Virus Sampling Tube? When should the virus preservation solution be used?
Disposable Virus Sampling Tube Kits
Virus transport medium, virus preservation solution: a protective liquid medium added to the virus sampling tube to protect the samples after nasopharyngeal swab sampling.
Generally, in the process of nucleic acid detection, we cannot directly conduct nucleic acid PCR experiments at the sample collection site.
If we need to check the samples collected by the flocked swab, we need to add the virus preservation solution.
Why use a virus sampling tube?
First of all, we have to understand what is a virus? Why does it need a virus sampling tube?
virus sampling tube
What is a virus?
Virus: a non-cellular form composed of nucleic acid molecules and proteins.
It lives on parasitic hosts and is an organic species between living and non-living bodies.
It is one end of DNA or RNA wrapped in a protective shell.
Through the mechanism of infection, these simple organisms can use the host’s cellular system to replicate themselves but are unable to grow and replicate independently.
Viruses can infect almost all living organisms with cellular structures.
Virus detection is different from conventional biochemical detection.
The virus itself is a simple microorganism and must be parasitic in living cells.
After sampling, when the virus leaves the host cell, its protein shell and nucleic acid degrade rapidly in the sampling tube.
So that the nucleic acid During the test, it is impossible to determine whether the initially collected sample contains the virus, which is likely to cause false negatives.
A virus delivery medium (virus sampling tube), a general-purpose sampling product: for sampling various virus samples, chlamydia samples, and ureaplasma samples.
Short-term storage and transportation at 2-8°C, and -80°C refrigerator or liquid nitrogen Long-term preservation in the environment.
1. For monitoring and sampling infectious pathogenic microorganisms by disease control departments and clinical departments.
Suitable for influenza virus (common influenza, highly pathogenic avian influenza, influenza A H1N1 virus, etc.), hand, foot and mouth virus and other types of virus sampling. It is also used for sampling Mycoplasma, Chlamydia, Ureaplasma, etc.
2. To transport nasopharyngeal swab specimens or tissue specimens from specific parts from the sampling site to the testing laboratory for extraction and testing.
3. Preserve nasopharyngeal swab samples or tissue samples from specific parts for necessary cell culture.
4. For short-term storage and transportation of virus samples at 2-8°C and long-term storage in a -80°C refrigerators or liquid nitrogen environments.
There are two types of virus preservation solutions: inactivated and non-inactivated
The inactivated virus preservation solution is mainly a virus lysis type preservation solution improved by nucleic acid extraction lysis solution, which is added with a high concentration of lysis salt, which can quickly and efficiently deactivate the virus protein in the sample to be tested and can effectively prevent infection.
At the same time, it also contains an RNase enzyme inhibitor, which can protect virus nucleic acid from degradation.
As long as the subsequent NT-PCR experiment can detect the nucleic acid of the virus, we can diagnose it.
Stored at room temperature for a relatively long time.
The non-inactivated preservation solution is mainly a virus maintenance solution that is improved on the basis of the transport medium.
It can retain the protein coat of the virus and the viral nucleic acid DNA or RNA at the same time so that the virus has the integrity of the protein epitope and nucleic acid in vitro.
Of course, there is also a certain risk of infectivity when the operation is wrong.
It is necessary to keep a strictly low temperature for long-term storage after sampling.
No matter what kind of virus preservation solution it is, we must test it as soon as possible after sampling or keep it strictly at a low temperature to ensure that the detection is accurate.
Due to the rapid reproduction and mutation of the virus, the virus preservation solution plays a crucial role in maintaining the stability of the virus sample.
Manufacturers CellMedical Virus Sampling Tube Kits Advantages
1. Add protein-stabilizing components such as BSA to the sampling solution to reduce the speed of virus decomposition and greatly improve the positive rate of virus isolation.
2. It is stable at room temperature and can keep its activity within 1 year.
3. Gentamicin replaces penicillin to avoid allergic reactions;
4. Using flocked swabs, the sample has a higher collection rate and release rate;
Samples can be stored for a long time (-20 ℃ ordinary refrigerator, -80 ℃ low-temperature refrigerator, -197 ℃ liquid nitrogen);
6. Thickening and anti-leakage design to ensure that the sample does not leak, in line with WHO regulations and biosafety regulations.
