What Is Virus Transport Medium

What Is Virus Transport Medium

The virus transport medium is novel and unfamiliar to many small partners, but it is of great significance for the detection of various viruses.

The new crown virus has tortured human beings enough.

In the face of a large number of COVID-19 patients, some places are still rising.

Therefore, as soon as there are new additions, many places will focus on the new crown nucleic acid detection.

The virus preservation solution plays a very important role.

Virus Transport Medium with Specimen collection swab

Virus Transport Medium with Specimen collection swab

At present, the nucleic acid detection of the new coronavirus is mainly the detection of viral nucleic acid.

Virus transmission medium is a cell preservation liquid, which protects the virus to be detected by immersing the flocked swab head with the virus sample into the transport tube.

It is mainly suitable for the new coronavirus, Collection, preservation and transportation of common virus samples such as the influenza virus.

To collect throat swabs, nasal swab or tissue samples from specific parts.

The stored samples: for subsequent nucleic acid detection or clinical experiments such as virus culture.

Since the virus is a parasitic organism, it cannot survive outside the body after sampling.

If it cannot be detected in time, it needs to be placed in a virus preservation solution for preservation. (Virus Transport Medium)

In order to protect the safety of the virus detection environment and the safety of the detection personnel, inactivated virus preservation solution is actually a better choice!

FLOCKED SWAB

FLOCKED SWAB

For different detection purposes, we need to use different virus preservation solutions for preservation.

The two widely used preservation solutions have their own characteristics.

In order to meet different detection requirements and different experimental conditions for virus detection, it is necessary to use different preservation solutions.

The inactivated virus preservation solution can inactivate the virus.

How to detect it after inactivation?

The purpose of our use of inactivated preservation solution is to lyse nucleic acids, release nucleic acids and then carry out nucleic acid detection by subsequent real-time fluorescent RT-PCR.

Doctors use inactivated virus transport media to determine whether the sample contains virus characteristic nucleic acid, that is, whether we carry the virus.

Inactivated virus transport medium:

A virus lysis type preservation solution improved by nucleic acid extraction lysis solution.

Effectively prevent the secondary infection of the operator, but it also contains inhibitors, which can protect the viral nucleic acid from being degraded.

This enables subsequent detection by NT-PCR.

Moreover, stored at room temperature for a relatively long time, which saves the cost of virus sample storage and transportation.

Non-inactivated virus transport medium:

A virus maintenance liquid type preservation solution improved on the basis of the transport medium.

It maintains the activity of the virus in vitro and the integrity of antigens and nucleic acids.

The protective viral protein shell makes it difficult to decompose and maintains the originality of the virus sample to a great extent.

For nucleic acid extraction and detection, the experimenters also use the transport medium for virus culture and isolation and maintain the activity of virus samples.

In addition to nucleic acid extraction and detection, the inspector also uses this preservation solution for virus culture, isolation and antigen detection.

What is Virus Sampling Tube

What is Virus Sampling Tube

Since the outbreak of COVID-19 pneumonia, through multiple rounds of new coronavirus nucleic acid tests on personnel in relevant areas.

A virus sampling tube is a set of centrifuge tubes for sampling and transportation of influenza virus (including COVID-19), hand, foot and mouth virus and other viruses in the microbial sampling transportation tube.

Also known as the specimen transport tube, the virus transport medium has now become a commercial product.

Disposable sampling tube kit

The disposable sampling tube consists of a swab + sampling tube + virus preservation solution.

That is flocked swab, plastic virus sampling tube and virus transport medium.VTM

We have been able to quickly and effectively identify infected persons and treat them in a timely manner.

At the same time, we can quickly divide risk management areas and effectively Prevent the further spread of the epidemic.

The high efficiency and accuracy of the nucleic acid Swab Test is the “gold standard” for the diagnosis of new coronary pneumonia.

VTM KIST Virus Sampling Tube

Disposable virus sampler

Disposable sampling tube manufacturer Cell medical

In order to support the epidemic prevention work, Cell medical (Dongguan) Co., Ltd. has launched a single-use sampling tube.

Which meets the requirements of the COVID-19 virus nucleic acid sampling and testing technical specifications issued by the country.

VTM kits are suitable for the collection, transportation and storage of throat swab or nasal swab samples.

At the same time, our products have a variety of models and specifications, which can be used with multi-planting wool swabs, which are suitable for use by medical units at all levels.

At the same time, the kit contains a biosafety bag for the preservation and transfer of specimens after collection to prevent Accidental leakage, etc., improving the safety of use.

 

Flocked swab+tube with Viral transport medium

How to Choose a Correct Virus transport medium for COVID-19 Testing

How to Choose a Correct Virus transport medium for COVID-19 Testing

Disposable virus sampling tube

Disposable virus sampling tube

At present, the form of New Coronavirus is still very serious.

As the first step of covid-19 detection, the importance of sample collection and preservation is beyond doubt.

The influencing factor is sample collection; If there is a problem with the collected sample, the result is invalid regardless of the subsequent treatment.

Selecting appropriate virus transport media and collecting flocking swabs can improve the efficiency of new crown detection.

Almost all virus preservation solutions on the market contain non inactivated viruses directly, resulting in high infection risk for sampling, transportation and testing personnel.

Therefore, the front line of epidemic prevention needs to be able to inactivate virus samples directly.

At 37 ° C, we compared the preservation effects of cell medical (Dongguan) preservation solution containing guanidine hydrochloride and guanidine isothiocyanate with that without guanidine salt on avian coronavirus.

After 1-7 days of storage, under the same condition of 37 ℃, the preservation rate of virus nucleic acid was basically maintained at 100% when it was preserved with cell medical (Dongguan) preservation solution;

·Use virus preservation solution that can protect the sample from high temperature inactivation.

The results showed that high temperature inactivation had no significant effect on the integrity of coronavirus nucleic acid when coronavirus nucleic acid samples were preserved with high temperature inactivator.

The virus preservation solution developed by Cell medical (Dongguan) company is divided into inactivated and non inactivated. Different preservation solutions can be selected according to different test requirements and test conditions. If you have any questions or needs, please call us. Choosing cell medical (Dongguan) virus transport media solution in China is your right choice.

Furan compounds (guanidine isothiocyanate or guanidine hydrochloride, etc.) are typical protein denaturants.

They are often used to extract cell lysis in nucleic acid and inactivate virus. However, the furamethoxam component does not have the ability to work at room temperature.

Because the ability to preserve viral nucleic acid is easy to lead to sample degradation, it is not suitable for preserving new coronavirus samples. Therefore, it is strongly recommended that you choose the preservation solution without guanidine salt.

To this end, we call on the majority of medical personnel to select non inactivated virus transport media if they take samples for the purpose of nucleic acid detection and do not need to culture live viruses.

Vtm Swab | Virus Transport Medium with Sampling Swab

Vtm Swab | Virus Transport Medium with Sampling Swab

Use a virus medium that inactivates the virus.

At present, covid-19 detection is mainly to detect viral RNA, which needs to divide the virus.

Therefore, as long as the integrity and stability of virus nucleic acid can be guaranteed, there is no need to preserve live virus.

Therefore, in the current situation of fighting COVID-19, it is ideal to inactivate the virus while collecting samples. Some manufacturers have also introduced inactivated transport medium, but its inactivating component is guanidine salt.

The results showed that the salt forming component storage solution had insufficient storage capacity for viral nucleic acid at room temperature, which was easy to cause sample degradation.

Therefore, we recommend the use of virus preservation solution containing non guanidine protein denaturants.

The volume of solution in the sampling tube should be appropriate.

① When sampling each sample separately, we recommend that you select a virus sampling tube containing 1ml storage solution to ensure the correct storage capacity. It can not only meet the downstream needs of nucleic acid extraction and detection. Compare with 3ml storage solution. Triple the virus concentration!

② For large-scale preliminary screening and mixing test, we should choose to put the samples of 3-5 people into the sampling tube.

COVID-19 Pooled Sample Testing.

Nasal swab virus sampling kits Virus transport medium for COVID-19

Nasal swab virus sampling kits Virus transport medium for COVID-19

Please contact us soon

·Use a virus preservation solution that can transport and preserve samples at room temperature.

If the delivery temperature does not meet the standard, the viral nucleic acid may degrade, resulting in a “false negative” test result!

On the contrary, if cell medical uses a virus storage solution that can transport and stably store virus nucleic acid at room temperature.

We solved the problem of sample transportation and improved the accuracy of test results.

·Use virus preservation solution that can protect the sample from high temperature inactivation.

However, the research reports of Beijing Center for Disease Control and prevention in the Journal of “clinical chemistry” and Beijing Preventive Medicine Research Center show that high temperature inactivation can lead to the degradation of virus nucleic acid, thus reducing the detection rate of virus nucleic acid. Therefore, The false negative rate of New Coronavirus nucleic acid detection is high.

The Virus transport medium for COVID-19 developed by cell medical (Dongguan) Co., Ltd. include: inactivated and non inactivated.

If you have any questions or needs, please call us.

Choosing cell medical virus transport media solution in China is your right choice.

Please contact us soon

Viral Transport Kits | Viral Transport Medium | VTM Kit

Viral Transport Kits | Viral Transport Medium | VTM Kit

Viral Transport Kits | Viral Transport Medium | VTM Kit

Winter is coming, the cold current is coming, the goose feather like snow is flying all over the sky, and the whole world is wearing a white coat.

Many customers once again throw this “soul question” to us. “Can your Viral Transport Kits adapt to the cold environment?”

He answer is yes. Let’s follow Cell medical Dr. Li for experimental verification!

Experimental purpose

Verify that the Viral Transport Medium of cell medical (Dongguan) Co., Ltd. has no significant impact on its service performance after low-temperature freezing and thawing.

Experimental scheme

Simulate the process of low-temperature freezing during transportation, place the sampling tube in a low-temperature environment for 24 hours, and then melt at room temperature. The sampling tube products stored normally without freezing and thawing were used as the control. Each group was added with virus mixture, stored at 4 ℃ for 24 hours, and then bathed in water at 56 ℃ for 30 minutes for nucleic acid extraction and nucleic acid detection. Set 2 repetitions for each group.

1. Low temperature icing simulation: set two low temperature gradients and place the sampling tube in two low temperature environments of – 20 ℃ and – 80 ℃ respectively.

2. Mixing of virus solution: We used two different concentrations of virus solutions, representing high and low concentration gradients respectively. The copy numbers of virus solution were 106 copies / ml and 104 copies / ml, respectively. Simulate the actual sampling process, add the virus solution in the volume ratio of 1:10 to the sampling solution, and the virus solution is 100 μ 50. 1ml of sampling solution and add 100 ml to it μ L of oral liquid.

3. After mixing each group, place it in 4 ℃ environment for 24 hours and inactivate it in 56 ℃ water bath for 30 minutes.

4. Extract the nucleic acid of each group of virus, and detect the virus content of each group by RT qPCR. The CT value is used as the basis for result evaluation. The higher the CT value, the higher the detected concentration.

5. The experimental groups are as follows:

Main experimental materials

  •   Sampling solution: virus preservation solution produced by cell medical (Dongguan) Co., Ltd.
  •  virus diluent: the virus isolation serum-free medium of Cell medical was used for gradient dilution.
  •   virus for verification: porcine epidemic diarrhea virus. Its structure and genome size are similar to those of New Coronavirus.
  •   nucleic acid extraction reagent: non cleavage nucleic acid extraction reagent (magnetic bead method).

Viral Transport Kits Experimental results.

1. Product appearance.

the color is consistent without precipitation.

2. Performance evaluation results

amplification curve.

A. amplification curve of each experimental group and negative control group:

The groups with earlier peaks are the amplification curves of high concentration groups, and the groups with later peaks are the amplification curves of low concentration groups.

Empirical conclusion.

The Viral Transport Medium products of Cell medical (Dongguan) Co. ,Ltd. will not affect its service performance after one-time freezing and thawing.

And can fully meet the requirements of low-temperature transportation and low-temperature preservation of virus samples.

Viral Transport Kits:composed of flocked swabs and test tubes with Viral Transport Medium(VTM)

 

All about the Nasal Swab

All about the Nasal Swab

All about the Nasal Swab

Nasal Swab:nasopharyngeal np swab.

The specimens collected from nucleic acid samples are mainly pharyngeal swabs, which are divided into “nasopharyngeal swabs” and “oropharyngeal swabs“.

Nasopharynx swab and oropharyngeal swab only have different sampling paths. Oropharyngeal swab is to collect oropharyngeal specimens by entering the sampling swab through the mouth, and nasopharyngeal swab is to collect nasopharyngeal specimens through the nose.

How to do a nasopharyngeal swab?

A man was doing a nasopharyngeal swab sample

A man was doing a nasopharyngeal swab sample

Nasopharyngeal swab sampling method.

Hold a swab close to the nostril to enter, and slowly go deep along the bottom of the lower nasal tract.

Since the nasal tract is curved, do not use too much force to avoid trauma and bleeding.

When the top of the swab reaches the posterior wall of the nasopharyngeal cavity, rotate it gently for one week (stay for a moment in case of reflex cough), then slowly take out the swab and immerse the swab head into a tube containing 2 ~ 3ml virus preservation solution.

About Nasal swab sample collection.

How to take a swab sample?

Ask the patient to take off the mask and blow his nose with a paper towel to remove excess secretions from the nasal cavity. Remove the swab from the package.

Tilt the patient’s head back slightly to make the nasal passage easier to pass through. Ask the patient to close their eyes to reduce the mild discomfort of the operation.

Gently insert the swab over the bottom of the nasal canal along the nasal septum until the nasopharynx stops when resistance is felt.

Insert the cotton swab parallel to the upper jaw into the nostril.

If you feel resistance when passing, step back and try to reinsert it at another angle to bring it closer to the bottom of the nasal canal.

The depth of the swab should be equal to the distance from the nostril to the opening outside the ear.

CDC recommends placing the swab for a few seconds to absorb enough secretions, and then slowly removing it while rotating the swab.

Some organizations may also recommend rotating the cotton swab several times before taking it out.

After removal, ask the patient to put on the mask again.

Specimen processing

Open the virus transport medium and insert the swab into the collection tube.

Break the swab at the groove and discard the remaining part. Seal the labeled collection tube and place it in the biohazard garbage bag.

According to convention, you can return the sample to its original packaging for disposal.

Follow CDC instructions for direct treatment of swab specimens or place swabs in medium with or without refrigeration.

Nasal swab test swab material.

Nylon flocked swab—the most widely used sampling swab material at present

Cell medical flocked swabs,floqswabs.

Flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.

CM’s production of millions of sampling swabs helps epidemic control.

Our flocked swabs are exported to England,America.Canada,Russia,Egypt,Greece,France,Germany,Japan Japan,Australia,Brazil,China nasal swabing. Argentina,Columbia,Iraq,Denmark,Philippines,Norway,Switzerland,Cambodia,Burma.Portugal. Pakistan.Bangladesh.romania.Malaysia flocked swab.Maldives.Afghanistan.Iceland.Ireland.

Nasopharyngeal swab near me

flocked swab application

flocked swab application

 

 

 

Virus Transport Medium

What is the Virus Transport Medium and What is Its Role?

What is the Virus Transport Medium and What is Its Role?

a medium used for the collection, transport and preservation of pathogenic bacteria or microbial samples. Including liquid and solid.

Generally,for the transportation and preservation of clinical samples.

It is used by medical units for pre-treatment of pathogenicity test to obtain pathogenic bacteria samples.

  Inspection principle

Different formulations adopted by different types of transport media can improve and enhance the viability of corresponding microorganisms in the samples.so as to facilitate the short-term preservation of the samples during transport.

Virus sampling tube Virus Transport Medium

Virus sampling tube_Virus Transport Medium

 Method of use

1. Before sampling, mark the relevant sample information on the label of the sampling tube.

2. Sample with corresponding sampling swab.

3. After sampling, quickly put the swab into the sampling tube.

4. Break the swab at the breaking point of the swab and discard the tail.

5. Tighten the pipe cover. Then put the sampling tube containing the sample into the biosafety bag and submit it for inspection in time.

  Features

1. Cell medical’s virus transport medium can preserve almost all cell morphology, structure and quantity.

2. The remaining cells after production can be further directly tested for HPV, DNA, chlamydia, gonorrhea, immunohistochemical tissue test and other tests.

eliminate most bacteria and microbial activities within 3 and 15 minutes. Avoid possible injury to medical staff.

Viral Transport Medium China

Viral Transport Medium China Manufacturer

Viral Transport Medium China manufacturer-Virus sampling tube

Viral Transport Medium is also called virus sampling tubes.

Cell medical not only produces sampling swabs, but also virus sampling tubes, general bacterial sampling tubes and sterile normal saline sampling tubes,Virus delivery medium swab, disposable sampling swab.

The factory is located in Dongguan and belongs to a high-tech enterprise.

Our virus sampling tubes are exported to developed countries in Europe and America and more than 100 countries and regions around the world.

The production capacity of  Viral Transport Medium is very strong, with a daily output of 400000 sets of virus sampling tubes.

Viral Transport Medium Package specification

2ml, 5ml, 10ml, etc.

Intended use

For the transportation and storage of clinical samples.

Inspection principle

Hank’s balanced salt to build a neutral environment;

As a protein stabilizer, bovine serum albumin and Hank’s equilibrium salt increase the survival time and infection stability of the virus;

Antibiotics have broad-spectrum antibacterial effect;

Phenol red is an acid-base indicator.

The pH value of the discolouration area is 6.6 (yellow) ~ 8.0 (red), and it is red when the pH value is 7.2 ~ 7.4.

Limitations of test methods

For the transportation and storage of virus samples.

Product performance index

Appearance: the sampling liquid in the tube is red, transparent and no precipitation liquid;

PH value: when stored at 25 ℃, the pH value is 7.2 ~ 7.4;

Loading capacity: no less than the marked capacity;

Stability: the product shall maintain the stability of various properties within one month beyond the validity period.

Sample requirements

The samples shall be transported to the corresponding laboratory within 2 working days after collection, and the storage temperature shall be 2-8 ℃; If it cannot be delivered to the laboratory within 48h, it shall be stored at – 70 ℃ or below, and the collected samples shall be delivered to the corresponding laboratory within 1 week. Specimens should avoid repeated freezing and thawing.

Main components

Hank’s equilibrium salt, phenol red, bovine serum albumin, gentamicin, polymyxin B, cryoprotectant, etc.

Storage conditions and validity period

Storage condition: 5-25 ℃, validity: 12 months

Viral Transport Medium China Manufacturer

Viral Transport Medium China Manufacturer

Usage

1. Collect samples with a sampling swab according to the sampling requirements.

2. Place the sampling flocked swab after collecting the sample into the sterile sampling tube.

3. Break the swab at the easily broken cut-off point of the sampling swab rod and discard the tail.

4. Tighten the sterile sampling tube cover.

5. Label the sterile sampling tube with information as required.

6. The samples shall be transported to the corresponding influenza monitoring network laboratory within 2 working days after collection, and the storage temperature shall be 2-8 ℃; If it cannot be sent to the laboratory within 48h, it shall be stored at – 70 ℃ or below, and the collected samples shall be sent to the corresponding network laboratory within 1 week. Specimens should avoid repeated freezing and thawing.

7. Specific sampling methods can refer to the following:

1) Pharyngeal swab specimen: use a special sampling swab to wipe the posterior pharyngeal wall and tonsils on both sides with moderate force, and avoid touching the tongue; Quickly immerse the swab head into the sampling solution, break the swab at the easily broken cut-off point of the sampling swab rod, and discard the tail. (applicable to a sampling of this product)

2) Nasal swab specimen: use a special sampling swab, gently insert the swab head into the nasal palate in the nasal canal, stay for a moment, and then rotate and exit slowly; Quickly immerse the swab head into the sampling solution, break the swab at the easily broken cut-off point of the sampling swab rod, and discard the tail. (applicable to a sampling of this product)

Precautions

1. Forbidden to directly contact the sampled liquid with the collected personnel.

2. Forbidden to infiltrate the swab with sampling solution before sampling.

3. Viral Transport Medium is a disposable product.

Only used for the collection, transportation and preservation of clinical virus samples.

4. When collecting virus samples, professionals should strictly follow the sampling procedures;

5. The samples shall be transported to the corresponding laboratory within 2 working days after collection, and the storage temperature shall be 2 ~ 8 ℃;

Specimens should avoid repeated freezing and thawing.

References

1. WHO Global Influenza network monitoring program

viral transport medium tube

Viral Transport Medium Tube

Viral Transport Medium Tube

Whether nucleic acid detection or virus detection, Viral Transport Medium Tube and Sampling Swab play a great role.

Sampling swab is used to collect virus samples, while virus preservation solution plays the role of preserving these virus samples.

Which not only ensures the integrity of nucleic acid virus, but also facilitates the later detection accuracy.

How many virus preservation solutions are there? What are the functions and differences?

At present, there are two kinds of virus preservation solutions on the market, namely inactivated virus preservation solution and non inactivated Viral Transport Medium.

Non-inactivated  Viral Transport Medium can protect the protein and nucleic acid of the virus.

Inactivated Viral Transport Medium usually contains the cleavage salt of inactivated virus to cleave the protein and protect the nucleic acid.

Specific uses of virus sampling tube:

1. The virus sampling tube is generally used for nucleic acid extraction and later virus isolation of influenza virus, hand, foot and mouth virus and rubella virus in clinical patients. The amount of liquid required is usually 35m or 5m. (with nucleic acid detection kit and spore culture medium)

2. For the collection and short-term transportation of avian influenza virus in the external environment. The amount of liquid required is usually 6ml.

3.  For daily monitoring and sampling of poultry, pigs and other animals. The amount of liquid required is usually 15m.

4. For short-term preservation of virus samples. (2-8 ℃ for 48 hours) for necessary cell culture.

5. Used for long-term preservation of virus samples (- 70 ℃ – 196 ℃ for one year) for necessary cell culture.

6. To collect clinical respiratory virus rapid detection kit.

7. To collect clinical samples of mycoplasma, chlamydia and Ureaplasma.

8. To transport tissue samples from the sampling site to the detection laboratory for necessary cell culture.

viral transport medium with swab

Viral Transport Medium with Swab

viral transport medium with swab | COVID-19 sampling and collection kits

The epidemic has disrupted the whole world and affected many industries.

During the outbreak of  COVID-19, people were paying close attention to the epidemic situation while doing personal protection. Novel coronavirus pneumonia and influenza were the most frequent period of communication in winter and spring. The most important way to control the epidemic is to select new patients with new crown pneumonia from many respiratory patients.

About viral transport medium with swab.

Swabs and virus transport media are a transport and collection system for long-term freezing, transport and collection of clinical specimens containing viruses.

The main species of organisms usually transported through this medium include chlamydia, urea plasma organisms and mycoplasma.

With the outbreak of COVID-19 pandemic, swabs and virus transport mediums were used to freeze and collect SARS-CoV-19 virus samples.

Rub a swab on the skin or vesicles to collect virus samples. The collected samples are then sent to the laboratory with transport media.

Why do swabs need virus transport media

After virus collection, the traditional method can not be detected by PCR immediately. The virus itself will quickly dissolve in vitro and affect subsequent tests.

viral transport medium with swab -vtm kits

viral transport medium with swab -vtm kits

Purpose of virus transport media:

1. For monitoring and sampling infectious pathogenic microorganisms by disease control departments and clinical departments. It is applicable to the sampling of influenza virus (ordinary influenza, highly pathogenic avian influenza, influenza A H1N1 virus, etc.), hand, foot and mouth virus and other types of viruses.

2.For the sampling of mycoplasma, chlamydia and Ureaplasma.

3.. To transport nasopharyngeal swab samples or tissue samples at specific parts from the sampling site to the detection laboratory for PCR extraction and detection.

4.To preserve nasopharyngeal swab samples or tissue samples at specific sites for necessary cell culture.

Precautions for sample transportation.

1. Inspection time and temperature control:

After samples sampling, for inspection as soon as possible and sent to the laboratory within 2 ~ 4H as far as possible.

Transport at 2 ~ 8 ℃, and the transport time shall not exceed 72h. If it exceeds 72h, stored and transported at – 70 ℃ or lower.

2. Viral transport medium transport container:

Virus transport tube:waterproof, damage proof, leak proof, high temperature (low temperature) and high pressure resistant. 
Tube body: biological hazard signs, warnings and reminders with relevant regulations. 
Transport container: three-layer packaging system, namely inner packaging, middle packaging and outer packaging.

3. Transportation in the hospital:

The specimen delivery personnel shall be provided with secondary protection and carry 75% ethanol with them.

4. Long distance transportation:

The transport package of 2019 ncov specimens belongs to class A, and the corresponding UN number is un2814. The transporter shall wear safety protection according to the requirements of class II protection, and carry 75% ethanol with him. Drivers wear surgical masks or N95 masks and transport them by special vehicles. If transported by air, the packaging shall also comply with the pi602 classified packaging requirements of ICAO document Doc9284-AN / 905 technical rules for safe air transport of dangerous goods. At least one specimen transporter and driver shall transport specimens at the same time, and monitoring facilities for specimen transport process shall be equipped.

Contact us

  • Cell medical (Dongguan) Co., Ltd.
  • skype:+86-18814476009
  • E-mail:flockedswabs@gmail.com
  • No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China
  • Web:www.swabmanufacturer.com/

 

COVID-19 Sample Collection and Transport kits

COVID-19 Sample Collection and Transport kits

COVID-19 Sample Collection and Transport kits

Since the outbreak of the new crown, we have received many customer queries about which types of swabs, media and kits can be used for sample collection and transportation of COVID-19.

Cell medical (Dongguan) Co., Ltd. produce flocked swabs, VTM kits, virus transport medium, with multiple automatic production lines.

We support custom production, OEM and so on, and develop products with customers.

Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.

Our VTM kits are manufactured in accordance with CDC and FDA guidelines.

Now let’s learn about sample collection and transport kits.

COVID-19 Sample Collection and Transport kits

COVID-19 Sample Collection and Transport kits

How do we collect COVID-19 samples?

Specimen collection requirements

Personal protective equipment (PPE) requirements for sampling personnel: N95 and above protective masks, goggles, one-piece protective clothing, double-layer latex gloves and waterproof boots; In case of contact with the patient’s blood, body fluids, secretions or excreta, replace the outer latex gloves in time.

Specimen type

1. Upper respiratory tract specimens: including pharyngeal swab, nasal swab, nasopharyngeal extract, etc.

2. Lower respiratory tract specimens

3. Stool samples: Patients with diarrhea symptoms need to collect stool samples.

Specimen collection method

Sampling flocked swab_Sample Collection

Sampling flocked swab_Sample Collection

1. Pharyngeal swab: wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with 2 plastic rod swabs planted with pile at the same time, immerse the swab head into a tube containing 3ml virus preservation solution (isotonic salt solution, tissue culture solution or phosphate buffer can also be used), discard the tail, and tighten the tube cover.

2. Nasal swab: gently insert a plastic rod swab with a flocking nylon faucet into the nasopalatine part of the nasal canal, stay for a moment, and then slowly rotate and exit. Take another plastic rod swab with polypropylene fiber head and collect the other nostril in the same way. Immerse the above two swabs into the same tube containing 3ml sampling solution, discard the tail, and tighten the tube cover.

What kind of Media do you use to transport specimens?

Since the outbreak of COVID-19, the Virus Transport Medium has played a major role. Sample and nasal swabs are used to protect samples in the sample tube with virus preservation, which improves the accuracy of detection. Virus preservation solution is a solution for virus sampling, preservation and detection. What kinds of virus preservation solution are added to the virus sampling tube?

virus transport medium

virus transport medium

There are two types of virus preservation transport Media: inactivated and non inactivated.

The following briefly introduces several principles for the selection of virus preservation solution.

Non inactivated virus transport medium:

The non inactivated type does not contain lysate.

On the basis of Hank’s, BSA (bovine serum albumin) amino acids, vitamins and other nutrients required by the virus are added, which can maintain the activity of the virus in a wide temperature range and maintain the originality of the sample to the greatest extent.

For nucleic acid extraction and detection of the virus, virus culture and separation.

Characteristics of non inactivated preservation solution:

COVID-19 Sample Collection and Transport kits Use process

COVID-19 Sample Collection and Transport kits Use process

1. It can maintain virus activity for detection or virus isolation;

2. It has no inhibitory effect on subsequent nucleic acid amplification;

3. stored in cold storage(at 2 ℃ ~ 8 ℃ ).

Inactivation virus transport medium:

Inactivation preservation solution can inactivate the virus in the sample by fully mixing the collected sample with virus lysate and virus nucleic acid preservation solution, and effectively ensure the integrity of virus nucleic acid in the sample.

About the preserved viral RNA samples: used in gene detection, enzyme-linked immunosorbent assay (ELISA), PCR detection and so on.

Inactivation preservation solution includes guanidine salt containing and guanidine salt free.

1. Guanidine salt

(1) Instantly cleaved and inactivated to reduce the risk of sampling;

(2) Stored stably at room temperature for 2 weeks;

(3) It can match most nucleic acid extraction reagents.

2. Non refined salt

(1) Inactivated instantaneously to reduce the virus sampling risk, and the virus may recover its infectivity after dilution;

(2) Stored stably at room temperature for 2 weeks;

(3) It can match most nucleic acid extraction reagents.

3. Direct expansion preservation solution

(1)Inactivated instantaneously to reduce the virus sampling risk, and the virus may recover its infectivity after dilution;

(2)Stored stably in cold storage for 24-48 hours;

(3) Directly added to the nucleic acid amplification system as a template;

(4) Match most nucleic acid extraction reagents and nucleic acid release agents.