The virus transport medium is novel and unfamiliar to many small partners, but it is of great significance for the detection of various viruses.
The new crown virus has tortured human beings enough.
In the face of a large number of COVID-19 patients, some places are still rising.
Therefore, as soon as there are new additions, many places will focus on the new crown nucleic acid detection.
The virus preservation solution plays a very important role.
At present, the nucleic acid detection of the new coronavirus is mainly the detection of viral nucleic acid.
Virus transmission medium is a cell preservation liquid, which protects the virus to be detected by immersing the flocked swab head with the virus sample into the transport tube.
It is mainly suitable for the new coronavirus, Collection, preservation and transportation of common virus samples such as the influenza virus.
To collect throat swabs, nasal swab or tissue samples from specific parts.
The stored samples: for subsequent nucleic acid detection or clinical experiments such as virus culture.
Since the virus is a parasitic organism, it cannot survive outside the body after sampling.
If it cannot be detected in time, it needs to be placed in a virus preservation solution for preservation. (Virus Transport Medium)
In order to protect the safety of the virus detection environment and the safety of the detection personnel, inactivated virus preservation solution is actually a better choice!
For different detection purposes, we need to use different virus preservation solutions for preservation.
The two widely used preservation solutions have their own characteristics.
In order to meet different detection requirements and different experimental conditions for virus detection, it is necessary to use different preservation solutions.
The inactivated virus preservation solution can inactivate the virus.
How to detect it after inactivation?
The purpose of our use of inactivated preservation solution is to lyse nucleic acids, release nucleic acids and then carry out nucleic acid detection by subsequent real-time fluorescent RT-PCR.
Doctors use inactivated virus transport media to determine whether the sample contains virus characteristic nucleic acid, that is, whether we carry the virus.
Inactivated virus transport medium:
A virus lysis type preservation solution improved by nucleic acid extraction lysis solution.
Effectively prevent the secondary infection of the operator, but it also contains inhibitors, which can protect the viral nucleic acid from being degraded.
This enables subsequent detection by NT-PCR.
Moreover, stored at room temperature for a relatively long time, which saves the cost of virus sample storage and transportation.
Non-inactivated virus transport medium:
A virus maintenance liquid type preservation solution improved on the basis of the transport medium.
It maintains the activity of the virus in vitro and the integrity of antigens and nucleic acids.
The protective viral protein shell makes it difficult to decompose and maintains the originality of the virus sample to a great extent.
For nucleic acid extraction and detection, the experimenters also use the transport medium for virus culture and isolation and maintain the activity of virus samples.
In addition to nucleic acid extraction and detection, the inspector also uses this preservation solution for virus culture, isolation and antigen detection.