How to Choose a Correct Virus transport medium for COVID-19 Testing
Disposable virus sampling tube
At present, the form of New Coronavirus is still very serious.
As the first step of covid-19 detection, the importance of sample collection and preservation is beyond doubt.
The influencing factor is sample collection; If there is a problem with the collected sample, the result is invalid regardless of the subsequent treatment.
Selecting appropriate virus transport media and collecting flocking swabs can improve the efficiency of new crown detection.
Almost all virus preservation solutions on the market contain non inactivated viruses directly, resulting in high infection risk for sampling, transportation and testing personnel.
Therefore, the front line of epidemic prevention needs to be able to inactivate virus samples directly.
At 37 ° C, we compared the preservation effects of cell medical (Dongguan) preservation solution containing guanidine hydrochloride and guanidine isothiocyanate with that without guanidine salt on avian coronavirus.
After 1-7 days of storage, under the same condition of 37 ℃, the preservation rate of virus nucleic acid was basically maintained at 100% when it was preserved with cell medical (Dongguan) preservation solution;
·Use virus preservation solution that can protect the sample from high temperature inactivation.
The results showed that high temperature inactivation had no significant effect on the integrity of coronavirus nucleic acid when coronavirus nucleic acid samples were preserved with high temperature inactivator.
The virus preservation solution developed by Cell medical (Dongguan) company is divided into inactivated and non inactivated. Different preservation solutions can be selected according to different test requirements and test conditions. If you have any questions or needs, please call us. Choosing cell medical (Dongguan) virus transport media solution in China is your right choice.
Furan compounds (guanidine isothiocyanate or guanidine hydrochloride, etc.) are typical protein denaturants.
They are often used to extract cell lysis in nucleic acid and inactivate virus. However, the furamethoxam component does not have the ability to work at room temperature.
Because the ability to preserve viral nucleic acid is easy to lead to sample degradation, it is not suitable for preserving new coronavirus samples. Therefore, it is strongly recommended that you choose the preservation solution without guanidine salt.
To this end, we call on the majority of medical personnel to select non inactivated virus transport media if they take samples for the purpose of nucleic acid detection and do not need to culture live viruses.
Vtm Swab | Virus Transport Medium with Sampling Swab
Use a virus medium that inactivates the virus.
At present, covid-19 detection is mainly to detect viral RNA, which needs to divide the virus.
Therefore, as long as the integrity and stability of virus nucleic acid can be guaranteed, there is no need to preserve live virus.
Therefore, in the current situation of fighting COVID-19, it is ideal to inactivate the virus while collecting samples. Some manufacturers have also introduced inactivated transport medium, but its inactivating component is guanidine salt.
The results showed that the salt forming component storage solution had insufficient storage capacity for viral nucleic acid at room temperature, which was easy to cause sample degradation.
Therefore, we recommend the use of virus preservation solution containing non guanidine protein denaturants.
The volume of solution in the sampling tube should be appropriate.
① When sampling each sample separately, we recommend that you select a virus sampling tube containing 1ml storage solution to ensure the correct storage capacity. It can not only meet the downstream needs of nucleic acid extraction and detection. Compare with 3ml storage solution. Triple the virus concentration!
② For large-scale preliminary screening and mixing test, we should choose to put the samples of 3-5 people into the sampling tube.
COVID-19 Pooled Sample Testing.
Nasal swab virus sampling kits Virus transport medium for COVID-19
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·Use a virus preservation solution that can transport and preserve samples at room temperature.
If the delivery temperature does not meet the standard, the viral nucleic acid may degrade, resulting in a “false negative” test result!
On the contrary, if cell medical uses a virus storage solution that can transport and stably store virus nucleic acid at room temperature.
We solved the problem of sample transportation and improved the accuracy of test results.
·Use virus preservation solution that can protect the sample from high temperature inactivation.
However, the research reports of Beijing Center for Disease Control and prevention in the Journal of “clinical chemistry” and Beijing Preventive Medicine Research Center show that high temperature inactivation can lead to the degradation of virus nucleic acid, thus reducing the detection rate of virus nucleic acid. Therefore, The false negative rate of New Coronavirus nucleic acid detection is high.
The Virus transport medium for COVID-19 developed by cell medical (Dongguan) Co., Ltd. include： inactivated and non inactivated.
If you have any questions or needs, please call us.
Choosing cell medical virus transport media solution in China is your right choice.