Viral transport media for COVID-19 testing

Posted on 2022年3月25日2022年3月25日 by Dr. Lee

At present, medical technology needs to promote positive energy!

The current epidemic has come and gone.

In particular, important medical materials such as disposable virus sampling tubes (virus transport media) play a key role.

At present, the quality of the products of various manufacturers on the market is also uneven, and there is also a risk of affecting the accuracy of the experimental results.

Make products with a realistic and rigorous scientific attitude;

Let the front-line medical staff have no worries, and provide them with a steady stream of “cannonballs”.

Below we will define the virus delivery medium and explain typical components and options for use.

What is a virus transport medium?

10ml Viral Transport Medium VTM Swab Tube Kit

It is suitable for the collection of various influenza viruses, avian influenza viruses, COVID-19 novel coronavirus, hand, foot and mouth virus samples.

Realize the lysis and inactivation of the virus in the sample.

At the same time effectively ensure the stability of the viral nucleic acid in the sample during storage and transportation at room temperature.

And collect the sample for subsequent PCR analysis and detection.

Features:

✳ Room temperature stable storage;

After use, Stored at room temperature for 7 days, which is conducive to the collection and transportation of samples.

✳ Quick release

Using swabs to collect samples is conducive to subsequent nucleic acid release.

✳ Safe and rapid inactivation of virus samples

The preservation solution can quickly inactivate virus samples, effectively reducing the level of biosafety requirements for sample transportation and subsequent testing laboratories.

✳ Swab breakpoint design

After sampling, the designed rod breakpoint design can easily break the head and save it in the tube.

What are the components of the viral transport medium?

The sample transport medium, viral transport medium (VTM), stabilizes cells and viruses in potentially infected patient samples during transport from the point of collection to the testing facility.

These reagents are suitable for various types of viruses, including coronaviruses, herpes viruses, and influenza viruses.

CellMedical’s products for sample transport include viral transport media and saline solutions, which are both efficient collection and transport media for subsequent qPCR assays.

All sample transfer media are manufactured in a state-of-the-art GMP facility certified to ISO 13485:2016.

UTM Tube with NP Flocked Flexible Swab

1. Virus sampling tube;

Some data suggest that polypropylene (Polypropylene) is related to the adsorption of nucleic acids, especially at high tension ion concentration, polyethylene plastic (Polyethylene) is easier than polypropylene (Polypropylene) to grasp DNA/ RNA.

In addition, when using breakable swabs, the storage tube should try to choose a container with a height of more than 8 cm to prevent the contents from splashing.

Some tubes may be formulated to contain buffered proteins and antibiotics to inhibit the growth of potentially contaminating bacteria and fungi.

2. Virus Transport Media;

VTM consists of Hanks Balanced Salt, Foetal Bovine Product, anti-bacterial prescription antibiotics, anti-fungal anti-biotics as well as Phenol Red as an indication for real-time surveillance of pH security of the service.

3. Collection of flocked swab;

The sampling swab directly contacts the sampling site, and the material of the sampling head is closely related to the subsequent detection.

Sampling swab tips should be made of Polyester (PE) synthetic fibers or Rayon (man-made fibers).

Prohibit calcium alginate sponges or wooden stick swabs (including bamboo sticks).

For example, the material of the swab tip cannot be cotton.

Products, because cotton fibers have strong adsorption of protein.

It is not easy to be eluted into the subsequent preservation solution.

Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.

Viral transport media applications

The virus sampling tube is a complete set of centrifuge tubes for sampling and transporting viruses such as influenza virus, hand, foot and mouth virus, covid test, etc. in the microbial sampling transport tube, also known as the specimen transport tube.

In the current epidemic, virus sampling is also an important part. Generally, viruses exist in large quantities in the nasopharynx of the human body.

The pharyngeal tester collects virus samples from the nasopharynx and puts them in a virus sampling tube.

Nasopharyngeal (NP) Swab VS Oropharyngeal Swab

Posted on 2022年3月25日2022年3月25日 by Dr. Lee

The new crown epidemic is a matter of public defence that we can’t avoid and can’t hide from.

I believe everyone knows the impact of the new crown epidemic on us.

I believe you all know the impact of the new crown epidemic on us.

As the number of COVID-19 cases across the country starts to climb again.

Everyone has started to discuss it again, and I believe that no topic can attract the attention of the world for such a long time like our current global new crown virus.

Some of us have raised such a question, why do we have two detection methods for nucleic acid detection points.

One is for extraction in the nose, and the other is for extraction in the mouth?

Nasopharyngeal swabs and oropharyngeal swabs only take different paths.

The oropharyngeal swab is to collect oropharyngeal specimens by entering the sampling swab through the mouth.

The nasopharyngeal swab is to collect nasopharyngeal specimens through the nose.

The is a matter of public defence that we can’t avoid and can’t hide from.

I believe you all know the impact of the new crown epidemic on us, as the number of COVID-19 cases across the country starts to climb again.

Everyone has started to discuss it again, and I believe that no topic can attract the attention of the world for such a long time like our current global new crown virus.

Some of us have raised such a question, why do we have two detection methods for nucleic acid detection points, one is for extraction in the nose, and the other is for extraction in the mouth?

Nasopharyngeal swabs and oropharyngeal swabs only take different paths.

The oropharyngeal swab is to collect oropharyngeal specimens by entering the sampling swab through the mouth.

The nasopharyngeal swab is to collect nasopharyngeal specimens through the nose.

Nasopharyngeal swabbing is generally the preferred method of specimen collection for COVID-19 testing.

model

composition

the difference

use

VTM-A-CM0403

Flocked swab (1 nose and pharynx), sampling tube (including 3ml preservation solution), biosafety bag, instruction manual.

Non-inactivated viral transport media maintains viral activity and antigen and nucleic acid integrity.

Preservation and transport of viruses, nucleic acid extraction and detection, etc.

VTM-B-CM0403

Sampling flocking swab (1 nose and pharynx), sampling tube (including 3ml preservation solution), biosafety bag, instruction manual.

Inactivated virus transport medium, colourless clear liquid.

The virus has been inactivated and is mainly used for nucleic acid detection and extraction,

2. How to effectively collect nasal samples for patients

Place the entire tip of the swab into the nose and rub one side of the swab as much as possible against the wall of the nostril (also called the anterior nostril) with moderate pressure.
Use the same cotton swab to do four to five sweeps in each nostril for about 10-15 seconds per nostril. Both nostrils should be swabbed in order to collect an adequate sample.
Simply swirling the swab into the inner part of the nose or leaving the swab in the nose for 10-15 seconds is an inappropriate technique and may result in insufficient sample.

3. Features and advantages of disposable virus sampling tube (pharyngeal swab)

High safety: medical materials, good airtightness, good stability, prevent leakage and ensure biological safety
Various options: nasal/pharyngeal swab + inactivated/non-inactivated preservation solution to meet different experimental needs
High-efficiency inactivation: high-quality formula can inactivate viruses quickly and efficiently, avoiding the risk of aerosol infection
Nucleic acid stability: The preservation solution contains special stable components to ensure the stability and integrity of viral nucleic acids during in vitro storage and transportation
Easy to operate: independent disposable packaging, no need to refrigerate, and can be transported at room temperature

4. About flocked swabs

The total length is 15CM, the breaking point of the throat swab is 3CM, and the breaking point of the nasal swab is 8CM;
Virus sampling for oral and nasal cavity;
Unique jet nylon villi implantation technology increases sample collection and release;
No sample residue, complete and rapid release of samples;
15cm lengthened processing, easy to obtain materials, avoid the risk of contamination;
Individually aseptic packaging for individual servings for easy handling and transportation;

5. About us

Cell medical (Dongguan) Co., Ltd., a professional VTM kits and swabs manufacturer, disposable swab tubes exporter, has many years of experience in medical application moulding and flocking treatment heads and swabs.

Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.

Certificate:FDA,CE,EU,Free Sale,Export license based on (GMP).

We produce flocked swabs, VTM kits, Virus transport mediums, with multiple automatic production lines.

We support custom production, OEM and so on, and develop products with customers.

How Accurate Is The Nasal Swab

Posted on 2022年3月24日2022年3月24日 by Dr. Lee

Swab test has methods such as nasal swab and pharyngeal swab.

Which is more accurate, nasal swab or pharyngeal swab?

Which of the two is more painful? If you want to know, let’s have a look.

Which is more accurate to detect a poke in the nose or a poke in the throat？

Theoretically, under the condition of standard operation, the results and accuracy of nasal swabs and pharyngeal swabs will be the same.

However, in the actual clinical work and the reagents for collecting samples, nasal swabs and pharyngeal swabs need to collect the secretions on the respiratory mucosa, which are irritating to some extent.

In particular, pharyngeal swabs are prone to nonstandard phenomena.

For example, without a tongue depressor, the pharynx of the subject is more sensitive and cannot collect qualified samples.

In addition, the throat swab needs the collector to open his mouth, which is easy to form aerosols and pollute the surrounding environment.

This will not ha ppen during the collection of nasal swabs.

In contrast, the results will be more accurate.

Nasopharyngeal swab and throat swab which is painful？

Depends on individual tolerance.

In the collection of nucleic acid sample detection samples through nasal swabs and throat swabs.

There will be a certain discomfort, but this discomfort is within the tolerable range.

However, compared with the two, nasal swabs may be less irritating to the mucous membranes, and the discomfort caused by standardized operations is less,

Collected through the nasal cavity, the public’s acceptance of them will be lower.

Can I do a swab test for a sore throat?

Yes.

Nucleic acid testing can also be performed during throat inflammation, which will not affect the results of the nucleic acid test.

It’s just that symptoms such as acute pharyngitis, acute tonsillitis, acute epiglottitis, etc., in the process of nucleic acid detection, have a certain degree of irritation to the mucous membranes of the throat, which can easily aggravate throat discomfort.

Recommended to inform the medical staff in advance before nucleic acid sampling.

Can I eat before the nucleic acid test

Avoid eating.

Avoid to eat 2 hours before the nucleic acid test, because when collecting nucleic acid samples, it is usually necessary to collect secretions from the throat or nasal cavity. Because the cotton swab will irritate the throat during nucleic acid test sampling, it can cause nausea and throat of the patient.

Symptoms such as discomfort, if you eat too much, it is easy to cause vomiting.

In addition, before the nucleic acid test, be careful not to smoke or drink alcohol, and it is best not to chew gum, otherwise the accuracy of the nucleic acid test may be affected.

What is a Flocked Swab Test

Posted on 2022年3月23日2022年3月23日 by Dr. Lee

What is a Flocked Swab Test

CellMedical is in the spotlight as a key manufacturer in the COVID-19 testing supplies supply chain.

In the face of the outbreak, demand for this flocked swab and its transport medium has skyrocketed, quickly depleting supplies across the country.

Cell medical (Dongguan) Co., Ltd. is a company with more than 10 years of professional development, production and sales of flocked swabs, DNA flocking swabs, oral swabs. Disposable sampling swabs. Disposable swabs sub. virus sampling swab. single-use virus sampling tube. Virus transport medium.

Flocked swab factory

We have our own independent laboratory and production base.

At present, our main business includes sponge cotton swabs, flocking cotton swabs, flocking swabs, sampling cotton swabs, sampling cotton swabs, sampling swabs, medical cotton swabs, medical applicators, polyester-cotton swabs and microfiber cotton swabs.

A purifying cotton swab product.

All our products are produced in a 10,000-level GMP cleanroom.

Our GMP manufacturer

And the production process is all carried out in accordance with the relevant GMP dust-free production specifications, which maximizes the quality of the products.

We currently manufacture over 1,200 different types of swabs and collection devices for a variety of industries, typically producing over 12 million swabs per day.

We are rapidly adjusting some of our manufacturing capabilities to meet the demand for COVID-19 testing materials.

Product Usage:

Nylon flocked swabs are versatile and are useful in bacteriological sample processing, covid swab tests, virological cell cultures, DFA testing, rapid and direct testing, polymerase chain reaction and molecular diagnostic-based tests, and forensic identification. ideal.

For throat sampling of respiratory viruses such as influenza, swine flu, bird flu, hand, foot and mouth.

Sampling swab product features:

1. It has an extraordinary water absorption capacity, which can increase the number of specimens collected on its surface from 20% of traditional sampling swabs to 60%;
2. Has a release rate of over 90% for the collected specimens, thus ensuring a high degree of reliability of the results;
4. There is a unique breakable design on the plastic rod, which is convenient for specimen transportation;
5. Ethylene oxide sterilization, irradiation sterilization, independent packaging;

Gene sampling series flocked DNA oral swabs are specially designed to improve the yield and quality of genomic DNA in oral swab cells. Oral cell samples are a good alternative to blood for non-invasive collection and detection.

DNA flocking swabs are suitable for human and animal use and have advantages over other DNA swabs in design. Its unique swab matrix and rapid release interface are more conducive to efficient cell collection and maximising DNA yield. The swabs have been sterilized with ethylene oxide and tested for human DNA contamination.

Application

Genotyping studies;

Veterinary Genotyping and Diagnostics;

Parenting and Genetic Services;

Forensic and DNA population research;

How to Collect DNA Buccal Swabs

Posted on 2022年3月19日2022年6月2日 by Dr. Lee

DNA can tell you a lot about you.

DNA sample collection DNA is collected from an individual (or individuals) using sterile swabs while following appropriate collection techniques.

A DNA test can do this by looking at your DNA to determine the DNA “function” it exposes in your genetic code.

This is the “feature” that some tests can provide for health and life.

The reason for the method information.

Under the license of numerous DNA companies, some will sequence your DNA through your clients to help you understand your family tree, the migration patterns of your ancestors.

Proper DNA sample collection techniques are an essential skill for forensic scientists, crime scene investigators, and medical examiners. We can collect DNA samples from many different sources.

Products found at a criminal crime scene that may not actually involve a true crime may provide DNA evidence.

What is a DNA test and how is it done?

Use your doctor’s genetic sample to test you or be asked to spit in a test tube , or be asked to flocked swab in a tube in your mouth.

If you have frequent dry mouth, you need to do a cheek test. Another could be a lemon bite, the taste of a lemon, and then a bite of the lemon. When to get those drools back. Besides, you can also learn a little more but about DNA testing things!

DNA sample collection method

: generally choose routine: bloodstain, oral test, long sample;

Swab collection method:

Rinse your mouth with water to make sure there is no food residue in your mouth.
Open the DNA storage solution tube. Turn the cap down and the spout up.
Unwrap you and stick your fingers around the probe. It is important not to probe with a profiler of a profiler, as this can affect the profiler results.
Insert a medical DNA swab into the mouth and rub 30 times on the cheeks.
Take a cotton swab and dry the swab in the shade; use the same method to collect three DNA subs.

Exactly how to prevent DNA evidence contamination

When managing all organic evidence for DNA screening, it is imperative for investigators and laboratory staff to wear the correct personal safety equipment, use tidy instruments, and avoid interactions with various other objects to reduce the threat of compromise of any evidence.

Improper storage space, direct exposure to direct sunlight, or simply being comfortable can all cause damage to DNA. To avoid damage, keep accumulated evidence in a dry and cool place and send it to the laboratory immediately.

What is the source of DNA you can wipe?

You can easily wipe down many common DNA sources. These include blood, feces, urine, semen, saliva, hair, teeth, bones, and cells. DNA can also accumulate from selected surfaces and materials.

Where can DNA samples be obtained?

DNA samples can be extracted from drinking glasses, cigarette butts, food with bite marks, bite marks on the skin, etc. You can also wipe down frequently touched surfaces such as computer keyboards, doorknobs, steering wheels, glasses, and countertops.

Flu Test Swab

Posted on 2022年3月18日2022年3月30日 by Dr. Lee

The COVID-19 pandemic has actually added another layer of complexity to the flu season ahead of schedule. COVID and the flu share many similar signs and symptoms, which certainly makes diagnostic screening even more important this flu season.

What is a flu swab test?

Flu is the abbreviation of Influenza. This disease is not unfamiliar to us. It is an acute respiratory infection caused by influenza virus.

Influenza is very contagious and spreads very quickly. Once you have flu symptoms, you must go to the hospital for relevant examinations in time to determine the type of flu before symptomatic treatment.

Every year when the seasons change, the virus will also increase due to changes in temperature. This period is also the time when we are most prone to getting sick.

Among them, influenza is a very common epidemic infectious disease. Influenza is familiar to most people, but not very well known to a few people.

Getting a quick, accurate diagnosis is of course critical to driving treatment decisions.

Influenza testing can be done with blood tests, nasopharyngeal swabs, etc.

How to Test for Flu with a Swab.

Which Swab Test is More Accurate?

Discovering the right flu test is critical to ensuring accurate results. Using high-quality items—including appropriate swabs, shipping media, and testing devices—can help to properly identify influenza and reduce the potential spread of infection. Influenza testing needs to detect the influenza virus.

After infection with influenza, the influenza virus usually invades the nasopharynx first, which can be detected by a nasopharyngeal swab.

Influenza virus can also enter the blood and can be detected by a blood test. Influenza is a relatively serious respiratory infectious disease.

After the infection, the systemic symptoms are more obvious, such as high fever, general weakness, and sore limbs.

The recovery of the disease is also relatively slow, and it takes about half a month.

Each of the influenza tests listed above can be performed using a nasopharyngeal (NP) swab, throat swab, NP swab, nasal or endotracheal aspirate, or saliva test.

Covid Test Throat Swab

To perform an NP flu swab test, follow these steps:

Choose a flocked swab with excellent absorption and elution, such as CellMedical’s Nasopharyngeal Swab CM-96000N 6″ Sterile Flocked Swab with Breakpoint 90mm.

With the patient’s head tilted back, slowly insert the flocked swab into the nasal cavity.

Place the swab directly into the nostril directly back (not up) and at the level of the nasopharynx to the distance determined on the swab handle.

Rotate the swab approximately 3 to 5 times and hold in place for 5 to 10 seconds to obtain a sample.

Remove the sampling swab and insert into a virus sampling tube containing 1 to 3 ml of virus transport medium.

Break the flocked swab along the breakpoint.

Label the vial with the appropriate customer information.

How long does a flu swab test take?

Throat swab test belongs to a class of medical test methods. A medical sampling swab is used to take a small number of secretions from the pharynx of the human body, inoculate it in a special petri dish, and then put it into a temperature control device for cultivation. Throat swab test can understand the patient’s condition, oral mucosa and throat infection. As for how long the results will take, depending on the number of people tested, some hours, some a day, some a week.

How accurate is the flu swab test?

The flu nasopharyngeal swab test is more accurate when the virus load is sufficient.

This is used to determine whether the human body has the flu.

But if the test comes back positive, that would require sampling and testing of those who came into contact with the patient.

It can prevent the spread of influenza, and it is necessary for suspected patients to be properly isolated, placed separately, and tested.

Nasopharyngeal swabs are commonly used clinically to detect viruses.

That is, take a sampling flocked swab and gently wipe the patient’s nasopharynx, and then take samples for testing.

Ethylene Oxide Covid Test Sterile Swab

Posted on 2022年3月17日2022年3月30日 by Dr. Lee

EO sterilization refers to ethylene oxide sterilization.

Ethylene oxide (EO) is a highly efficient and broad-spectrum gaseous disinfectant.

It is by far the only most reliable and widely used sterilization method recognized worldwide.

The nasal sampling flocked swabs included in the COVID-19 test kits are sterilized by EO or gamma-ray sterilization.

Rest assured, the swab is safe to use. Including ethane is a gas use commonly used to sterilize different types of medical equipment, flocked swabs for test kits.

The time period for the entire process includes multiple heights and set-up phases, including post-swab removal of sterilized equipment to control ethane treatment.

Allows monitoring of residues in contact with humans (EO amount has been according to ISO 10939-7:2008) ).

Our GMP Factory Flocked Swab Ethylene Oxide Covid Test

Ethylene Oxide Covid Test Sterile flocked Swab Principle

What is the principle of ethylene oxide sterilization (EO sterilization)?

Ethylene oxide, also known as ethylene oxide, has the molecular formula C2H4O.

The boiling point is 10.4°C, it is soluble in water, and the explosion limit in air is 3% to 100% (volume).

Ethylene oxide is a highly volatile, explosive, flammable and toxic substance with ether smell when volatilized. It is a spectral sterilant.

Ethylene oxide can effectively kill various microorganisms, including bacterial propagules, spores, viruses and fungal spores, and is a broad-spectrum high-efficiency sterilant.

Ethylene oxide molecule can produce alkylation reaction with a carboxyl group, amino group, sulfhydryl hydrogen group and hydrogen group on gene proteins necessary for metabolism in bacterial cells.

Instead of the unstable hydrogen atoms on the above groups, form a hydroxyethyl group.

The rooting compound, because this compound destroys the necessary reactive groups in the important metabolic reactions of microorganisms, affects the action of bacterial enzymes, and causes the death of microorganisms.

Nasal swabs included in COVID-19 inspections are EO sterilized.

I heard that ethylene oxide sterilant is toxic and carcinogenic, but covid sterile swaps are all sterilized with ethylene oxide.

Is it safe for us to use a covid swab?

Is the risk of cancer caused by directly entering the respiratory tract greater?

The words “Clean and Sterile EO” on the product packaging of sterile flocked swabs. At this point there’s a crowd that’s saying “

Specimen Collection Swab Ethylene Oxide In Swabs

It’s completely dry here.

There’s no result. But once you put it in a person’s nose, it’s wet. Once you inhale it, it’s going to kill us. It’s really killing people.”

While it’s safe to say that direct exposure to ethylene oxide is actually linked to cancer (here), that doesn’t suggest that swabs sterilized with ethylene oxide definitely cause cancer cells.

Ethylene oxide is a valuable disinfecting gas because it is very good at inactivating bacteria.

It is used in factories in a very tightly controlled method to ensure that people working in manufacturing plants are not put at risk.

It is a common chemical used to sterilize clinical tools and leave a minimal amount of product behind.

In conclusion, EO is a gas commonly used to sterilize clinical equipment such as COVID-19 nasal swab tests.

Ethylene oxide (ethylene oxide) is a highly efficient and broad-spectrum gaseous disinfectant.

It is by far the only most reliable and widely used sterilization method recognized worldwide.

Although EO is carcinogenic, the use of this gas is safely regulated and has actually been used for decades.

Sterilization procedures are regulated to ensure minimal EO residues on medical tools.

Np Swab Covid Test Meaning

Posted on 2022年3月16日2022年4月7日 by Dr. Lee

Np Swab Covid Test Significance Np Swab is the English abbreviation of nasopharyngeal swab.

This article provides information on how to properly perform nasopharyngeal swab sampling, how to analyze samples taken from swabs.

Nasal swab? What is the difference between a nasopharyngeal swab and a nasal swab?

Nasopharyngeal Swab Pcr Test

Nasopharyngeal Swab vs Nasal Swab (Anterior Nasal Swab)

What is the method of nasopharyngeal swab?

Collection of specimens from the surface of the respiratory mucosa with nasopharyngeal flocked swabs is a procedure used for the diagnosis of Covid-19.

A nostril sample collection is usually one of the nursing care.

What is the nasal swab technique?

Part of the inspection method is done using sub-diagnostics and collecting diagnostic samples from the membrane. Rotate for 10-5 seconds while touching both walls, then use a cotton swab with 1 mid-hole in one nostril.

In the same way in the other nostrils: usually preferred for people – and used in my own testing. The same goes for technology.

Which swab test is more accurate?

Nasopharyngeal swabs are generally the recommended sample collection technique for COVID-19 testing.

Additionally, a 2012 comparative study showed no significant analytical differences between the two influenza detection technologies.

In addition to being the typical testing approach for the diagnosis of COVID-19.

The nasopharyngeal swab: is likewise regularly utilized for the detection of different viruses and bacterial infections.

How do we perform nasopharyngeal swab sample collection?

Flocked Tip Swab

Healthcare experts need to execute these tests in a specialized and also sanitized room that prevents the spread of the infection.

All healthcare specialists administering the nasopharyngeal swab examination have to wear the suggested personal protective devices (PPE), of which ought to include an N95 mask, non reusable cap, goggles, dress, latex gloves, and also footwear covers.

When the individual sitting upright as well as their head in a straight position, as this provides very easy exposure and also access to the nasal floor, which is vertical to the facility axis of the person’s face.

The staff places the swab into the client’s nose in the same direction as the nasal cavity and diaphragm floor.

As long as there are no obstructions existing within the nasal dental caries, the swab will certainly remain to relocate this instructions until it reaches the nasopharynx.

Once the swab reaches the nasopharynx, finishes two full 360-degree rotations to enable the secretions from this area.

When we take the swab out of the individual’s nose. Immediately insert into a vial with medium.

Nasopharyngeal Swab Procedure.

What can a nasopharyngeal swab find?

As previously pointed out, a nasopharyngeal swab: is extensively utilized to identify (SARS-CoV-2) infection, which is the infection that causes COVID-19 disease.

Identified via nasopharyngeal swabs, several of which include rhinovirus, and adenovirus. flu. respiratory syncytial virus (RSV). (HPIV). (HMPV) as well as (EV).

Given that the nasopharynx is generally home to a high number of both nonpathogenic as well as pathogenic microorganisms. This testing approach can additionally be utilized to detect particular bacterial pathogens.

Such as Streptococcus pneumoniae, Haemophilus flu, as well as Moraxella catarrhalis.

A diagnostics firm, for example, has just recently presented a novel nasal swab that gathers nasal epithelial cells for the early molecular discovery of lung cancer in both current as well as previous smokers.

We obtained nasopharyngeal swab samples, and the current standard for diagnosing SARS-CoV-2 is real-time fluorescent polymerase chain reaction (RT-PCR).

Pcr test nasal swab and an antigen test difference for covid 19

Commonly, the RT-PCR test will measure several various RNA gene targets, such as several of the envelope (env), nucleocapsid (N), spike (S), RNA-dependent RNA polymerase (RdRp) as well as ORF1 genes.

RT-PCR test is an analytical technique suitable for SARS-CoV-2 discovery because of its reliability and affordability.

As well as sensitivity, especially when compared to different analysis techniques like cell culture, antigen discovery, and serological approaches.

There are numerous reasons that negative test outcomes can develop as a result of an incorrect nasopharyngeal swab method.

One instance includes the numerous locations in between the opening of the nose and also the nasopharynx where the screening workers can experience resistance.

Commonly, a knowledgeable caregiver can acknowledge when the sampling swab has actually gotten to 9-10 centimetres into the nasal dental caries.

If the swab does not reach this depth, it has not accurately tasted liquid from the nasopharynx and can for that reason not adequately determine the existence of a disease within this location of the body.

For this reason, it is critical to assess whether employees receive proper education on how to accurately perform nasopharyngeal swab testing.

What is an Antigen Covid Test?

Posted on 2022年3月15日2022年3月15日 by Dr. Lee

Antigen Covid Test

We routinely test the nucleic acid with nasal swabs in the department every week, and we have been used to it for several months.

At most, it will pass with a tear and clenched teeth (naive).

So when I was queuing, I didn’t think there was anything to do mentally, and I didn’t notice that the head nurse was training a new little nurse how to do nucleic acid today‼ ️

As a result, it was my turn, and as soon as I sat down, I heard the head nurse say, “I’ll show you one.”

So before she could react, she stabbed in at once, and that feeling, I have never felt so uncomfortable in my life, from nostrils to throats.

It felt like my head was split open, and tears came out. The nasal swab is about 15cm long. According to my classmates, there are only 2cm left.

And because I was so overreacting (yelling and leaning back so fast), the head nurse was so startled that I didn’t have time to pull it out.

The covid flocked swab didn’t come out even after she let go, and I pulled it out with tears.

Many Americans are familiar with the rapid antigen test for COVID-19, which involves swabbing the nose.

What is an antigen test? Can it replace nucleic acid detection (PCR swab test)?

What is an antigen test?

The new coronavirus antigen detection is detected on a test strip through the binding reaction of antigen and antibody.

which is convenient and quick, and the result is usually available in 15-20 minutes.

However, due to the existence of some interference, the antigen-antibody reaction may be falsely positive.

The specificity of the method is less than 100%.

Therefore, antigen test results cannot replace nucleic acid test results.

There are three main methods to detect whether the virus invades the human body: nucleic acid detection (PCR), antigen detection and antibody detection.

The first two are methods of direct detection of viruses.

Compared with nucleic acid detection, antigen detection can be faster and more convenient to operate.

But with lower accuracy, generally used in the initial stage of infection.

After the outbreak, many countries, while recognizing nucleic acid testing as the “gold standard” for COVID-19 testing, have also promoted antigen testing as an effective supplement.

Antigen-positive results can be used for early triage and rapid management of suspected populations.

1. Why do I need an antigen test?

Facilitates “early detection” The new coronavirus mainly has four structural proteins.

Compared with nucleic acid detection, antigen detection is faster and more convenient to operate, and some reagents can produce results in 15 minutes.

Faster and more accurate epidemic prevention and control requires additional antigen testing as a supplement.

Once the antigen test is positive, we need to carry out nucleic acid testing to confirm in time.

2. Why can’t it replace nucleic acid detection?

or false detection According to the latest plan, nucleic acid testing is still the basis for the diagnosis of new coronavirus infection.

Antigen detection is a supplementary method and cannot replace nucleic acid detection.

The reason is that the sensitivity of antigen detection is relatively low. And antigen detection products can usually only be detected when the infected person is in the acute infection stage.

That is, when the viral load in the body is at a high level.

Compared with nucleic acid detection, antigen detection has poor sensitivity due to no amplification process.

In layman’s terms, it shortens the time for results, but increases the risk of “false negatives”.

According to the above plan, if the primary medical and health institutions have nucleic acid detection capabilities, nucleic acid detection should be the first choice;

If they don’t have nucleic acid testing capabilities, they can do antigen testing.

In the current epidemic, there are many asymptomatic infections.

Can we get through testing?

From the existing data, a large part of the infected people of the Omicron variant are asymptomatic, but it does not mean that their viral load is low.

On the contrary, some studies have shown that the viral load in the throat of some asymptomatic infected people is quite high.

What is Viral Transport Media

Posted on 2022年3月14日2022年3月30日 by Dr. Lee

What is VTM?

VTM is the acronym for Viral Transport Media, which contains nutrients that carry the virus and keep it active so that the sample can reach the laboratory for testing.

VTM virus preservation solution is suitable for the collection, preservation and transportation of common virus samples.

Such as new coronavirus, influenza virus, hand, foot and mouth virus. It can collect throat swabs, nasal swabs or tissue samples from specific parts.

And the stored samples can be used for subsequent nucleic acid extraction or Purification and other clinical experiments.

Virus Transport Medium (VTM) Definition

The virus transport medium sampling tube is a complete set of centrifuge tubes for sampling and transporting viruses.

What is Viral Transport Media

Such as new coronavirus, influenza virus, hand, foot and mouth virus in the microbial sampling transport tube.

Also known as the specimen transport tube. commoditized products.

A nutrient is used to carry and maintain the viability of test specimens (viruses) for the identification and further processing of samples in laboratories.

Viral Transmission Media Principles (VTM)

Since there is no cellular structure. Viruses cannot replicate themselves, but instead, invade genes into host cells and replicate new viruses with the help of the latter’s replication system.

After the virus sample is collected, in order to maintain the activity of the virus sample and prolong the survival time of the virus in the sample, the sampling swab will be put into the transportation medium for storage and transportation.

Transportation medium composition and inspection principle:

1. The combination of various antibiotics has broad-spectrum antibacterial and antifungal effects.

2. As a protein stabilizer, bovine serum albumin (BSA) can form a protective film on the protein shell of the virus, making it difficult to decompose and ensuring the integrity of the virus

3. The neutral environment constructed by Hank’s buffer helps to increase the survival time and infection stability of the virus.

4. Phenol red is an acid-base indicator, the discoloration area is 6.6 (yellow)-8.0 (red), and it is red at 7.2-7.4.

Vtm Kit

The most stable pH value of the viral outer layer protein is 7.2-7.4, which is also the pH value range of Hank’s buffer. If contaminating molds or bacteria are not inhibited by antibiotics, they can multiply, releasing carbon dioxide and lowering the pH.

At this time, the appearance of the sampling solution will change from red to yellow.

Notes:

To produce an isotonic solution containing proteins to protect viral structures, antibiotics to control microbial contamination, and one or more buffers to control pH.
Use the concept of assays such as nucleic acid amplification assays (NaAt) to prepare viral delivery media while preventing samples such as drying.

Composition of viral transport media (VTM Kits)

There are mainly the following parts:

1. Sampling swab with disposable sterile plastic rod/rayon head;

2. Sterile sampling tube containing 3ml of virus maintenance solution;

In addition, there are additional parts such as a tongue depressor, a biosafety bag, etc.

Viral delivery media typically have protective protein components, antimicrobial agents to prevent the potential for contamination, and buffers to maintain the pH of the solution.

Some VTMs also have additional ions and minerals that help keep such samples viable.

How to use Virus Transfer Media?

Liquid transfer media are primarily used to transfer the swab or material from the collection swab into the media.

It facilitates the collection and transport of samples in areas where it is not available.

Precautions for use:

1. Before sampling, indicate the sample information on the label of the cryopreservation tube;

2. According to different experimental purposes, use sampling swabs to sample at the corresponding parts (the most commonly used are nasopharyngeal swabs, pharyngeal swabs are the oral swabs in the product);

3. Immediately after sampling, put the swab into the sampling tube containing the virus transport medium, break it at the breaking point, and tighten the tube cover;

4. The specific sampling methods are as follows:

a) Nasal swab: Gently insert the flocked swab head into the nasal palate, stay for a while and then slowly turn to exit. Swab the other nostril with another swab, immerse the swab head in the sampling solution, break it at the breaking point, and discard it.

b) Pharyngeal swab: Wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with a flocked swab, and also immerse the swab head in the sampling solution and discard it.

Flocked Cotton Throat Oral swab 15Cm