Tag Archives: China swab manufacturer

Flocked Swabs |excellent absorption and elution ability

Flocked Swabs |excellent absorption and elution ability

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Flocked sampling swabs is a relatively new development trend of disposable sample collection equipment. As for flocked swab,their stable and reliable performance and excellent absorption and elution ability.

In order to improve the collection of samples, there are different types of flocking swabs. Such as pharyngeal swabs, gene detection swabs, forensic DNA detection swabs, gynecological cervical sampling swabs, microbial sampling swabs, etc. all flocked sampling swabs.

What is a nasopharyngeal flocked swabs?

The nasopharyngeal swab composed：a swab rod, a flocking swab cotton head.

Swab rod ：made of wood or PS or PP or ABS.

The sampling head of the swab shall be a flocked swab.

  •    To collect bacterial or virus samples from clinical pharynx, nasal cavity, ear canal or wound.
  •    Produced in 100000 level purification workshop and meet the requirements of ISO13485 quality system control and CE quality standard.
  •   This product can be EO or γ Irradiation sterilization.
  •   Single sterilization package and multiple sterilization packages are available.
Nasopharyngeal sampling_flocked swab

Nasopharyngeal sampling_flocked swab

How do you use a flocked swab?

Nasal swab collection method.

(1) Wet the swab in normal saline.

(2) Measure the distance from nostril to ear root with a swab and mark with your fingers.

(3) Insert the swab into the nostril in the direction perpendicular to the nose (face) until the finger touches the nose, keep the swab in the nose for 15-30 seconds, and then rotate it gently for 3 times.

(4) Put the swab into the virus preservation solution or transport medium, break the swab rod and place it completely in the tube.

(5) Tighten the pipe cover, mark it, put it in a plastic bag and seal it.

(6) If it is necessary to collect from two nostrils, one swab should be used respectively.

(7) Store at 4 ° C (short-term storage).

What is the reason for choosing flocked swabs?

The flocked swab provides the advantages of swab heads with different shapes.

Its rapid adsorption capacity and excellent release efficiency provide protection for limited or trace DNA.

It is especially suitable for the extraction of outdoor field and contact exfoliated cells.

The sampling swab is composed of nylon short fiber velveteen wool head and medical grade ABS plastic rod.

The function of nylon short fiber is like a soft brush, which can effectively improve the collection of cell materials;

The capillary movement between fibers forms a strong hydraulic pressure, so as to absorb the liquid sample;

At the same time, the sample is close to the swab surface and easy to elute.

The sampling swab of Dongguan Cell Medical company is a new type of medical sampling swab.

Innovative nylon fiber technology and high-tech spray planting technology.

It will not produce patient pain during collection.

Sterile Sampling Swab |Precautions for producing sterile sampling swabs

Sterile Sampling Swab |Precautions for producing sterile sampling swabs

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Sterile Sampling Swab |Precautions for producing sterile sampling swabs

Disposable sterile sampling medical swab, referred to as sterile swab.

Usually, sterile swabs have a collection material on the stem or at the top of the stem, which can be natural or synthetic. Such as polyester, flocking, etc.

The sterile swab material produced and sold by cell medical (Dongguan) Co., Ltd. is flocking.

The proprietary flocked process optimizes collection and elution, and the breakpoint forming handle can better help break, which is in line with ergonomic design.

Usually, the user holds the stem and wipes the collected material on the surface to collect the sample.

Swabs are sterile to maintain the integrity of the sample.

Usually, there is a resealable sterile container or bag to preserve the integrity during processing and transportation to the laboratory.

Including Covid-19 sampling, sterile workers, food producers and crime scene investigators.

For virus collect samples.

The material, size and shape of the sterile swab head may be different, such as round or pointed.

Some users need to collect products aseptically.

They collect samples from people, animals, even plants and other organisms, while others collect samples from inert surfaces, including furniture such as crime scenes and food processing tables.

Many times, The type of sample required determines the type of sterile swab selected by the user.

Sterile swabs may contain sterile water, which is the only acceptable water for laboratory studies.

Sterile swab manufacturers provide dry and wet swabs.

Usually, they pack sterile swabs in sterile packaging, such as paper, plastic or plastic coated paper covers.

Many manufacturers provide packages that are gamma irradiated and sealed to protect the integrity of swabs.

Some swabs are tubular and may contain liquids such as sterile water, sterile saline, or sodium chloride.

The user should check with the laboratory preparing to process the sample to know what type of swab or solution to use. For many applications, maintaining sample integrity is as important as using sterile swabs.

Many places use sterile swabs to monitor the environment or work area, control infection and its transmission, and monitor health management plans.

These include hospitals and medical institutions, food and drug processing facilities, and animal care facilities, such as veterinary clinics and zoos.

Materials for nasopharyngeal sampling swabs – manufacture

During the sampling process of the COVID-19, the pharyngeal swab is in direct contact with the sampling site, and the material of the sampling head is closely related to the subsequent detection.

The sampling swab head: made of flocked swab or artificial fiber.

Prohibited :calcium alginate sponge swab (including bamboo swab) .

Cotton products can not be used for the head of the swab, because the cotton fiber has strong adsorption on protein and is not easy to elute into the subsequent preservation solution;

The broken wooden stick or bamboo stick containing calcium alginate and wooden components will also adsorb protein and even inhibit the subsequent PCR reaction.

Recommended:PE fiber, polyester fiber, polypropylene fiber and other synthetic fibers  as the materials for manufacturing the swab head.

It is not recommended to use natural fibers such as cotton or nylon fibers, because nylon fibers (similar to toothbrush heads) have poor water absorption, which will lead to insufficient sampling and affect the initial inspection rate.

Pharyngeal swab material: prohibited! calcium alginate sponge.

viral transport medium tube

Viral Transport Medium Tube

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Viral Transport Medium Tube

Whether nucleic acid detection or virus detection, Viral Transport Medium Tube and Sampling Swab play a great role.

Sampling swab is used to collect virus samples, while virus preservation solution plays the role of preserving these virus samples.

Which not only ensures the integrity of nucleic acid virus, but also facilitates the later detection accuracy.

How many virus preservation solutions are there? What are the functions and differences?

At present, there are two kinds of virus preservation solutions on the market, namely inactivated virus preservation solution and non inactivated Viral Transport Medium.

Non-inactivated  Viral Transport Medium can protect the protein and nucleic acid of the virus.

Inactivated Viral Transport Medium usually contains the cleavage salt of inactivated virus to cleave the protein and protect the nucleic acid.

Specific uses of virus sampling tube:

1. The virus sampling tube is generally used for nucleic acid extraction and later virus isolation of influenza virus, hand, foot and mouth virus and rubella virus in clinical patients. The amount of liquid required is usually 35m or 5m. (with nucleic acid detection kit and spore culture medium)

2. For the collection and short-term transportation of avian influenza virus in the external environment. The amount of liquid required is usually 6ml.

3.  For daily monitoring and sampling of poultry, pigs and other animals. The amount of liquid required is usually 15m.

4. For short-term preservation of virus samples. (2-8 ℃ for 48 hours) for necessary cell culture.

5. Used for long-term preservation of virus samples (- 70 ℃ – 196 ℃ for one year) for necessary cell culture.

6. To collect clinical respiratory virus rapid detection kit.

7. To collect clinical samples of mycoplasma, chlamydia and Ureaplasma.

8. To transport tissue samples from the sampling site to the detection laboratory for necessary cell culture.

COVID-19 Swab Test

COVID-19 Swab Test |One Minute to Learn about Nucleic Acid Test

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COVID-19 Swab Test |One Minute to Learn about Nucleic Acid Test

So far, the number of confirmed cases of the global epidemic has exceeded 237 million, followed by the recurrence of the epidemic. We must do a good job in COVID-19 testing and strive to defeat the virus at an early date.

1. Why a nucleic acid test? What does it test for?

Nucleic acid swab detection is the direct evidence to find the existence of virus. The novel coronavirus pneumonia virus nucleic acid test positive, indicating that the presence of virus in the subjects, may be infectious.

If the patient has fever, respiratory symptoms or other clinical features of NCP, the patient can be diagnosed as NCP when combined with positive nucleic acid test; if there are no clinical symptoms, the nucleic acid test result is positive, can be judged as asymptomatic infection.

Novel coronavirus pneumonia is a diagnostic standard for new crown pneumonia.

2. Nasopharyngeal swab test and oropharyngeal swab test, which test method is better?

As we all know, nucleic acid detection generally adopts pharyngeal swab detection. Pharyngeal swab testing includes nasopharyngeal swab testing and oropharyngeal swab testing. The difference between the two lies in the different sampling positions, but there is no essential difference. Most medical institutions use oropharyngeal swab detection, because this detection method has less discomfort and is more convenient for sample collection.

3 .what are the precautions before and after COVID-19 Swab Test?

Before testing;

Get your ID card ready before you go out!

Wear masks correctly without fasting;

Try to avoid eating in the first 2 hours to avoid vomiting and other discomfort;

Try not to drink water, drink, smoke, drink or chew gum in the first 30 minutes;

When sampling, reduce swallowing action, swallow less saliva and don’t clear throat;

We should inform the testing personnel in advance whether they have a history of nasal surgery, bending of nasal septum, blood diseases, throat diseases or taking anticoagulants;

Before testing, remove the mask after the medical personnel give a signal, wear it immediately after sampling, and prepare a spare mask for easy replacement at any time after pollution.

Testing;

Consciously queue up and keep a distance of more than one meter;

When collecting oropharyngeal swabs, tilt your head back, relax as much as possible, breathe deeply, and open your mouth to make an “ah” sound, which is helpful to complete the sampling quickly;

The nose may be itchy during the collection of nasopharyngeal swabs. If you sneeze, cover your mouth and nose with paper towels or elbows immediately;

During the detection, try not to touch the detection platform and other articles, and do not touch the protective clothing of the collection personnel.

After detection

Leave the site immediately after sampling, and do not spit or throw garbage at the collection point and surrounding areas;

The inspectors shall pay attention to hand hygiene before and after the test. They can wipe their hands with hands-free disinfectant, hands-free alcohol, disinfection cotton, etc.

4 How can nucleic acid detection alleviate the discomfort of sampling?

Nasopharynx swab sampling and oropharyngeal swab sampling will make the sampled person feel slightly uncomfortable, which can be relieved after a short rest. Therefore, during the sampling process, the sampled person shall cooperate with the sampling personnel as much as possible, relax as much as possible, and do not over move the head to avoid scratching the mucosa with the sampling swab.

How to Obtain a Nasopharyngeal Swab Specimen

How to Obtain a Nasopharyngeal Swab Specimen

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How to Obtain a Nasopharyngeal Swab Specimen

As a non-invasive operation, nasopharyngeal swab sampling is the first choice for diagnosis, monitoring and differential diagnosis of New Coronavirus pneumonia. Its sampling randomness is high. However, the sampling personnel may have false-negative test results due to the different location, strength and time of scraping samples, which may have an adverse impact on the epidemic prevention and control. During the operation, the patient choked and splashed to produce an aerosol, which is very easy for medical staff to have occupational exposure.

This paper mainly explains in detail the protection of medical staff when collecting nasopharyngeal swabs, the collection, packaging and transportation of nasopharyngeal swabs, as well as the patient’s body position, operation methods, collection parts and precautions during specimen collection.

Effective communication and nurse-patient cooperation help the successful collection of nasopharyngeal swabs.

Before the operation, the degree of cooperation of patients should be effectively evaluated. Inform the patient of the collection purpose and precautions in advance.

Clinicians should be cautious if the patient has recent nasal trauma or surgery, a significant deviation of the nasal septum, or a history of chronic obstructive nasal patency or severe coagulation disease.

Standard protection is the foundation of successful nasopharyngeal swab collection

Operator preparation: first, wear protective clothing, wash hands with soap and water (or alcohol), and wear a pair of non-sterile gloves. Then, according to the recommendations of the Centers for Disease Control and prevention, wear an N95 or higher protective mask. Finally, wear a face mask to protect your face and eyes.

Swab Nasal Covid Sampling preparation: operating platform; Sampling materials, including nasopharyngeal test swab, VTM kits, inspection sheet and bar code, transport bag and hand disinfectant, to ensure the sterilization effect and validity period.

Obtain a Nasopharyngeal Flocked Swab Specimen

Obtain a Nasopharyngeal Flocked Swab Specimen

Nasal swab specimen collection method.

(1) Wet the flocked swab in normal saline.

(2) Measure the distance from nostril to ear root with a swab and mark with your fingers.

(3) Insert the swab into the nostril in the direction perpendicular to the nose (face) until the finger touches the nose, keep the swab in the nose for 15-30 seconds, and then rotate it gently for 3 times.

(4) Put the swab into the virus delivery medium, break the swab rod and place it completely in the tube.

(5) Tighten the tube cover, mark it, put it into the specimen bag and seal it.

(6) If it is necessary to collect from two nostrils, one swab should be used respectively.

(7) Store at 4 C or on ice (short term storage).

CDC recommends that all patients suspected of infection with COVID-19 use masks.

Ask the patient to take off the mask and wipe the nose with a paper towel to remove excessive secretions from the nasal cavity.

Remove the cotton swab from the package. Tilt the patient’s head back slightly so that the nasal tract is more accessible.

Let the patient close his eyes to reduce the slight discomfort caused by the operation.

Gently insert the swab into the nasopharynx along the nasal septum until resistance is felt.

Tips for nasopharyngeal swab sample collection.

The nasopharynx swab is inserted quickly. Do not hesitate. Some patients are intolerant to the operation of probing the nasopharyngeal swab into the nasal cavity, resulting in tears, foreign body feeling, etc. before the operation, ask the patients to inhale deeply in order to avoid choking and coughing.

When the cotton swab reaches the expected depth, do not continue to go deep, so as not to cause nasal mucosal damage and bleeding. During the stay, use finger strokes with the patient to read the seconds. It can distract attention, eliminate tension and motivate patients to ensure successful collection.

Never allow the patient to fully raise his head and bend the swab, which will lead to incorrect retention position of nasopharyngeal swab head and ultimately affect the accuracy of specimen collection results.

The operator can select the left / right side of the operator according to his left and right hands, and stand on the patient’s head and shoulder for operation. The patient’s head does not need to be biased to the opposite side. It is difficult to control the entry angle of the flocked swab when facing the side head, which may cause mucosal irritation. For patients with excessive tension, the operator can hold the patient’s hand and give comfort.

Quick questions and answers about nasopharyngeal swab sampling

1. Q: can carry out New Coronavirus test specimen collection personnel?

A: Qualified personnel with sampling skills after biosafety training.

2. Q: COVID-19 test specimen preferred?

A: Respiratory specimens.

Specific detection of

3. Q: infection in New Coronavirus includes?

A: Nucleic acid detection (nasal swab and pharyngeal swab); Virus isolation; Antibody detection.

4. Q: What are the key points of nasal swab collection?

A: Nasal swab: when the top of the swab reaches the posterior wall of the nasopharyngeal cavity, rotate it gently for one week.

5. Q: what respirator is used to collect COVID-19 respiratory specimens?

A: Masks N95 and above.

6. Q: how to disinfect hands after collecting samples?

A: After disinfection with disinfectant, take off the outer gloves, disinfect the inner gloves and wear new outer gloves.

7. Q: when taking off personal protective equipment, which of the following operations should be carried out first?

A: Spray disinfectant all over the body.

8. Q: What should we pay attention to when taking off conjoined protective clothing?

A: Ensure the contaminated surface of the conjoined protective clothing in the whole process, do not touch the inner clothing, and take off slowly and gently.

Contact us

Cell medical (Dongguan) Co., Ltd.
E-mail：flockedswabs@gmail.com
No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China.
Web：www.swabmanufacturer.com

viral transport medium with swab

Viral Transport Medium with Swab

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viral transport medium with swab | COVID-19 sampling and collection kits

The epidemic has disrupted the whole world and affected many industries.

During the outbreak of  COVID-19, people were paying close attention to the epidemic situation while doing personal protection. Novel coronavirus pneumonia and influenza were the most frequent period of communication in winter and spring. The most important way to control the epidemic is to select new patients with new crown pneumonia from many respiratory patients.

About viral transport medium with swab.

Swabs and virus transport media are a transport and collection system for long-term freezing, transport and collection of clinical specimens containing viruses.

The main species of organisms usually transported through this medium include chlamydia, urea plasma organisms and mycoplasma.

With the outbreak of COVID-19 pandemic, swabs and virus transport mediums were used to freeze and collect SARS-CoV-19 virus samples.

Rub a swab on the skin or vesicles to collect virus samples. The collected samples are then sent to the laboratory with transport media.

Why do swabs need virus transport media？

After virus collection, the traditional method can not be detected by PCR immediately. The virus itself will quickly dissolve in vitro and affect subsequent tests.

viral transport medium with swab -vtm kits

viral transport medium with swab -vtm kits

Purpose of virus transport media:

1. For monitoring and sampling infectious pathogenic microorganisms by disease control departments and clinical departments. It is applicable to the sampling of influenza virus (ordinary influenza, highly pathogenic avian influenza, influenza A H1N1 virus, etc.), hand, foot and mouth virus and other types of viruses.

2.For the sampling of mycoplasma, chlamydia and Ureaplasma.

3.. To transport nasopharyngeal swab samples or tissue samples at specific parts from the sampling site to the detection laboratory for PCR extraction and detection.

4.To preserve nasopharyngeal swab samples or tissue samples at specific sites for necessary cell culture.

Precautions for sample transportation.

1. Inspection time and temperature control:

After samples sampling, for inspection as soon as possible and sent to the laboratory within 2 ~ 4H as far as possible.

Transport at 2 ~ 8 ℃, and the transport time shall not exceed 72h. If it exceeds 72h, stored and transported at – 70 ℃ or lower.

2. Viral transport medium transport container:

Virus transport tube：waterproof, damage proof, leak proof, high temperature (low temperature) and high pressure resistant. 
Tube body: biological hazard signs, warnings and reminders with relevant regulations. 
Transport container: three-layer packaging system, namely inner packaging, middle packaging and outer packaging.

3. Transportation in the hospital:

The specimen delivery personnel shall be provided with secondary protection and carry 75% ethanol with them.

4. Long distance transportation:

The transport package of 2019 ncov specimens belongs to class A, and the corresponding UN number is un2814. The transporter shall wear safety protection according to the requirements of class II protection, and carry 75% ethanol with him. Drivers wear surgical masks or N95 masks and transport them by special vehicles. If transported by air, the packaging shall also comply with the pi602 classified packaging requirements of ICAO document Doc9284-AN / 905 technical rules for safe air transport of dangerous goods. At least one specimen transporter and driver shall transport specimens at the same time, and monitoring facilities for specimen transport process shall be equipped.

Contact us

  • Cell medical (Dongguan) Co., Ltd.
  • skype:+86-18814476009
  • E-mail：flockedswabs@gmail.com
  • No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China
  • Web：www.swabmanufacturer.com/

 

Flocked Swab Meaning

Flocked Swab Meaning

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Flocked Swab Meaning

This article will introduce what is the flocked swab, the purpose of the flocking swab, the application of the COVID-19 flocked swab and the material of the flocked swab, etc. The flocked swab manufacturer introduces. Give you a comprehensive understanding of flocking swabs.

what is the flocked swab?

Flocked Swab Meaning

Since the outbreak of COVID-19, the word “nasopharyngeal swab” has become a high-frequency word we talk about every day.

In recent months, the new crown pneumonia epidemic broke out again in countries all over the world. It’s worrying to watch the data released by WHO every day!!!

Flocked swab material

The flocked swab is made of nylon fiber flocking technology, and the front end is coated with nylon short fiber fixed vertically so that there is no absorption hole in the sampling area of the whole sampling swab, and the sampling sample will not be dispersed and retained in the fiber, which is conducive to faster and more efficient elution.

Traditional cotton swabs can not completely collect and elute samples while flocking swabs can elute 95% of samples. Therefore, the use of flocking swabs has great advantages for various sample collection applications.

The flocked swab is to deposit nylon fibres on the surface of the medical ABS rod in a certain direction through an electrostatic field to form a brush layer.

What is the purpose of flocked swabs?

Flocking swabs are the best choice for nucleic acid detection because they can better collect cell or biological samples at the collection site and release samples quickly.

Application: forensic medicine, DNA, microorganism, virus sampling, Oral swab, nasal swab etc.

What are flocked swabs used for in COVID-19?

The autumn and winter communication season is a period of the high incidence of common cold and influenza, and the most important means to control the epidemic situation is to select real patients with new crown pneumonia from a large number of respiratory tract patients.

At present, the most effective and widely used new crown sampling methods are nasopharyngeal swab and oropharyngeal swab sampling.

Flocked Swab Meaning _Application of flocking swab in COVID-19

Flocked Swab Meaning _Application of flocking swab in COVID-19

Nasopharyngeal swab collection method.

1. Keep the patient’s head still and remove the secretions on the middle surface of the anterior nostril;

2. Measure the distance from the nose tip to the earlobe with a swab and mark it with your fingers. Insert the swab into the nasal cavity in the direction perpendicular to the nose (face). When encountering resistance, it reaches the posterior nasopharynx, keeps the swab in the nose for 10 ~ 15 s, rotates it gently for 3 ~ 5 times, and take out the test flocked swab.

According to the American Society of Microbiology guidelines for the submission of clinical microbial specimens, the nasopharyngeal swab should be twisted on the nasopharyngeal mucosa, retained for 10-15 seconds, and then removed.

Feeling of nasal flocked swabs sampled by U.S. netizens.

netizens 01

netizens 01

It’s hard, and the tough guy still cries.

I have experienced both sampling methods.

It is not a painful cry, but a stress response caused by strong stimulation of the weak nasopharyngeal mucosa, with tears and tears.

netizens 02

netizens 02

No, as a girl, I don’t feel much about the nose swab sampling, except its excitement went to my head.

netizens 03

netizens 03

The more serious one is like eating mustard oil, and the lighter one is like blowing out your nose for several days.

Our netizens are so cute.

About oropharyngeal swab sampling.

oral swab sampling process_ Flocked Swab Meaning

oral swab sampling process_ Flocked Swab Meaning

Feeling of oral flocked swabs sampled by China's netizens.

Feeling of oral flocked swabs sampled by China’s netizens.

Pharyngeal(oral) swab: the collection process is faster (just a few seconds) and less painful, but you may feel nausea and vomiting, and some people have no obvious reaction.

As we are in the epidemic period, we should improve our awareness of self-protection when doing nucleic acid testing.

Flocked swab manufacturer introduces Cell medical (Dongguan) Co., Ltd.

Cell medical (Dongguan) Co., Ltd. is a comprehensive high-tech biotechnology enterprise.

We have invested a lot of scientific research strength to tackle key technical problems.

At present, our virus preservation tube and flocked swab have shown an irreplaceable role in the detection of new infectious diseases.

We support custom production, OEM and so on, and develop products with customers.

Welcome to leave a message for a consultation!

CONTACT US

Email:flockedswabs@gmail.com

What is a Nasopharyngeal Swab?

What is a Nasopharyngeal Swab?

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What is a Nasopharyngeal Swab?

COVID-19 Nucleic acid swab test  is a very effective and effective measure to prevent and control the new crown pneumonia epidemic.

The upper respiratory tract of human body includes nasal cavity, pharynx and throat.

That is to say, if people are infected with COVID-19, they can extract the nucleic acid of the virus in the nasal cavity, pharynx and larynx in 96 hours.

Therefore, we usually sample these locations in the rapid detection of new crown nucleic acids.

How to collect a nasopharyngeal swab?

Nasal swab sampling process

The first step is to collect secretions.

First, the medical staff will use a pharyngeal swab to wipe the subject’s nasal cavity or throat tonsils to collect salivary secretions.

nasal swab collection method

nasal swab collection method

The second step is to reserve samples.

After collecting the sample, immerse the nasopharyngeal swab in the preservation solution and screw on the tube cover to retain the sample.

The third step is to send samples for inspection.

Then, put the samples into clean sealed bags and send them to relevant parts for testing.

The fourth step is nucleic acid extraction.

Relevant departments will send the samples to the designated laboratory for nucleic acid extraction experiment.

Step 5: fluorescent PCR nasopharyngeal swab.

After collecting nasal samples, the experimenter uses fluorescent PCR to detect whether there is amplification reaction.

That is, to judge whether it is negative or positive.

Step 6:Finally, Receive test report.

Finally, according to the fluorescent PCR reaction results of nucleic acid detection, the nucleic acid detection vehicle inspection report is obtained, and I can query the results.

Sampling swab material

The sampling swab used for nasopharynx collection consists of a narrow rod made of a short plastic rod, one end of which is covered with an absorbent material. Such as cotton, polyester or flocked nylon.

The swab used for virus sampling should directly contact the sampling part.

The material of the sampling head is closely related to subsequent detection.

And the material requirements of virus sampling swab will be very high.

The virus sampling swab head shall be made of polyester (PE) synthetic fiber or artificial fiber.

Forbidden to use calcium alginate sponge or wooden swab (including bamboo stick).

However, calcium alginate and wood components can also absorb proteins after immersion in the preservation solution, and even inhibit the subsequent PCR reaction.

What are the advantages of using flocked sampling Nasopharyngeal swabs?

1. Flocked swabs provide better sampling.

Flocking swabs are a good choice for rapid diagnostic tests because they can better collect cells or organisms at the collection site and ensure the rapid release of the whole cell.

2. Enhanced diagnostic sensitivity.

Professional flocked technology turns each fiber into a velvet like swab, thus creating hundreds of thousands of contact points to better collect and release the collected samples.

Compared with non aggregated swabs, the increase in the number of target cells helps to improve the sensitivity of rapid diagnostic tests.

3. Rapid elution.

Their stable and reliable performance and excellent absorption and elution ability.

4. Easy handling and transportation.

sterilized and packaged separately, and the sterile swabs.

 

 

 

China Sterile Flocked Swab

China Sterile Flocked Swab Factory and Manufacturers

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China Sterile Flocked Swab GMP Factory and Manufacturers.

Cell medical (Dongguan) Co., Ltd. specializes in producing flocked swabs. Such as pharyngeal swabs, nasopharyngeal test swabs, oral swabs, nasal swabs etc.

Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.

Our GMP Factory-We support custom production, OEM

Our GMP Factory-We support custom production, OEM

·OEM / ODM: support

·Certificate: CE / FDA / ISO certification

·GMP factory: Yes

·Supply capacity: 500000-1000000 pieces / month

·Size: 150mm

Sterile Flocked Swab flocked swabs manufacturers

Sterile Flocked Swab flocked swabs manufacturers

The DNA extraction flocked swab produced by our company is made by nylon fiber flocked technology. The front end is coated with nylon short fiber fixed vertically. There is no absorption hole in the whole collection swab collection area, and the DNA cells will not disperse and remain in the fibers, which is conducive to faster and more efficient elution.

Independent sterilization packaging.

The benefits of flocked swabs for COVID-19 viral collection.

1. Sample collection process: The unique structure of the front end of the flocking swab can greatly increase the number of samples collected.

2. Sample dissolution process: The brush-like layer structure can release almost all collected samples. There are more advantages for the collection of trace DNA.

3. According to different analysis items, the corresponding soft or brush texture swabs have reached the maximum collection.

4. Disposable sterile sampling swab rod with breaking point.Learn more

5. Flocked swab technology improves the adsorption/release of samples and the sensitivity of the analysis.

6. The front flocked swab sampling is more precise and suitable for extracting more cells from the nasopharynx.

7. Packed in a transparent plastic tube to avoid biological pollution.

In short, the upright nylon fibre is like a soft brush, which can collect more cell samples. The capillary action between nylon fibers enhances the load of aqueous samples, and the samples are concentrated on the surface of the swab, which is easier to elute. Nylon flocking swab can absorb and release more samples.

Cell medical (Dongguan) Co., Ltd.  –China Sterile Flocked Swab Factory and Manufacturers.

Email:flockedswabs@gmail.com

 

 

COVID-19 Testing:PCR swab test,antibody test, and antigen test difference

COVID-19 Testing:PCR swab test,antibody test, and antigen test difference

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COVID-19 Testing :PCR swab test,antibody test, and antigen test difference.

PCR swab test（Nucleic acid test） and antibody / antigen detection are important means to confirm COVID-19, and also one of the important processes for patients to diagnose. But do you know what their detection principle is? What are the sample types? What is the inspection process and what are the problems? 

COVID-19 nucleic acid test

Nucleic acid detection has the characteristics of early diagnosis, high sensitivity and specificity. At present, real-time fluorescence quantitative RT-PCR is the most widely used technology. PCR swab testing is a very effective way to identify SARS-COV-2.

1. Principle of COVID-19 test kits?

The researchers used sampling swabs to collect samples of the virus, targeting the unique gene sequence of the virus.

By PCR amplification of the virus sample, the target DNA sequence we selected increased exponentially.

Researchers can combine the amplified DNA sequence with pre added fluorescent labeled probe fragments to generate fluorescent signals.

The more target genes amplified, the stronger the cumulative fluorescence signal.

In Virus-free samples, no target gene was amplified. Therefore, researchers can not detect enhanced fluorescence signals.

That is, we do not carry COVID-19.

Therefore, nucleic acid detection is actually to detect the accumulation of fluorescence signal to determine whether there is viral nucleic acid in the sample.

COVID-19 test kits_PCR swab test（Nucleic acid test）

COVID-19 test kits_PCR swab test（Nucleic acid test）

 

2. What are the methods of covid-19 testing samples?

Generally, PCR nasal swab test, pharyngeal swab, nasopharyngeal swab, sputum, bronchial lavage fluid, alveolar lavage fluid, etc.

Sampling swab consists of a nylon flocked swab.what is a flocked swab?

3. Steps of PCR swab test for COVID-19.

At present, pharyngeal swabs are the main sampling method for the diagnosis of novel coronavirus infection. Correct sampling is the key to improve the accuracy of nucleic acid samples collected from pharyngeal swabs. What are the factors affecting the accuracy of nucleic acid samples collected from pharyngeal swabs? There are three main aspects:

Depth of throat swab collection?

How long is the throat swab in contact with the mucosa?

Pharyngeal swab sampling “dot to dot” or wipe?

 

(1) Oral and pharyngeal swab collection tips

1. Sampling position: sit or semi-sit in decuffine position, with head slightly raised and mouth open, and head resting on the wall or against the back of the chair.

2. Standard sampling operation: Sampling personnel press the tongue of the subject with tongue depressors.

Pharyngeal swabs should be performed on both sides of the pharyngeal tonsil with “Quick wipe up and down” and on the posterior pharyngeal wall with “Quick left and right wipe” . Repeat 3 to 4 times.

Avoid contact with tongue, teeth and gums.

If you feel uncomfortable, quit, take a break, and try again.

Oropharyngeal Sampling flocked swab diagram

Oropharyngeal Sampling flocked swab diagram

3. Precautions: Drinking water before oral and pharyngeal swab test will dilute the virus attached to the pharynx, especially drinking hot water will inhibit the activity of the virus.

In order to improve the accuracy of pharyngeal swab nucleic acid detection, we recommend that subjects do not drink water 15-30 minutes before the detection, so as to ensure the high accuracy of pharyngeal swab nucleic acid test.

 

(2) Nasopharyngeal swab collection tips

1.The sampling personnel shall collect samples from the side of the subject, and the subject shall pull down the mask to expose only his nostrils. If there is sneezing reflex, the subject can cover it with elbow or paper towel.

nasopharyngeal sampling diagram

nasopharyngeal sampling diagram

2. Standard sampling operation: “one insertion, two stops and three rotations” — measure the distance from the tip of the nose to the front of the ear, half of the insertion length, usually about 4 cm for adults; Stay for 15-30 seconds to absorb nasopharyngeal secretions (depending on tolerance, minimum stay for at least 3 seconds; Rotate the nasopharyngeal swab for 1 week and slowly remove it.

The above is for the reference of sampling medical staff and patients, let’s work together to defeat COVID-19!

(3)Store samples

Dip the flocked swab head into the cell preservation solution(Virus transport medium), and tighten the tube cap immediately after breaking the tail;

Storage, put the sample tube into a sealed bag for timely inspection, and the inspection process requires a strict transportation environment, 2-8 ℃ storage.

(4) PCR TEST

Researchers operate nucleic acid extraction, extract inactivated virus samples for subsequent nucleic acid detection, and can use automatic equipment such as nucleic acid extractor.

Fluorescence PCR nucleic acid detection, that is, on the machine detection, the extraction of fluorescence PCR amplification reaction, needs 70-80 minutes.

process pcr test

process pcr test

4. What are the problems in nucleic acid testing?

False negative, due to improper sampling, improper specimen preservation, the use of different types of specimens and the use of different manufacturers of reagents may lead to nucleic acid test results “false negative” and missed diagnosis;

■ High requirements for detection equipment or platform, high sensitivity RT-PCR instrument is expensive, laboratory cleanliness and operator requirements are also high;

■ Nucleic acid testing is time-consuming. It usually takes 4-6 hours to complete an RT-PCR test.

Therefore, when the nucleic acid test is negative.

Relevant personnel can increase the detection of IgM and IgG antibodies to make up for the shortcoming that nucleic acid detection is easy to cause missed diagnosis.

 

COVID-19 Testing serum antibody test

Seven days after novel coronavirus pneumonia, the serum specific antibodies gradually emerged. The first is immunoglobulin IgM antibody, and then IgG antibody.

Thus, an elevated IgM antibody indicates a recent acute infection, and an elevated IgG antibody indicates a previous infection.

The biggest advantage of serological testing lies in its convenience and short detection time, which can effectively break through the limitations of existing detection technology on personnel and sites and shorten the detection time.

Novel coronavirus infection can be diagnosed in suspected cases if the serum specific IgM and IgG antibodies are positive, the IgG antibody changes from negative to positive, or the recovery period increases by 4 times or more than the acute period.

antibody test sampling process_COVID-19 Testing

antibody test sampling process_COVID-19 Testing

1. Principle of serum antibody test kit?

The sample is added to the sample adding hole, and the sample will move forward along the detection card under the action of chromatography.

If the sample contains new coronavirus IgM antibody, the antibody will combine with colloidal gold labeled virus antigen to form a sandwich complex, which will show a positive result.

If the sample contains New Coronavirus IgG antibody, the antibody will bind to colloidal gold labeled New Coronavirus antigen and form a sandwich complex, which will show positive results.

The detection card also contains a quality control line (line C) to judge whether the chromatography process is smooth.

2. What are the sample types for serum antibody detection?

Generally, it is blood, including serum, plasma and whole blood.

3.COVID-19 antibody test in three steps

1. Open the aluminum foil bag of the test card, take out the test card and place it horizontally on the table;

2. Use a pipette to absorb serum/plasma/whole blood sample and add it to the sampling site. Then use the same method to absorb buffer drops and add them to the sampling hole of the test card.

3. Wait 15 minutes and read the result.

4. What are the problems in serum antibody detection?

■ False positive, individual patients with rheumatoid factors in the blood, heterosinophilic antibodies, autoantibodies, drugs and tumor cells, etc., easy to cause cross-reaction test, so occasionally false positive results;

■ False negative, due to the existence of a certain window period of serum antibody detection methods and the sensitivity of the detection kit will appear false negative results.

Therefore, serum antibody test is only used as a supplementary test for suspected cases of novel Coronavirus nucleic acid negative test and cannot be used as a diagnostic indicator for screening alone.

The combined use of serum antibody detection and nucleic acid detection can help to improve the detection rate of the disease and find out the confirmed patients as much as possible, which is more conducive to the control of the epidemic.

Interpretation of results of nucleic acid combined with IgG and IgM antibody detection

COVID-19 Testing antigen detection

A novel coronavirus antigen test can novel coronavirus be detected directly in human body samples. The diagnosis is fast and accurate, with low requirements for equipment and personnel.

antigen test kit_SARS-CoV-2

antigen test kit_SARS-CoV-2

1. Principle of antigen detection kit?

SARS-CoV-2 , N, E and S protein antigens stimulate virus cells to produce specific antibodies.

2. What are the types of antigen test samples?

Usually samples from infected sites, e.g. oropharyngeal swabs, nasopharyngeal swabs, sputum, serum, plasma, etc.

 

3.COVID-19 antigen test in four steps

1. Drop the sample treatment liquid into the sample treatment tube;

2. Stir the sample swab and squeeze the tube wall until the specimen is fully eluted into the treatment solution;

3. Take out the detection card and add sample to the round hole;

4. Wait 15 minutes and read the result.

4. What are the problems in antigen detection?

■ False negative, higher sensitivity of antigen detection is required. Because New Coronavirus mainly affects alveolar and other lower respiratory tract. Therefore, sampling from the upper respiratory tract of nasopharynx and oropharynx cannot determine whether the pathogen can be detected. Or the sample contains a small number of viruses. All these factors may cause missed detection.

■ The preparation process is tedious and time-consuming. The development of the antigen kit requires the preparation of the recombinant antigen first, and then the preparation of monoclonal antibody in mice, which takes about two or three months. If the prepared antibody performance is not good, it needs to be prepared again, which takes another two or three months.

The joint detection

The COVID-19 Testing nucleic acid / antibody / antigen has different focuses and can not be substituted for each other. Multiple detection methods are combined and complement each other. Combine molecular biology and immune level detection to give play to their respective advantages and improve sensitivity and specificity. It can effectively shorten the detection window period, improve the positive detection rate, and provide double guarantee for all possible risk groups.

 

reference：Zhengtu Li，Yongxiang Yi et al. Development and clinical application of a rapid IgM‐IgG combined antibody test for SARS‐CoV‐2 infection diagnosis. J Med Virol.2020；1-7