Sterile Sampling Swab |Precautions for producing sterile sampling swabs

Sterile Sampling Swab |Precautions for producing sterile sampling swabs

Sterile Sampling Swab |Precautions for producing sterile sampling swabs

Disposable sterile sampling medical swab, referred to as sterile swab.

Usually, sterile swabs have a collection material on the stem or at the top of the stem, which can be natural or synthetic. Such as polyester, flocking, etc.

The sterile swab material produced and sold by cell medical (Dongguan) Co., Ltd. is flocking.

The proprietary flocked process optimizes collection and elution, and the breakpoint forming handle can better help break, which is in line with ergonomic design.

Usually, the user holds the stem and wipes the collected material on the surface to collect the sample.

Swabs are sterile to maintain the integrity of the sample.

Usually, there is a resealable sterile container or bag to preserve the integrity during processing and transportation to the laboratory.

Including Covid-19 sampling, sterile workers, food producers and crime scene investigators.

For virus collect samples.

The material, size and shape of the sterile swab head may be different, such as round or pointed.

Some users need to collect products aseptically.

They collect samples from people, animals, even plants and other organisms, while others collect samples from inert surfaces, including furniture such as crime scenes and food processing tables.

Many times, The type of sample required determines the type of sterile swab selected by the user.

Sterile swabs may contain sterile water, which is the only acceptable water for laboratory studies.

Sterile swab manufacturers provide dry and wet swabs.

Usually, they pack sterile swabs in sterile packaging, such as paper, plastic or plastic coated paper covers.

Many manufacturers provide packages that are gamma irradiated and sealed to protect the integrity of swabs.

Some swabs are tubular and may contain liquids such as sterile water, sterile saline, or sodium chloride.

The user should check with the laboratory preparing to process the sample to know what type of swab or solution to use. For many applications, maintaining sample integrity is as important as using sterile swabs.

Many places use sterile swabs to monitor the environment or work area, control infection and its transmission, and monitor health management plans.

These include hospitals and medical institutions, food and drug processing facilities, and animal care facilities, such as veterinary clinics and zoos.

Materials for nasopharyngeal sampling swabs – manufacture

During the sampling process of the COVID-19, the pharyngeal swab is in direct contact with the sampling site, and the material of the sampling head is closely related to the subsequent detection.

The sampling swab head: made of flocked swab or artificial fiber.

Prohibited :calcium alginate sponge swab (including bamboo swab) .

Cotton products can not be used for the head of the swab, because the cotton fiber has strong adsorption on protein and is not easy to elute into the subsequent preservation solution;

The broken wooden stick or bamboo stick containing calcium alginate and wooden components will also adsorb protein and even inhibit the subsequent PCR reaction.

Recommended:PE fiber, polyester fiber, polypropylene fiber and other synthetic fibers  as the materials for manufacturing the swab head.

It is not recommended to use natural fibers such as cotton or nylon fibers, because nylon fibers (similar to toothbrush heads) have poor water absorption, which will lead to insufficient sampling and affect the initial inspection rate.

Pharyngeal swab material: prohibited! calcium alginate sponge.

Saliva Sample Collection Kit :Disposable saliva DNA RNA sampling tube

Saliva Sample Collection Kit :Disposable saliva DNA RNA sampling tube

Saliva Sample Collection Kit :Disposable saliva DNA RNA sampling tube.

Non-invasive ! No blood, No needle.

No risk of infection, no need accompany of Doctor, no need refrigerate.

people can collection their Saliva sample at home and mail to Lab for DNA test.

what are the differences between the nasal swab and saliva tests for covid 19?

What is saliva collection kit?

In recent years, with the development of medicine, the examination technology has become more and more high-end. And the examination items have become more and more accurate.

Saliva is a complex mixture, which not only contains various proteins. It also contains DNA, RNA, fatty acids and various microorganisms. Therefore, it is possible to diagnose diseases by detecting saliva. Gene detection and analysis.

Saliva collector plays an important role in the process of collecting, transporting and storing saliva samples.

It is noninvasive and easy to self-management, and reduces the risk of medical staff exposure.

Saliva Collection Kit suitable for SARS-CoV-2 sample collection.

Nucleic acid detection is the most widely used new crown detection method.

Including PCR nasopharynx swab, oropharyngeal swab  and saliva sample test.

High precision. As long as the collection, breeding and separation of nucleic acids are successful, there is basically no false positive or false negative.

But the sampling staff are easy to be exposed and the risk is high; With one more step of extracting nucleic acid, unskilled technicians are likely to fail to extract, resulting in false negative, or pollute with other people's samples, resulting in false positive.

 

How to use saliva sample collection kit

saliva process 01

 

1.Before collecting saliva,relax your cheeks andgently massage cheeks with fingers for 15~30 seconds to produce saliva. Gently

2mL  saliva into thefunnel until the liquid saliva(non-bubble)reaches the height of 2.0 ml scale line. Saliva samples collected shall be free of impurities and sputum.Don't spit all over the vial.

saliva process 02

 

2.Hold the Hold the storage vial in hand and keep it upright, then insert the bottom of storage vial into the saliva collection cup, and screw down. Then the storage liquid in the vial will flow into the saliva collection vial.

Saliva Sample Collection sampling process 03

 

3.Keep the collecting vial upright, unscrew the funnel, take out the clean saliva collecting vial cap from the packing box,screw and tightenit on the saliva collecting vial. The vial caps have a chok-ing hazard. Keep out of reach of children.

Saliva  process 03

 

4.Shaking Turn the collecting vial upside down for 10 times to fully mix saliva ten times and preservation solution.

Saliva  process 05

 

5.Take out a bar code from the packing box and stick it on the saliva collecting vial, then put the collecting vial with bar code and two bar codes into the sample bag for storage, transportion or testing, and the remaining bar code is kept by the user for subsequent information feedback.

Comparison of advantages of various testing methods of COVID-19

Comparison of advantages of various testing methods of COVID-19

advantages of various testing methods

The detection methods of COVID-19 mainly include nucleic acid detection, antibody detection and antigen detection. Due to the low detection rate of antigen, the SARS-CoV-2 detection mainly focuses on antibody and nucleic acid detection. Nucleic acid detection has the characteristics of early diagnosis, high sensitivity and specificity; However, antibody detection is convenient and rapid, which can be used as a supplementary means of nucleic acid diagnosis. Due to the limitations of “false positive” and “false negative” antibody detection, it is not suitable for general population screening such as returning to work,  and school, nor for epidemiological investigation in low epidemic areas.

NAT Detection process

The detection procedure needs to go through five steps: sampling, sample retention, preservation, nucleic acid extraction and computer detection.

First, collect samples according to the instructions of the kit. The sample types include pharyngeal swab, nasal swab, sputum, bronchial lavage fluid, alveolar lavage fluid, etc.

Because RNA is easy to degrade, flocked swabs without RNase and storage tubes without RNase are used when collecting samples.

After obtaining the patient’s sample, it needs to be tested as soon as possible. If it cannot be tested immediately, it needs to be packaged at low temperature and sent to a special testing institution for testing.

After receiving the sample, the testing institution shall extract the nucleic acid from the sample. The nucleic acid extraction reagent shall use the nucleic acid extraction kit specified in the approved product manual.

Finally, fluorescence PCR nucleic acid detection, that is, machine detection. It takes 70-80 minutes to amplify the extract by fluorescence PCR.

Sample collection, oropharyngeal swab or nasopharyngeal swab

Sample collection, oropharyngeal swab or nasopharyngeal swab

NAT Detection process Nucleic Acid Testing

NAT Detection process Nucleic Acid Testing

Antibody detection

After the virus invades the human body, the human body will produce corresponding specific antibodies for defense.

According to different molecular structures and antigen specificity, scientists divide immunoglobulins into five categories: IgG, IgA, IgM, IgD and IgE.

After the antigen enters the human body for the first time, plasma cells secrete antibodies after a certain incubation period. IgM first appeared, but the antibody had short maintenance time, low concentration and low affinity. It lasted for several days to several weeks in the blood. It was a diagnostic index of acute infection; When IgM is about to disappear, the content of IgG reaches the peak. IgG appeared late, but its concentration was high, its maintenance time was long and its affinity was high. The positive serum IgG indicates the middle and late stage of infection or previous infection.  But the antibody content increases rapidly in the short term, which may be several times to dozens of times higher than the original antibody content. It is mainly IgG, which lasts a long time in the body, and IgM rarely increases.

In this epidemic, the researchers studied patients with covid-19 and found that after the virus invades the human body, IgM antibody takes about 5-7 days to produce and IgG antibody takes 10-15 days to produce.

Antibody detection kits-advantages of various testing methods

Antibody detection kits-advantages of various testing methods

Comparison of advantages and disadvantages of two different detection methods

Compared with the serological detection of antibodies, Nucleic Acid Testing can detect patients in the window stage.

Early detection of infected persons is the “gold standard” for COVID-19 detection, but it requires higher detection equipment or platform.

The high sensitivity RT-PCR instrument is expensive, and the laboratory cleanliness and operator requirements are higher. In addition, nucleic acid detection takes a long time. Considering the sample transportation and sample backlog, the results can be reported as soon as 24 hours.

Compared with nucleic acid detection, blood samples detected by antibody serology are easier to obtain. If the RNA of the virus detected by nucleic acid is the direct evidence of the existence of the virus, the antibody detection is the antibody stimulated in the patient’s blood, which is indirect evidence and has a suggestive effect on the clinic.

When nucleic acid detection is negative, adding IgM and IgG antibody detection can make up for the shortcoming that nucleic acid detection is easy to cause missed diagnosis.

About us

Cell medical (Dongguan) Co., Ltd., a professional VTM kits and swabs manufacturer, has many years of experience in medical application molding and flocked treatment heads and swabs.

Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.Certificate:FDA,CE,EU,Free Sale,Export license based on (GMP).

We produce flocked swabs, VTM kits, Virus transport medium, with multiple automatic production lines.We support custom production, OEM and so on, and develop products with customers.

  • E-mail:flockedswabs@gmail.com

     

    Nasal mini-tip flocked swab

    Nasal mini-tip flocked swab

    Nasal mini-tip flocked swab Medical Nasopharyngeal swab

    Nasopharyngeal swabs are a means of detection. Doctors use nasopharyngeal swabs mainly for special bacterial group infection or virus infection in the nasopharynx. They will use sterile pharyngeal swabs to enter the nasopharynx and throat through the nasal cavity and wipe the mucosa. Conduct bacterial culture, virus culture and drug sensitivity test to determine our treatment plan and diagnosis. This examination is a non-invasive examination.

    Product description

    medical nasal nasopharyngeal mini tip flocked swab

    1. EO sterile, individual packing
    2. With molded breakpoint
    3. Ergonomic and anatomic design, easy to use
    4. Perpendicular nylon fibers, increased assay sensitivity

    CM-96000 NASAL SWAB

    CM-96000 NASAL SWAB

    Product Flocking swab for nasal
    Item No CM-96000
    Material Tip: 100% nylon, handle: plastic (ABS)
    Usage Single use for Nasopharyngeal excretion collection
    Size Diameter: 3mm

    Length: 150mm
    Breaking point: 80mm

    Packing 1pc/pouch, 10000pcs/ctn
    sterile, individual packing cm-96000 Packaging picture

    sterile, individual packing cm-96000 Packaging picture

    Nasal mini-tip flocked swab Packaging & Delivery

    Packaging Details
    Individual Sterile Package
    100 pcs/zip pouch
    5000pcs /carton box
    Carton Size: 52*40*30 cm
    G./W.: 6.5kg
    Port
    shenzhen

    Contact us

    • Cell medical (Dongguan) Co., Ltd.
    • E-mail:flockedswabs@gmail.com
    • No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China
    • Web:www.swabmanufacturer.com/
    Nasal swab for flu

    Nasal swab for flu,Rapid antigen nasal or throat swab

    Nasal swab for flu_Rapid antigen (nasal or throat swab) test

    Influenza is common in winter and spring. The clinical manifestations are mainly systemic poisoning symptoms such as high fever, fatigue, headache, cough and systemic muscle pain, while the respiratory symptoms are mild.

    Influenza viruses are prone to mutation, infectious, susceptible to population and high incidence rate. They have caused many outbreaks in the world, and are important public health issues worldwide.

    Why should we detect and prevent influenza?

    According to the World Health Organization (WHO), seasonal influenza can cause 3-5 million severe cases and 290000-650000 deaths worldwide every year.

    The researchers believe that children under the age of 5 are at high risk of severe influenza, but children under the age of 2 are at the highest risk, and infants under 6 months have the highest hospitalization rate and mortality.

    Pregnant women, infants, the elderly, patients with chronic basic diseases and other high-risk groups have a higher risk of serious diseases and death after suffering from influenza.

    Nasal swab for flu : common influenza test method

    Nasopharyngeal sample collection

    Nasopharyngeal sample collection

    Influenza is an acute inflammation of the respiratory tract caused by the patient’s infection with influenza virus. The patient can have a series of symptoms such as cough, expectoration, fever, sore throat, stuffy nose, runny nose and general fatigue. In case of influenza, we should conduct relevant examinations in time to further clarify the diagnosis, including the following tests:

    First, blood routine test, patients with influenza can promote the increase of lymphocytes. If combined with bacterial infection, there can be the increase of leukocytes and so on.

    Second, scientists can culture throat swabs of influenza viruses. In throat swab culture, researchers can detect the related virus.

    Third, influenza patients can do second-generation sequencing, including sputum second-generation sequencing, which can determine the type of virus infected.

    Fourth, influenza patients can do the detection of respiratory pathogens to further clarify the pathogens of infection.

    At present, the two most common influenza tests are rapid antigen diagnostic test  and influenza virus cell culture test. Both tests rely on Nasopharyngeal swabs, pharyngeal swabs, etc. for virus sampling. The most commonly used sampling swab is nylon flocking swab.

    Nasal swab for flu:Application

    It is suitable for the detection and sampling of COVID-19, influenza virus , hand foot mouth disease virus, COVID-19, measles rubella and other types of viruses.

    About nasal swab sampling

    Why use flocked nylon swabs?

    flocked swabs_Cell medical (Dongguan) Co., Ltd.

    flocked swabs_Cell medical (Dongguan) Co., Ltd.

    Flocked nylon swab: excellent sample collection / release ability, release rate > 90%.

    Conventional fiber swab: the sample is intercepted by the gap in the fiber mass. That is, the sample is penetrated, diluted and intercepted in the fiber.  Acquisition is slow and release is slower.

    The sampling time of flocked swab is 3-5 seconds, while that of traditional swab is 15-120 seconds or even longer.

    This caused pressure on the staff during the diagnosis process. Only by extracting samples faster can we complete the collection and analysis more efficiently.

    Precautions for patients with nasopharyngeal swab:

    ① Please do not take antibiotics a few days before making nasal swabs;

    ② Do not use disinfectant mouthwash or smear the focus a few hours before nasal swab.

    ③ Do not eat, smoke or drink a few hours before doing nose swab.

    Precautions for Sampler:

    ① . the sampler shall stand on the side of the patient for sampling.

    ② The patient is required to pull down the mask to expose only his nostrils. In case of sneezing reflex, the patient can cover it with elbows or paper towels.

    If the sampler is not directly in front of the patient, the exposure risk will be lower.

    ③ If the patient’s turbinate is hypertrophic, the nasal tract is narrow, and the insertion of the nasal swab feels resistance. Try to change one side of the nasal cavity or directly change to oropharyngeal swab collection.

    ④ Those with nasal allergy symptoms are easy to induce sneezing. It is recommended to take oropharyngeal swab samples.

    ⑤ Remember to wear a protective mask / screen. For individuals who are highly suspected or basically diagnosed, or who have severe cough and hiccup, they should put on protective clothing.

    ⑥ After sampling, replace gloves, disinfect hands, and spray disinfectant where patients have touched.

    Finally, we should pay attention to nasal swab for flu in special times. Remember to wear masks and personal hygiene when going out. Once you find the virus, please isolate it to avoid re transmission.

     

    Nasal swab pcr test

    Nasal swab pcr test

    Nasal swab pcr test COVID-19 Antigen Rapid Test Kit

    Detection of COVID-19 by nasal swab PCR is an accurate and reliable method. Positive detection means you may have SARS-CoV-2. Negative detection means you may not have SARS-CoV-2 when testing.

    In order to ensure your safety, if you have symptoms of COVID-19. Or people who have been exposed to the virus and tested positive should be tested.

    What is the symptom of COVID-19?

    If you have the following symptoms,if you have the following symptoms, please buy covid-19 antigen rapid test kit immediately or go to a nearby hospital for PCR test.

    1.Fever;

    2.General fatigue;

    3.cough;

    4.Dyspnea or even respiratory failure;

    5.Nausea;

    6.vomiting;

    7.abdominal pain;

    8.diarrhea;

    Symptoms of New Coronavirus pneumonia are not necessarily typical, and some patients may not have clinical manifestations at the early stage of New Coronavirus infection. Then clinical manifestations will appear, that is, “asymptomatic infection” in incubation period.

    In other words, try to avoid activities in places with crowd gathering, closed space and poor ventilation.

    What is nasal swab PCR test?

    Polymerase chain reaction (PCR) uses a piece of DNA as a template. Then, with the participation of DNA polymerase and nucleotide substrate, the DNA was amplified to a sufficient number for structural and functional analysis.

    Scientists use PCR technology to amplify a small amount of RNA in the sample into deoxyribonucleic acid (DNA) and replicate until COVID-19(if present) is detected.

    What should we pay attention to before doing nucleic acid test?

    1.Try not to eat 2 hours before sampling to avoid vomiting.

    2. Do not smoke, drink, drink water or chew gum 30 minutes before sampling.

    3. The tester needs to wear a mask correctly and remove the mask before testing;

    4. Wear it immediately after testing, and prepare a spare mask for easy replacement at any time after pollution.

    5.When you feel sour and itchy in your nose or sneeze, you can immediately cover it with a paper towel or elbow.

    Before collecting nasopharyngeal swabs and before being tested, the collection personnel shall be informed whether they have relevant past medical history. For example, history of nasal surgery, history of deviation of nasal septum, blood diseases, throat diseases, etc

    How long will we know the pcr test results?

    The test results of sampling in the morning are generally available from 18:00 to 19:00.

    The detection results of afternoon sampling can generally be queried around 22:00.

    What’s the difference between the PCR and antigen tests for COVID-19?

    Polymerase chain reaction (PCR): PCR is the most reliable and accurate method to detect active infection. PCR tests usually give results in a few hours.

    Antigen detection: detect protein fragments on the surface of covid-19. Antigen detection usually takes only 15 to 30 minutes.

    How can we prevent COVID-19?

    Firstly, keep good personal hygiene habits, wear masks when going out, wash hands frequently, clean the room, and wipe the floor and other furniture with disinfectant. Second, disinfect your hands with 75% alcohol every time you go out and go home, reduce the number of times you go out, and wear qualified masks. And the diet should also be light, do a good job of physical exercise and enhance personal physique.

    Most importantly, if our conditions permit, we should vaccinate and conduct multiple nucleic acid tests.

    If our Nasal swab pcr test results are positive,what should we do?

    If our test results are positive, don’t be afraid. We should wear masks and go to the nearby Hospital for follow-up in time.

    Repeat the nucleic acid test.Don’t be afraid and hide the test results. Timely medical treatment is the best choice.

    Cell medical (Dongguan) Co., Ltd. is a comprehensive high-tech biotechnology company and an OEM factory of flocking swabs in China.We support custom production,our factory produces flocked swabs, pharyngeal swabs, nasal swabs, oral swabs,Rapid Antigen Test Kit, virus sampling tubes, transportation media, sample preservation solution and nucleic acid extraction kit.

    Welcome to leave a message and consult us!

     

     

    What is a sterile swab

    What is a sterile swab?

    Sterile swab is the full name of disposable sterile sampling swab. The sterile sampling swab does not contain or other live microorganisms. Common sterilization methods include autoclave, EOS (ethylene oxide gas) and γ-ray irradiation. In medicine, we usually use sterile swabs for biological sample collection.  Such as bacterial sample processing, COVID-19, nasal swab sampling, throat swab sampling, oral swab sampling, virological cell culture and so on are ideal ways.

    Swabs are sterile to maintain the integrity of the sample. Usually, there is a resealable sterile container or bag to preserve the integrity during processing and transportation to the laboratory. Many different people use sterile cotton swabs, including medical personnel, food manufacturers and crime scene investigators.

    Sterile swabs can be used to collect samples. The material, size and shape of the sterile swab head may be different, such as round or pointed. Traditional swab may be made of cotton fiber, polyester or calcium alginate.  Collection and publishing volumes are large and may be corrupted. Traditional swab laboratory staff also need to use scissors to cut, which is too troublesome and time-consuming.Our new flocked disposable sampling cotton swab greatly reduces the time cost and has the advantage of being easier to use than traditional swabs.

    The main advantages of our sterile flocked swab are as follows.

    1. It has extraordinary water absorption capacity and can increase the number of samples collected on the watch from 20% of the traditional sampling swab to 60%.

    2. The release rate of collected samples exceeds 90%, which ensures the high reliability of the results.

    3. Select different preservation solutions for different types of samples.

    4. The plastic rod has a unique fragile design, which is convenient for sample transportation.

    5. Ethylene oxide sterilization, irradiation sterilization and independent packaging.

    Sampling operation method.

    1.Nasopharyngeal swab sampling.

    Nasopharyngeal Sampling diagram

    Nasopharyngeal Sampling diagram

    ①Insert a sterile swab into the nostril of the patient,reaching the surface of the posterior nasopharynx.

    ②Swab over the surface of the posterior nasopharynx. Hold the swab in the nosefor15-30s and rotate it 3 times.

    ③Withdraw the sterile swab from the nasal cavity.

    ④Put virus sampling tube vertically.

    ⑤At the breaking point, disconnect the swab, discard the tail, and completely place the swab into the tube.

    ⑥Tighten the tube cap, mark it, put it into the biosafety bag and seal it.

    2.Oropharyngeal swab sampling

    Oropharyngeal Sampling diagram

    Oropharyngeal Sampling diagram

    ① Keep the head slightly tilted, the mouth open, and the pharyngeal tonsils on both sides exposed.

    ②The sampling personnel shall wipe the root of the tongue with a swab. Wipe the pharyngeal tonsils on both sides of the subject back and forth with a strong force for at least 3 times, and then wipe the posterior pharyngeal wall up and down for at least 3 times.

    ③Put the sampled swab into the virus sampling tube, break the swab rod at the mouth of the tube cover and completely put it into the tube.

    ④Tighten the tube cap, mark it, put it into the biosafety bag and seal it.

    ⑤Submit for inspection in time.

    Anal swab sampling

    Anal Sampling diagram

    Anal Sampling diagram

    ①The patient is in the knee chest position or lateral position.

    ② Gently insert a sterile cotton swab into the anus for 3cm-5cm, and then gently rotate and pull it out.

    ③Immediately put it into a 15ml outer screw cap sampling tube with 3ml-5ml virus preservation solution.

    ④Fold off the tail of the cotton swab, tighten the sealing cover, and then submit it for inspection in time.

    ⑤In order to avoid embarrassment of the examinee, anal swab sampling is generally conducted in a more private place, and the operation requirements of anal swab sampling are more strict. Generally, there will be no obvious discomfort after anal swab sampling.

     

    New Coronavirus nucleic acid detection

    New Coronavirus nucleic acid detection steps

    New Coronavirus nucleic acid detection steps

    1、 The specific operations are as follows

    1. Virus sampling:

    Take the patient’s saliva sample or nasopharyngeal flocked swab sample, and save the virus sampling tube.Virus preservation solutions commonly used in disposable virus tubes include inactivated and non inactivated types. The following are two models of virus transport medium:

    1) Inactivated type: the inactivated virus transport medium characterized by killing the virus and retaining nucleic acid fragments. Application:detect COVID-19, influenza virus, hfmv and influenza A.

    2) Non inactivated type: the non inactivated virus transport medium does not contain lysate. It can retain the activity and integrity of pathogens.Application:virus culture and reproduction to make the detection results more accurate.

    flocked swab specimen collection_New Coronavirus nucleic acid detection steps

    flocked swab specimen collection_New Coronavirus nucleic acid detection steps

    2. Nucleic acid extraction:

    Extract the genetic material from the patient’s sleeping fluid or nasopharyngeal swab sample. If a patient carries a virus, there will be RNA of the virus’s genetic material in the sample. Sampling personnel should pay attention to prevent RNA degradation when extracting RNA. At the same time, the extracted DNA shall be stored at – 20 ℃, and the RNA and DNA requiring long-term storage shall be stored at – 70 ℃ or liquid nitrogen.

    3. Reverse transcription synthesis of cDNA:

    reverse transcription of RNA in the extract, reverse transcription of RNA into cDNA, reverse transcription of cDNA synthesis reaction, using reverse transcription primers, dNTPs, reverse transcriptase, RNase inhibitor and DTT. Buffer, an appropriate amount of ultrapure water without RNA / DNase and RNA template. We propose to use commercially available RT-PCR one-step reagent for one round amplification of di.

    4. PCR amplification reaction (nested PCR amplification method with secondary amplification):

    Use specific primers of virus cDNA for PCR amplification in an amplification instrument. Nested PCR amplification using secondary amplification.

    5. Result analysis and judgment:

    If the DNA band of the virus is amplified, it is determined that the virus exists in the patient; If no DNA band is amplified, it is determined that the sample taken by the disease has no virus.

    2、 Experimental precautions:

    ① We should set up two positive controls and two negative controls for each test. Only when the positive control enlarges the expected fragment, the negative control does not enlarge any fragment, and the results of two parallel samples are consistent.We can determine the nucleic acid positive or negative reaction results.

    ② Nucleic acid test is positive: if nucleic acid is positive, we should repeatedly collect samples for retest. If the retest result is nucleic acid positive, we can confirm that the sample result is nucleic acid positive. If the retest result is nucleic acid negative, the nucleic acid test result is uncertain.

    Facing COVID-19 variation strain

    Facing COVID-19 variation strain

    Facing COVID-19 variation strain. How should we deal with it?

    What are the variant strains of COVID-19?

    The variation of COVID-19 B.1.621 was first confirmed in Columbia in January this year. The World Health Organization named it Miao strain and recently listed it as a mutant strain that needs attention. Its influence level is lower than that of the delta strain. Preliminary data show that the Miao strain is similar to the beta strain and will weaken the effectiveness of the antibody.

    At present, there are four variants defined by who as “needing attention”, including alpha, beta, gamma and delta. And five variants “needing attention”, including ETA, iota, kappa, lambda and mu.

    How should we deal with it?

    Weightier than Mount Tai, I think the most important thing about this new crown epidemic is: life is above everything else and responsibility is heavier than Mount Tai.

    We often ask: where is the value of life? After this epidemic, we answer is: responsibility.

    A person and an enterprise must have a sense of responsibility before their life can be valuable. If a person is not responsible and has no sense of responsibility, his life is worthless.

    First, Let’s do a good job in personal protection. Don’t worry too much.

    As the general public, we can prepare some masks, medical goggles,Rapid Antigen Test Kit,New Coronavirus test kits and so on.We advocate new crown nucleic acid testing at home

    Rapid Antigen Test Kit specimen testing

    Rapid Antigen Test Kit specimen testing

    Cell medical (Dongguan) Co., Ltd. is a comprehensive high-tech biotechnology company and an OEM factory of flocking swabs in China.We support custom production,our factory produces flocked swabs, pharyngeal swabs, nasal swabs, oral swabs,Rapid Antigen Test Kit, virus sampling tubes, transportation media, sample preservation solution and nucleic acid extraction kit. Our GMP factory was built in 2016,the factory has ISO13485 quality management system, CE, FDA and other qualifications.

    Secondly, we should keep regular work and rest and keep learning.

    Adhere to exercise and treat your body: regular work and rest, balanced diet, exercise and regular physical examination. After all, the body is the capital of revolution, and immunity is the first competitiveness.

    Finally, if your physical condition permits, please vaccinate as soon as possible. For you, for your family and for our national peace and security.

    In order to better prevent COVID-19 variation strain, Cell medical (Dongguan) Co.,Ltd. increases production and guarantees supply.

    COVID-19 Saliva Collection Kit

    COVID-19 Saliva Collection Kit Instructions

    COVID-19 Saliva Collection Kit Instructions

    No needles, no blood, no accompany of Doctor, no need cooling,people can self-collect their Saliva RNA sample at home and mail to Lab for PCR test.Saliva Collection kits.

    What is the saliva collection kit used for?

    Used for sampling, transporting and storing saliva samples.

    Saliva collection kit consists of a saliva funnel (with collection tube) and a test tube with 2ml cell preservation solution. It is used to collect salivary DNA / RNA samples for virus PCR analysis. The average yield of RNA from 2ml saliva sample was 110 μ g. But the output may be 15 μ G to 300 μ G.

    DNA / RNA was then isolated from human saliva samples for nucleic acid detection. Including the detection of COVID-19.

    The biggest advantage of the saliva collection kit is that it is convenient and direct. Even the elderly and children can easily collect saliva into the test tube. Collecting saliva DNA / RNA samples allows direct viral PCR analysis without additional steps to process saliva collected using the saliva collection kit.

    The saliva collection kit is very suitable for home and commercial use.

     

    How to use saliva collector?

    Clean the mouth:30 minutes before collecting a saliva sample, please wash away the debris in the oral cavity with absorption.

    1: install the collecting funnel on the collecting pipe and spit saliva into the collecting funnel until the saliva reaches the filling line of the collecting pipe.

    2: hold the collecting tube vertically with one finger and close the funnel cover with one hand.

    3:Open the DNA preservation solution tube and unscrew the tube capand pour the DNA preservation solution into the salivary collector.

    4: hold the collection tube vertically and rotate it gently to separate it from the collection funnel.

    COVID-19 Saliva Collection Kit  Instructions

    COVID-19 Saliva Collection Kit Instructions

    5: after screwing on the cover of the acquisition tube, gently shake it for 5 seconds for detection or storage at room temperature.

    Saliva collector structure

    Characteristics of saliva collector

    Simple: the acquisition process is simple, fast and easy to operate;

    Flexible: easily collected in the laboratory, clinic and even at home;

    Convenience: the collected saliva is stable, stored at room temperature and convenient for transportation;

    Extensive: especially suitable for children and patients who do not meet the conditions for blood sample collection;

    Safety: noninvasive sample collection to reduce the chance of infection;

    High efficiency:Convenient for automatic purification. We will collect more and better quality DNA