What is a Nasopharyngeal Swab?

Posted on 2021年9月23日2022年3月22日 by Dr. Lee

COVID-19 Nucleic acid swab test is a very effective and effective measure to prevent and control the new crown pneumonia epidemic.

The upper respiratory tract of human body includes nasal cavity, pharynx and throat.

That is to say, if people are infected with COVID-19, they can extract the nucleic acid of the virus in the nasal cavity, pharynx and larynx in 96 hours.

Therefore, we usually sample these locations in the rapid detection of new crown nucleic acids.

How to collect a nasopharyngeal swab?

Nasal swab sampling process

The first step is to collect secretions.

First, the medical staff will use a pharyngeal swab to wipe the subject’s nasal cavity or throat tonsils to collect salivary secretions.

nasal swab collection method

The second step is to reserve samples.

After collecting the sample, immerse the nasopharyngeal swab in the preservation solution and screw on the tube cover to retain the sample.

The third step is to send samples for inspection.

Then, put the samples into clean sealed bags and send them to relevant parts for testing.

The fourth step is nucleic acid extraction.

Relevant departments will send the samples to the designated laboratory for nucleic acid extraction experiment.

Step 5: fluorescent PCR nasopharyngeal swab.

After collecting nasal samples, the experimenter uses fluorescent PCR to detect whether there is amplification reaction.

That is, to judge whether it is negative or positive.

Step 6:Finally, Receive test report.

Finally, according to the fluorescent PCR reaction results of nucleic acid detection, the nucleic acid detection vehicle inspection report is obtained, and I can query the results.

Sampling swab material

The sampling swab used for nasopharynx collection consists of a narrow rod made of a short plastic rod, one end of which is covered with an absorbent material. Such as cotton, polyester or flocked nylon.

The swab used for virus sampling should directly contact the sampling part.

The material of the sampling head is closely related to subsequent detection.

And the material requirements of virus sampling swab will be very high.

The virus sampling swab head shall be made of polyester (PE) synthetic fiber or artificial fiber.

Forbidden to use calcium alginate sponge or wooden swab (including bamboo stick).

However, calcium alginate and wood components can also absorb proteins after immersion in the preservation solution, and even inhibit the subsequent PCR reaction.

What are the advantages of using flocked sampling Nasopharyngeal swabs?

1. Flocked swabs provide better sampling.

Flocking swabs are a good choice for rapid diagnostic tests because they can better collect cells or organisms at the collection site and ensure the rapid release of the whole cell.

2. Enhanced diagnostic sensitivity.

Professional flocked technology turns each fiber into a velvet like swab, thus creating hundreds of thousands of contact points to better collect and release the collected samples.

Compared with non aggregated swabs, the increase in the number of target cells helps to improve the sensitivity of rapid diagnostic tests.

3. Rapid elution.

Their stable and reliable performance and excellent absorption and elution ability.

4. Easy handling and transportation.

sterilized and packaged separately, and the sterile swabs.

Saliva Sample Collection Kit :Disposable saliva DNA RNA sampling tube

Posted on 2021年9月18日2022年3月22日 by Dr. Lee

What is saliva collection kit?

In recent years, with the development of medicine, the examination technology has become more and more high-end. And the examination items have become more and more accurate.

Saliva is a complex mixture, which not only contains various proteins. It also contains DNA, RNA, fatty acids and various microorganisms. Therefore, it is possible to diagnose diseases by detecting saliva. Gene detection and analysis.

Saliva collector plays an important role in the process of collecting, transporting and storing saliva samples.

It is noninvasive and easy to self-management, and reduces the risk of medical staff exposure.

Saliva Collection Kit suitable for SARS-CoV-2 sample collection.

Nucleic acid detection is the most widely used new crown detection method.

Including PCR nasopharynx swab, oropharyngeal swab and saliva sample test.

High precision. As long as the collection, breeding and separation of nucleic acids are successful, there is basically no false positive or false negative.

But the sampling staff are easy to be exposed and the risk is high; With one more step of extracting nucleic acid, unskilled technicians are likely to fail to extract, resulting in false negative, or pollute with other people's samples, resulting in false positive.

COVID-19 Testing:PCR swab test,antibody test, and antigen test difference

Posted on 2021年9月17日2022年3月22日 by Dr. Lee

COVID-19 Testing :PCR swab test,antibody test, and antigen test difference.

PCR swab test（Nucleic acid test） and antibody / antigen detection are important means to confirm COVID-19, and also one of the important processes for patients to diagnose. But do you know what their detection principle is? What are the sample types? What is the inspection process and what are the problems?

COVID-19 nucleic acid test

Nucleic acid detection has the characteristics of early diagnosis, high sensitivity and specificity. At present, real-time fluorescence quantitative RT-PCR is the most widely used technology. PCR swab testing is a very effective way to identify SARS-COV-2.

1. Principle of COVID-19 test kits?

The researchers used sampling swabs to collect samples of the virus, targeting the unique gene sequence of the virus.

By PCR amplification of the virus sample, the target DNA sequence we selected increased exponentially.

Researchers can combine the amplified DNA sequence with pre added fluorescent labeled probe fragments to generate fluorescent signals.

The more target genes amplified, the stronger the cumulative fluorescence signal.

In Virus-free samples, no target gene was amplified. Therefore, researchers can not detect enhanced fluorescence signals.

That is, we do not carry COVID-19.

Therefore, nucleic acid detection is actually to detect the accumulation of fluorescence signal to determine whether there is viral nucleic acid in the sample.

COVID-19 test kits_PCR swab test（Nucleic acid test）

2. What are the methods of covid-19 testing samples?

Generally, PCR nasal swab test, pharyngeal swab, nasopharyngeal swab, sputum, bronchial lavage fluid, alveolar lavage fluid, etc.

Sampling swab consists of a nylon flocked swab.what is a flocked swab?

3. Steps of PCR swab test for COVID-19.

At present, pharyngeal swabs are the main sampling method for the diagnosis of novel coronavirus infection. Correct sampling is the key to improve the accuracy of nucleic acid samples collected from pharyngeal swabs. What are the factors affecting the accuracy of nucleic acid samples collected from pharyngeal swabs? There are three main aspects:

Depth of throat swab collection?

How long is the throat swab in contact with the mucosa?

Pharyngeal swab sampling “dot to dot” or wipe?

(1) Oral and pharyngeal swab collection tips

1. Sampling position: sit or semi-sit in decuffine position, with head slightly raised and mouth open, and head resting on the wall or against the back of the chair.

2. Standard sampling operation: Sampling personnel press the tongue of the subject with tongue depressors.

Pharyngeal swabs should be performed on both sides of the pharyngeal tonsil with “Quick wipe up and down” and on the posterior pharyngeal wall with “Quick left and right wipe” . Repeat 3 to 4 times.

Avoid contact with tongue, teeth and gums.

If you feel uncomfortable, quit, take a break, and try again.

Oropharyngeal Sampling flocked swab diagram

3. Precautions: Drinking water before oral and pharyngeal swab test will dilute the virus attached to the pharynx, especially drinking hot water will inhibit the activity of the virus.

In order to improve the accuracy of pharyngeal swab nucleic acid detection, we recommend that subjects do not drink water 15-30 minutes before the detection, so as to ensure the high accuracy of pharyngeal swab nucleic acid test.

(2) Nasopharyngeal swab collection tips

1.The sampling personnel shall collect samples from the side of the subject, and the subject shall pull down the mask to expose only his nostrils. If there is sneezing reflex, the subject can cover it with elbow or paper towel.

nasopharyngeal sampling diagram

2. Standard sampling operation: “one insertion, two stops and three rotations” — measure the distance from the tip of the nose to the front of the ear, half of the insertion length, usually about 4 cm for adults; Stay for 15-30 seconds to absorb nasopharyngeal secretions (depending on tolerance, minimum stay for at least 3 seconds; Rotate the nasopharyngeal swab for 1 week and slowly remove it.

The above is for the reference of sampling medical staff and patients, let’s work together to defeat COVID-19!

(3)Store samples

Dip the flocked swab head into the cell preservation solution(Virus transport medium), and tighten the tube cap immediately after breaking the tail;

Storage, put the sample tube into a sealed bag for timely inspection, and the inspection process requires a strict transportation environment, 2-8 ℃ storage.

(4) PCR TEST

Researchers operate nucleic acid extraction, extract inactivated virus samples for subsequent nucleic acid detection, and can use automatic equipment such as nucleic acid extractor.

Fluorescence PCR nucleic acid detection, that is, on the machine detection, the extraction of fluorescence PCR amplification reaction, needs 70-80 minutes.

process pcr test

4. What are the problems in nucleic acid testing?

False negative, due to improper sampling, improper specimen preservation, the use of different types of specimens and the use of different manufacturers of reagents may lead to nucleic acid test results “false negative” and missed diagnosis;

■ High requirements for detection equipment or platform, high sensitivity RT-PCR instrument is expensive, laboratory cleanliness and operator requirements are also high;

■ Nucleic acid testing is time-consuming. It usually takes 4-6 hours to complete an RT-PCR test.

Therefore, when the nucleic acid test is negative.

Relevant personnel can increase the detection of IgM and IgG antibodies to make up for the shortcoming that nucleic acid detection is easy to cause missed diagnosis.

COVID-19 Testing serum antibody test

Seven days after novel coronavirus pneumonia, the serum specific antibodies gradually emerged. The first is immunoglobulin IgM antibody, and then IgG antibody.

Thus, an elevated IgM antibody indicates a recent acute infection, and an elevated IgG antibody indicates a previous infection.

The biggest advantage of serological testing lies in its convenience and short detection time, which can effectively break through the limitations of existing detection technology on personnel and sites and shorten the detection time.

Novel coronavirus infection can be diagnosed in suspected cases if the serum specific IgM and IgG antibodies are positive, the IgG antibody changes from negative to positive, or the recovery period increases by 4 times or more than the acute period.

antibody test sampling process_COVID-19 Testing

1. Principle of serum antibody test kit?

The sample is added to the sample adding hole, and the sample will move forward along the detection card under the action of chromatography.

If the sample contains new coronavirus IgM antibody, the antibody will combine with colloidal gold labeled virus antigen to form a sandwich complex, which will show a positive result.

If the sample contains New Coronavirus IgG antibody, the antibody will bind to colloidal gold labeled New Coronavirus antigen and form a sandwich complex, which will show positive results.

The detection card also contains a quality control line (line C) to judge whether the chromatography process is smooth.

2. What are the sample types for serum antibody detection?

Generally, it is blood, including serum, plasma and whole blood.

3.COVID-19 antibody test in three steps

1. Open the aluminum foil bag of the test card, take out the test card and place it horizontally on the table;

2. Use a pipette to absorb serum/plasma/whole blood sample and add it to the sampling site. Then use the same method to absorb buffer drops and add them to the sampling hole of the test card.

3. Wait 15 minutes and read the result.

4. What are the problems in serum antibody detection?

■ False positive, individual patients with rheumatoid factors in the blood, heterosinophilic antibodies, autoantibodies, drugs and tumor cells, etc., easy to cause cross-reaction test, so occasionally false positive results;

■ False negative, due to the existence of a certain window period of serum antibody detection methods and the sensitivity of the detection kit will appear false negative results.

Therefore, serum antibody test is only used as a supplementary test for suspected cases of novel Coronavirus nucleic acid negative test and cannot be used as a diagnostic indicator for screening alone.

The combined use of serum antibody detection and nucleic acid detection can help to improve the detection rate of the disease and find out the confirmed patients as much as possible, which is more conducive to the control of the epidemic.

Interpretation of results of nucleic acid combined with IgG and IgM antibody detection

COVID-19 Testing antigen detection

A novel coronavirus antigen test can novel coronavirus be detected directly in human body samples. The diagnosis is fast and accurate, with low requirements for equipment and personnel.

antigen test kit_SARS-CoV-2

1. Principle of antigen detection kit?

SARS-CoV-2 , N, E and S protein antigens stimulate virus cells to produce specific antibodies.

2. What are the types of antigen test samples?

Usually samples from infected sites, e.g. oropharyngeal swabs, nasopharyngeal swabs, sputum, serum, plasma, etc.

3.COVID-19 antigen test in four steps

1. Drop the sample treatment liquid into the sample treatment tube;

2. Stir the sample swab and squeeze the tube wall until the specimen is fully eluted into the treatment solution;

3. Take out the detection card and add sample to the round hole;

4. Wait 15 minutes and read the result.

4. What are the problems in antigen detection?

■ False negative, higher sensitivity of antigen detection is required. Because New Coronavirus mainly affects alveolar and other lower respiratory tract. Therefore, sampling from the upper respiratory tract of nasopharynx and oropharynx cannot determine whether the pathogen can be detected. Or the sample contains a small number of viruses. All these factors may cause missed detection.

■ The preparation process is tedious and time-consuming. The development of the antigen kit requires the preparation of the recombinant antigen first, and then the preparation of monoclonal antibody in mice, which takes about two or three months. If the prepared antibody performance is not good, it needs to be prepared again, which takes another two or three months.

The joint detection

The COVID-19 Testing nucleic acid / antibody / antigen has different focuses and can not be substituted for each other. Multiple detection methods are combined and complement each other. Combine molecular biology and immune level detection to give play to their respective advantages and improve sensitivity and specificity. It can effectively shorten the detection window period, improve the positive detection rate, and provide double guarantee for all possible risk groups.

reference：Zhengtu Li，Yongxiang Yi et al. Development and clinical application of a rapid IgM‐IgG combined antibody test for SARS‐CoV‐2 infection diagnosis. J Med Virol.2020；1-7

Mini flocked nose swab china factory and manufacturers

Posted on 2021年9月16日2021年12月21日 by Dr. Lee

Mini flocked nose swab china factory and manufacturersCell medical (Dongguan) Co., Ltd., flocked swab adopts vertical nylon fiber, which has stable and reliable performance and good adsorption and elution ability. Flocked swabs optimize sample collection and elution into the transmission medium. The swab also has a molded breakpoint that allows you to safely and easily disconnect the cotton swab. Our company provides multiple breakpoint options for different pipes.We can customize swab mold, swab packaging, sterile packaging and swab OEM services.

Mini flocked nose swab Specifications

Mini flocked nasal swab

Swab Length: 145mm±5mm
Flocked Tip Length: 8mm
Swab Tip Diameter: 8mm±0.2mm
Breakpoint: 80/90mm
Packing: Individual Sterile Package
Certificate: CE/FDA/ISO
OEM/ODM: Support
GMP Factory: YES
Supplying Ability: 500,0000pcs/ day

Application: Nasopharyngeal Sampling Swab

Mateiral: 100% Medical Grade Nylon

Sterilization: EO

Certificate: ISO13485, CE, FDA, SGS, MSDS, TDS.

What’s the use of a flocked nose swab？

Nasal swab detection is a kind of nucleic acid detection, which is used to detect the presence of viral nucleic acid in the body. So as to determine whether the human body infected with COVID-19.

Nasal swab detection requires collecting cells deep in the throat of the tested person and placing them in a Petri dish for observation. Because New Coronavirus is mainly transmitted through the respiratory tract,if the human body is infected with COVID-19, there will be a lot of viruses in the throat. Therefore, it is a more accurate way to detect the existence of COVID-19 by detecting the cells in the throat.

What is the method of mini nasal swab collection?

① Please keep the patient’s head still and remove the secretions on the surface of the anterior nostril;

② use a flocked swab or other tools to the distance from the nostril to the root of the ear and mark it with your fingers;

③ insert the swab gently and slowly through the nasal cavity;

④ swab until the finger mark or stay for several seconds after encountering resistance to absorb secretions; (generally stay for 15-30 seconds, and then rotate gently for 3 times);

⑤ gently rotate the swab and put it into the virus sampling tube (transport medium); Break the swab tail rod along the breaking point and place it completely in the tube. Tighten the pipe cover.

⑥ If you need to collect from two nostrils, you should use another swab.

⑦ Send to the inspection room for inspection as soon as possible.

⑧ Note the information of the collected person to avoid confusion.

Mini flocked nasal swab sampling display

China Sterile Sampling Swab factory and manufacturers

Posted on 2021年9月16日2022年3月22日 by Dr. Lee

China Disposable Sterile Sampling Swab factory and manufacturers

Guangdong Dongguan city Changan town comprehensive high-tech biotechnology company -Cell medical (Dongguan) Co., Ltd. , Nissan large amount of flocking sampling swab, Disposable Sterile Sampling Swab help COVID-19 prevention and control.

Our GMP factory _Cell medical (Dongguan) Co., Ltd.

Controlled environment Labor uniform strict control, Ensure DNA& RNA free product

Brief description

China Sterile Sampling Swab floced swab

Aseptic environment sampling swabs include aseptic environment sampling swabs, disposable sampling swabs, aseptic sampling swabs, disposable aseptic environment sampling swabs and disposable aseptic sampling swabs.

sterilization and packaging method

1. Sterilize the product with ethylene oxide

2. Adopted international paper plastic packaging convenient for sterilization.

3. Independent packaging, easy to use.

special storage conditions and methods

Stored in a cool, dry and clean environment with no corrosive gas at 2 ℃ ~ 30 ℃ and relative humidity of 40% ~ 90%.

Nasal swab: for medical institutions to collect virus and DNA samples infected in patients’ nasal cavity. Virus sampling swab: used for nose and throat sampling of respiratory and intestinal viruses such as influenza, swine influenza, avian influenza, hand, foot and mouth.

Product composition: ⑴ nylon flocked swab. That is, sterile sampling swab (for sampling, the release is up to more than 90%).

Product features:

① International paper plastic packaging convenient for sterilization.

② γ Ray sterilization to ensure sterility.

③ Each set of independent packing in the large packing box is convenient to use.

④Researchers can select different culture media (bacteria, viruses, mycoplasma, chlamydia) for different types of specimens.

China Sterile Sampling Swab (Cell medical ) advantages:

1. Collection swab features: the collection system adopts nylon flocked swab, which is non-toxic to microorganisms and can maximize the collection and release of samples.

2. A large number of clinical experiments show that compared with ordinary sterile cotton swabs, nylon flocked swabs have a better effect on the collection and transportation of clinical microbial samples.

Especially for those samples that cannot be submitted for examination in time and placed for too long.

Advantages of flocked swabs:

① The unique jet nylon fiber implantation technology increases the collection and release of specimens.

② The total length of the swab is 15cm, and the plastic rod has a unique breakable design.

③ Flocking swabs can collect more target analytes.

④ There is no residual specimen, so the specimen processing process can be accelerated.

⑤ Swabs are sterilized and packaged separately. Aseptic swabs packaged separately.

China universal viral transport medium factory manufacturers

Posted on 2021年9月16日2022年3月30日 by Dr. Lee

China universal viral transport medium factory and manufacturers

Product specification

VTM KIT_Virus sampling tube

[common name]: disposable virus sampling tube, viral transport medium kits,universal viral transport media.

[specification and model]: 3ml / piece, 100 pieces / bag

[main structure and performance]

It usually consists of swabs and/or preservation tubes containing viral transport medium.

[scope of application]

For sample collection, transportation and storage.

[action principle]

After sterilizing the sampling tube, add virus stable components such as Hank’s base solution, which can maintain the activity of virus in a wide temperature range, reduce the decomposition speed of virus and improve the positive rate of virus isolation.

Description

Viral Transport Medium (VTM) allows the safe transfer of viruses, chlamydia and mycoplasma for further research, including conventional cell culture methods, diagnostic tests, and molecular biology techniques.

Commercially prepared viral transport media are available in a screw cap plastic tube containing buffered proteins (serum, albumin or gelatin) and antibiotics.

Viral transport medium composition

Our viral transport media contains Hanks Balanced Salt Solution (HBSS) with Calcium and Magnesium and contains heat-inactivated Fetal Bovine Serum, Gentamycin and Amphotericin B.

The composition and the manufacturing of the Viral Transport Medium follow the WHO and the CDC recommendations.

[contraindications, precautions, warnings and suggestive instructions]

1. After sampling with a virus sampling tube, the sampler shall submit it for inspection as soon as possible.

At the same time, the transportation personnel shall immediately transport the virus samples at a low temperature of 2-8 ℃.

The results showed that the virus samples could be stored at 2-8 ℃ for 48 hours; But for long-term storage, below – 20 ℃ and – 70 ℃.

If the sampler does not immediately transport at low temperature or store at the specified temperature after sampling. It will affect the positive rate of virus sample separation in the later stage.

2. prohibited to use it for the sampling of bacterial samples. The antibiotics contained in the sampling solution can inhibit bacteria.

3. forbidden to directly contact the collected patient, and forbidden to sample the patient after wetting the swab.

4. The sampling personnel shall collect virus samples in strict accordance with the sampling procedures to ensure the accuracy of sampling location. At the same time, the sampling intensity is uniform and appropriate, otherwise, it will affect the positive rate of virus isolation.

5. It is forbidden to use the expired or damaged product package.

6. The experimenter shall test the samples in a laboratory meeting the safety level.

Factory profile

Virus transport medium factories, Cell medical is a professional manufacturer of sampling swabs and disposable virus sampling tubes (viral transport medium).

The factory is located in Dongguan, China, with a daily production capacity of more than 1 million sets, aseptic filling technology and aseptic swab production technology.

China universal viral transport medium factory and manufacturers_Cell medical (Dongguan) Co., Ltd.

China VTM tube factory GMP factory_Cell medical (Dongguan) Co., Ltd.

Cell medical (Dongguan) Co., Ltd., vtm kit factories, vtm swab factory, produce flocked swabs, VTM kits, virus transport medium, universal viral transport media, with multiple automatic production lines.

We support custom production, OEM and so on, and develop products with customers.

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Email:flockedswabs@gmail.com

What is genetic testing?

Posted on 2021年9月9日2021年9月9日 by Dr. Lee

What is genetic testing?

Definition of gene

What we usually call genes is a collection of genes in human cells, called the genome.

Every cell in our body has a complete genome. Inherited from parents and passed on to children.

Genes make people different from animals, make families different from families, and brothers and sisters are similar but different.

These subtle differences often do not exceed 0.5% of genes.

But determine different appearance, personality, genetic characteristics, and our adaptation to the external environment.

Why genetic testing?

It must be noted that various human traits and diseases are the result of the joint action of genes and environment .

Genes only play a partial role in traits, and whether gene expression is also greatly affected by the internal and external environment.

Therefore, gene test reports can not be directly used as the basis for clinical treatment, and it is not advisable to judge the role of genes without individual environment.

On this premise, the gene test report still has a certain reference significance for understanding various characteristics of the human body.

Understand that they carry hereditary pathogenic genes

We can know whether we carry pathogenic genes through genetic testing. For example, people with a family history of genetic diseases, cancer and hypertension. According to the test results, adjust dietary health care and living habits to avoid the occurrence of diseases. If both pregnant couples carry the pathogenic gene of recessive disease at the same time. They can pay attention and conduct further investigation when preparing for delivery.

Select drugs correctly to avoid drug waste and adverse drug reactions

Due to genetic differences, individuals will respond differently to drugs. When taking the same kind of drugs, some people will feel very effective, while others will not only be ineffective, but also have toxic and side effects. When some people use certain drugs, they may also have drug allergy. Through gene testing, we can know everyone’s gene situation, which may play a guiding role in drug use to a certain extent. Again, the drug test report can not be directly used in clinic. The drug selection and use in the treatment of diseases need to follow the doctor’s advice.

Provide scientific health management

Many adverse environmental factors and bad living habits will make the human body more prone to diseases. People can understand the occurrence tendency of individuals in different diseases through genetic testing, and make corresponding life adjustment or intervention in order to reduce the risk and delay the occurrence of diseases.

What is the principle of gene testing?

To understand the principle of gene detection, we need to understand the following questions:

Reasons for gene testing

Each person’s human nucleus has 23 pairs of chromosomes, which are composed of DNA and ribosomal proteins. All chromosomes have 3.16 billion base pairs.

When the same base pair position changes (this situation is generally called single nucleotide polymorphism, or SNP), which has a certain impact on human genetics and health, this SNP is the locus in the gene report. At present, there are nearly more than 100 million known or named SNPs. Most of the differences between human DNA are SNP differences, which is about 0.5% of the total base pairs of human DNA. A large part of gene detection is based on SNP.

Samples for genetic testing

Normal human samples required for gene testing include saliva sampling and blood sampling. For different examination items, excreta sampling and cell slice sampling are also included.

Methods of gene testing

There are several ways to measure related base pair differences (SNPs): PCR, chip, exon, and genome-wide. The number of SNPs detected is dozens, hundreds of thousands, tens of thousands, and all SNPs.

Disposable DNA sampling flocked swab

Oral DNA sampling swab_flocked swab cm-98000

1.Specially designed for micro DNA samples at the crime scene, especially for micro samples such as sweat, semen, blood, dandruff, oral cells, etc.

2. It can quickly absorb a small amount of samples and has high release aging rate.

3. The front end is sharp enough to extract cells from the victim or suspect’s fingernails.

4. The swab has a breakpoint, which is convenient for the automatic extraction workstation to extract samples.

5. The whole swab is packed in a transparent plastic tube to avoid biological pollution.

Who can do genetic testing?

① People with family disease history;

② People with bad living habits;

③ People exposed to chemical pollution, heavy metal pollution, nuclear pollution and toxic and harmful substances for a long time;

④ Friends who pay attention to health;

Hello!

When many diseases are diagnosed, it’s too late. Do a good job in disease prevention to make us healthy for a longer time!

COVID-19 Testing: Method of collecting virus swab samples

Posted on 2021年9月9日2021年9月17日 by Dr. Lee

COVID-19 Testing: virus swab

According to New Coronavirus’s pneumonia diagnosis and treatment plan (trial version fourth), to diagnose suspected cases of New Coronavirus pneumonia, we need one of the following etiological evidence:

1. Respiratory tract specimens or blood samples were detected by real-time fluorescence RT-PCR for New Coronavirus nucleic acid positive.

2. Novel coronavirus is highly homologous to respiratory tract specimens or blood samples.

Therefore, novel coronavirus pneumonia is an indispensable part of the specimen collection. So, where to collect specimens? How to collect specimens? How can medical staff protect themselves during collection? What should I do after collection?

How to obtain a virus swab specimen?

We all know that the collection method of virus swab specimen is basically to use the swab to sample the infected place or part of the virus, and then put it into the virus sampling tube containing preservation solution without touching other places, so as to conduct virus detection later.

Specimen type:

1. Upper respiratory tract specimens: including pharyngeal swab, nasal swab, nasopharyngeal extract, etc.

2. Lower respiratory tract specimens: including deep expectoration fluid, respiratory tract extract, bronchial lavage fluid, alveolar lavage fluid and lung tissue biopsy specimens.

3. Blood samples: try to collect anticoagulants in the acute stage within 7 days after onset. The collection volume is 5 ml, preferably fasting blood.

4. Serum samples: try to collect double serum in acute stage and recovery stage.

5. Conjunctival specimens: conjunctival swab specimens shall be collected for cases with ocular infection symptoms.

6. Stool samples: Patients with diarrhea symptoms need to collect stool samples.

COVID-19 Testing: Common virus swab sampling methods

Methods of throat swab collection

Inform the patient of the purpose of examination, collection method and collection time. It is best to collect specimens before using antibiotics.

Objective: To study the clinical effect of the method

Secretions were collected from pharynx or tonsil for bacterial culture or virus isolation to assist in clinical diagnosis and treatment.

Prepare sampling supplies:

Disposable sampling swab, virus sampling tube, tongue depressor and biological bag

Operation method:

(1) Ask the patient to sit down, tilt his head back, open his mouth and make a “ah ~” sound

(2) The sampling personnel shall fix the patient’s tongue with a tongue depressor, and use a disposable pharyngeal swab to pass through the root of the tongue to the posterior pharyngeal wall, tonsil recess and lateral wall;

(3) Repeatedly wipe 3-5 times to collect mucous membrane cells or sputum

(4) . gently remove the swab to avoid touching the tongue, the suspension, the oral mucosa and saliva

(5) Put the swab into the virus sampling tube gently and vertically. The sampling personnel shall disconnect the tail of the flocking swab and leave the tail of the swab completely in the tube.Be careful that the swab does not touch the wall of the tube.

(6) . tighten the cap, mark it, and seal it in the biosafety bag.

(7) The sampling personnel shall send the samples to the laboratory for testing in time. If the sampling personnel cannot send the samples for inspection in time, they shall store them at low temperature

Methods: nasal swabs were collected

Nasopharyngeal swab_COVID-19 Testing: Method of collecting virus swab samples

(1) Ask the patient to sit down with his head slightly back and remove the secretion from the front nostril first;

(2)measure the distance from nostril to ear base with swab and mark with finger

(3)gently insert the swab into the nostril until it is marked or stopped when there is obstruction. Let the swab stay in the nose for 15-30 seconds, and then rotate it gently three times.

(4) slowly pull out the nasal swab and quickly put it into the virus sampling tube. Then break the swab, leave its tail and place it completely in the tube.

(5) tighten the cap, mark it, and seal it in the biosafety bag.

(6) If we need to collect from both nostrils, we should use a swab.

(7) It should be sent to the laboratory for testing in time. If it can not be sent for inspection in time, it should be stored at low temperature.

Generally speaking, COVID-19 testing: the main methods of collecting virus swabs are nasopharyngeal sampling and oropharyngeal sampling.

Is COVID-19 pneumonia more infectious than SARS?

The effectiveness of infectious disease transmission depends on its basic regeneration index. Novel coronavirus pneumonia is more infectious than SARS at present, with a basic regeneration index of SARS higher than that of SARS.

The novel coronavirus pneumonia is a new disease, and the population is generally immune. Everyone is susceptible and susceptible to infection. The incubation period of COVID-19 also has the ability to transmit, and it has longer infection time. The novel coronavirus pneumonia novel coronavirus pneumonia is also typical of the clinical symptoms, and asymptomatic infections are also contagious, so the infection of new crown pneumonia is higher than SARS.

About us

Cell medical (Dongguan) Co., Ltd. is a swabs manufacturer. We produce and sell virus sampling related products. Such as virus sampling flocked swabs, VTM kits and virus transport media, and have multiple automatic production lines. We support customized production, OEM, etc., and develop products with customers.

Disposable virus sampling kits

Posted on 2021年9月8日2022年3月22日 by Dr. Lee

Disposable virus sampling kits

Cell medical (Dongguan) Co., Ltd. _China swab manufacturer.Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.

We produce flocked swabs, VTM kits, virus transport medium, Disposable virus sampling kits,with multiple automatic production lines.We support custom production, OEM and so on, and develop products with customers.

Email:flockedswabs@gmail.com

Nasopharyngeal Swab & Oropharyngeal Swab

Item No.#	Description	Sterilization
CM-98000	Oropharyngeal Specimen Collection flocked Swab, Single swab packaging, Sterile,Breakpoint 30mm	EO
CM-96000	Nasopharyngeal Specimen Collection flocked Swab, Single swab packaging, Sterile,Breakpoint 80mm	EO
CM-93050	Throat Specimen Collection flocked Swab, Single swab packaging, Sterile,Breakpoint 80mm	EO
CM-96000N	Nasopharyngeal Specimen Collection flocked Swab, Single swab packaging, Sterile,Breakpoint 90mm	EO
CM-98000T	Medical Flocked Swab With Test Tube, with Individually Wrapped and Sterile Buccal（oral) Swabs	EO
CM-93050T	Specimen Collection Swab tube, Individual Sterile Package Oropharyngeal Swabs	EO
CM-96000T	Nasal Sample Collection Kits,flocked Swab With Test Tube,ndividually Wrapped and Sterile	EO

Ethylene oxide sterilization, irradiation sterilization and independent packaging.

Supply Ability:500,000-1,000,000 Pieces per Month

Disposable sampling swab application

The top of the flocked swab is made of nylon fiber. The nylon fiber is successively attached to the surface of the medical ABS rod through the electrostatic field. The layer is completely parallel and perpendicular to the surface of the internal rod for DNA collection.

The collection system adopts nylon flocked swab, which is non-toxic to microorganisms and can maximize the collection and release of samples.

A large number of clinical experiments show that compared with ordinary sterile cotton swab, nylon flocking swab has better effect on the collection and transportation of clinical microbial samples

This is especially true for those specimens that cannot be submitted for examination in time and placed for too long.

Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.

Product features

1.Non invasive,Non-irritating；

2.Outstanding sample collection and release ability;

3.Rapid Absorption;

4. Increased Assay Sensitivity;

5.Perpendicular Nylon Fibers;

6.Certified Free Of Inhibitors And Interference.

Our FDA & CE certificate can ensure effective and legal customs clearance in your country.

VTM (Viral Transport Medium):Including inactivated and non inactivated.

Quality Certification:MSDS,CE,ISO

Our virus transport medium is based on Hanks equilibrium salt solution (HbSS).

It contains calcium and magnesium, heat inactivated fetal bovine serum, gentamicin and amphotericin B.

The composition and preparation of virus vectors follow the recommendations of who and CDC.

The product is provided in a sterile 13 ml flat bottom tube with or without swabs, providing the greatest range of possibilities for collecting samples.

Inactivated Viral Transport Medium(ITM).

This product is designed for the inactivation of a variety of viruses, such as clinical 2019-ncov, influenza, avian influenza, hand-foot-mouth, measles virus, norovirus, rotavirus and other virus specimens, and for the transportation of the virus nucleic acids. It is compatible with various viral nucleic acid extraction kits available on the market and can achieve seamless connection with downstream nucleic acid extraction.

1. It can not only inactivate the virus, but also prevent nucleic acid degradation.

2. Room temperature transmission

3. The samples can be stored at room temperature for 20 days.

4. It is used to collect, store and transport viruses, chlamydia, mycoplasma and Ureaplasma.

Model	Specification
VTM-B-CM010120S	2With Nasal flocked Swab& Throat Swab ; 1ml inactivated medium ;2ml tube
VTM-B-CM010111S	With Nasal Swab ; 1ml inactivated medium ;2ml tube
VTM-B-CM010112S	With Throat Swab ; 1ml inactivated medium ;2ml tube
VTM-B-CM020220S	2With Nasal Swab& Throat Swab ; 2ml inactivated medium ;5ml tube
VTM-B-CM020211S	With Nasal Swab ; 2ml inactivated medium ;5ml tube
VTM-B-CM020212S	With Throat Swab ; 2ml inactivated medium ;5ml tube
VTM-B-CM020320S	2With Nasal Swab& Throat Swab ; 3ml inactivated medium ;5ml tube
VTM-B-CM020311S	With Nasal Swab ; 3ml inactivated medium ;5ml tube
VTM-B-CM020312S	With Throat Swab ; 3ml inactivated medium ;5ml tube
VTM-B-CM040320S	2With Nasal Swab& Throat Swab ; 3ml inactivated medium ;10ml tube
VTM-B-CM040311S	With Nasal Swab ; 3ml inactivated medium ;10ml tube
VTM-B-CM040312S	With Throat Swab ; 3ml inactivated medium ;10ml tube
VTM-B-CM040520S	2With Nasal Swab& Throat Swab ; 5ml inactivated medium ;10ml tube
VTM-B-CM040511S	With Nasal Swab ; 5ml inactivated medium ;10ml tube
VTM-B-CM040512S	With Throat Swab ; 5ml inactivated medium ;10ml tube

Non inactivated Viral Transport Medium(ITM).

Application:Nucleic acid detection and virus isolation.

1. Low temperature non freezing storage, does not damage the shell of the virus, and is convenient for long-distance transportation.

2. Applicable to all kinds of swab samples, including oral swab, nasal swab, throat swab, etc

3. It can be used for H1N1 influenza virus and any other virus that can be sampled with a swab.

4. The viral nucleic acid can be extracted with the lentivirus DNAOUT or the lentivirus RNAOUT.

5. Antibiotics in the sampling solution can effectively prevent bacterial and fungal contamination.

6. Bovine serum albumin is added to the sampling solution, which can protect the virus sample and improve the separation rate.

Model	Specification
VTM-A-CM010120S	2With Nasal flocked Swab& Throat Swab ; 1ml Non inactivated medium ;2ml tube
VTM-A-CM010111S	With Nasal Swab ; 1ml Non inactivated medium ;2ml tube
VTM-A-CM010112S	With Throat Swab ; 1ml Non inactivated medium ;2ml tube
VTM-A-CM020220S	2With Nasal Swab& Throat Swab ; 2ml Non inactivated medium ;5ml tube
VTM-A-CM020211S	With Nasal Swab ; 2ml Non inactivated medium ;5ml tube
VTM-A-CM020212S	With Throat Swab ; 2ml Non inactivated medium ;5ml tube
VTM-A-CM020320S	2With Nasal Swab& Throat Swab ; 3ml Non inactivated medium ;5ml tube
VTM-A-CM020311S	With Nasal Swab ; 3ml Non inactivated medium ;5ml tube
VTM-A-CM020312S	With Throat Swab ; 3ml Non inactivated medium ;5ml tube
VTM-A-CM040320S	2With Nasal Swab& Throat Swab ; 3ml Non inactivated medium ;10ml tube
VTM-A-CM040311S	With Nasal Swab ; 3ml Non inactivated medium ;10ml tube
VTM-A-CM040312S	With Throat Swab ; 3ml Non inactivated medium ;10ml tube
VTM-A-CM040520S	2With Nasal Swab& Throat Swab ; 5ml Non inactivated medium ;10ml tube
VTM-A-CM040511S	With Nasal Swab ; 5ml Non inactivated medium ;10ml tube
VTM-A-CM040512S	With Throat flocked Swab ; 5ml Non inactivated medium ;10ml tube

Disposable virus sampling kits sampling process

Nasopharyngeal swab sampling_flocked swabs

Oropharyngeal swab sampling

Nasal swab for flu,Rapid antigen nasal or throat swab

Posted on 2021年9月8日2022年3月22日 by Dr. Lee

Nasal swab for flu_Rapid antigen (nasal or throat swab) test

Influenza is common in winter and spring. The clinical manifestations are mainly systemic poisoning symptoms such as high fever, fatigue, headache, cough and systemic muscle pain, while the respiratory symptoms are mild.

Influenza viruses are prone to mutation, infectious, susceptible to population and high incidence rate. They have caused many outbreaks in the world, and are important public health issues worldwide.

Why should we detect and prevent influenza?

According to the World Health Organization (WHO), seasonal influenza can cause 3-5 million severe cases and 290000-650000 deaths worldwide every year.

The researchers believe that children under the age of 5 are at high risk of severe influenza, but children under the age of 2 are at the highest risk, and infants under 6 months have the highest hospitalization rate and mortality.

Pregnant women, infants, the elderly, patients with chronic basic diseases and other high-risk groups have a higher risk of serious diseases and death after suffering from influenza.

Nasal swab for flu : common influenza test method

Nasopharyngeal sample collection

Influenza is an acute inflammation of the respiratory tract caused by the patient’s infection with influenza virus. The patient can have a series of symptoms such as cough, expectoration, fever, sore throat, stuffy nose, runny nose and general fatigue. In case of influenza, we should conduct relevant examinations in time to further clarify the diagnosis, including the following tests:

First, blood routine test, patients with influenza can promote the increase of lymphocytes. If combined with bacterial infection, there can be the increase of leukocytes and so on.

Second, scientists can culture throat swabs of influenza viruses. In throat swab culture, researchers can detect the related virus.

Third, influenza patients can do second-generation sequencing, including sputum second-generation sequencing, which can determine the type of virus infected.

Fourth, influenza patients can do the detection of respiratory pathogens to further clarify the pathogens of infection.

At present, the two most common influenza tests are rapid antigen diagnostic test and influenza virus cell culture test. Both tests rely on Nasopharyngeal swabs, pharyngeal swabs, etc. for virus sampling. The most commonly used sampling swab is nylon flocking swab.

Nasal swab for flu:Application

It is suitable for the detection and sampling of COVID-19, influenza virus , hand foot mouth disease virus, COVID-19, measles rubella and other types of viruses.

About nasal swab sampling

Why use flocked nylon swabs?

flocked swabs_Cell medical (Dongguan) Co., Ltd.

Flocked nylon swab: excellent sample collection / release ability, release rate > 90%.

Conventional fiber swab: the sample is intercepted by the gap in the fiber mass. That is, the sample is penetrated, diluted and intercepted in the fiber. Acquisition is slow and release is slower.

The sampling time of flocked swab is 3-5 seconds, while that of traditional swab is 15-120 seconds or even longer.

This caused pressure on the staff during the diagnosis process. Only by extracting samples faster can we complete the collection and analysis more efficiently.

Precautions for patients with nasopharyngeal swab:

① Please do not take antibiotics a few days before making nasal swabs;

② Do not use disinfectant mouthwash or smear the focus a few hours before nasal swab.

③ Do not eat, smoke or drink a few hours before doing nose swab.

Precautions for Sampler:

① . the sampler shall stand on the side of the patient for sampling.

② The patient is required to pull down the mask to expose only his nostrils. In case of sneezing reflex, the patient can cover it with elbows or paper towels.

If the sampler is not directly in front of the patient, the exposure risk will be lower.

③ If the patient’s turbinate is hypertrophic, the nasal tract is narrow, and the insertion of the nasal swab feels resistance. Try to change one side of the nasal cavity or directly change to oropharyngeal swab collection.

④ Those with nasal allergy symptoms are easy to induce sneezing. It is recommended to take oropharyngeal swab samples.

⑤ Remember to wear a protective mask / screen. For individuals who are highly suspected or basically diagnosed, or who have severe cough and hiccup, they should put on protective clothing.

⑥ After sampling, replace gloves, disinfect hands, and spray disinfectant where patients have touched.

Finally, we should pay attention to nasal swab for flu in special times. Remember to wear masks and personal hygiene when going out. Once you find the virus, please isolate it to avoid re transmission.