Respiratory Specimen Collections

Respiratory Specimen Collections

Respiratory Specimen Collections

Today, with the increasingly severe international COVID-19 epidemic, the correct collection of respiratory samples has become an important prerequisite for ensuring the accuracy of nucleic acid detection results.

However, how to correctly collect respiratory samples? Cell medical (Dongguan) Co., Ltd. summarizes as follows:

Why should respiratory tract samples be collected standardized and correctly?

1. Clear diagnosis;

2. Observe the condition and treatment effect;

3. Guide the formulation of treatment measures;

4. Sample quality directly determines the reliability of test results.

 What are the types of respiratory samples?

1. Upper respiratory tract samples (including swabs, nasal specimens, nasopharynx extracts, pharyngeal gargle and nasal lotion, etc.);

2. Lower respiratory tract samples (such as sputum, tracheal aspirate, lung lotion, etc.);

When is the best time to collect respiratory samples?

Respiratory tract samples were collected as soon as possible after symptoms appeared;

Collect as much as possible within 72 hours of the patient’s fever;

Try to collect before the use of antiviral drugs;

If possible, collect multiple times in multiple days;

What sampling tools are needed to collect respiratory tract samples?

1. Virus sampling kits, including flocked swab, virus sampling tube, virus transport medium, etc;

2. Refrigerated transport box;

3. Personal protective equipment: protective mask (N95), protective glasses.

 How to collect respiratory tract samples?

Oropharyngeal swab sampling process:

Oropharyngeal swab sampling pictures

Oropharyngeal swab sampling pictures

1. The sampler first let the patient sit down, then let the patient tilt his head back and open his mouth.

2. Press the front 2 / 3 of the patient’s tongue with a tongue depressor, and wipe the posterior pharyngeal wall and bilateral tonsils with a nylon flocked swab for 3-5 times to avoid touching the tongue.

3. Take out the rear sampling tube, break the plastic handle at the contact part of the hand, soak the swab into the sampling solution, and tighten the tube cover.

Nasopharyngeal swab:

"nasal

Note : do not use too much force, but try to be as deep as possible, usually more than 5cm, stay for several seconds to absorb secretions, gently rotate and take out the swab, place the sampling tube, break the plastic ellipse at the contact part of the hand, and soak the test piece into the sampling solution, Tighten the pipe cover.

Respiratory Specimen Collections preservation requirements

Those that can be detected within 24 hours can be stored at 4 ℃ and frozen at – 70 ℃ for more than 24 hours.

 

Swab test

Swab test | Disposable virus sampling swab

Swab test | Disposable virus sampling swab

Disposable virus sampling swab, I believe you are no stranger in the past two years. Because most of the detection tools used for nucleic acid test are disposable sampling swabs. Usually, we see most nasal swabs and pharyngeal swabs. But disposable virus swabs are not only useful for nucleic acid testing. What else can a disposable sampling swab do? Let’s get to know.

What can a disposable virus swab be used for?

In addition to nucleic acid detection, scientists can also use disposable sampling swabs for microbial collection. They are usually used for virus detection and research in hospitals or laboratories. Researchers use disposable sampling swabs to detect biological samples in natural cavities of human or animals. Such as throat, nose, mouth, vagina and so on. It is necessary to collect oral epidermal cells and nasal virus samples with a sampling swab, store the cells and samples in the sampling tube and transfer them to the laboratory for examination.

Oropharyngeal swab sampling

Oropharyngeal swab sampling

Operation (use) method of disposable sampling swab:

Take pharyngeal swab as an example:

① Tear open the packaging bag of the disposable sampling swab and take out the disposable sampling swab from the bag;

Note: please do not let the disposable sampling swab touch the place outside the throat to avoid pollution.

② Put a disposable sampling swab into the mouth and quickly wipe the palatal arch and throat (or lesion) on both sides

③ Take out the swab, put it into the disposable virus sampling tube, break the disposable sampling swab at the broken place and discard the tail;

④ Tighten the sampling tube, put the virus sampling tube into the biosafety bag and submit it for inspection in time.

Note: please do not shake the virus sampling tube excessively to avoid dilution and loss of samples. After sampling, do not touch other objects with a disposable sampling swab.

How to carry out COVID-19  nasal swab test?

The doctor shall wear protective clothing, mask, gloves and mask to let the patient take a proper position.

Gently insert the flocked sampling swab into the nose, rotate it for three times, take it out and put it into the sample tube with protective solution, and screw the cover. Then label it, write the date, and send it to the laboratory for nucleic acid test of nasopharyngeal swab.

 

What is a COVID-19 swab test?

Swab test, namely nucleic acid test. The substance test by nucleic acid is the nucleic acid of virus. Nucleic acid detection is to collect the presence of viruses in the respiratory tract, blood or feces of patients, and determine whether they are infected by COVID-19. Therefore, once detected as nucleic acid “positive”, it can prove that there is a virus in the patient’s body.

SARS-CoV-2 nucleic acid detection process.

The usual sample types include throat swabs, nasal swabs, sputum, bronchoalveolar lavage fluid, alveolar lavage fluid and so on.

The sampling personnel shall conduct PCR detection as soon as possible after obtaining patient samples.

After the testing institution receives the sample, the laboratory personnel shall extract nucleic acid from the sample. And the nucleic acid extraction reagent shall use the nucleic acid extraction kit specified in the approved product manual.

Disposable virus sampling swab material.

At present, because of its stable and reliable performance and excellent absorption and elution capacity, nylon flocked swabs are widely used in COVID-19 sampling.

Swab test result

Swab testing for novel coronavirus pneumonia usually takes 4-6 hours to produce results, while rapid examination takes 2-3 hours. However, the reagents used in each hospital are different, so the time of results is also different.

COVID-19 positive results:Maybe infected.After 24 hours of self isolation, the positive patients need to conduct swab test again.

If the two results are positive, the next step is to improve CRP, blood routine, chest CT and other tests to determine whether COVID-19.

When COVID-19 nucleic acid test is negative once, it should be combined with the clinical symptoms and epidemiological contact history of patients. Because a COVID-19 nucleic acid swab test is negative, it can not completely exclude the infection of COVID-19.

 

Comparison of advantages of various testing methods of COVID-19

Comparison of advantages of various testing methods of COVID-19

advantages of various testing methods

The detection methods of COVID-19 mainly include nucleic acid detection, antibody detection and antigen detection. Due to the low detection rate of antigen, the SARS-CoV-2 detection mainly focuses on antibody and nucleic acid detection. Nucleic acid detection has the characteristics of early diagnosis, high sensitivity and specificity; However, antibody detection is convenient and rapid, which can be used as a supplementary means of nucleic acid diagnosis. Due to the limitations of “false positive” and “false negative” antibody detection, it is not suitable for general population screening such as returning to work,  and school, nor for epidemiological investigation in low epidemic areas.

NAT Detection process

The detection procedure needs to go through five steps: sampling, sample retention, preservation, nucleic acid extraction and computer detection.

First, collect samples according to the instructions of the kit. The sample types include pharyngeal swab, nasal swab, sputum, bronchial lavage fluid, alveolar lavage fluid, etc.

Because RNA is easy to degrade, flocked swabs without RNase and storage tubes without RNase are used when collecting samples.

After obtaining the patient’s sample, it needs to be tested as soon as possible. If it cannot be tested immediately, it needs to be packaged at low temperature and sent to a special testing institution for testing.

After receiving the sample, the testing institution shall extract the nucleic acid from the sample. The nucleic acid extraction reagent shall use the nucleic acid extraction kit specified in the approved product manual.

Finally, fluorescence PCR nucleic acid detection, that is, machine detection. It takes 70-80 minutes to amplify the extract by fluorescence PCR.

Sample collection, oropharyngeal swab or nasopharyngeal swab

Sample collection, oropharyngeal swab or nasopharyngeal swab

NAT Detection process Nucleic Acid Testing

NAT Detection process Nucleic Acid Testing

Antibody detection

After the virus invades the human body, the human body will produce corresponding specific antibodies for defense.

According to different molecular structures and antigen specificity, scientists divide immunoglobulins into five categories: IgG, IgA, IgM, IgD and IgE.

After the antigen enters the human body for the first time, plasma cells secrete antibodies after a certain incubation period. IgM first appeared, but the antibody had short maintenance time, low concentration and low affinity. It lasted for several days to several weeks in the blood. It was a diagnostic index of acute infection; When IgM is about to disappear, the content of IgG reaches the peak. IgG appeared late, but its concentration was high, its maintenance time was long and its affinity was high. The positive serum IgG indicates the middle and late stage of infection or previous infection.  But the antibody content increases rapidly in the short term, which may be several times to dozens of times higher than the original antibody content. It is mainly IgG, which lasts a long time in the body, and IgM rarely increases.

In this epidemic, the researchers studied patients with covid-19 and found that after the virus invades the human body, IgM antibody takes about 5-7 days to produce and IgG antibody takes 10-15 days to produce.

Antibody detection kits-advantages of various testing methods

Antibody detection kits-advantages of various testing methods

Comparison of advantages and disadvantages of two different detection methods

Compared with the serological detection of antibodies, Nucleic Acid Testing can detect patients in the window stage.

Early detection of infected persons is the “gold standard” for COVID-19 detection, but it requires higher detection equipment or platform.

The high sensitivity RT-PCR instrument is expensive, and the laboratory cleanliness and operator requirements are higher. In addition, nucleic acid detection takes a long time. Considering the sample transportation and sample backlog, the results can be reported as soon as 24 hours.

Compared with nucleic acid detection, blood samples detected by antibody serology are easier to obtain. If the RNA of the virus detected by nucleic acid is the direct evidence of the existence of the virus, the antibody detection is the antibody stimulated in the patient’s blood, which is indirect evidence and has a suggestive effect on the clinic.

When nucleic acid detection is negative, adding IgM and IgG antibody detection can make up for the shortcoming that nucleic acid detection is easy to cause missed diagnosis.

About us

Cell medical (Dongguan) Co., Ltd., a professional VTM kits and swabs manufacturer, has many years of experience in medical application molding and flocked treatment heads and swabs.

Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.Certificate:FDA,CE,EU,Free Sale,Export license based on (GMP).

We produce flocked swabs, VTM kits, Virus transport medium, with multiple automatic production lines.We support custom production, OEM and so on, and develop products with customers.

  • E-mail:flockedswabs@gmail.com

     

    Is anal swab nucleic acid test more accurate than nasopharyngeal swab?

    Is anal swab nucleic acid test more accurate than nasopharyngeal swab?

    Is anal swab nucleic acid test more accurate than nasopharyngeal swab?

    Nucleic acid detection:generally carried out by sampling pharyngeal swabs in the nasal cavity and oral cavity.

    Now began to increase the anal swab test, we are also curious about this test method. So, is anal swab nucleic acid detection more accurate than nasopharyngeal swab? How to sample for anal swab test?

    Is the nucleic acid detection of anal swab more accurate than that of nasopharyngeal swab?

    In the screening of New Coronavirus pneumonia, we mainly used the oropharyngeal swab nucleic acid test. Because the reason is convenient and quick, suitable for large-scale screening. The accuracy of nasopharynx swab for new crown nucleic acid detection is higher than that of oropharyngeal swab, but the human body feels uncomfortable.

    In last year’s test, researchers found that many infected people had negative pharyngeal nucleic acid.But their anal swabs were still positive for longer.

    The combination of the two in nucleic acid detection can improve the detection rate and reduce the proportion of missed diagnosis.

    However, anal swab sampling is inconvenient and not suitable for large-scale screening and testing in the community.

    For some asymptomatic or mild infections, the condition recovers quickly after infection. And the pharyngeal nucleic acid may not be detected in 3 to 5 days.

    The researchers found that the duration of nucleic acid positive in feces or anal swabs of some infected people was longer than that in the upper respiratory tract.

    Therefore, increasing anal swab nucleic acid detection can improve the detection rate of infected patients and reduce missed diagnosis.

    Although nucleic acid detection has the advantages of high sensitivity and short detection cycle, it also has some disadvantages.

    Different from the direct isolation of live virus from human body, nucleic acid detection adopts an indirect method, which may cause false negative.

    Anal swab & test tubes

    Anal swab & test tubes

    How to sample for anal swab test

    1. Soak the swab in normal saline, insert it into the anus of 2-3 cm, wipe it from the folds around the anus, or rotate it gently in the anus. And then insert it into the test tube containing normal saline.

    2. For fecal swab culture, all the above operations require the use of sterile equipment and the swab into a sterile test tube.

    anal swab

    Nasal mini-tip flocked swab

    Nasal mini-tip flocked swab

    Nasal mini-tip flocked swab Medical Nasopharyngeal swab

    Nasopharyngeal swabs are a means of detection. Doctors use nasopharyngeal swabs mainly for special bacterial group infection or virus infection in the nasopharynx. They will use sterile pharyngeal swabs to enter the nasopharynx and throat through the nasal cavity and wipe the mucosa. Conduct bacterial culture, virus culture and drug sensitivity test to determine our treatment plan and diagnosis. This examination is a non-invasive examination.

    Product description

    medical nasal nasopharyngeal mini tip flocked swab

    1. EO sterile, individual packing
    2. With molded breakpoint
    3. Ergonomic and anatomic design, easy to use
    4. Perpendicular nylon fibers, increased assay sensitivity

    CM-96000 NASAL SWAB

    CM-96000 NASAL SWAB

    Product Flocking swab for nasal
    Item No CM-96000
    Material Tip: 100% nylon, handle: plastic (ABS)
    Usage Single use for Nasopharyngeal excretion collection
    Size Diameter: 3mm

    Length: 150mm
    Breaking point: 80mm

    Packing 1pc/pouch, 10000pcs/ctn
    sterile, individual packing cm-96000 Packaging picture

    sterile, individual packing cm-96000 Packaging picture

    Nasal mini-tip flocked swab Packaging & Delivery

    Packaging Details
    Individual Sterile Package
    100 pcs/zip pouch
    5000pcs /carton box
    Carton Size: 52*40*30 cm
    G./W.: 6.5kg
    Port
    shenzhen

    Contact us

    • Cell medical (Dongguan) Co., Ltd.
    • E-mail:flockedswabs@gmail.com
    • No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China
    • Web:www.swabmanufacturer.com/
    Iclean nylon flocked swab

    Iclean nylon flocked swab

    Iclean nylon flocked swab

    Product purpose:

    Iclean nylon flocked swab is ideal for bacteriological sample processing, virological cell culture, DFA test, rapid and direct test, enzyme immunoassay test, polymerase chain reaction and molecular diagnosis based test, as well as forensic identification.

    For throat sampling of respiratory viruses such as influenza, swine influenza, avian influenza, hand, foot and mouth.

    Iclean nylon flocked swab,View more

    Iclean nylon flocked swab,View more

    Characteristics of sampling swab products:

    1. It has extraordinary water absorption capacity, which can increase the number of samples collected on its surface from 20% of traditional sampling swabs to 60%.

    2. The release rate of the collected samples is more than 90%, so as to ensure the high reliability of the results.

    3. Different preservation solutions were selected for different specimen types.

    4. The plastic rod has a unique breakable design to facilitate specimen transportation.

    5. Ethylene oxide sterilization, irradiation sterilization, independent packaging.

    Iclean gene sampling series flocking DNA oral test pieces are specially used to improve the amount and quality of genomic DNA in oral test piece cells. Oral cell sample is a good non-invasive collection and detection method instead of blood. Combined with icleanhcy’s series of DNA separation and stabilization kits, it can quickly collect high-purity and complete DNA samples.

    DNA flocking swab is suitable for human and animal use. It has advantages over other DNA samples in design. Its unique sample matrix and rapid release interface are more conducive to efficient cell collection and maximize DNA yield. The test piece has been sterilized with ethylene oxide and passed the detection of human DNA contamination.

    characteristic

    The unique test substrate greatly improves the yield of DNA;

    Adult oral SWAB piece 1 to 10 μ g DNA;

    It is a good alternative to blood collection;

    Both human and animal can use it;

    Simple and fast operation;

    There are two types of single and double disinfection packaging to choose from;

    application;

    Genotype study;

    Veterinary genotyping and diagnosis;

    Parent child and genetic services;

    Forensic and DNA population studies;

    About us

    Cell medical (Dongguan) Co., Ltd. produce flocked swabs, VTM kits, virus transport medium, with multiple automatic production lines.We support custom production, OEM and so on, and develop products with customers.

    Email:flockedswabs@gmail.com

    Disposable virus sampler

    Disposable virus sampler

    Disposable virus sampler

    ♦ Product Name:

    ① General name: disposable sampler

    ② Name: sample collection kit

    ③ Specification and model: cm-93050t, cm-93050rt, cm-93050rtz, cm-96000t, cm-96000ts, cm98000t, cm-98000tl, cm-761tz

    ♦ Package specification: 50 pcs / bag

    ♦ Intended use: disposable virus sampling tube_ Sampling swab_ Flocking swab_ Saliva collector_ Nucleic acid detection sampling kit_ Throat swab – cell medical (Dongguan) Co., Ltd.

    Medical staff use DNA samplers and disposable samplers for sample collection, transportation and storage.

    ♦ Structure composition: swabs and test tubes. Non sterile.

    Disposable virus sampler_cm-93050t

    Disposable virus sampler_cm-93050t

    ♦ Storage conditions and validity period:

    ① Products shall be stored in a clean, hygienic, dry and ventilated environment;

    ② . the temperature is 5 ℃ ~ 35 ℃;

    ③ . relative humidity < 85% RH;

    ④ Product validity: two years

    ♦ usage method:

    ① Before sampling, relevant information shall be marked on the label of the sampling tube.

    ② . use the corresponding sampling flocked swab for sampling.

    ③ After sampling, quickly put the cotton swab into the sampling tube and tighten the tube cover.

    ④ . see the following for specific sampling methods:

    a) Nasal swab: gently insert the sampling head into the nasopalatine part of the nasal canal, stay for a moment, then slowly rotate and exit, put the collected specimen into the sampling tube, and tighten the cover of the sampling tube.

    b) Pharyngeal swab: wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with the sampling head, put the collected samples into the sampling tube, and tighten the cover of the sampling tube.

    c) Sampling of mycoplasma, chlamydia and Ureaplasma samples:

    Male: insert the sampling head into the urethra and rotate about 2cm. Stay for a moment and then exit. Put the collected specimen into the sampling tube and tighten the cover of the sampling tube.

    Female: wipe the mucus from the cervical mouth, insert the sampling head into the cervical tube for 1-2cm for sampling, put the collected sample into the sampling tube, and tighten the cover of the sampling tube.

    ♦ matters needing attention:

    ① . After collecting the sample, the medical staff shall completely insert the disposable sampling cotton swab into the test tube to retain the sample to the greatest extent.

    ② . the collected samples must be fresh and submitted for inspection in time.

    ③ .forbidden to use products with damaged packaging and expiration date to prevent pollution.

    ④ . forbidden to directly contact the collected patient, and forbidden to sample the patient after wetting the swab.

    ⑤ . When collecting samples, medical staff should strictly implement the sampling procedures to ensure that the sampling position is accurate and the sampling intensity is uniform and appropriate, otherwise the sample collection quality will be affected.

    ⑥ The product shall not be used after the expiration date or the product package is damaged.

    Note: this disposable sampler : for in vitro diagnosis and not for human or animal internal and external use.

    If swallowed, it may lead to serious events and cause certain irritation to eyes and skin. If it accidentally splashes into eyes, rinse with clean water.

    ♦ essential information

    Name of manufacturer: cell medical (Dongguan) Co., Ltd

    Domicile: No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China

    E-mail: flockedswabs@gmail.com

    Disposable virus sampling tube kit

    Disposable virus sampling tube kit

    Disposable virus sampling tube kit

    The virus sampling tube consists of swab + sampling tube + virus preservation solution.

    That is, flocked swab, virus sampling tube and virus transport medium.VTM

    Why use a disposable virus sampling tube kit?

    Virus is a very tiny organism, which is composed of nucleic acid and protein shell. Without its own metabolic mechanism, there is no protease system. Therefore, if the virus leaves the host cell, it cannot continue to survive. Such tiny creatures can bring us many diseases, such as RNA, influenza, HIV and H1N1 viruses. Because it has no cellular structure, the virus itself cannot replicate. Instead, it invades the gene into the host cell and replicates a new virus with the help of the latter’s replication system.

    In order to maintain the activity of virus samples and prolong the survival time of virus in samples.

    The sampling swab will be stored and transported in the preservation solution.

    flocked swab_Disposable virus sampling tube kit

    flocked swab_Disposable virus sampling tube kit

    Brief introduction

    1. In the current COVID-19 epidemic situation, virus sample collection is an important link in virus detection.

    2. Disposable virus sampling tube kits can collect, transport, inactivate and store virus samples in specific parts of human body. (not available in the United States).

    So what’s the difference between the non inactivated and inactivated virus sampling tubes?

    about non inactivated and inactivated virus sampling Virus transport medium

    about non inactivated and inactivated virus sampling Virus transport medium

    In short, the non inactivated sampling tube can protect the protein nucleic acid of the virus, so that the sampled virus has its originality, better integrity and higher detection rate. In addition to nucleic acid detection, scientists can also use non inactivated samples in other virus research experiments.

    Advantages:

    ★ easy to operate and use

    ★ add virus stabilizing components to maintain virus activity in a wide temperature range

    And reduce the decomposition rate of virus (non inactivated type)

    1. Low temperature non freezing storage, does not damage the shell of the virus, and is convenient for long-distance transportation.

    2. Applicable to all kinds of swab samples, including oral swab, nasal swab, throat swab, etc.

    ★ it contains virus lysate and virus nucleic acid preservation solution, which can quickly lyse the virus

    Release nucleic acid and stably store nucleic acid (inactivated type)

    The inactivated sampling tube contains cleavage salt, which can destroy the protein of the virus. However, it can protect the nucleic acid of the virus, so that the virus has no infectious activity. The sampling virus in the inactivated sampling tube can prevent the risk of secondary infection and ensure the safety of transportation and testing personnel.

    note

    1. forbidden to directly contact the preservation solution with virus samples.

    2. forbidden to infiltrate the swab with preservation solution before sampling.

    3. only used for the collection, transportation and preservation of virus samples. It shall not be used beyond the expected purpose.

    4. Before using this product, pay attention to the shelf life and whether the packaging is intact.

     

     

     

    Sample collection swab :How much do you know about nucleic acid testing

    Sample collection swab :How much do you know about nucleic acid testing

    Sample collection swab:How much do you know about nucleic acid testing

    The spread of the covid-19  is continuing worldwide. Although the global scientific and medical circles are making every effort to fight the epidemic, it is difficult to predict how the epidemic will develop in the future, and its impact on social development has appeared in many aspects.

    Currently, COVID-19 cases are over 220 million.

    Currently, COVID-19 cases are over 220 million.

    In order to fully control covid-19, the general screening of local areas shall be carried out if necessary.

    After identifying the detection object, how to detect it?

    1、 What specimen to choose?

    According to the recommendations of the American infectious diseases association, the following specimens were used:

    The sensitivity of simple oropharyngeal swab may not be enough, so it is relatively easy to miss diagnosis.

    The sensitivity of alveolar lavage fluid is the highest, but it is obviously not suitable for universal screening.

    Nasopharynx swabs and other operations should also be standardized.

    Improper operation may make the quality of collected samples not high, thus increasing the risk of missed diagnosis.

    2、 Select the time of detection

    It is very important for COVID-19 to measure RNA and choose the right time. One analysis included 7 studies, including 2 unpublished reports, to evaluate the detection efficiency of RT-PCR at different time points after exposure to virus :

    On the day of exposure to the virus, the possibility of detecting viral RNA is almost zero;

    On the fifth day after exposure, that is, the first day after infection, the detection probability was 62%

    About the 8th day after exposure, i.e. the 4th day after infection, the probability of detection was 80%

    On the 21st day after exposure, i.e. the 17th day after infection, the detection probability was 34%

    In conclusion, exposure to COVID-19 may require multiple tests to avoid missed diagnosis. However, it is not recommended to repeat the test within 24 hours of the previous test; The interval between two tests shall be at least 24 hours.

    After 28 days of exposure to the virus, if the infection has not been diagnosed, it is not necessary to continue the detection; At this time, it is almost certain that it is not infected.

    Nucleic acid detection throat swab  sampling process

    The COVID-19 nucleic acid was novel coronavirus, which was collected from sputum or lower respiratory tract by real-time fluorescent RT-PCR. If positive, the new coronavirus could be diagnosed. This is the basis for the diagnosis of new type of pneumonia. In addition to detecting the nucleic acid of the virus, it can also detect whether the virus in the specimen is highly homologous with New Coronavirus. If so, it can be diagnosed as New Coronavirus pneumonia. The virus is highly infectious and has the characteristics of human to human transmission, but the virus is sensitive to temperature and sunlight and ultraviolet rays.

    Sample collection, nasopharyngeal swab sampling

    Sample collection, nasopharyngeal swab sampling

    Sample collection, Oropharyngeal swab sampling

    Sample collection, Oropharyngeal swab sampling

    The flocked swab is the best raw material for sampling swabs.

     

     

    reference

    1,Infectious Diseases Society of America Guidelines on the Diagnosis of COVID-19, updated December 23, 2020. https://www.idsociety.org/practice-guideline/covid-19-guideline-diagnostics/ (Accessed on January 14, 2021).

    2,Kucirka LM, Lauer SA, Laeyendecker O, et al. Variation in False-Negative Rate of Reverse Transcriptase Polymerase Chain Reaction-Based SARS-CoV-2 Tests by Time Since Exposure. Ann Intern Med 2020; 173:262.

     

    COVID-19 sampling tools

    COVID-19 sampling tools help SARS-CoV-2 precise diagnosis!

    COVID-19 sampling tools help SARS-CoV-2 precise diagnosis!

    Cell medical (Dongguan) Co.,Ltd. monthly production of millions of virus sampling swabs and VTM kits helps epidemic prevention and control.

    What are the covid-19 sampling tools?

    Flocked sampling Swabs,VTM Kits,Viral Transport Medium,VIRUS Collection & Transport Kits,Saliva collection kit,Disposable virus sampling tube,Op swabs,Np swabs,etc.

    Why use sampling tools?

    At present, the global New Coronavirus epidemic has reached a critical stage. The most reliable means of rapid diagnosis is still positive for nucleic acid detection. There has been a great breakthrough in the diagnosis of COVID-19, but clinicians, especially the laboratory doctors, still face the following serious problems:

    1. Safety of specimens

    The majority of “angels in white”, especially the laboratory doctors, are always facing the risk of virus infection.

    Although there are various protective equipment, the risk of infection will increase if we can’t ensure the safety of specimens. Therefore, it is very important to ensure virus inactivation and the safety of collected samples.

    2. False negative test results

    The existence of false negative puzzles the clinic and affects the accuracy of diagnosis. The biggest problem is sample collection and preservation. The New Coronavirus infection prevention and control program (Fifth Edition) has been published to collect nasal swabs, throat swabs, sputum, lower respiratory tract secretions, blood and feces. The incorrect way of collecting and storing samples will lead to the degradation of viral RNA and directly lead to the false negative test results.

    Cell medical (Dongguan) Co., Ltd. provides the perfect solution for sampling tools.

    √. Cell medical provides samples of swabs collected from various viral types including New Coronavirus (covid-19) and provides a perfect solution.

    √ .It can quickly inactivate viruses including New Coronavirus, influenza A virus and many other viruses.

    √ .preservation solution: preserve RNA to prevent degradation.Disposable virus sampling tube.

    √ .safety guarantee: vertical design, sampling and preservation in one step to reduce personnel exposure.

    Sampling steps of new virus collection tube

    1) The collection personnel shall be qualified through biosafety training and have corresponding experimental skills. Requirements for protective packaging of sampling personnel: N95 and above protective masks, goggles, one-piece protective clothing, double-layer latex gloves, waterproof boot covers, etc. in case of direct contact with the specimen, the protective equipment shall be replaced in time.

    2) Sample type: pharyngeal swab and nasal swab.

    nasal swab_COVID-19 sampling tools

    nasal swab_COVID-19 sampling tools

    Pharyngeal swab collection method: wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with a plastic rod swab with polypropylene fiber head

    Nasal swab collection method: gently insert a plastic rod swab with polypropylene fiber head into the nasopalatine part of the nasal canal, stay for a moment, and then slowly rotate and exit. Take another plastic rod swab with polypropylene fiber head and collect the other nostril wall in the same way.

    3) Specimen preservation: immerse the swab head into the tube containing virus preservation solution, discard the handle along the folding point of the sampling swab, and tighten the tube cover.

    Notes

    The tube containing virus preservation solution another name isvirus sampling tube.

    Virus preservation solution is also called virus transport medium.VTM.