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COVID-19 Sample Collection and Transport kits

Posted on 2021年9月24日2021年12月21日 by Dr. Lee

Since the outbreak of the new crown, we have received many customer queries about which types of swabs, media and kits can be used for sample collection and transportation of COVID-19.

Cell medical (Dongguan) Co., Ltd. produce flocked swabs, VTM kits, virus transport medium, with multiple automatic production lines.

We support custom production, OEM and so on, and develop products with customers.

Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.

Our VTM kits are manufactured in accordance with CDC and FDA guidelines.

Now let’s learn about sample collection and transport kits.

COVID-19 Sample Collection and Transport kits

How do we collect COVID-19 samples?

Specimen collection requirements

Personal protective equipment (PPE) requirements for sampling personnel: N95 and above protective masks, goggles, one-piece protective clothing, double-layer latex gloves and waterproof boots; In case of contact with the patient’s blood, body fluids, secretions or excreta, replace the outer latex gloves in time.

Specimen type

1. Upper respiratory tract specimens: including pharyngeal swab, nasal swab, nasopharyngeal extract, etc.

2. Lower respiratory tract specimens

3. Stool samples: Patients with diarrhea symptoms need to collect stool samples.

Specimen collection method

Sampling flocked swab_Sample Collection

1. Pharyngeal swab: wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with 2 plastic rod swabs planted with pile at the same time, immerse the swab head into a tube containing 3ml virus preservation solution (isotonic salt solution, tissue culture solution or phosphate buffer can also be used), discard the tail, and tighten the tube cover.

2. Nasal swab: gently insert a plastic rod swab with a flocking nylon faucet into the nasopalatine part of the nasal canal, stay for a moment, and then slowly rotate and exit. Take another plastic rod swab with polypropylene fiber head and collect the other nostril in the same way. Immerse the above two swabs into the same tube containing 3ml sampling solution, discard the tail, and tighten the tube cover.

What kind of Media do you use to transport specimens?

Since the outbreak of COVID-19, the Virus Transport Medium has played a major role. Sample and nasal swabs are used to protect samples in the sample tube with virus preservation, which improves the accuracy of detection. Virus preservation solution is a solution for virus sampling, preservation and detection. What kinds of virus preservation solution are added to the virus sampling tube?

virus transport medium

There are two types of virus preservation transport Media: inactivated and non inactivated.

The following briefly introduces several principles for the selection of virus preservation solution.

Non inactivated virus transport medium:

The non inactivated type does not contain lysate.

On the basis of Hank’s, BSA (bovine serum albumin) amino acids, vitamins and other nutrients required by the virus are added, which can maintain the activity of the virus in a wide temperature range and maintain the originality of the sample to the greatest extent.

For nucleic acid extraction and detection of the virus, virus culture and separation.

Characteristics of non inactivated preservation solution:

COVID-19 Sample Collection and Transport kits Use process

1. It can maintain virus activity for detection or virus isolation;

2. It has no inhibitory effect on subsequent nucleic acid amplification;

3. stored in cold storage(at 2 ℃ ~ 8 ℃ ).

Inactivation virus transport medium:

Inactivation preservation solution can inactivate the virus in the sample by fully mixing the collected sample with virus lysate and virus nucleic acid preservation solution, and effectively ensure the integrity of virus nucleic acid in the sample.

About the preserved viral RNA samples: used in gene detection, enzyme-linked immunosorbent assay (ELISA), PCR detection and so on.

Inactivation preservation solution includes guanidine salt containing and guanidine salt free.

1. Guanidine salt

(1) Instantly cleaved and inactivated to reduce the risk of sampling;

(2) Stored stably at room temperature for 2 weeks;

(3) It can match most nucleic acid extraction reagents.

2. Non refined salt

(1) Inactivated instantaneously to reduce the virus sampling risk, and the virus may recover its infectivity after dilution;

(2) Stored stably at room temperature for 2 weeks;

(3) It can match most nucleic acid extraction reagents.

3. Direct expansion preservation solution

(1)Inactivated instantaneously to reduce the virus sampling risk, and the virus may recover its infectivity after dilution;

(2)Stored stably in cold storage for 24-48 hours;

(3) Directly added to the nucleic acid amplification system as a template;

(4) Match most nucleic acid extraction reagents and nucleic acid release agents.

Sample Universal Viral Transport Medium Kits

Posted on 2021年9月20日2021年12月21日 by Dr. Lee

Sample Universal Viral Transport Medium kits

Cell medical (Dongguan) Co., Ltd. disposable sample universal viral transport medium kits are composed of flocked swabs and test tubes with virus preservation solution (VTM).

The appearance of VTM is clear or red, and there are no precipitates, lumps and other insoluble visible foreign matters.

For collecting, storing and transporting human nasopharyngeal virus samples. Such as COVID-19, mycoplasma, Chlamydia specimens collection, transportation and preservation.

Universal Viral Transport Medium for Viruses

Test principle:

Virus: Composed of a nucleic acid molecule and protein or only protein, and the individual is small and the structure is simple. Because there is no cell structure, the virus cannot replicate itself, but invades its genes into the host cell, using the latter’s replication system to replicate new viruses. After the virus sample is collected, in order to maintain the viability of the virus sample and prolong the survival time of the virus in the sample, the sampling swab will be stored and transported in the transport medium.

Viral transport medium composition and inspection principle:

Viral Transport Medium

1. A combination of multiple antibiotics has broad-spectrum anti-bacterial and anti-fungal effects.

2. As a protein stabilizer, bovine serum albumin (BSA) can form a protective film on the protein shell of the virus, making it difficult to decompose and ensuring the integrity of the virus.

3. The neutral environment constructed by Hank’s buffer helps increase the survival time of the virus and the stability of infection.

4. Phenol red is an acid-base indicator, the discolouration area is 6.6 (yellow)-8.0 (red), and it will be red at 7.2-7.4.

The most stable pH of the outer protein of the virus is 7.2-7.4, which is also the pH range of Hank’s buffer.

If contaminated moulds or bacteria are not inhibited by antibiotics, they will multiply and release carbon dioxide to lower the pH. When the pH value is lower than 7.2-7.4, the stability of the outer protein of the virus will decrease and the decomposition will be accelerated. At this time, the appearance of the sampling solution will change from red to yellow.

What are the main components of Viral Transport Medium(VTM)?

Hank’s solution base, gentamicin, fungal antibiotics, cryoprotectants, biological buffers and amino acids.

On the basis of Hank’s, adding HEPES and other virus stabilizing components can maintain virus activity in a wide temperature range, reduce virus decomposition speed and improve the positive rate of virus isolation.

How to use a universal viral transport medium?

Please read the instructions carefully before use.

Working conditions: room temperature

1. Take out the virus preservation solution.

2. Use a sampling swab for sampling.

3. Insert the swab into the tube containing virus preservation solution, break the tail, leave it in the preservation solution tube, cover the tube cover.

4. Store it at 2-4 ℃ and use it within 48 hours.

If it needs to be stored for more than 48 hours, please store it in the refrigerator at – 20 ℃.

VTM swab kit COVID-19: diagnostic vector transport medium.

Our VTM product features and advantages.

1. A virus transport medium (VTM) in line with the recommendations of who and CDC.

2. The product is safe, environment-friendly and non-toxic.

3. The universal viral transport medium adds special virus stabilizing components to maintain the structural stability of virus core protein and outer membrane protein for a long time.

4. It is especially suitable for influenza virus and other samples sampled through upper respiratory tract swabs.

5. Compatible with other detection reagents.

6. The reagent itself has a colour indication function, and obvious color change will occur when the product deteriorates, so as to avoid misuse caused by improper preservation.

COVID-19 Pooled Sample Testing:Improve sampling rate and reduce cost

Posted on 2021年9月18日2021年12月21日 by Dr. Lee

COVID-19 Pooled Sample Testing:Improve sampling rate and reduce cost.

With the advent of autumn and winter, the possibility of a new outbreak of COVID-19 is increasing.

COVID-19’s large-scale community screening is the main means to cope with the recent outbreak of small-scale epidemics at home and abroad.

At present, New Coronavirus’s mixed sample nucleic acid detection has become a routine method to effectively enhance the screening and detection of nucleic acid in community population.

The pooled sample test of covid-19 increase the sampling rate and meet the growing needs of low-risk populations.

Virus transport medium,disposable virus sampling tube

What is a Pooled Sample Test?

“mixed detection”, that is, nucleic acid test of Pooled Sample.

On the one hand,it is a method to sample several people, such as 3 or 5 or even 10, and then put them into the same virus sampling tube.

On the other hand,pooled Sample means combining the same type of specimen from several people and conducting one NAAT laboratory test on the combined pool of specimens to detect SARS-CoV-2.

What is the significance of nucleic acid test in Pooled Sample?

COVID-19 Pooled Sample Testing Advantages

Because a virus sampling tube only puts one person’s sample, the cost will be very high, and the detection time is long, which is not suitable for large-scale nucleic acid detection.

In principle, the single sample sampling mode is only applicable to high-risk areas and key populations.

In order to improve sampling efficiency, shorten sampling time and reduce sampling cost. Multi Country selection of Pooled sample test scheme.

For example, when 5-in-1 mixed sampling test is used, the result is negative, which means that all five people are negative.

On the contrary, once positive or weak positive is found, it will be traced immediately.

Re collect single tube swabs for review, and then determine which of the 5 people is positive.

Therefore, the cost of 5-in-1 mixed mining is relatively low.

This also caused the 5-in-1 mixed sampling mode to be five times faster than the previous mode of using a virus sampling tube for a human sample.

One of the biggest disadvantages of sample merging is the decrease of associated test sensitivity.

Analyzing multiple samples in a single reaction effectively improves the flux, but it also dilutes a single sample and may reduce the weak positive signal from outside the detection limit.

In short, pooled sample testing is mainly used for large-scale screening to check asymptomatic coronavirus infected persons. It is suitable for large-scale testing projects such as schools, factories, units and urban areas. It has the advantages of efficient screening and resource saving.

Although reduced detection sensitivity is acceptable for screening services in asymptomatic populations.

However, When providing diagnosis for high-risk or symptomatic patients, we must give priority to the accuracy of the results.

The special preservation tube for Pooled Sample test uses medical grade PP as raw material, unique structural design and manufacturing process to prevent liquid leakage. And has the characteristics of high temperature resistance, high pressure resistance and high-speed centrifugation resistance.

Cell medical (Dongguan) Co., Ltd. produces virus sampling tubes of various specifications, both inactivated and non inactivated, disposable virus sampling tubes, pooled sample sampling tubes.
such as 1ml, 2ml, 3ml, 5ml, 10ml and other specifications.

Located in Guangdong Province, in China,with complete qualifications.

Contact information required.E-mail:flockedswabs@gmail.com

China universal viral transport medium factory manufacturers

Posted on 2021年9月16日2022年3月30日 by Dr. Lee

China universal viral transport medium factory and manufacturers

Product specification

VTM KIT_Virus sampling tube

[common name]: disposable virus sampling tube, viral transport medium kits,universal viral transport media.

[specification and model]: 3ml / piece, 100 pieces / bag

[main structure and performance]

It usually consists of swabs and/or preservation tubes containing viral transport medium.

[scope of application]

For sample collection, transportation and storage.

[action principle]

After sterilizing the sampling tube, add virus stable components such as Hank’s base solution, which can maintain the activity of virus in a wide temperature range, reduce the decomposition speed of virus and improve the positive rate of virus isolation.

Description

Viral Transport Medium (VTM) allows the safe transfer of viruses, chlamydia and mycoplasma for further research, including conventional cell culture methods, diagnostic tests, and molecular biology techniques.

Commercially prepared viral transport media are available in a screw cap plastic tube containing buffered proteins (serum, albumin or gelatin) and antibiotics.

Viral transport medium composition

Our viral transport media contains Hanks Balanced Salt Solution (HBSS) with Calcium and Magnesium and contains heat-inactivated Fetal Bovine Serum, Gentamycin and Amphotericin B.

The composition and the manufacturing of the Viral Transport Medium follow the WHO and the CDC recommendations.

[contraindications, precautions, warnings and suggestive instructions]

1. After sampling with a virus sampling tube, the sampler shall submit it for inspection as soon as possible.

At the same time, the transportation personnel shall immediately transport the virus samples at a low temperature of 2-8 ℃.

The results showed that the virus samples could be stored at 2-8 ℃ for 48 hours; But for long-term storage, below – 20 ℃ and – 70 ℃.

If the sampler does not immediately transport at low temperature or store at the specified temperature after sampling. It will affect the positive rate of virus sample separation in the later stage.

2. prohibited to use it for the sampling of bacterial samples. The antibiotics contained in the sampling solution can inhibit bacteria.

3. forbidden to directly contact the collected patient, and forbidden to sample the patient after wetting the swab.

4. The sampling personnel shall collect virus samples in strict accordance with the sampling procedures to ensure the accuracy of sampling location. At the same time, the sampling intensity is uniform and appropriate, otherwise, it will affect the positive rate of virus isolation.

5. It is forbidden to use the expired or damaged product package.

6. The experimenter shall test the samples in a laboratory meeting the safety level.

Factory profile

Virus transport medium factories, Cell medical is a professional manufacturer of sampling swabs and disposable virus sampling tubes (viral transport medium).

The factory is located in Dongguan, China, with a daily production capacity of more than 1 million sets, aseptic filling technology and aseptic swab production technology.

China universal viral transport medium factory and manufacturers_Cell medical (Dongguan) Co., Ltd.

China VTM tube factory GMP factory_Cell medical (Dongguan) Co., Ltd.

Cell medical (Dongguan) Co., Ltd., vtm kit factories, vtm swab factory, produce flocked swabs, VTM kits, virus transport medium, universal viral transport media, with multiple automatic production lines.

We support custom production, OEM and so on, and develop products with customers.

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Email:flockedswabs@gmail.com

Comparison of advantages of various testing methods of COVID-19

Posted on 2021年9月14日2021年9月15日 by Dr. Lee

advantages of various testing methods

The detection methods of COVID-19 mainly include nucleic acid detection, antibody detection and antigen detection. Due to the low detection rate of antigen, the SARS-CoV-2 detection mainly focuses on antibody and nucleic acid detection. Nucleic acid detection has the characteristics of early diagnosis, high sensitivity and specificity; However, antibody detection is convenient and rapid, which can be used as a supplementary means of nucleic acid diagnosis. Due to the limitations of “false positive” and “false negative” antibody detection, it is not suitable for general population screening such as returning to work, and school, nor for epidemiological investigation in low epidemic areas.

NAT Detection process

The detection procedure needs to go through five steps: sampling, sample retention, preservation, nucleic acid extraction and computer detection.

First, collect samples according to the instructions of the kit. The sample types include pharyngeal swab, nasal swab, sputum, bronchial lavage fluid, alveolar lavage fluid, etc.

Because RNA is easy to degrade, flocked swabs without RNase and storage tubes without RNase are used when collecting samples.

After obtaining the patient’s sample, it needs to be tested as soon as possible. If it cannot be tested immediately, it needs to be packaged at low temperature and sent to a special testing institution for testing.

After receiving the sample, the testing institution shall extract the nucleic acid from the sample. The nucleic acid extraction reagent shall use the nucleic acid extraction kit specified in the approved product manual.

Finally, fluorescence PCR nucleic acid detection, that is, machine detection. It takes 70-80 minutes to amplify the extract by fluorescence PCR.

Sample collection, oropharyngeal swab or nasopharyngeal swab

NAT Detection process Nucleic Acid Testing

Antibody detection

After the virus invades the human body, the human body will produce corresponding specific antibodies for defense.

According to different molecular structures and antigen specificity, scientists divide immunoglobulins into five categories: IgG, IgA, IgM, IgD and IgE.

After the antigen enters the human body for the first time, plasma cells secrete antibodies after a certain incubation period. IgM first appeared, but the antibody had short maintenance time, low concentration and low affinity. It lasted for several days to several weeks in the blood. It was a diagnostic index of acute infection; When IgM is about to disappear, the content of IgG reaches the peak. IgG appeared late, but its concentration was high, its maintenance time was long and its affinity was high. The positive serum IgG indicates the middle and late stage of infection or previous infection. But the antibody content increases rapidly in the short term, which may be several times to dozens of times higher than the original antibody content. It is mainly IgG, which lasts a long time in the body, and IgM rarely increases.

In this epidemic, the researchers studied patients with covid-19 and found that after the virus invades the human body, IgM antibody takes about 5-7 days to produce and IgG antibody takes 10-15 days to produce.

Antibody detection kits-advantages of various testing methods

Comparison of advantages and disadvantages of two different detection methods

Compared with the serological detection of antibodies, Nucleic Acid Testing can detect patients in the window stage.

Early detection of infected persons is the “gold standard” for COVID-19 detection, but it requires higher detection equipment or platform.

The high sensitivity RT-PCR instrument is expensive, and the laboratory cleanliness and operator requirements are higher. In addition, nucleic acid detection takes a long time. Considering the sample transportation and sample backlog, the results can be reported as soon as 24 hours.

Compared with nucleic acid detection, blood samples detected by antibody serology are easier to obtain. If the RNA of the virus detected by nucleic acid is the direct evidence of the existence of the virus, the antibody detection is the antibody stimulated in the patient’s blood, which is indirect evidence and has a suggestive effect on the clinic.

When nucleic acid detection is negative, adding IgM and IgG antibody detection can make up for the shortcoming that nucleic acid detection is easy to cause missed diagnosis.

About us

Cell medical (Dongguan) Co., Ltd., a professional VTM kits and swabs manufacturer, has many years of experience in medical application molding and flocked treatment heads and swabs.

Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.Certificate:FDA,CE,EU,Free Sale,Export license based on (GMP).

We produce flocked swabs, VTM kits, Virus transport medium, with multiple automatic production lines.We support custom production, OEM and so on, and develop products with customers.

E-mail：flockedswabs@gmail.com
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Disposable virus sampling tube kit

Posted on 2021年9月11日2021年9月11日 by Dr. Lee

Disposable virus sampling tube kit

The virus sampling tube consists of swab + sampling tube + virus preservation solution.

That is, flocked swab, virus sampling tube and virus transport medium.VTM

Why use a disposable virus sampling tube kit？

Virus is a very tiny organism, which is composed of nucleic acid and protein shell. Without its own metabolic mechanism, there is no protease system. Therefore, if the virus leaves the host cell, it cannot continue to survive. Such tiny creatures can bring us many diseases, such as RNA, influenza, HIV and H1N1 viruses. Because it has no cellular structure, the virus itself cannot replicate. Instead, it invades the gene into the host cell and replicates a new virus with the help of the latter’s replication system.

In order to maintain the activity of virus samples and prolong the survival time of virus in samples.

The sampling swab will be stored and transported in the preservation solution.

flocked swab_Disposable virus sampling tube kit

Brief introduction

1. In the current COVID-19 epidemic situation, virus sample collection is an important link in virus detection.

2. Disposable virus sampling tube kits can collect, transport, inactivate and store virus samples in specific parts of human body. (not available in the United States).

So what’s the difference between the non inactivated and inactivated virus sampling tubes?

about non inactivated and inactivated virus sampling Virus transport medium

In short, the non inactivated sampling tube can protect the protein nucleic acid of the virus, so that the sampled virus has its originality, better integrity and higher detection rate. In addition to nucleic acid detection, scientists can also use non inactivated samples in other virus research experiments.

Advantages:

★ easy to operate and use

★ add virus stabilizing components to maintain virus activity in a wide temperature range

And reduce the decomposition rate of virus (non inactivated type)

1. Low temperature non freezing storage, does not damage the shell of the virus, and is convenient for long-distance transportation.

2. Applicable to all kinds of swab samples, including oral swab, nasal swab, throat swab, etc.

★ it contains virus lysate and virus nucleic acid preservation solution, which can quickly lyse the virus

Release nucleic acid and stably store nucleic acid (inactivated type)

The inactivated sampling tube contains cleavage salt, which can destroy the protein of the virus. However, it can protect the nucleic acid of the virus, so that the virus has no infectious activity. The sampling virus in the inactivated sampling tube can prevent the risk of secondary infection and ensure the safety of transportation and testing personnel.

note

1. forbidden to directly contact the preservation solution with virus samples.

2. forbidden to infiltrate the swab with preservation solution before sampling.

3. only used for the collection, transportation and preservation of virus samples. It shall not be used beyond the expected purpose.

4. Before using this product, pay attention to the shelf life and whether the packaging is intact.