Sample Universal Viral Transport Medium kits
Cell medical (Dongguan) Co., Ltd. disposable sample universal viral transport medium kits are composed of flocked swabs and test tubes with virus preservation solution (VTM).
The appearance of VTM is clear or red, and there are no precipitates, lumps and other insoluble visible foreign matters.
For collecting, storing and transporting human nasopharyngeal virus samples. Such as COVID-19, mycoplasma, Chlamydia specimens collection, transportation and preservation.
Universal Viral Transport Medium for Viruses
Test principle:
Virus: Composed of a nucleic acid molecule and protein or only protein, and the individual is small and the structure is simple. Because there is no cell structure, the virus cannot replicate itself, but invades its genes into the host cell, using the latter’s replication system to replicate new viruses. After the virus sample is collected, in order to maintain the viability of the virus sample and prolong the survival time of the virus in the sample, the sampling swab will be stored and transported in the transport medium.
Viral transport medium composition and inspection principle:
1. A combination of multiple antibiotics has broad-spectrum anti-bacterial and anti-fungal effects.
2. As a protein stabilizer, bovine serum albumin (BSA) can form a protective film on the protein shell of the virus, making it difficult to decompose and ensuring the integrity of the virus.
3. The neutral environment constructed by Hank’s buffer helps increase the survival time of the virus and the stability of infection.
4. Phenol red is an acid-base indicator, the discolouration area is 6.6 (yellow)-8.0 (red), and it will be red at 7.2-7.4.
The most stable pH of the outer protein of the virus is 7.2-7.4, which is also the pH range of Hank’s buffer.
If contaminated moulds or bacteria are not inhibited by antibiotics, they will multiply and release carbon dioxide to lower the pH. When the pH value is lower than 7.2-7.4, the stability of the outer protein of the virus will decrease and the decomposition will be accelerated. At this time, the appearance of the sampling solution will change from red to yellow.
What are the main components of Viral Transport Medium(VTM)?
Hank’s solution base, gentamicin, fungal antibiotics, cryoprotectants, biological buffers and amino acids.
On the basis of Hank’s, adding HEPES and other virus stabilizing components can maintain virus activity in a wide temperature range, reduce virus decomposition speed and improve the positive rate of virus isolation.
How to use a universal viral transport medium?
Please read the instructions carefully before use.
Working conditions: room temperature
1. Take out the virus preservation solution.
2. Use a sampling swab for sampling.
3. Insert the swab into the tube containing virus preservation solution, break the tail, leave it in the preservation solution tube, cover the tube cover.
4. Store it at 2-4 ℃ and use it within 48 hours.
If it needs to be stored for more than 48 hours, please store it in the refrigerator at – 20 ℃.
Our VTM product features and advantages.
1. A virus transport medium (VTM) in line with the recommendations of who and CDC.
2. The product is safe, environment-friendly and non-toxic.
3. The universal viral transport medium adds special virus stabilizing components to maintain the structural stability of virus core protein and outer membrane protein for a long time.
4. It is especially suitable for influenza virus and other samples sampled through upper respiratory tract swabs.
5. Compatible with other detection reagents.
6. The reagent itself has a colour indication function, and obvious color change will occur when the product deteriorates, so as to avoid misuse caused by improper preservation.