Pcr test nasal swab and an antigen test difference for covid 19

Posted on 2021年9月18日2022年3月22日 by Dr. Lee

What is COVID-19?COVID-19 is an epidemic disease caused by SARS-CoV-2. An acute respiratory infectious disease broke out at the end of 2019. So far, WHO has been making the latest tracking report on the new pneumonia on the Internet every day.

What are the current methods of COVID-19 test?

Virus detection includes two types: nucleic acid amplification test (NAAT) (PCR test) and antigen test.

Antibody tests (also known as serological tests) can prove whether you have been infected in the past.

What is the COVID-19 PCR test?

PCR (polymerase chain reaction): similar to the natural replication process of DNA, its specificity depends on oligonucleotide primers complementary to both ends of the target sequence.

Basic principle: the basic principle of PCR technology is similar to the natural replication process of DNA. Its specificity mainly depends on the oligonucleotide primers complementary to both ends of the target sequence. It consists of three basic reaction steps: denaturation, renaturation and extension.

PCR test sample collection method include:

If the sample contains a virus, the researchers will extract its RNA.

Why use the PCR test ?

PCR nucleic acid examination is a direct detection of COVID-19 RNA in human body by sampling from the nasal cavity. The structure of virus is composed of DNA or RNA combined with external lipid protective capsule. Therefore, direct detection of virus DNA itself is the most accurate, fastest and effective new detection method. PCR nucleic acid test can detect infection immediately after the human body obtains the virus, and the accuracy is much higher than antibody test.

ABOUT PCR TEST_Pcr test nasal or Throat swab sampling

How does the PCR test nasal swab work?

Nasopharynx swab is to wipe the secretion of the patient’s nasal cavity or pharynx. And then make the secretion into smear for examination and bacterial culture to clarify what bacterial infection is in the patient’s nasal cavity and pharynx.

Nasal swab sampling procedure

1. Keep the patient’s head still and remove the secretions on the middle surface of the anterior nostril;

2. Gently and slowly insert the swab through the nasal cavity to the nasopharynx;

3. After encountering resistance, it reaches the posterior nasopharynx and stays for several seconds to absorb secretions;

4. Gently rotate and take out the swab and place it in the transport medium;

5. For the swab used for virological test, immerse the swab head into the virus delivery medium, discard the tail, and tighten the tube cover;

If it is necessary to collect from both nostrils, the sampler should use the same swab.

How does the antigen test work?

Rapid antigen detection can play an important role in guiding patient management, public health prevention and control decision-making and covid-19 monitoring.

It is especially valuable for grass-roots areas that may hinder clinical diagnosis and control due to lack of nucleic acid detection capacity or long detection time.

According to WHO, high specificity based on antigen detection indicates that the positive results indicate new crown virus infection.

For patients within 9 days of onset, sampling personnel can use antigen detection as a diagnostic test.

Especially for the staff of medical institutions, inpatients and visitors in the epidemic area.

When nucleic acid detection cannot be effectively implemented, antigen detection can be used as an important screening method.

Rapid antigen test sampling procedure.

Rapid antigen test sampling process

Generally speaking, nucleic acid detection: it has high requirements for laboratory environment, detection personnel and instruments. The detection has high sensitivity and good specificity.Generally, we can get the results in 2-3 hours.

Antigen detection: it has low requirements for the laboratory. For early screening and early diagnosis. It is suitable for large-scale screening in grass-roots hospitals. we can obtain the results within 15 minutes at the fastest.

At present, PCR test nasal swab is widely used, and the sensitivity and specificity of antibody / antigen detection reagents are still limited.

Each has its own emphasis and cannot replace each other. We should combine multiple virus detection methods.

It can effectively shorten the detection window period and improve the positive detection rate.

Respiratory Specimen Collections

Posted on 2021年9月15日2022年3月22日 by Dr. Lee

Respiratory Specimen Collections

Today, with the increasingly severe international COVID-19 epidemic, the correct collection of respiratory samples has become an important prerequisite for ensuring the accuracy of nucleic acid detection results.

However, how to correctly collect respiratory samples? Cell medical (Dongguan) Co., Ltd. summarizes as follows:

Why should respiratory tract samples be collected standardized and correctly?

1. Clear diagnosis;

2. Observe the condition and treatment effect;

3. Guide the formulation of treatment measures;

4. Sample quality directly determines the reliability of test results.

What are the types of respiratory samples?

1. Upper respiratory tract samples (including swabs, nasal specimens, nasopharynx extracts, pharyngeal gargle and nasal lotion, etc.);

2. Lower respiratory tract samples (such as sputum, tracheal aspirate, lung lotion, etc.);

When is the best time to collect respiratory samples?

Respiratory tract samples were collected as soon as possible after symptoms appeared;

Collect as much as possible within 72 hours of the patient’s fever;

Try to collect before the use of antiviral drugs;

If possible, collect multiple times in multiple days;

What sampling tools are needed to collect respiratory tract samples?

1. Virus sampling kits, including flocked swab, virus sampling tube, virus transport medium, etc;

2. Refrigerated transport box;

3. Personal protective equipment: protective mask (N95), protective glasses.

How to collect respiratory tract samples?

Oropharyngeal swab sampling process:

Oropharyngeal swab sampling pictures

1. The sampler first let the patient sit down, then let the patient tilt his head back and open his mouth.

2. Press the front 2 / 3 of the patient’s tongue with a tongue depressor, and wipe the posterior pharyngeal wall and bilateral tonsils with a nylon flocked swab for 3-5 times to avoid touching the tongue.

3. Take out the rear sampling tube, break the plastic handle at the contact part of the hand, soak the swab into the sampling solution, and tighten the tube cover.

Nasopharyngeal swab:

Note : do not use too much force, but try to be as deep as possible, usually more than 5cm, stay for several seconds to absorb secretions, gently rotate and take out the swab, place the sampling tube, break the plastic ellipse at the contact part of the hand, and soak the test piece into the sampling solution, Tighten the pipe cover.

Respiratory Specimen Collections preservation requirements

Those that can be detected within 24 hours can be stored at 4 ℃ and frozen at – 70 ℃ for more than 24 hours.

Swab test | Disposable virus sampling swab

Posted on 2021年9月15日2021年9月15日 by Dr. Lee

Swab test | Disposable virus sampling swab

Disposable virus sampling swab, I believe you are no stranger in the past two years. Because most of the detection tools used for nucleic acid test are disposable sampling swabs. Usually, we see most nasal swabs and pharyngeal swabs. But disposable virus swabs are not only useful for nucleic acid testing. What else can a disposable sampling swab do? Let’s get to know.

What can a disposable virus swab be used for?

In addition to nucleic acid detection, scientists can also use disposable sampling swabs for microbial collection. They are usually used for virus detection and research in hospitals or laboratories. Researchers use disposable sampling swabs to detect biological samples in natural cavities of human or animals. Such as throat, nose, mouth, vagina and so on. It is necessary to collect oral epidermal cells and nasal virus samples with a sampling swab, store the cells and samples in the sampling tube and transfer them to the laboratory for examination.

Oropharyngeal swab sampling

Operation (use) method of disposable sampling swab:

Take pharyngeal swab as an example:

① Tear open the packaging bag of the disposable sampling swab and take out the disposable sampling swab from the bag;

Note: please do not let the disposable sampling swab touch the place outside the throat to avoid pollution.

② Put a disposable sampling swab into the mouth and quickly wipe the palatal arch and throat (or lesion) on both sides

③ Take out the swab, put it into the disposable virus sampling tube, break the disposable sampling swab at the broken place and discard the tail;

④ Tighten the sampling tube, put the virus sampling tube into the biosafety bag and submit it for inspection in time.

Note: please do not shake the virus sampling tube excessively to avoid dilution and loss of samples. After sampling, do not touch other objects with a disposable sampling swab.

How to carry out COVID-19 nasal swab test?

The doctor shall wear protective clothing, mask, gloves and mask to let the patient take a proper position.

Gently insert the flocked sampling swab into the nose, rotate it for three times, take it out and put it into the sample tube with protective solution, and screw the cover. Then label it, write the date, and send it to the laboratory for nucleic acid test of nasopharyngeal swab.

What is a COVID-19 swab test?

Swab test, namely nucleic acid test. The substance test by nucleic acid is the nucleic acid of virus. Nucleic acid detection is to collect the presence of viruses in the respiratory tract, blood or feces of patients, and determine whether they are infected by COVID-19. Therefore, once detected as nucleic acid “positive”, it can prove that there is a virus in the patient’s body.

SARS-CoV-2 nucleic acid detection process.

The usual sample types include throat swabs, nasal swabs, sputum, bronchoalveolar lavage fluid, alveolar lavage fluid and so on.

The sampling personnel shall conduct PCR detection as soon as possible after obtaining patient samples.

After the testing institution receives the sample, the laboratory personnel shall extract nucleic acid from the sample. And the nucleic acid extraction reagent shall use the nucleic acid extraction kit specified in the approved product manual.

Disposable virus sampling swab material.

At present, because of its stable and reliable performance and excellent absorption and elution capacity, nylon flocked swabs are widely used in COVID-19 sampling.

Swab test result

Swab testing for novel coronavirus pneumonia usually takes 4-6 hours to produce results, while rapid examination takes 2-3 hours. However, the reagents used in each hospital are different, so the time of results is also different.

COVID-19 positive results：Maybe infected.After 24 hours of self isolation, the positive patients need to conduct swab test again.

If the two results are positive, the next step is to improve CRP, blood routine, chest CT and other tests to determine whether COVID-19.

When COVID-19 nucleic acid test is negative once, it should be combined with the clinical symptoms and epidemiological contact history of patients. Because a COVID-19 nucleic acid swab test is negative, it can not completely exclude the infection of COVID-19.

Iclean nylon flocked swab

Posted on 2021年9月11日2021年12月21日 by Dr. Lee

Iclean nylon flocked swab

Product purpose:

Iclean nylon flocked swab is ideal for bacteriological sample processing, virological cell culture, DFA test, rapid and direct test, enzyme immunoassay test, polymerase chain reaction and molecular diagnosis based test, as well as forensic identification.

For throat sampling of respiratory viruses such as influenza, swine influenza, avian influenza, hand, foot and mouth.

Iclean nylon flocked swab,View more

Characteristics of sampling swab products:

1. It has extraordinary water absorption capacity, which can increase the number of samples collected on its surface from 20% of traditional sampling swabs to 60%.

2. The release rate of the collected samples is more than 90%, so as to ensure the high reliability of the results.

3. Different preservation solutions were selected for different specimen types.

4. The plastic rod has a unique breakable design to facilitate specimen transportation.

5. Ethylene oxide sterilization, irradiation sterilization, independent packaging.

Iclean gene sampling series flocking DNA oral test pieces are specially used to improve the amount and quality of genomic DNA in oral test piece cells. Oral cell sample is a good non-invasive collection and detection method instead of blood. Combined with icleanhcy’s series of DNA separation and stabilization kits, it can quickly collect high-purity and complete DNA samples.

DNA flocking swab is suitable for human and animal use. It has advantages over other DNA samples in design. Its unique sample matrix and rapid release interface are more conducive to efficient cell collection and maximize DNA yield. The test piece has been sterilized with ethylene oxide and passed the detection of human DNA contamination.

characteristic

The unique test substrate greatly improves the yield of DNA;

Adult oral SWAB piece 1 to 10 μ g DNA;

It is a good alternative to blood collection;

Both human and animal can use it;

Simple and fast operation;

There are two types of single and double disinfection packaging to choose from;

application;

Genotype study;

Veterinary genotyping and diagnosis;

Parent child and genetic services;

Forensic and DNA population studies;

About us

Cell medical (Dongguan) Co., Ltd. produce flocked swabs, VTM kits, virus transport medium, with multiple automatic production lines.We support custom production, OEM and so on, and develop products with customers.

Email:flockedswabs@gmail.com

Disposable virus sampler

Posted on 2021年9月11日2022年3月22日 by Dr. Lee

Disposable virus sampler

♦ Product Name:

① General name: disposable sampler

② Name: sample collection kit

③ Specification and model: cm-93050t, cm-93050rt, cm-93050rtz, cm-96000t, cm-96000ts, cm98000t, cm-98000tl, cm-761tz

♦ Package specification: 50 pcs / bag

♦ Intended use: disposable virus sampling tube_ Sampling swab_ Flocking swab_ Saliva collector_ Nucleic acid detection sampling kit_ Throat swab – cell medical (Dongguan) Co., Ltd.

Medical staff use DNA samplers and disposable samplers for sample collection, transportation and storage.

♦ Structure composition: swabs and test tubes. Non sterile.

Disposable virus sampler_cm-93050t

♦ Storage conditions and validity period:

① Products shall be stored in a clean, hygienic, dry and ventilated environment;

② . the temperature is 5 ℃ ~ 35 ℃;

③ . relative humidity ＜ 85% RH;

④ Product validity: two years

♦ usage method:

① Before sampling, relevant information shall be marked on the label of the sampling tube.

② . use the corresponding sampling flocked swab for sampling.

③ After sampling, quickly put the cotton swab into the sampling tube and tighten the tube cover.

④ . see the following for specific sampling methods:

a) Nasal swab: gently insert the sampling head into the nasopalatine part of the nasal canal, stay for a moment, then slowly rotate and exit, put the collected specimen into the sampling tube, and tighten the cover of the sampling tube.

b) Pharyngeal swab: wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with the sampling head, put the collected samples into the sampling tube, and tighten the cover of the sampling tube.

c) Sampling of mycoplasma, chlamydia and Ureaplasma samples:

Male: insert the sampling head into the urethra and rotate about 2cm. Stay for a moment and then exit. Put the collected specimen into the sampling tube and tighten the cover of the sampling tube.

Female: wipe the mucus from the cervical mouth, insert the sampling head into the cervical tube for 1-2cm for sampling, put the collected sample into the sampling tube, and tighten the cover of the sampling tube.

♦ matters needing attention:

① . After collecting the sample, the medical staff shall completely insert the disposable sampling cotton swab into the test tube to retain the sample to the greatest extent.

② . the collected samples must be fresh and submitted for inspection in time.

③ .forbidden to use products with damaged packaging and expiration date to prevent pollution.

④ . forbidden to directly contact the collected patient, and forbidden to sample the patient after wetting the swab.

⑤ . When collecting samples, medical staff should strictly implement the sampling procedures to ensure that the sampling position is accurate and the sampling intensity is uniform and appropriate, otherwise the sample collection quality will be affected.

⑥ The product shall not be used after the expiration date or the product package is damaged.

Note: this disposable sampler : for in vitro diagnosis and not for human or animal internal and external use.

If swallowed, it may lead to serious events and cause certain irritation to eyes and skin. If it accidentally splashes into eyes, rinse with clean water.

♦ essential information

Name of manufacturer: cell medical (Dongguan) Co., Ltd

Domicile: No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China

E-mail： flockedswabs@gmail.com

Disposable virus sampling tube kit

Posted on 2021年9月11日2021年9月11日 by Dr. Lee

Disposable virus sampling tube kit

The virus sampling tube consists of swab + sampling tube + virus preservation solution.

That is, flocked swab, virus sampling tube and virus transport medium.VTM

Why use a disposable virus sampling tube kit？

Virus is a very tiny organism, which is composed of nucleic acid and protein shell. Without its own metabolic mechanism, there is no protease system. Therefore, if the virus leaves the host cell, it cannot continue to survive. Such tiny creatures can bring us many diseases, such as RNA, influenza, HIV and H1N1 viruses. Because it has no cellular structure, the virus itself cannot replicate. Instead, it invades the gene into the host cell and replicates a new virus with the help of the latter’s replication system.

In order to maintain the activity of virus samples and prolong the survival time of virus in samples.

The sampling swab will be stored and transported in the preservation solution.

flocked swab_Disposable virus sampling tube kit

Brief introduction

1. In the current COVID-19 epidemic situation, virus sample collection is an important link in virus detection.

2. Disposable virus sampling tube kits can collect, transport, inactivate and store virus samples in specific parts of human body. (not available in the United States).

So what’s the difference between the non inactivated and inactivated virus sampling tubes?

about non inactivated and inactivated virus sampling Virus transport medium

In short, the non inactivated sampling tube can protect the protein nucleic acid of the virus, so that the sampled virus has its originality, better integrity and higher detection rate. In addition to nucleic acid detection, scientists can also use non inactivated samples in other virus research experiments.

Advantages:

★ easy to operate and use

★ add virus stabilizing components to maintain virus activity in a wide temperature range

And reduce the decomposition rate of virus (non inactivated type)

1. Low temperature non freezing storage, does not damage the shell of the virus, and is convenient for long-distance transportation.

2. Applicable to all kinds of swab samples, including oral swab, nasal swab, throat swab, etc.

★ it contains virus lysate and virus nucleic acid preservation solution, which can quickly lyse the virus

Release nucleic acid and stably store nucleic acid (inactivated type)

The inactivated sampling tube contains cleavage salt, which can destroy the protein of the virus. However, it can protect the nucleic acid of the virus, so that the virus has no infectious activity. The sampling virus in the inactivated sampling tube can prevent the risk of secondary infection and ensure the safety of transportation and testing personnel.

note

1. forbidden to directly contact the preservation solution with virus samples.

2. forbidden to infiltrate the swab with preservation solution before sampling.

3. only used for the collection, transportation and preservation of virus samples. It shall not be used beyond the expected purpose.

4. Before using this product, pay attention to the shelf life and whether the packaging is intact.

Sample collection swab :How much do you know about nucleic acid testing

Posted on 2021年9月10日2022年3月22日 by Dr. Lee

Sample collection swab:How much do you know about nucleic acid testing

The spread of the covid-19 is continuing worldwide. Although the global scientific and medical circles are making every effort to fight the epidemic, it is difficult to predict how the epidemic will develop in the future, and its impact on social development has appeared in many aspects.

Currently, COVID-19 cases are over 220 million.

In order to fully control covid-19, the general screening of local areas shall be carried out if necessary.

After identifying the detection object, how to detect it?

1、 What specimen to choose?

According to the recommendations of the American infectious diseases association, the following specimens were used:

Nasopharyngeal swab
Middle turbinate swab
Anterior nostril swab
saliva
Anterior nostril + oropharyngeal swab
Alveolar lavage fluid
Anal swab

The sensitivity of simple oropharyngeal swab may not be enough, so it is relatively easy to miss diagnosis.

The sensitivity of alveolar lavage fluid is the highest, but it is obviously not suitable for universal screening.

Nasopharynx swabs and other operations should also be standardized.

Improper operation may make the quality of collected samples not high, thus increasing the risk of missed diagnosis.

2、 Select the time of detection

It is very important for COVID-19 to measure RNA and choose the right time. One analysis included 7 studies, including 2 unpublished reports, to evaluate the detection efficiency of RT-PCR at different time points after exposure to virus :

On the day of exposure to the virus, the possibility of detecting viral RNA is almost zero;

On the fifth day after exposure, that is, the first day after infection, the detection probability was 62%

About the 8th day after exposure, i.e. the 4th day after infection, the probability of detection was 80%

On the 21st day after exposure, i.e. the 17th day after infection, the detection probability was 34%

In conclusion, exposure to COVID-19 may require multiple tests to avoid missed diagnosis. However, it is not recommended to repeat the test within 24 hours of the previous test; The interval between two tests shall be at least 24 hours.

After 28 days of exposure to the virus, if the infection has not been diagnosed, it is not necessary to continue the detection; At this time, it is almost certain that it is not infected.

Nucleic acid detection throat swab sampling process

The COVID-19 nucleic acid was novel coronavirus, which was collected from sputum or lower respiratory tract by real-time fluorescent RT-PCR. If positive, the new coronavirus could be diagnosed. This is the basis for the diagnosis of new type of pneumonia. In addition to detecting the nucleic acid of the virus, it can also detect whether the virus in the specimen is highly homologous with New Coronavirus. If so, it can be diagnosed as New Coronavirus pneumonia. The virus is highly infectious and has the characteristics of human to human transmission, but the virus is sensitive to temperature and sunlight and ultraviolet rays.

Sample collection, nasopharyngeal swab sampling

Sample collection, Oropharyngeal swab sampling

The flocked swab is the best raw material for sampling swabs.

reference

1，Infectious Diseases Society of America Guidelines on the Diagnosis of COVID-19, updated December 23, 2020. https://www.idsociety.org/practice-guideline/covid-19-guideline-diagnostics/ (Accessed on January 14, 2021).

2，Kucirka LM, Lauer SA, Laeyendecker O, et al. Variation in False-Negative Rate of Reverse Transcriptase Polymerase Chain Reaction-Based SARS-CoV-2 Tests by Time Since Exposure. Ann Intern Med 2020; 173:262.

What is genetic testing?

Posted on 2021年9月9日2021年9月9日 by Dr. Lee

What is genetic testing?

Definition of gene

What we usually call genes is a collection of genes in human cells, called the genome.

Every cell in our body has a complete genome. Inherited from parents and passed on to children.

Genes make people different from animals, make families different from families, and brothers and sisters are similar but different.

These subtle differences often do not exceed 0.5% of genes.

But determine different appearance, personality, genetic characteristics, and our adaptation to the external environment.

Why genetic testing?

It must be noted that various human traits and diseases are the result of the joint action of genes and environment .

Genes only play a partial role in traits, and whether gene expression is also greatly affected by the internal and external environment.

Therefore, gene test reports can not be directly used as the basis for clinical treatment, and it is not advisable to judge the role of genes without individual environment.

On this premise, the gene test report still has a certain reference significance for understanding various characteristics of the human body.

Understand that they carry hereditary pathogenic genes

We can know whether we carry pathogenic genes through genetic testing. For example, people with a family history of genetic diseases, cancer and hypertension. According to the test results, adjust dietary health care and living habits to avoid the occurrence of diseases. If both pregnant couples carry the pathogenic gene of recessive disease at the same time. They can pay attention and conduct further investigation when preparing for delivery.

Select drugs correctly to avoid drug waste and adverse drug reactions

Due to genetic differences, individuals will respond differently to drugs. When taking the same kind of drugs, some people will feel very effective, while others will not only be ineffective, but also have toxic and side effects. When some people use certain drugs, they may also have drug allergy. Through gene testing, we can know everyone’s gene situation, which may play a guiding role in drug use to a certain extent. Again, the drug test report can not be directly used in clinic. The drug selection and use in the treatment of diseases need to follow the doctor’s advice.

Provide scientific health management

Many adverse environmental factors and bad living habits will make the human body more prone to diseases. People can understand the occurrence tendency of individuals in different diseases through genetic testing, and make corresponding life adjustment or intervention in order to reduce the risk and delay the occurrence of diseases.

What is the principle of gene testing?

To understand the principle of gene detection, we need to understand the following questions:

Reasons for gene testing

Each person’s human nucleus has 23 pairs of chromosomes, which are composed of DNA and ribosomal proteins. All chromosomes have 3.16 billion base pairs.

When the same base pair position changes (this situation is generally called single nucleotide polymorphism, or SNP), which has a certain impact on human genetics and health, this SNP is the locus in the gene report. At present, there are nearly more than 100 million known or named SNPs. Most of the differences between human DNA are SNP differences, which is about 0.5% of the total base pairs of human DNA. A large part of gene detection is based on SNP.

Samples for genetic testing

Normal human samples required for gene testing include saliva sampling and blood sampling. For different examination items, excreta sampling and cell slice sampling are also included.

Methods of gene testing

There are several ways to measure related base pair differences (SNPs): PCR, chip, exon, and genome-wide. The number of SNPs detected is dozens, hundreds of thousands, tens of thousands, and all SNPs.

Disposable DNA sampling flocked swab

Oral DNA sampling swab_flocked swab cm-98000

1.Specially designed for micro DNA samples at the crime scene, especially for micro samples such as sweat, semen, blood, dandruff, oral cells, etc.

2. It can quickly absorb a small amount of samples and has high release aging rate.

3. The front end is sharp enough to extract cells from the victim or suspect’s fingernails.

4. The swab has a breakpoint, which is convenient for the automatic extraction workstation to extract samples.

5. The whole swab is packed in a transparent plastic tube to avoid biological pollution.

Who can do genetic testing?

① People with family disease history;

② People with bad living habits;

③ People exposed to chemical pollution, heavy metal pollution, nuclear pollution and toxic and harmful substances for a long time;

④ Friends who pay attention to health;

Hello!

When many diseases are diagnosed, it’s too late. Do a good job in disease prevention to make us healthy for a longer time!

Nasal swab pcr test

Posted on 2021年9月7日2021年12月21日 by Dr. Lee

Nasal swab pcr test COVID-19 Antigen Rapid Test Kit

Detection of COVID-19 by nasal swab PCR is an accurate and reliable method. Positive detection means you may have SARS-CoV-2. Negative detection means you may not have SARS-CoV-2 when testing.

In order to ensure your safety, if you have symptoms of COVID-19. Or people who have been exposed to the virus and tested positive should be tested.

What is the symptom of COVID-19?

If you have the following symptoms,if you have the following symptoms, please buy covid-19 antigen rapid test kit immediately or go to a nearby hospital for PCR test.

1.Fever;

2.General fatigue;

3.cough;

4.Dyspnea or even respiratory failure；

5.Nausea;

6.vomiting;

7.abdominal pain;

8.diarrhea;

Symptoms of New Coronavirus pneumonia are not necessarily typical, and some patients may not have clinical manifestations at the early stage of New Coronavirus infection. Then clinical manifestations will appear, that is, “asymptomatic infection” in incubation period.

In other words, try to avoid activities in places with crowd gathering, closed space and poor ventilation.

What is nasal swab PCR test？

Polymerase chain reaction (PCR) uses a piece of DNA as a template. Then, with the participation of DNA polymerase and nucleotide substrate, the DNA was amplified to a sufficient number for structural and functional analysis.

Scientists use PCR technology to amplify a small amount of RNA in the sample into deoxyribonucleic acid (DNA) and replicate until COVID-19(if present) is detected.

What should we pay attention to before doing nucleic acid test？

1.Try not to eat 2 hours before sampling to avoid vomiting.

2. Do not smoke, drink, drink water or chew gum 30 minutes before sampling.

3. The tester needs to wear a mask correctly and remove the mask before testing;

4. Wear it immediately after testing, and prepare a spare mask for easy replacement at any time after pollution.

5.When you feel sour and itchy in your nose or sneeze, you can immediately cover it with a paper towel or elbow.

Before collecting nasopharyngeal swabs and before being tested, the collection personnel shall be informed whether they have relevant past medical history. For example, history of nasal surgery, history of deviation of nasal septum, blood diseases, throat diseases, etc

How long will we know the pcr test results？

The test results of sampling in the morning are generally available from 18:00 to 19:00.

The detection results of afternoon sampling can generally be queried around 22:00.

What’s the difference between the PCR and antigen tests for COVID-19?

Polymerase chain reaction (PCR): PCR is the most reliable and accurate method to detect active infection. PCR tests usually give results in a few hours.

Antigen detection: detect protein fragments on the surface of covid-19. Antigen detection usually takes only 15 to 30 minutes.

How can we prevent COVID-19?

Firstly, keep good personal hygiene habits, wear masks when going out, wash hands frequently, clean the room, and wipe the floor and other furniture with disinfectant. Second, disinfect your hands with 75% alcohol every time you go out and go home, reduce the number of times you go out, and wear qualified masks. And the diet should also be light, do a good job of physical exercise and enhance personal physique.

Most importantly, if our conditions permit, we should vaccinate and conduct multiple nucleic acid tests.

If our Nasal swab pcr test results are positive,what should we do?

If our test results are positive, don’t be afraid. We should wear masks and go to the nearby Hospital for follow-up in time.

Repeat the nucleic acid test.Don’t be afraid and hide the test results. Timely medical treatment is the best choice.

Cell medical (Dongguan) Co., Ltd. is a comprehensive high-tech biotechnology company and an OEM factory of flocking swabs in China.We support custom production,our factory produces flocked swabs, pharyngeal swabs, nasal swabs, oral swabs,Rapid Antigen Test Kit, virus sampling tubes, transportation media, sample preservation solution and nucleic acid extraction kit.

Welcome to leave a message and consult us!

What is a sterile swab？

Posted on 2021年9月7日2022年3月22日 by Dr. Lee

Sterile swab is the full name of disposable sterile sampling swab. The sterile sampling swab does not contain or other live microorganisms. Common sterilization methods include autoclave, EOS (ethylene oxide gas) and γ-ray irradiation. In medicine, we usually use sterile swabs for biological sample collection. Such as bacterial sample processing, COVID-19, nasal swab sampling, throat swab sampling, oral swab sampling, virological cell culture and so on are ideal ways.

Swabs are sterile to maintain the integrity of the sample. Usually, there is a resealable sterile container or bag to preserve the integrity during processing and transportation to the laboratory. Many different people use sterile cotton swabs, including medical personnel, food manufacturers and crime scene investigators.

Sterile swabs can be used to collect samples. The material, size and shape of the sterile swab head may be different, such as round or pointed. Traditional swab may be made of cotton fiber, polyester or calcium alginate. Collection and publishing volumes are large and may be corrupted. Traditional swab laboratory staff also need to use scissors to cut, which is too troublesome and time-consuming.Our new flocked disposable sampling cotton swab greatly reduces the time cost and has the advantage of being easier to use than traditional swabs.

The main advantages of our sterile flocked swab are as follows.

1. It has extraordinary water absorption capacity and can increase the number of samples collected on the watch from 20% of the traditional sampling swab to 60%.

2. The release rate of collected samples exceeds 90%, which ensures the high reliability of the results.

3. Select different preservation solutions for different types of samples.

4. The plastic rod has a unique fragile design, which is convenient for sample transportation.

5. Ethylene oxide sterilization, irradiation sterilization and independent packaging.

Sampling operation method.

1.Nasopharyngeal swab sampling.

Nasopharyngeal Sampling diagram

①Insert a sterile swab into the nostril of the patient,reaching the surface of the posterior nasopharynx.

②Swab over the surface of the posterior nasopharynx. Hold the swab in the nosefor15-30s and rotate it 3 times.

③Withdraw the sterile swab from the nasal cavity.

④Put virus sampling tube vertically.

⑤At the breaking point, disconnect the swab, discard the tail, and completely place the swab into the tube.

⑥Tighten the tube cap, mark it, put it into the biosafety bag and seal it.

2.Oropharyngeal swab sampling

Oropharyngeal Sampling diagram

① Keep the head slightly tilted, the mouth open, and the pharyngeal tonsils on both sides exposed.

②The sampling personnel shall wipe the root of the tongue with a swab. Wipe the pharyngeal tonsils on both sides of the subject back and forth with a strong force for at least 3 times, and then wipe the posterior pharyngeal wall up and down for at least 3 times.

③Put the sampled swab into the virus sampling tube, break the swab rod at the mouth of the tube cover and completely put it into the tube.

④Tighten the tube cap, mark it, put it into the biosafety bag and seal it.

⑤Submit for inspection in time.

Anal swab sampling

Anal Sampling diagram

①The patient is in the knee chest position or lateral position.

② Gently insert a sterile cotton swab into the anus for 3cm-5cm, and then gently rotate and pull it out.

③Immediately put it into a 15ml outer screw cap sampling tube with 3ml-5ml virus preservation solution.

④Fold off the tail of the cotton swab, tighten the sealing cover, and then submit it for inspection in time.

⑤In order to avoid embarrassment of the examinee, anal swab sampling is generally conducted in a more private place, and the operation requirements of anal swab sampling are more strict. Generally, there will be no obvious discomfort after anal swab sampling.