Tag Archives: nasopharynx Sampling

Flocked Dacron Swabs

Flocked Dacron Swabs

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Flocked Dacron Swabs

Manufacture Sell Nasopharyngeal Nasal Oral Medical Sterile Flocked Collection Testing dacron swab.

The flocked sampling swabs usually apply asterile dacron or rayon swab with a flexible, plastic shaft that can be easily cut off after thesampling of specimens and put into thetransportation storage tube for storage. Thematerial from the flocked swabs is dispersed into the liquid medium.

Which is better, Tradition swab or flocked swab?

Dacron swab and PBS are acceptable alternatives to flocked swab and viral transport media for SARS-CoV-2 Nasopharyngeal flocked swabs placed in viral transport media (VTM) are the preferred collection methodology for respiratory virus testing.

Looking for more?Flocked swabs definition Here.

Flocked Dacron Swabs-Nasopharyngeal Swab

Flocked Dacron Swabs-Nasopharyngeal Swab

Specification
Material
nylon fibers+medical grade ABS stick
Length
150mm
Breakpoint Length from tip
80mm or 30mm
Handle dia.
1.8-2.5mm
Flocked tip
25mm
OEM/ODM
Available
Sample time
3days
Delivery time
20days
Certificate
CE&ISO13485

1. Sampling is more comfortable and convenient.

Our company uses the existing technology “spraying technology” to vertically attach millions of nylon flocked micro fibers to the head of medical grade handle through the flocking process of electrostatic charge. The function of vertical nylon fiber is similar to that of soft brush. Using this soft brush in the sampling process makes the user feel no foreign matter. At the same time, the flocking swabs used in different parts adopt different breaking points, and the rod adopts different thickness to make the softness different.

For example, nasal swab, its upper end of the rod is thinner and softer, and its breaking point is longer.

Sterile Flocked Nylon Swab with 80mm Breakpoint.

2. Flocking swab samples release more.

The capillary action between nylon fiber bundles promotes the strong hydraulic absorption of liquid samples. The sample remains on the surface to make elution easier. In the traditional cotton swab, most of the samples collected are still captured in the fiber matrix of the head, which is not easy to release or the release amount is small. Flocked swabs have been shown to release up to 95% of the sample, compared with 25% for standard fiber pointed swabs. If the sample size is particularly small, the use of flocking swabs is particularly important.

3. Flocking swab has biocompatibility and is an ideal choice for PCR.

In order to limit the damage to the sample and improve the accuracy of detection, disposable sampling swab is made of better quality nylon flocking swab.

This makes flocking swabs an ideal choice for PCR Swab Test. Therefore, for scientists using PCR technology, the new flocking swab is PCR compatible and does not contain RNase and DNase.

How are flocked swabs used for specimen collection swab?

Our flocked swabs feature perpendicular nylon fibers that optimize specimencollection and elution into transport media. The swabs also feature a moldedbreakpoint that allows you to safely and easily break off the swabstick, and severalbreakpoint options are available for different tubes.

Packaging & Delivery

Selling Units:
Single item
Single package size:
16X1.5X0.5 cm
Single gross weight:
0.005 kg
Package Type:
100pcs/bag,10000pcs/ctn,ctn size:60*40*48cm,10kg/ctn.
Lead Time:
Quantity(Pieces) 1 – 10000 >10000
Est. Time(days) 15 To be negotiated
How to do a Nasopharyngeal Swab

How to do a Nasopharyngeal Swab

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How to do a Nasopharyngeal Swab?

Nasopharyngeal Flocked Swab

Nasopharyngeal Flocked Swab

Nucleic acid detection is to collect Nasopharyngeal swabs, sputum or lower respiratory tract specimens, real-time fluorescence RT-PCR detection of New Coronavirus nucleic acid, if positive, can be diagnosed with New Coronavirus infection, which is the basis for the diagnosis of new type of pneumonia. In addition to detecting the nucleic acid of the virus, it can also detect whether the virus in the specimen is highly homologous with New Coronavirus. If so, it can be diagnosed as New Coronavirus pneumonia. The virus is highly infectious and has the characteristics of human to human transmission, but the virus is sensitive to temperature and sunlight and ultraviolet rays.

Nasopharyngeal swab price：Welcome to email:flockedswabs@gmail.com

Nucleic acid detection and collection method:

(nucleic acid detection nasal swab)

1. Ask the patient to keep his head still and remove the secretions on the surface of the anterior nostril. The collector can stand on the side of the collector to reduce occupational exposure caused by coughing and sneezing and relieve the collector’s pressure.

2. Measure the distance from nostril to ear root with a swab and mark it by hand.

3. Gently insert the swab into the nostril in the direction perpendicular to the nose (face) through the nasal cavity, with a sense of touching the wall until the finger touches the nose, keep the swab in the nose for 15-30 seconds, and then rotate it gently for 3 times.

4. Put the swab into the virus delivery medium, break the swab rod and place it completely in the tube.

5. Tighten the pipe cover, mark it, put it in a plastic bag and seal it.

6. Send the samples for examination in time

swab test nasal swab

swab test nasal swab

1. With the aid of tongue depressor, the collector opens his mouth and makes an “ah” sound. The palate is lifted up to expose the posterior pharyngeal wall. The swab crosses the root of the tongue to reach the pharyngeal stenosis lesion, and quickly wipe the palatal arch, pharyngeal and tonsil secretions on both sides.

2. The nasopharynx swab was put into the virus delivery medium, and the auxiliary tube cover broke the swab rod to make it completely placed in the tube.

3. Tighten the pipe cover, mark it, put it in a plastic bag and seal it.

4. Send the samples for examination in time.

Nasal Swab |nasopharyngeal swab sampling

Nasal Swab |nasopharyngeal swab sampling

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Nasal Swab |nasopharyngeal swab sampling .

People who have done nasal swab testing must be deeply impressed by the taste. The normal length of nasal swab is about 15cm, and it is almost 2 / 3 of the depth when collecting. Does the depth of this direct attack on the soul make your heart question: is our nose so deep?

In fact, the nasal cavity is far from the “superficial” nostrils we see.

Continue to go deep into your nostrils, and you will find that it is suddenly bright and unique.

Schematic diagram of nasopharyngeal sampling

Schematic diagram of nasopharyngeal sampling

Nucleic acid sampling is an important part of anti epidemic work.

It is very important to standardize the operation of sampling technology. It is necessary to standardize various sampling operations in order to provide a strong guarantee for the accurate report of subsequent nucleic acid detection.

How to correctly carry out nasopharyngeal swab sampling?

Nasopharyngeal swab collection method

Material preparation.

75% alcohol, wet towel, test tube rack, collection tube, hand disinfectant, nasopharyngeal swab, biosafety bag, collection bar code.

Operation preparation.

1.Prepare medical protective mask (N95), face screen / goggles, protective clothing and latex gloves.

2.Check the validity period and tightness of the acquisition tube; the virus preservation solution for discoloration and turbidity;  the validity period and packaging of nasopharyngeal swab.

Check the collected information.

1.Ask about recent nasal bleeding, obstruction, trauma, etc.

2.Drive the collection tube on the test tube rack.

3.Hand hygiene.

4.Sampling person: takes the seat, tilts his head back later, and pulls the mask to his lips.

5.Estimated acquisition depth (acquisition depth reference): generally half of the distance from the tip of the nose to the earlobe.

6.The operator stands on the side of the subject, and the swab handle is inserted into one side of the nostril in a direction parallel to the upper jaw.

A sense of resistance indicates that it reaches the nasopharynx; Rotate for one week, stay for 15 seconds, slowly take out the swab and insert it into the specimen preservation solution;

The head of the swab shall not touch hands and other objects, the swab shall be broken, the tail shall be discarded, and the cover of the sampling tube shall be tightened.

7.Hand hygiene.

8.Wipe the tube cover with an alcohol wet towel and conduct hand hygiene again.

9.Put the collection tube into the biosafety bag, seal the bag mouth, and put it into the second biosafety bag in the same way.

Nasal Swab |nasopharyngeal swab sampling

Nasal Swab |nasopharyngeal swab sampling

About flocked swab specimens

Nasopharyngeal sampling swabs are composed of nylon short fiber pile head and medical grade ABS plastic rod.

The nylon short fiber acts like a soft brush, which can effectively improve the collection of cellular material;

the capillary movement between the fibers forms a strong hydraulic pressure, thus ingesting the liquid sample;

at the same time, the sample clings to the surface of the swab and is easy to wash off.

Cell medical (Dongguan) Co., Ltd. Sterile flocked swabs collect and elute samples more effectively than any other disposable swabs on the market.

The high-quality fiber and swab physical manufacturing process produce sterile flocking swabs, which is an ideal choice for rapid diagnostic testing.

The rapid release of whole cells and the ability to better collect cells make these swabs the perfect specimen collection for any laboratory or research center.

The ideal collection device must be able to absorb and release samples and maintain their activity.

This is why flocking cotton swabs innovate sample collection equipment.

Sterile flocked swabs collect and retain sample vitality, provide better sample yield, and allow rapid release of samples into liquid media.

Attention

1.The insertion direction of the swab is perpendicular to the face of the subject, not along the nostril.

2.When there is resistance when the swab enters, it shall retreat slightly. Under the condition that the subject tolerates it, readjust the angle before continuing to insert, and do not insert violently.

 

COVID-19 Swab Test

COVID-19 Swab Test |One Minute to Learn about Nucleic Acid Test

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COVID-19 Swab Test |One Minute to Learn about Nucleic Acid Test

So far, the number of confirmed cases of the global epidemic has exceeded 237 million, followed by the recurrence of the epidemic. We must do a good job in COVID-19 testing and strive to defeat the virus at an early date.

1. Why a nucleic acid test? What does it test for?

Nucleic acid swab detection is the direct evidence to find the existence of virus. The novel coronavirus pneumonia virus nucleic acid test positive, indicating that the presence of virus in the subjects, may be infectious.

If the patient has fever, respiratory symptoms or other clinical features of NCP, the patient can be diagnosed as NCP when combined with positive nucleic acid test; if there are no clinical symptoms, the nucleic acid test result is positive, can be judged as asymptomatic infection.

Novel coronavirus pneumonia is a diagnostic standard for new crown pneumonia.

2. Nasopharyngeal swab test and oropharyngeal swab test, which test method is better?

As we all know, nucleic acid detection generally adopts pharyngeal swab detection. Pharyngeal swab testing includes nasopharyngeal swab testing and oropharyngeal swab testing. The difference between the two lies in the different sampling positions, but there is no essential difference. Most medical institutions use oropharyngeal swab detection, because this detection method has less discomfort and is more convenient for sample collection.

3 .what are the precautions before and after COVID-19 Swab Test?

Before testing;

Get your ID card ready before you go out!

Wear masks correctly without fasting;

Try to avoid eating in the first 2 hours to avoid vomiting and other discomfort;

Try not to drink water, drink, smoke, drink or chew gum in the first 30 minutes;

When sampling, reduce swallowing action, swallow less saliva and don’t clear throat;

We should inform the testing personnel in advance whether they have a history of nasal surgery, bending of nasal septum, blood diseases, throat diseases or taking anticoagulants;

Before testing, remove the mask after the medical personnel give a signal, wear it immediately after sampling, and prepare a spare mask for easy replacement at any time after pollution.

Testing;

Consciously queue up and keep a distance of more than one meter;

When collecting oropharyngeal swabs, tilt your head back, relax as much as possible, breathe deeply, and open your mouth to make an “ah” sound, which is helpful to complete the sampling quickly;

The nose may be itchy during the collection of nasopharyngeal swabs. If you sneeze, cover your mouth and nose with paper towels or elbows immediately;

During the detection, try not to touch the detection platform and other articles, and do not touch the protective clothing of the collection personnel.

After detection

Leave the site immediately after sampling, and do not spit or throw garbage at the collection point and surrounding areas;

The inspectors shall pay attention to hand hygiene before and after the test. They can wipe their hands with hands-free disinfectant, hands-free alcohol, disinfection cotton, etc.

4 How can nucleic acid detection alleviate the discomfort of sampling?

Nasopharynx swab sampling and oropharyngeal swab sampling will make the sampled person feel slightly uncomfortable, which can be relieved after a short rest. Therefore, during the sampling process, the sampled person shall cooperate with the sampling personnel as much as possible, relax as much as possible, and do not over move the head to avoid scratching the mucosa with the sampling swab.

Pcr test nasal swab and an antigen test difference for covid 19

Pcr test nasal swab and an antigen test difference for covid 19

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Pcr test nasal swab and an antigen test difference for covid 19

What is COVID-19?COVID-19 is an epidemic disease caused by SARS-CoV-2. An acute respiratory infectious disease broke out at the end of 2019. So far, WHO has been making the latest tracking report on the new pneumonia on the Internet every day.

What are the current methods of COVID-19 test?

Virus detection includes two types: nucleic acid amplification test (NAAT) (PCR test) and antigen test.

Antibody tests (also known as serological tests) can prove whether you have been infected in the past.

What is the COVID-19 PCR test?

PCR (polymerase chain reaction): similar to the natural replication process of DNA, its specificity depends on oligonucleotide primers complementary to both ends of the target sequence.

Basic principle: the basic principle of PCR technology is similar to the natural replication process of DNA. Its specificity mainly depends on the oligonucleotide primers complementary to both ends of the target sequence. It consists of three basic reaction steps: denaturation, renaturation and extension.

PCR test sample collection method include:

If the sample contains a virus, the researchers will extract its RNA.

Why use the PCR test ?

PCR nucleic acid examination is a direct detection of COVID-19 RNA in human body by sampling from the nasal cavity. The structure of virus is composed of DNA or RNA combined with external lipid protective capsule. Therefore, direct detection of virus DNA itself is the most accurate, fastest and effective new detection method. PCR nucleic acid test can detect infection immediately after the human body obtains the virus, and the accuracy is much higher than antibody test.

ABOUT PCR TEST_Pcr test nasal or Throat swab sampling

ABOUT PCR TEST_Pcr test nasal or Throat swab sampling

How does the PCR test nasal swab work?

Nasopharynx swab is to wipe the secretion of the patient’s nasal cavity or pharynx. And then make the secretion into smear for examination and bacterial culture to clarify what bacterial infection is in the patient’s nasal cavity and pharynx.

Nasal swab sampling procedure

1. Keep the patient’s head still and remove the secretions on the middle surface of the anterior nostril;

2. Gently and slowly insert the swab through the nasal cavity to the nasopharynx;

3. After encountering resistance, it reaches the posterior nasopharynx and stays for several seconds to absorb secretions;

4. Gently rotate and take out the swab and place it in the transport medium;

5. For the swab used for virological test, immerse the swab head into the virus delivery medium, discard the tail, and tighten the tube cover;

If it is necessary to collect from both nostrils, the sampler should use the same swab.

How does the antigen test work?

Rapid antigen detection can play an important role in guiding patient management, public health prevention and control decision-making and covid-19 monitoring.

It is especially valuable for grass-roots areas that may hinder clinical diagnosis and control due to lack of nucleic acid detection capacity or long detection time.

According to WHO, high specificity based on antigen detection indicates that the positive results indicate new crown virus infection.

For patients within 9 days of onset, sampling personnel can use antigen detection as a diagnostic test.

Especially for the staff of medical institutions, inpatients and visitors in the epidemic area.

When nucleic acid detection cannot be effectively implemented, antigen detection can be used as an important screening method.

Rapid antigen test sampling procedure.

Rapid antigen test sampling process

Rapid antigen test sampling process

 

Generally speaking, nucleic acid detection: it has high requirements for laboratory environment, detection personnel and instruments. The detection has high sensitivity and good specificity.Generally, we can get the results in 2-3 hours.

Antigen detection: it has low requirements for the laboratory. For early screening and early diagnosis. It is suitable for large-scale screening in grass-roots hospitals. we can obtain the results within 15 minutes at the fastest.

At present, PCR test nasal swab is widely used, and the sensitivity and specificity of antibody / antigen detection reagents are still limited. 

Each has its own emphasis and cannot replace each other. We should combine multiple virus detection methods.

It can effectively shorten the detection window period and improve the positive detection rate.

 

Mini flocked nose swab china factory and manufacturers

Mini flocked nose swab china factory and manufacturers

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Mini flocked nose swab china factory and manufacturersCell medical (Dongguan) Co., Ltd.,  flocked swab adopts vertical nylon fiber, which has stable and reliable performance and good adsorption and elution ability. Flocked swabs optimize sample collection and elution into the transmission medium. The swab also has a molded breakpoint that allows you to safely and easily disconnect the cotton swab. Our company provides multiple breakpoint options for different pipes.We can customize swab mold, swab packaging, sterile packaging and swab OEM services.

Mini flocked nose swab Specifications

Mini flocked nasal swab

Mini flocked nasal swab

 

  • Swab Length: 145mm±5mm
  • Flocked Tip Length: 8mm
  • Swab Tip Diameter: 8mm±0.2mm
  • Breakpoint: 80/90mm
  • Packing: Individual Sterile Package
  • Certificate: CE/FDA/ISO
  • OEM/ODM: Support
  • GMP Factory: YES
  • Supplying Ability: 500,0000pcs/ day

 

Application: Nasopharyngeal Sampling Swab

Mateiral: 100% Medical Grade Nylon

Sterilization: EO

Certificate: ISO13485, CE, FDA, SGS, MSDS, TDS.

What’s the use of a flocked nose swab？

Nasal swab detection is a kind of nucleic acid detection, which is used to detect the presence of viral nucleic acid in the body. So as to determine whether the human body infected with COVID-19.

Nasal swab detection requires collecting cells deep in the throat of the tested person and placing them in a Petri dish for observation. Because New Coronavirus is mainly transmitted through the respiratory tract,if the human body is infected with COVID-19, there will be a lot of viruses in the throat. Therefore, it is a more accurate way to detect the existence of COVID-19 by detecting the cells in the throat.

What is the method of mini nasal swab collection?

① Please keep the patient’s head still and remove the secretions on the surface of the anterior nostril;

② use a flocked swab or other tools to the distance from the nostril to the root of the ear and mark it with your fingers;

③ insert the swab gently and slowly through the nasal cavity;

④ swab until the finger mark or stay for several seconds after encountering resistance to absorb secretions; (generally stay for 15-30 seconds, and then rotate gently for 3 times);

⑤ gently rotate the swab and put it into the virus sampling tube (transport medium); Break the swab tail rod along the breaking point and place it completely in the tube. Tighten the pipe cover.

⑥ If you need to collect from two nostrils, you should use another swab.

⑦ Send to the inspection room for inspection as soon as possible.

⑧ Note the information of the collected person to avoid confusion.

Mini flocked nasal swab sampling display

Mini flocked nasal swab sampling display

China universal viral transport medium

China universal viral transport medium factory manufacturers

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China universal viral transport medium factory and manufacturers

Product specification

VTM KIT_Virus sampling tube

VTM KIT_Virus sampling tube

[common name]: disposable virus sampling tube, viral transport medium kits,universal viral transport media.

[specification and model]: 3ml / piece, 100 pieces / bag

[main structure and performance]

It usually consists of swabs and/or preservation tubes containing viral transport medium.

[scope of application]

For sample collection, transportation and storage.

[action principle]

After sterilizing the sampling tube, add virus stable components such as Hank’s base solution, which can maintain the activity of virus in a wide temperature range, reduce the decomposition speed of virus and improve the positive rate of virus isolation.

 

Description

Viral Transport Medium (VTM) allows the safe transfer of viruses, chlamydia and mycoplasma for further research, including conventional cell culture methods, diagnostic tests, and molecular biology techniques.

Commercially prepared viral transport media are available in a screw cap plastic tube containing buffered proteins (serum, albumin or gelatin) and antibiotics.

Viral transport medium composition

Our viral transport media contains Hanks Balanced Salt Solution (HBSS) with Calcium and Magnesium and contains heat-inactivated Fetal Bovine Serum, Gentamycin and Amphotericin B.

The composition and the manufacturing of the Viral Transport Medium follow the WHO and the CDC recommendations.

[contraindications, precautions, warnings and suggestive instructions]

1. After sampling with a virus sampling tube, the sampler shall submit it for inspection as soon as possible.

At the same time, the transportation personnel shall immediately transport the virus samples at a low temperature of 2-8 ℃.

The results showed that the virus samples could be stored at 2-8 ℃ for 48 hours; But for long-term storage,  below – 20 ℃ and – 70 ℃.

If the sampler does not immediately transport at low temperature or store at the specified temperature after sampling. It will affect the positive rate of virus sample separation in the later stage.

2.  prohibited to use it for the sampling of bacterial samples. The antibiotics contained in the sampling solution can inhibit bacteria.

3. forbidden to directly contact the collected patient, and forbidden to sample the patient after wetting the swab.

4. The sampling personnel shall collect virus samples in strict accordance with the sampling procedures to ensure the accuracy of sampling location. At the same time, the sampling intensity is uniform and appropriate, otherwise, it will affect the positive rate of virus isolation.

5. It is forbidden to use the expired or damaged product package.

6. The experimenter shall test the samples in a laboratory meeting the safety level.

Factory profile

Virus transport medium factories, Cell medical is a professional manufacturer of sampling swabs and disposable virus sampling tubes (viral transport medium).

The factory is located in Dongguan, China, with a daily production capacity of more than 1 million sets, aseptic filling technology and aseptic swab production technology.

China universal viral transport medium factory and manufacturers_Cell medical (Dongguan) Co. ,Ltd.

China universal viral transport medium factory and manufacturers_Cell medical (Dongguan) Co., Ltd.

China VTM tube factory GMP factory_Cell medical (Dongguan) Co. ,Ltd.

China VTM tube factory GMP factory_Cell medical (Dongguan) Co., Ltd.

Cell medical (Dongguan) Co., Ltd., vtm kit factories, vtm swab factory, produce flocked swabs, VTM kits, virus transport medium, universal viral transport media, with multiple automatic production lines.

We support custom production, OEM and so on, and develop products with customers.

Get Latest Price

Email:flockedswabs@gmail.com

Is anal swab nucleic acid test more accurate than nasopharyngeal swab?

Is anal swab nucleic acid test more accurate than nasopharyngeal swab?

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Is anal swab nucleic acid test more accurate than nasopharyngeal swab?

Nucleic acid detection:generally carried out by sampling pharyngeal swabs in the nasal cavity and oral cavity.

Now began to increase the anal swab test, we are also curious about this test method. So, is anal swab nucleic acid detection more accurate than nasopharyngeal swab? How to sample for anal swab test?

Is the nucleic acid detection of anal swab more accurate than that of nasopharyngeal swab?

In the screening of New Coronavirus pneumonia, we mainly used the oropharyngeal swab nucleic acid test. Because the reason is convenient and quick, suitable for large-scale screening. The accuracy of nasopharynx swab for new crown nucleic acid detection is higher than that of oropharyngeal swab, but the human body feels uncomfortable.

In last year’s test, researchers found that many infected people had negative pharyngeal nucleic acid.But their anal swabs were still positive for longer.

The combination of the two in nucleic acid detection can improve the detection rate and reduce the proportion of missed diagnosis.

However, anal swab sampling is inconvenient and not suitable for large-scale screening and testing in the community.

For some asymptomatic or mild infections, the condition recovers quickly after infection. And the pharyngeal nucleic acid may not be detected in 3 to 5 days.

The researchers found that the duration of nucleic acid positive in feces or anal swabs of some infected people was longer than that in the upper respiratory tract.

Therefore, increasing anal swab nucleic acid detection can improve the detection rate of infected patients and reduce missed diagnosis.

Although nucleic acid detection has the advantages of high sensitivity and short detection cycle, it also has some disadvantages.

Different from the direct isolation of live virus from human body, nucleic acid detection adopts an indirect method, which may cause false negative.

Anal swab & test tubes

Anal swab & test tubes

How to sample for anal swab test

1. Soak the swab in normal saline, insert it into the anus of 2-3 cm, wipe it from the folds around the anus, or rotate it gently in the anus. And then insert it into the test tube containing normal saline.

2. For fecal swab culture, all the above operations require the use of sterile equipment and the swab into a sterile test tube.

anal swab

Sample collection swab :How much do you know about nucleic acid testing

Sample collection swab :How much do you know about nucleic acid testing

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Sample collection swab:How much do you know about nucleic acid testing

The spread of the covid-19  is continuing worldwide. Although the global scientific and medical circles are making every effort to fight the epidemic, it is difficult to predict how the epidemic will develop in the future, and its impact on social development has appeared in many aspects.

Currently, COVID-19 cases are over 220 million.

Currently, COVID-19 cases are over 220 million.

In order to fully control covid-19, the general screening of local areas shall be carried out if necessary.

After identifying the detection object, how to detect it?

1、 What specimen to choose?

According to the recommendations of the American infectious diseases association, the following specimens were used:

The sensitivity of simple oropharyngeal swab may not be enough, so it is relatively easy to miss diagnosis.

The sensitivity of alveolar lavage fluid is the highest, but it is obviously not suitable for universal screening.

Nasopharynx swabs and other operations should also be standardized.

Improper operation may make the quality of collected samples not high, thus increasing the risk of missed diagnosis.

2、 Select the time of detection

It is very important for COVID-19 to measure RNA and choose the right time. One analysis included 7 studies, including 2 unpublished reports, to evaluate the detection efficiency of RT-PCR at different time points after exposure to virus :

On the day of exposure to the virus, the possibility of detecting viral RNA is almost zero;

On the fifth day after exposure, that is, the first day after infection, the detection probability was 62%

About the 8th day after exposure, i.e. the 4th day after infection, the probability of detection was 80%

On the 21st day after exposure, i.e. the 17th day after infection, the detection probability was 34%

In conclusion, exposure to COVID-19 may require multiple tests to avoid missed diagnosis. However, it is not recommended to repeat the test within 24 hours of the previous test; The interval between two tests shall be at least 24 hours.

After 28 days of exposure to the virus, if the infection has not been diagnosed, it is not necessary to continue the detection; At this time, it is almost certain that it is not infected.

Nucleic acid detection throat swab  sampling process

The COVID-19 nucleic acid was novel coronavirus, which was collected from sputum or lower respiratory tract by real-time fluorescent RT-PCR. If positive, the new coronavirus could be diagnosed. This is the basis for the diagnosis of new type of pneumonia. In addition to detecting the nucleic acid of the virus, it can also detect whether the virus in the specimen is highly homologous with New Coronavirus. If so, it can be diagnosed as New Coronavirus pneumonia. The virus is highly infectious and has the characteristics of human to human transmission, but the virus is sensitive to temperature and sunlight and ultraviolet rays.

Sample collection, nasopharyngeal swab sampling

Sample collection, nasopharyngeal swab sampling

Sample collection, Oropharyngeal swab sampling

Sample collection, Oropharyngeal swab sampling

The flocked swab is the best raw material for sampling swabs.

 

 

reference

1，Infectious Diseases Society of America Guidelines on the Diagnosis of COVID-19, updated December 23, 2020. https://www.idsociety.org/practice-guideline/covid-19-guideline-diagnostics/ (Accessed on January 14, 2021).

2，Kucirka LM, Lauer SA, Laeyendecker O, et al. Variation in False-Negative Rate of Reverse Transcriptase Polymerase Chain Reaction-Based SARS-CoV-2 Tests by Time Since Exposure. Ann Intern Med 2020; 173:262.

 

COVID-19 Testing: Method of collecting virus swab samples

COVID-19 Testing: Method of collecting virus swab samples

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COVID-19 Testing: virus swab

According to New Coronavirus’s pneumonia diagnosis and treatment plan (trial version fourth), to diagnose suspected cases of New Coronavirus pneumonia, we need one of the following etiological evidence:

1. Respiratory tract specimens or blood samples were detected by real-time fluorescence RT-PCR for New Coronavirus nucleic acid positive.

2. Novel coronavirus is highly homologous to respiratory tract specimens or blood samples.

Therefore, novel coronavirus pneumonia is an indispensable part of the specimen collection. So, where to collect specimens? How to collect specimens? How can medical staff protect themselves during collection? What should I do after collection?

How to obtain a virus swab specimen?

We all know that the collection method of virus swab specimen is basically to use the swab to sample the infected place or part of the virus, and then put it into the virus sampling tube containing preservation solution without touching other places, so as to conduct virus detection later.

Specimen type:

1. Upper respiratory tract specimens: including pharyngeal swab, nasal swab, nasopharyngeal extract, etc.

2. Lower respiratory tract specimens: including deep expectoration fluid, respiratory tract extract, bronchial lavage fluid, alveolar lavage fluid and lung tissue biopsy specimens.

3. Blood samples: try to collect anticoagulants in the acute stage within 7 days after onset. The collection volume is 5 ml, preferably fasting blood.

4. Serum samples: try to collect double serum in acute stage and recovery stage.

5. Conjunctival specimens: conjunctival swab specimens shall be collected for cases with ocular infection symptoms.

6. Stool samples: Patients with diarrhea symptoms need to collect stool samples.

COVID-19 Testing: Common virus swab sampling methods

Methods of throat swab collection

Inform the patient of the purpose of examination, collection method and collection time. It is best to collect specimens before using antibiotics.

Objective: To study the clinical effect of the method

Secretions were collected from pharynx or tonsil for bacterial culture or virus isolation to assist in clinical diagnosis and treatment.

Prepare sampling supplies:

Disposable sampling swab, virus sampling tube, tongue depressor and biological bag

Operation method:

(1) Ask the patient to sit down, tilt his head back, open his mouth and make a “ah ~” sound

(2) The sampling personnel shall fix the patient’s tongue with a tongue depressor, and use a disposable pharyngeal swab to pass through the root of the tongue to the posterior pharyngeal wall, tonsil recess and lateral wall;

(3) Repeatedly wipe 3-5 times to collect mucous membrane cells or sputum

(4) . gently remove the swab to avoid touching the tongue, the suspension, the oral mucosa and saliva

(5) Put the swab into the virus sampling tube gently and vertically. The sampling personnel shall disconnect the tail of the flocking swab and leave the tail of the swab completely in the tube.Be careful that the swab does not touch the wall of the tube.

(6) . tighten the cap, mark it, and seal it in the biosafety bag.

(7) The sampling personnel shall send the samples to the laboratory for testing in time. If the sampling personnel cannot send the samples for inspection in time, they shall store them at low temperature

Methods: nasal swabs were collected

Nasopharyngeal swab_COVID-19 Testing: Method of collecting virus swab samples

Nasopharyngeal swab_COVID-19 Testing: Method of collecting virus swab samples

(1) Ask the patient to sit down with his head slightly back and remove the secretion from the front nostril first;

(2)measure the distance from nostril to ear base with swab and mark with finger

(3)gently insert the swab into the nostril until it is marked or stopped when there is obstruction. Let the swab stay in the nose for 15-30 seconds, and then rotate it gently three times.

(4) slowly pull out the nasal swab and quickly put it into the virus sampling tube. Then break the swab, leave its tail and place it completely in the tube.

(5) tighten the cap, mark it, and seal it in the biosafety bag.

(6) If we need to collect from both nostrils, we should use a swab.

(7) It should be sent to the laboratory for testing in time. If it can not be sent for inspection in time, it should be stored at low temperature.

Generally speaking, COVID-19 testing: the main methods of collecting virus swabs are nasopharyngeal sampling and oropharyngeal sampling.

 

Is COVID-19 pneumonia more infectious than SARS?

The effectiveness of infectious disease transmission depends on its basic regeneration index. Novel coronavirus pneumonia is more infectious than SARS at present, with a basic regeneration index of SARS higher than that of SARS.

The novel coronavirus pneumonia is a new disease, and the population is generally immune. Everyone is susceptible and susceptible to infection. The incubation period of COVID-19 also has the ability to transmit, and it has longer infection time. The novel coronavirus pneumonia novel coronavirus pneumonia is also typical of the clinical symptoms, and asymptomatic infections are also contagious, so the infection of new crown pneumonia is higher than SARS.

About us

Cell medical (Dongguan) Co., Ltd.  is a swabs manufacturer. We produce and sell virus sampling related products. Such as virus sampling flocked swabs, VTM kits and virus transport media, and have multiple automatic production lines.  We support customized production, OEM, etc., and develop products with customers.