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What is Viral Transport Media

Posted on 2022年3月14日2022年3月30日 by Dr. Lee

What is VTM?

VTM is the acronym for Viral Transport Media, which contains nutrients that carry the virus and keep it active so that the sample can reach the laboratory for testing.

VTM virus preservation solution is suitable for the collection, preservation and transportation of common virus samples.

Such as new coronavirus, influenza virus, hand, foot and mouth virus. It can collect throat swabs, nasal swabs or tissue samples from specific parts.

And the stored samples can be used for subsequent nucleic acid extraction or Purification and other clinical experiments.

Virus Transport Medium (VTM) Definition

The virus transport medium sampling tube is a complete set of centrifuge tubes for sampling and transporting viruses.

What is Viral Transport Media

Such as new coronavirus, influenza virus, hand, foot and mouth virus in the microbial sampling transport tube.

Also known as the specimen transport tube. commoditized products.

A nutrient is used to carry and maintain the viability of test specimens (viruses) for the identification and further processing of samples in laboratories.

Viral Transmission Media Principles (VTM)

Since there is no cellular structure. Viruses cannot replicate themselves, but instead, invade genes into host cells and replicate new viruses with the help of the latter’s replication system.

After the virus sample is collected, in order to maintain the activity of the virus sample and prolong the survival time of the virus in the sample, the sampling swab will be put into the transportation medium for storage and transportation.

Transportation medium composition and inspection principle:

1. The combination of various antibiotics has broad-spectrum antibacterial and antifungal effects.

2. As a protein stabilizer, bovine serum albumin (BSA) can form a protective film on the protein shell of the virus, making it difficult to decompose and ensuring the integrity of the virus

3. The neutral environment constructed by Hank’s buffer helps to increase the survival time and infection stability of the virus.

4. Phenol red is an acid-base indicator, the discoloration area is 6.6 (yellow)-8.0 (red), and it is red at 7.2-7.4.

Vtm Kit

The most stable pH value of the viral outer layer protein is 7.2-7.4, which is also the pH value range of Hank’s buffer. If contaminating molds or bacteria are not inhibited by antibiotics, they can multiply, releasing carbon dioxide and lowering the pH.

At this time, the appearance of the sampling solution will change from red to yellow.

Notes:

To produce an isotonic solution containing proteins to protect viral structures, antibiotics to control microbial contamination, and one or more buffers to control pH.
Use the concept of assays such as nucleic acid amplification assays (NaAt) to prepare viral delivery media while preventing samples such as drying.

Composition of viral transport media (VTM Kits)

There are mainly the following parts:

1. Sampling swab with disposable sterile plastic rod/rayon head;

2. Sterile sampling tube containing 3ml of virus maintenance solution;

In addition, there are additional parts such as a tongue depressor, a biosafety bag, etc.

Viral delivery media typically have protective protein components, antimicrobial agents to prevent the potential for contamination, and buffers to maintain the pH of the solution.

Some VTMs also have additional ions and minerals that help keep such samples viable.

How to use Virus Transfer Media?

Liquid transfer media are primarily used to transfer the swab or material from the collection swab into the media.

It facilitates the collection and transport of samples in areas where it is not available.

Precautions for use:

1. Before sampling, indicate the sample information on the label of the cryopreservation tube;

2. According to different experimental purposes, use sampling swabs to sample at the corresponding parts (the most commonly used are nasopharyngeal swabs, pharyngeal swabs are the oral swabs in the product);

3. Immediately after sampling, put the swab into the sampling tube containing the virus transport medium, break it at the breaking point, and tighten the tube cover;

4. The specific sampling methods are as follows:

a) Nasal swab: Gently insert the flocked swab head into the nasal palate, stay for a while and then slowly turn to exit. Swab the other nostril with another swab, immerse the swab head in the sampling solution, break it at the breaking point, and discard it.

b) Pharyngeal swab: Wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with a flocked swab, and also immerse the swab head in the sampling solution and discard it.

Flocked Cotton Throat Oral swab 15Cm

Specimen Collection Swab

Posted on 2022年3月3日2022年3月22日 by Dr. Lee

Specimen Collection Swab

Disposable Specimen Collection Swab is suitable for the collection of microorganisms, which can assist hospitals or laboratories in virus detection or research virus sampling.

For disease detection in the human oral cavity or nasal cavity.

How to use specimen collection swab

To collect oral epidermal cells of nasal virus samples, the cells or samples are stored in a collection tube and then transferred to a laboratory for testing.

Specimen collection swab how to use?

Step 1: Prepare the required materials to ensure that you do not have a cold or runny nose so as not to affect the test results, tear open the Cell medical flocked swab sampling kits, hold the handle and gently insert the sampling swab into the nasal cavity.

Step 2: Gently rotate the swab deep into the nasopharynx for 3-5 turns, and then take it out slowly. Be careful to handle it gently, and do not touch the head of the swab with your hands or other objects.

Step 3: Put the extracted sample into the sample collection tube, break the handle, seal the cover of the sample collection tube to ensure sealing.

And then complete the sampling at random.

What is a virus sampling swab kit?

swab test swab kits

The virus sampling swab set is a virus sampling and transfer product for virus nucleic acid detection.

Including a sampling swab (Specimen Collection Swab), a sampling tube and a virus preservation solution.

It can be used for nasopharyngeal and oropharyngeal covid-19 sampling swab sets, the virus preservation solution is also called virus transport medium (Viral Transport Media), referred to as VTM.

According to the composition and function of virus preservation solution.

Including non-inactivated type and inactivated type.

Non-inactivated virus sampling swab kits

In the non-inactivated new coronavirus sampling swab kit. The components of the virus preservation solution usually include nutrients (such as salts, proteins, glucose), bacteriostatic agents, buffers, phenol red indicator, etc. required for the survival of the virus.

This virus preservation solution can maintain the activity of the virus to the greatest extent and not only for virus detection but also for virus culture and isolation.

Inactivated virus sampling swab kits

In the inactivated new coronavirus sampling swab kit, the components of the virus preservation solution usually include buffers, virus lysing agents (usually guanidines), surfactants, etc. This preservation solution can directly lyse the virus to release nucleic acid, which is convenient for subsequent nucleic acid detection.

What we need to pay attention to is that whether it is an inactivated type or a non-inactivated type, the virus sampling tube is sterile.

There are no other microorganisms that could cause false detections due to viral breakdown or other influences after sampling.

After flocked swab sampling, if a collection tool or preservation solution of poor quality is used, it will affect the subsequent test results and even cause false positives for misdiagnosis.

Different types of preservation solutions have their advantages and disadvantages according to different situations.

Recommended using a virus preservation solution of good quality.

How to Use Specimen Collection Kit

Posted on 2022年2月22日2022年3月22日 by Dr. Lee

What is a Specimen Collection Kit?

Sample collection kits provide all the components needed to secure samples and send them to laboratories for diagnostic. Environmental sampling and research purposes. Timely collection and processing of samples are critical to ensure accurate results and prevent analytical errors.

Specimen Collection Kit Contains:

Individually packaged sterile specimen collection flocked swab;
10ml virus sampling tube, containing 3ml Viral Transport Media;
Sterilization packaging, Individual in Paper peeled pouch;

The novel coronavirus (2019-nCoV) is an RNA virus. Improper storage or transportation of samples after collection may result in the degradation of part of the nucleic acid and affect the test results;

In addition, samples that have not been inactivated virus can easily lead to indirect infection of operators.

The covid-19 sampling kit independently developed by Cell medical can not only prevent the degradation of viral RNA but also inactivate the virus.

While ensuring the test results, it can reduce the risk of biological infection during the inspection process.

Product description

Viral Transport Medium Chin, VTM kits For COVID-19. Cell medical not only produces sampling swabs but also virus sampling tubes.

Analyzing material effectively starts with collecting samples with the right tools, whether it’s collecting samples from the body to aid in medical diagnosis, identifying potentially harmful pathogens from the environment.

Or collecting samples from crime scenes to aid in investigations.

However, it is also important to ensure that contaminants do not come into contact with the sample as it travels to the laboratory. Given the plethora of options available for each of these missions, choosing the right collection and transport vehicle can be overwhelming. This is where a specimen collection kit can prove invaluable.

Features:

1. Safety: Sampling and virus inactivation are carried out simultaneously, which greatly reduces the operator’s infection risk;

2. Stability: keep the sample stable, avoid nucleic acid degradation, and ensure the accuracy of the test results;

3. High efficiency: Inactivated virus samples can be detected in conventional PCR laboratories, and the collection tube can be adapted to the Tianlong automated nucleic acid extraction system, which improves the detection efficiency of samples.

Instructions:

What is the Specimen Collection Kit?

As pointed out over, example collection packages have shown very usefully in securing examples for analysis in a large range of clinical, environmental and research study applications.

Nasopharyngeal samples collected by swabbing the upper retronasal vocal cords can supply analysis confirmation for a series of viral as well as bacterial infections, consisting of influenza, COVID-19, enterovirus D68, as well as other respiratory system conditions.
Fecal samples can be gathered by anal or fecal swabs as well as can aid clinicians detect a series of intestinal microbial infections.
A vaginal swab example can help determine the visibility of Group B Streptococcus or Streptococcus agalactiae, a type of microorganisms found in the digestive system and also birth canals of a quarter of pregnant ladies, to name a few conditions.
Food sampling is not just important for recognizing the potential presence of foodborne pathogens but is a crucial step in maintaining conformity with FDA food security requirements.

How to choose the right specimen collection kit?

It is necessary to discover a sampling collection set created for your particular end-use. This consists of using the flocked swab that best matches your sampling collection needs. As an example, foam swabs might use elution characteristics best matched for environmental and also food sampling, while polyester or crowded swabs may supply a combination of collection attributes and also person comfort far better fit for medical usage.

Nonetheless, it is not constantly necessary to utilize a sample collection package created for usage with the example cpu’s very own brand name of the fluid transfer system. Validation examinations can aid clinicians, food mill and also other samplers, and also labs recognize precise and also affordable sample collection kits. Verifying shipping systems from several suppliers has come to be a common practice in research laboratories around the globe, not just to ensure sustainable supply, but likewise to compare item top quality and expense.

About COVID-19 Swab Test

Posted on 2021年12月1日2022年3月22日 by Dr. Lee

About COVID-19 Swab Test

The new crown virus has mutated continuously.

From Delta to Omi Keron, the COVID-19 virus has continued to mutate, and the global epidemic is prevalent at a high level.

Canada announced that all entry personnel from all countries except the United States are required to be tested for the new coronavirus.

At this time, whether you are leaving the country or entering the country.

I even worry or doubt whether I have contracted the coronary disease.

Perhaps understanding the new coronavirus test has become a compulsory course for most people.

What is the new crown swab test, how to collect a nasopharyngeal swab? Is nasal swab sampling painful? If your test result is positive, what do we need to do? Why do most virus tests choose to flock swabs? Is nasopharyngeal swab accurate? Should you be tested for nasopharyngeal swab samples?

We talked with Dr. Li of Cell medical to learn more.

What is the COVID-19 test and what are the test methods?

Nucleic acid detection (RT-PCR Test) method, antibody detection, and antigen detection.

A swab test simple, effective, and fast way to detect COVID-19.

Including nasopharyngeal swab testing and oropharyngeal swab testing.

Medical staff will use flocked swabs to take samples from the nose and throat and put them into the virus sampling tube.

There are two types of swab sample testing: molecular test (PCR) and antigen test.

How to perform a COVID-19 nasal swab test?

The COVID-19 nasal swab test involves inserting a 6-inch flock (a long stick with a very soft brush) into the cavity between your nose and mouth (nasopharynx).

The medical staff inserts the flocking swab into each nostril for about 15 seconds and rotates it several times to collect enough samples for testing.

The samples are then sent to the laboratory for testing, and our test results are usually known within or within the next day

Since the nasopharynx is in a sensitive position of the body, it will produce a lot of very strange sensations.

On the one hand, the swab is inserted deeply into your nose and it irritates the lacrimal glands.

At this point, you don’t know if you are moved to tears or you are crying in pain.

The nasal swab test is not very painful, but it is very uncomfortable. Some people may have nausea during the nasopharyngeal swab test. Nasal swab painful.

Because the swab will also touch the back of your throat, this reaction is normal.

Whether it is tearing or nausea, nasal swab testing is a common phenomenon.

Don’t worry, everything is normal.

Are there other types of COVID-19 swab test methods available?

Yes, an oropharyngeal swab is one of the options.

Of course, if you want to experience another feeling, an anal swab test is also a good choice.

But they are not as sensitive as the COVID-19 nasal swab test.

Israel’s development of the new coronavirus “saliva detection method” saliva detection still needs scientists to confirm on a larger scale. There is no conclusion yet, but it is promising.

In addition to nucleic acid testing for the detection of viral genetic material, there is also antigen testing.

Currently, nasal swab testing for COVID-19, including nucleic acid testing, is still the best choice.

How about the covid test kit at home? Are their results reliable?

The home covid test kit sounds very attractive.

Home covid test usually refers to individuals collecting samples of themselves or their family members through sampling swabs.

We put the collected samples in test tubes with a virus transport medium, and then transport them to the testing facility.

Although you can use the Home covid test kit to test COVID-19 safely and comfortably in your own home.

However, Dr. Li suggested that it should be carried out at a professional testing institution.

Because the quality of the samples we collect and the integrity of the samples during transportation will affect the sampling results.

How accurate is the nasal swab test for COVID-19?

How to obtain a precise nasopharyngeal swab specimen?

Polymerase chain reaction (PCR) uses a piece of DNA as a template.

Then, with the participation of DNA polymerase and nucleotide substrate, the DNA is amplified to a sufficient amount for structural and functional analysis.

Pcr nasal swab test can be said to be very sensitive.

Scientists use PCR technology to amplify a small amount of RNA in the sample into deoxyribonucleic acid (DNA) and replicate it until COVID-19 (if present) is detected.

The following may affect the accuracy of PCR nasal swab sampling.

1. Smoking, drinking or chewing gum 30 minutes before use may also interfere with the test results.

2. Touching the swab with the hand or other objects and using the tip of the cotton swab will affect the accuracy of the detection.

Of course, the professionalism of the most important sampling personnel, we have to choose a professional testing agency for testing. And professional VTM Kits.

What should I do if the new coronary pneumonia nasal swab test is positive?

Very bad news!

If the COVID-19 nucleic acid test is positive, a single room isolation treatment should be carried out immediately.

Consultation by in-hospital experts or attending physicians will conduct multiple tests.

Under the premise of ensuring safety, the patient is safely transferred to a designated hospital for diagnosis and treatment.

Don’t worry. You need to actively cooperate with treatment

Otherwise, life is likely to be in danger, and large-scale infections will also occur.

In addition, proper physical exercise should be carried out in life to enhance the individual’s immunity. Colds and fatigue should be avoided.

Don’t do strenuous exercise, so as not to aggravate the disease.

A friend around me was infected with COVID-19. But my nasopharyngeal swab was negative. Is there any way to confirm that I have had it or am I immune to it now?

Certainly.

Antibody test, also called serological test, can detect the antiviral protein IgM and IgG antibodies produced by your immune system in the blood.

What are the types of samples for serum antibody testing?

It is generally blood, including serum, plasma, and whole blood.

PCR swab test, antibody test, and antigen test difference (Please click here)

China universal viral transport medium factory manufacturers

Posted on 2021年9月16日2022年3月30日 by Dr. Lee

China universal viral transport medium factory and manufacturers

Product specification

VTM KIT_Virus sampling tube

[common name]: disposable virus sampling tube, viral transport medium kits,universal viral transport media.

[specification and model]: 3ml / piece, 100 pieces / bag

[main structure and performance]

It usually consists of swabs and/or preservation tubes containing viral transport medium.

[scope of application]

For sample collection, transportation and storage.

[action principle]

After sterilizing the sampling tube, add virus stable components such as Hank’s base solution, which can maintain the activity of virus in a wide temperature range, reduce the decomposition speed of virus and improve the positive rate of virus isolation.

Description

Viral Transport Medium (VTM) allows the safe transfer of viruses, chlamydia and mycoplasma for further research, including conventional cell culture methods, diagnostic tests, and molecular biology techniques.

Commercially prepared viral transport media are available in a screw cap plastic tube containing buffered proteins (serum, albumin or gelatin) and antibiotics.

Viral transport medium composition

Our viral transport media contains Hanks Balanced Salt Solution (HBSS) with Calcium and Magnesium and contains heat-inactivated Fetal Bovine Serum, Gentamycin and Amphotericin B.

The composition and the manufacturing of the Viral Transport Medium follow the WHO and the CDC recommendations.

[contraindications, precautions, warnings and suggestive instructions]

1. After sampling with a virus sampling tube, the sampler shall submit it for inspection as soon as possible.

At the same time, the transportation personnel shall immediately transport the virus samples at a low temperature of 2-8 ℃.

The results showed that the virus samples could be stored at 2-8 ℃ for 48 hours; But for long-term storage, below – 20 ℃ and – 70 ℃.

If the sampler does not immediately transport at low temperature or store at the specified temperature after sampling. It will affect the positive rate of virus sample separation in the later stage.

2. prohibited to use it for the sampling of bacterial samples. The antibiotics contained in the sampling solution can inhibit bacteria.

3. forbidden to directly contact the collected patient, and forbidden to sample the patient after wetting the swab.

4. The sampling personnel shall collect virus samples in strict accordance with the sampling procedures to ensure the accuracy of sampling location. At the same time, the sampling intensity is uniform and appropriate, otherwise, it will affect the positive rate of virus isolation.

5. It is forbidden to use the expired or damaged product package.

6. The experimenter shall test the samples in a laboratory meeting the safety level.

Factory profile

Virus transport medium factories, Cell medical is a professional manufacturer of sampling swabs and disposable virus sampling tubes (viral transport medium).

The factory is located in Dongguan, China, with a daily production capacity of more than 1 million sets, aseptic filling technology and aseptic swab production technology.

China universal viral transport medium factory and manufacturers_Cell medical (Dongguan) Co., Ltd.

China VTM tube factory GMP factory_Cell medical (Dongguan) Co., Ltd.

Cell medical (Dongguan) Co., Ltd., vtm kit factories, vtm swab factory, produce flocked swabs, VTM kits, virus transport medium, universal viral transport media, with multiple automatic production lines.

We support custom production, OEM and so on, and develop products with customers.

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