Tag Archives: virus sampling

Respiratory Specimen Collections

Respiratory Specimen Collections

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Respiratory Specimen Collections

Today, with the increasingly severe international COVID-19 epidemic, the correct collection of respiratory samples has become an important prerequisite for ensuring the accuracy of nucleic acid detection results.

However, how to correctly collect respiratory samples? Cell medical (Dongguan) Co., Ltd. summarizes as follows:

Why should respiratory tract samples be collected standardized and correctly?

1. Clear diagnosis;

2. Observe the condition and treatment effect;

3. Guide the formulation of treatment measures;

4. Sample quality directly determines the reliability of test results.

 What are the types of respiratory samples?

1. Upper respiratory tract samples (including swabs, nasal specimens, nasopharynx extracts, pharyngeal gargle and nasal lotion, etc.);

2. Lower respiratory tract samples (such as sputum, tracheal aspirate, lung lotion, etc.);

When is the best time to collect respiratory samples?

Respiratory tract samples were collected as soon as possible after symptoms appeared;

Collect as much as possible within 72 hours of the patient’s fever;

Try to collect before the use of antiviral drugs;

If possible, collect multiple times in multiple days;

What sampling tools are needed to collect respiratory tract samples?

1. Virus sampling kits, including flocked swab, virus sampling tube, virus transport medium, etc;

2. Refrigerated transport box;

3. Personal protective equipment: protective mask (N95), protective glasses.

 How to collect respiratory tract samples?

Oropharyngeal swab sampling process:

Oropharyngeal swab sampling pictures

Oropharyngeal swab sampling pictures

1. The sampler first let the patient sit down, then let the patient tilt his head back and open his mouth.

2. Press the front 2 / 3 of the patient’s tongue with a tongue depressor, and wipe the posterior pharyngeal wall and bilateral tonsils with a nylon flocked swab for 3-5 times to avoid touching the tongue.

3. Take out the rear sampling tube, break the plastic handle at the contact part of the hand, soak the swab into the sampling solution, and tighten the tube cover.

Nasopharyngeal swab:

"nasal

Note : do not use too much force, but try to be as deep as possible, usually more than 5cm, stay for several seconds to absorb secretions, gently rotate and take out the swab, place the sampling tube, break the plastic ellipse at the contact part of the hand, and soak the test piece into the sampling solution, Tighten the pipe cover.

Respiratory Specimen Collections preservation requirements

Those that can be detected within 24 hours can be stored at 4 ℃ and frozen at – 70 ℃ for more than 24 hours.

 

Swab test

Swab test | Disposable virus sampling swab

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Swab test | Disposable virus sampling swab

Disposable virus sampling swab, I believe you are no stranger in the past two years. Because most of the detection tools used for nucleic acid test are disposable sampling swabs. Usually, we see most nasal swabs and pharyngeal swabs. But disposable virus swabs are not only useful for nucleic acid testing. What else can a disposable sampling swab do? Let’s get to know.

What can a disposable virus swab be used for?

In addition to nucleic acid detection, scientists can also use disposable sampling swabs for microbial collection. They are usually used for virus detection and research in hospitals or laboratories. Researchers use disposable sampling swabs to detect biological samples in natural cavities of human or animals. Such as throat, nose, mouth, vagina and so on. It is necessary to collect oral epidermal cells and nasal virus samples with a sampling swab, store the cells and samples in the sampling tube and transfer them to the laboratory for examination.

Oropharyngeal swab sampling

Oropharyngeal swab sampling

Operation (use) method of disposable sampling swab:

Take pharyngeal swab as an example:

① Tear open the packaging bag of the disposable sampling swab and take out the disposable sampling swab from the bag;

Note: please do not let the disposable sampling swab touch the place outside the throat to avoid pollution.

② Put a disposable sampling swab into the mouth and quickly wipe the palatal arch and throat (or lesion) on both sides

③ Take out the swab, put it into the disposable virus sampling tube, break the disposable sampling swab at the broken place and discard the tail;

④ Tighten the sampling tube, put the virus sampling tube into the biosafety bag and submit it for inspection in time.

Note: please do not shake the virus sampling tube excessively to avoid dilution and loss of samples. After sampling, do not touch other objects with a disposable sampling swab.

How to carry out COVID-19  nasal swab test?

The doctor shall wear protective clothing, mask, gloves and mask to let the patient take a proper position.

Gently insert the flocked sampling swab into the nose, rotate it for three times, take it out and put it into the sample tube with protective solution, and screw the cover. Then label it, write the date, and send it to the laboratory for nucleic acid test of nasopharyngeal swab.

 

What is a COVID-19 swab test?

Swab test, namely nucleic acid test. The substance test by nucleic acid is the nucleic acid of virus. Nucleic acid detection is to collect the presence of viruses in the respiratory tract, blood or feces of patients, and determine whether they are infected by COVID-19. Therefore, once detected as nucleic acid “positive”, it can prove that there is a virus in the patient’s body.

SARS-CoV-2 nucleic acid detection process.

The usual sample types include throat swabs, nasal swabs, sputum, bronchoalveolar lavage fluid, alveolar lavage fluid and so on.

The sampling personnel shall conduct PCR detection as soon as possible after obtaining patient samples.

After the testing institution receives the sample, the laboratory personnel shall extract nucleic acid from the sample. And the nucleic acid extraction reagent shall use the nucleic acid extraction kit specified in the approved product manual.

Disposable virus sampling swab material.

At present, because of its stable and reliable performance and excellent absorption and elution capacity, nylon flocked swabs are widely used in COVID-19 sampling.

Swab test result

Swab testing for novel coronavirus pneumonia usually takes 4-6 hours to produce results, while rapid examination takes 2-3 hours. However, the reagents used in each hospital are different, so the time of results is also different.

COVID-19 positive results：Maybe infected.After 24 hours of self isolation, the positive patients need to conduct swab test again.

If the two results are positive, the next step is to improve CRP, blood routine, chest CT and other tests to determine whether COVID-19.

When COVID-19 nucleic acid test is negative once, it should be combined with the clinical symptoms and epidemiological contact history of patients. Because a COVID-19 nucleic acid swab test is negative, it can not completely exclude the infection of COVID-19.

 

Comparison of advantages of various testing methods of COVID-19

Comparison of advantages of various testing methods of COVID-19

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advantages of various testing methods

The detection methods of COVID-19 mainly include nucleic acid detection, antibody detection and antigen detection. Due to the low detection rate of antigen, the SARS-CoV-2 detection mainly focuses on antibody and nucleic acid detection. Nucleic acid detection has the characteristics of early diagnosis, high sensitivity and specificity; However, antibody detection is convenient and rapid, which can be used as a supplementary means of nucleic acid diagnosis. Due to the limitations of “false positive” and “false negative” antibody detection, it is not suitable for general population screening such as returning to work,  and school, nor for epidemiological investigation in low epidemic areas.

NAT Detection process

The detection procedure needs to go through five steps: sampling, sample retention, preservation, nucleic acid extraction and computer detection.

First, collect samples according to the instructions of the kit. The sample types include pharyngeal swab, nasal swab, sputum, bronchial lavage fluid, alveolar lavage fluid, etc.

Because RNA is easy to degrade, flocked swabs without RNase and storage tubes without RNase are used when collecting samples.

After obtaining the patient’s sample, it needs to be tested as soon as possible. If it cannot be tested immediately, it needs to be packaged at low temperature and sent to a special testing institution for testing.

After receiving the sample, the testing institution shall extract the nucleic acid from the sample. The nucleic acid extraction reagent shall use the nucleic acid extraction kit specified in the approved product manual.

Finally, fluorescence PCR nucleic acid detection, that is, machine detection. It takes 70-80 minutes to amplify the extract by fluorescence PCR.

Sample collection, oropharyngeal swab or nasopharyngeal swab

Sample collection, oropharyngeal swab or nasopharyngeal swab

NAT Detection process Nucleic Acid Testing

NAT Detection process Nucleic Acid Testing

Antibody detection

After the virus invades the human body, the human body will produce corresponding specific antibodies for defense.

According to different molecular structures and antigen specificity, scientists divide immunoglobulins into five categories: IgG, IgA, IgM, IgD and IgE.

After the antigen enters the human body for the first time, plasma cells secrete antibodies after a certain incubation period. IgM first appeared, but the antibody had short maintenance time, low concentration and low affinity. It lasted for several days to several weeks in the blood. It was a diagnostic index of acute infection; When IgM is about to disappear, the content of IgG reaches the peak. IgG appeared late, but its concentration was high, its maintenance time was long and its affinity was high. The positive serum IgG indicates the middle and late stage of infection or previous infection.  But the antibody content increases rapidly in the short term, which may be several times to dozens of times higher than the original antibody content. It is mainly IgG, which lasts a long time in the body, and IgM rarely increases.

In this epidemic, the researchers studied patients with covid-19 and found that after the virus invades the human body, IgM antibody takes about 5-7 days to produce and IgG antibody takes 10-15 days to produce.

Antibody detection kits-advantages of various testing methods

Antibody detection kits-advantages of various testing methods

Comparison of advantages and disadvantages of two different detection methods

Compared with the serological detection of antibodies, Nucleic Acid Testing can detect patients in the window stage.

Early detection of infected persons is the “gold standard” for COVID-19 detection, but it requires higher detection equipment or platform.

The high sensitivity RT-PCR instrument is expensive, and the laboratory cleanliness and operator requirements are higher. In addition, nucleic acid detection takes a long time. Considering the sample transportation and sample backlog, the results can be reported as soon as 24 hours.

Compared with nucleic acid detection, blood samples detected by antibody serology are easier to obtain. If the RNA of the virus detected by nucleic acid is the direct evidence of the existence of the virus, the antibody detection is the antibody stimulated in the patient’s blood, which is indirect evidence and has a suggestive effect on the clinic.

When nucleic acid detection is negative, adding IgM and IgG antibody detection can make up for the shortcoming that nucleic acid detection is easy to cause missed diagnosis.

About us

Cell medical (Dongguan) Co., Ltd., a professional VTM kits and swabs manufacturer, has many years of experience in medical application molding and flocked treatment heads and swabs.

Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.Certificate:FDA,CE,EU,Free Sale,Export license based on (GMP).

We produce flocked swabs, VTM kits, Virus transport medium, with multiple automatic production lines.We support custom production, OEM and so on, and develop products with customers.

  • E-mail：flockedswabs@gmail.com

     

    Is anal swab nucleic acid test more accurate than nasopharyngeal swab?

    Is anal swab nucleic acid test more accurate than nasopharyngeal swab?

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    Is anal swab nucleic acid test more accurate than nasopharyngeal swab?

    Nucleic acid detection:generally carried out by sampling pharyngeal swabs in the nasal cavity and oral cavity.

    Now began to increase the anal swab test, we are also curious about this test method. So, is anal swab nucleic acid detection more accurate than nasopharyngeal swab? How to sample for anal swab test?

    Is the nucleic acid detection of anal swab more accurate than that of nasopharyngeal swab?

    In the screening of New Coronavirus pneumonia, we mainly used the oropharyngeal swab nucleic acid test. Because the reason is convenient and quick, suitable for large-scale screening. The accuracy of nasopharynx swab for new crown nucleic acid detection is higher than that of oropharyngeal swab, but the human body feels uncomfortable.

    In last year’s test, researchers found that many infected people had negative pharyngeal nucleic acid.But their anal swabs were still positive for longer.

    The combination of the two in nucleic acid detection can improve the detection rate and reduce the proportion of missed diagnosis.

    However, anal swab sampling is inconvenient and not suitable for large-scale screening and testing in the community.

    For some asymptomatic or mild infections, the condition recovers quickly after infection. And the pharyngeal nucleic acid may not be detected in 3 to 5 days.

    The researchers found that the duration of nucleic acid positive in feces or anal swabs of some infected people was longer than that in the upper respiratory tract.

    Therefore, increasing anal swab nucleic acid detection can improve the detection rate of infected patients and reduce missed diagnosis.

    Although nucleic acid detection has the advantages of high sensitivity and short detection cycle, it also has some disadvantages.

    Different from the direct isolation of live virus from human body, nucleic acid detection adopts an indirect method, which may cause false negative.

    Anal swab & test tubes

    Anal swab & test tubes

    How to sample for anal swab test

    1. Soak the swab in normal saline, insert it into the anus of 2-3 cm, wipe it from the folds around the anus, or rotate it gently in the anus. And then insert it into the test tube containing normal saline.

    2. For fecal swab culture, all the above operations require the use of sterile equipment and the swab into a sterile test tube.

    anal swab

    Nasal mini-tip flocked swab

    Nasal mini-tip flocked swab

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    Nasal mini-tip flocked swab Medical Nasopharyngeal swab

    Nasopharyngeal swabs are a means of detection. Doctors use nasopharyngeal swabs mainly for special bacterial group infection or virus infection in the nasopharynx. They will use sterile pharyngeal swabs to enter the nasopharynx and throat through the nasal cavity and wipe the mucosa. Conduct bacterial culture, virus culture and drug sensitivity test to determine our treatment plan and diagnosis. This examination is a non-invasive examination.

    Product description

    medical nasal nasopharyngeal mini tip flocked swab

    1. EO sterile, individual packing
    2. With molded breakpoint
    3. Ergonomic and anatomic design, easy to use
    4. Perpendicular nylon fibers, increased assay sensitivity

    CM-96000 NASAL SWAB

    CM-96000 NASAL SWAB

    Product Flocking swab for nasal
    Item No CM-96000
    Material Tip: 100% nylon, handle: plastic (ABS)
    Usage Single use for Nasopharyngeal excretion collection
    Size Diameter: 3mm

    Length: 150mm
    Breaking point: 80mm
    Packing 1pc/pouch, 10000pcs/ctn
    sterile, individual packing cm-96000 Packaging picture

    sterile, individual packing cm-96000 Packaging picture

    Nasal mini-tip flocked swab Packaging & Delivery

    Packaging Details
    Individual Sterile Package
    100 pcs/zip pouch
    5000pcs /carton box
    Carton Size: 52*40*30 cm
    G./W.: 6.5kg
    Port
    shenzhen

    Contact us

    • Cell medical (Dongguan) Co., Ltd.
    • E-mail：flockedswabs@gmail.com
    • No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China
    • Web：www.swabmanufacturer.com/
    Iclean nylon flocked swab

    Iclean nylon flocked swab

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    Iclean nylon flocked swab

    Product purpose:

    Iclean nylon flocked swab is ideal for bacteriological sample processing, virological cell culture, DFA test, rapid and direct test, enzyme immunoassay test, polymerase chain reaction and molecular diagnosis based test, as well as forensic identification.

    For throat sampling of respiratory viruses such as influenza, swine influenza, avian influenza, hand, foot and mouth.

    Iclean nylon flocked swab,View more

    Iclean nylon flocked swab,View more

    Characteristics of sampling swab products:

    1. It has extraordinary water absorption capacity, which can increase the number of samples collected on its surface from 20% of traditional sampling swabs to 60%.

    2. The release rate of the collected samples is more than 90%, so as to ensure the high reliability of the results.

    3. Different preservation solutions were selected for different specimen types.

    4. The plastic rod has a unique breakable design to facilitate specimen transportation.

    5. Ethylene oxide sterilization, irradiation sterilization, independent packaging.

    Iclean gene sampling series flocking DNA oral test pieces are specially used to improve the amount and quality of genomic DNA in oral test piece cells. Oral cell sample is a good non-invasive collection and detection method instead of blood. Combined with icleanhcy’s series of DNA separation and stabilization kits, it can quickly collect high-purity and complete DNA samples.

    DNA flocking swab is suitable for human and animal use. It has advantages over other DNA samples in design. Its unique sample matrix and rapid release interface are more conducive to efficient cell collection and maximize DNA yield. The test piece has been sterilized with ethylene oxide and passed the detection of human DNA contamination.

    characteristic

    The unique test substrate greatly improves the yield of DNA;

    Adult oral SWAB piece 1 to 10 μ g DNA;

    It is a good alternative to blood collection;

    Both human and animal can use it;

    Simple and fast operation;

    There are two types of single and double disinfection packaging to choose from;

    application;

    Genotype study;

    Veterinary genotyping and diagnosis;

    Parent child and genetic services;

    Forensic and DNA population studies;

    About us

    Cell medical (Dongguan) Co., Ltd. produce flocked swabs, VTM kits, virus transport medium, with multiple automatic production lines.We support custom production, OEM and so on, and develop products with customers.

    Email:flockedswabs@gmail.com

    Disposable virus sampler

    Disposable virus sampler

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    Disposable virus sampler

    ♦ Product Name:

    ① General name: disposable sampler

    ② Name: sample collection kit

    ③ Specification and model: cm-93050t, cm-93050rt, cm-93050rtz, cm-96000t, cm-96000ts, cm98000t, cm-98000tl, cm-761tz

    ♦ Package specification: 50 pcs / bag

    ♦ Intended use: disposable virus sampling tube_ Sampling swab_ Flocking swab_ Saliva collector_ Nucleic acid detection sampling kit_ Throat swab – cell medical (Dongguan) Co., Ltd.

    Medical staff use DNA samplers and disposable samplers for sample collection, transportation and storage.

    ♦ Structure composition: swabs and test tubes. Non sterile.

    Disposable virus sampler_cm-93050t

    Disposable virus sampler_cm-93050t

    ♦ Storage conditions and validity period:

    ① Products shall be stored in a clean, hygienic, dry and ventilated environment;

    ② . the temperature is 5 ℃ ~ 35 ℃;

    ③ . relative humidity ＜ 85% RH;

    ④ Product validity: two years

    ♦ usage method:

    ① Before sampling, relevant information shall be marked on the label of the sampling tube.

    ② . use the corresponding sampling flocked swab for sampling.

    ③ After sampling, quickly put the cotton swab into the sampling tube and tighten the tube cover.

    ④ . see the following for specific sampling methods:

    a) Nasal swab: gently insert the sampling head into the nasopalatine part of the nasal canal, stay for a moment, then slowly rotate and exit, put the collected specimen into the sampling tube, and tighten the cover of the sampling tube.

    b) Pharyngeal swab: wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with the sampling head, put the collected samples into the sampling tube, and tighten the cover of the sampling tube.

    c) Sampling of mycoplasma, chlamydia and Ureaplasma samples:

    Male: insert the sampling head into the urethra and rotate about 2cm. Stay for a moment and then exit. Put the collected specimen into the sampling tube and tighten the cover of the sampling tube.

    Female: wipe the mucus from the cervical mouth, insert the sampling head into the cervical tube for 1-2cm for sampling, put the collected sample into the sampling tube, and tighten the cover of the sampling tube.

    ♦ matters needing attention:

    ① . After collecting the sample, the medical staff shall completely insert the disposable sampling cotton swab into the test tube to retain the sample to the greatest extent.

    ② . the collected samples must be fresh and submitted for inspection in time.

    ③ .forbidden to use products with damaged packaging and expiration date to prevent pollution.

    ④ . forbidden to directly contact the collected patient, and forbidden to sample the patient after wetting the swab.

    ⑤ . When collecting samples, medical staff should strictly implement the sampling procedures to ensure that the sampling position is accurate and the sampling intensity is uniform and appropriate, otherwise the sample collection quality will be affected.

    ⑥ The product shall not be used after the expiration date or the product package is damaged.

    Note: this disposable sampler : for in vitro diagnosis and not for human or animal internal and external use.

    If swallowed, it may lead to serious events and cause certain irritation to eyes and skin. If it accidentally splashes into eyes, rinse with clean water.

    ♦ essential information

    Name of manufacturer: cell medical (Dongguan) Co., Ltd

    Domicile: No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China

    E-mail： flockedswabs@gmail.com

    COVID-19 Testing: Method of collecting virus swab samples

    COVID-19 Testing: Method of collecting virus swab samples

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    COVID-19 Testing: virus swab

    According to New Coronavirus’s pneumonia diagnosis and treatment plan (trial version fourth), to diagnose suspected cases of New Coronavirus pneumonia, we need one of the following etiological evidence:

    1. Respiratory tract specimens or blood samples were detected by real-time fluorescence RT-PCR for New Coronavirus nucleic acid positive.

    2. Novel coronavirus is highly homologous to respiratory tract specimens or blood samples.

    Therefore, novel coronavirus pneumonia is an indispensable part of the specimen collection. So, where to collect specimens? How to collect specimens? How can medical staff protect themselves during collection? What should I do after collection?

    How to obtain a virus swab specimen?

    We all know that the collection method of virus swab specimen is basically to use the swab to sample the infected place or part of the virus, and then put it into the virus sampling tube containing preservation solution without touching other places, so as to conduct virus detection later.

    Specimen type:

    1. Upper respiratory tract specimens: including pharyngeal swab, nasal swab, nasopharyngeal extract, etc.

    2. Lower respiratory tract specimens: including deep expectoration fluid, respiratory tract extract, bronchial lavage fluid, alveolar lavage fluid and lung tissue biopsy specimens.

    3. Blood samples: try to collect anticoagulants in the acute stage within 7 days after onset. The collection volume is 5 ml, preferably fasting blood.

    4. Serum samples: try to collect double serum in acute stage and recovery stage.

    5. Conjunctival specimens: conjunctival swab specimens shall be collected for cases with ocular infection symptoms.

    6. Stool samples: Patients with diarrhea symptoms need to collect stool samples.

    COVID-19 Testing: Common virus swab sampling methods

    Methods of throat swab collection

    Inform the patient of the purpose of examination, collection method and collection time. It is best to collect specimens before using antibiotics.

    Objective: To study the clinical effect of the method

    Secretions were collected from pharynx or tonsil for bacterial culture or virus isolation to assist in clinical diagnosis and treatment.

    Prepare sampling supplies:

    Disposable sampling swab, virus sampling tube, tongue depressor and biological bag

    Operation method:

    (1) Ask the patient to sit down, tilt his head back, open his mouth and make a “ah ~” sound

    (2) The sampling personnel shall fix the patient’s tongue with a tongue depressor, and use a disposable pharyngeal swab to pass through the root of the tongue to the posterior pharyngeal wall, tonsil recess and lateral wall;

    (3) Repeatedly wipe 3-5 times to collect mucous membrane cells or sputum

    (4) . gently remove the swab to avoid touching the tongue, the suspension, the oral mucosa and saliva

    (5) Put the swab into the virus sampling tube gently and vertically. The sampling personnel shall disconnect the tail of the flocking swab and leave the tail of the swab completely in the tube.Be careful that the swab does not touch the wall of the tube.

    (6) . tighten the cap, mark it, and seal it in the biosafety bag.

    (7) The sampling personnel shall send the samples to the laboratory for testing in time. If the sampling personnel cannot send the samples for inspection in time, they shall store them at low temperature

    Methods: nasal swabs were collected

    Nasopharyngeal swab_COVID-19 Testing: Method of collecting virus swab samples

    Nasopharyngeal swab_COVID-19 Testing: Method of collecting virus swab samples

    (1) Ask the patient to sit down with his head slightly back and remove the secretion from the front nostril first;

    (2)measure the distance from nostril to ear base with swab and mark with finger

    (3)gently insert the swab into the nostril until it is marked or stopped when there is obstruction. Let the swab stay in the nose for 15-30 seconds, and then rotate it gently three times.

    (4) slowly pull out the nasal swab and quickly put it into the virus sampling tube. Then break the swab, leave its tail and place it completely in the tube.

    (5) tighten the cap, mark it, and seal it in the biosafety bag.

    (6) If we need to collect from both nostrils, we should use a swab.

    (7) It should be sent to the laboratory for testing in time. If it can not be sent for inspection in time, it should be stored at low temperature.

    Generally speaking, COVID-19 testing: the main methods of collecting virus swabs are nasopharyngeal sampling and oropharyngeal sampling.

     

    Is COVID-19 pneumonia more infectious than SARS?

    The effectiveness of infectious disease transmission depends on its basic regeneration index. Novel coronavirus pneumonia is more infectious than SARS at present, with a basic regeneration index of SARS higher than that of SARS.

    The novel coronavirus pneumonia is a new disease, and the population is generally immune. Everyone is susceptible and susceptible to infection. The incubation period of COVID-19 also has the ability to transmit, and it has longer infection time. The novel coronavirus pneumonia novel coronavirus pneumonia is also typical of the clinical symptoms, and asymptomatic infections are also contagious, so the infection of new crown pneumonia is higher than SARS.

    About us

    Cell medical (Dongguan) Co., Ltd.  is a swabs manufacturer. We produce and sell virus sampling related products. Such as virus sampling flocked swabs, VTM kits and virus transport media, and have multiple automatic production lines.  We support customized production, OEM, etc., and develop products with customers.

     

    Nasal swab pcr test

    Nasal swab pcr test

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    Nasal swab pcr test COVID-19 Antigen Rapid Test Kit

    Detection of COVID-19 by nasal swab PCR is an accurate and reliable method. Positive detection means you may have SARS-CoV-2. Negative detection means you may not have SARS-CoV-2 when testing.

    In order to ensure your safety, if you have symptoms of COVID-19. Or people who have been exposed to the virus and tested positive should be tested.

    What is the symptom of COVID-19?

    If you have the following symptoms,if you have the following symptoms, please buy covid-19 antigen rapid test kit immediately or go to a nearby hospital for PCR test.

    1.Fever;

    2.General fatigue;

    3.cough;

    4.Dyspnea or even respiratory failure；

    5.Nausea;

    6.vomiting;

    7.abdominal pain;

    8.diarrhea;

    Symptoms of New Coronavirus pneumonia are not necessarily typical, and some patients may not have clinical manifestations at the early stage of New Coronavirus infection. Then clinical manifestations will appear, that is, “asymptomatic infection” in incubation period.

    In other words, try to avoid activities in places with crowd gathering, closed space and poor ventilation.

    What is nasal swab PCR test？

    Polymerase chain reaction (PCR) uses a piece of DNA as a template. Then, with the participation of DNA polymerase and nucleotide substrate, the DNA was amplified to a sufficient number for structural and functional analysis.

    Scientists use PCR technology to amplify a small amount of RNA in the sample into deoxyribonucleic acid (DNA) and replicate until COVID-19(if present) is detected.

    What should we pay attention to before doing nucleic acid test？

    1.Try not to eat 2 hours before sampling to avoid vomiting.

    2. Do not smoke, drink, drink water or chew gum 30 minutes before sampling.

    3. The tester needs to wear a mask correctly and remove the mask before testing;

    4. Wear it immediately after testing, and prepare a spare mask for easy replacement at any time after pollution.

    5.When you feel sour and itchy in your nose or sneeze, you can immediately cover it with a paper towel or elbow.

    Before collecting nasopharyngeal swabs and before being tested, the collection personnel shall be informed whether they have relevant past medical history. For example, history of nasal surgery, history of deviation of nasal septum, blood diseases, throat diseases, etc

    How long will we know the pcr test results？

    The test results of sampling in the morning are generally available from 18:00 to 19:00.

    The detection results of afternoon sampling can generally be queried around 22:00.

    What’s the difference between the PCR and antigen tests for COVID-19?

    Polymerase chain reaction (PCR): PCR is the most reliable and accurate method to detect active infection. PCR tests usually give results in a few hours.

    Antigen detection: detect protein fragments on the surface of covid-19. Antigen detection usually takes only 15 to 30 minutes.

    How can we prevent COVID-19?

    Firstly, keep good personal hygiene habits, wear masks when going out, wash hands frequently, clean the room, and wipe the floor and other furniture with disinfectant. Second, disinfect your hands with 75% alcohol every time you go out and go home, reduce the number of times you go out, and wear qualified masks. And the diet should also be light, do a good job of physical exercise and enhance personal physique.

    Most importantly, if our conditions permit, we should vaccinate and conduct multiple nucleic acid tests.

    If our Nasal swab pcr test results are positive,what should we do?

    If our test results are positive, don’t be afraid. We should wear masks and go to the nearby Hospital for follow-up in time.

    Repeat the nucleic acid test.Don’t be afraid and hide the test results. Timely medical treatment is the best choice.

    Cell medical (Dongguan) Co., Ltd. is a comprehensive high-tech biotechnology company and an OEM factory of flocking swabs in China.We support custom production,our factory produces flocked swabs, pharyngeal swabs, nasal swabs, oral swabs,Rapid Antigen Test Kit, virus sampling tubes, transportation media, sample preservation solution and nucleic acid extraction kit.

    Welcome to leave a message and consult us!

     

     

    What is a sterile swab

    What is a sterile swab？

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    Sterile swab is the full name of disposable sterile sampling swab. The sterile sampling swab does not contain or other live microorganisms. Common sterilization methods include autoclave, EOS (ethylene oxide gas) and γ-ray irradiation. In medicine, we usually use sterile swabs for biological sample collection.  Such as bacterial sample processing, COVID-19, nasal swab sampling, throat swab sampling, oral swab sampling, virological cell culture and so on are ideal ways.

    Swabs are sterile to maintain the integrity of the sample. Usually, there is a resealable sterile container or bag to preserve the integrity during processing and transportation to the laboratory. Many different people use sterile cotton swabs, including medical personnel, food manufacturers and crime scene investigators.

    Sterile swabs can be used to collect samples. The material, size and shape of the sterile swab head may be different, such as round or pointed. Traditional swab may be made of cotton fiber, polyester or calcium alginate.  Collection and publishing volumes are large and may be corrupted. Traditional swab laboratory staff also need to use scissors to cut, which is too troublesome and time-consuming.Our new flocked disposable sampling cotton swab greatly reduces the time cost and has the advantage of being easier to use than traditional swabs.

    The main advantages of our sterile flocked swab are as follows.

    1. It has extraordinary water absorption capacity and can increase the number of samples collected on the watch from 20% of the traditional sampling swab to 60%.

    2. The release rate of collected samples exceeds 90%, which ensures the high reliability of the results.

    3. Select different preservation solutions for different types of samples.

    4. The plastic rod has a unique fragile design, which is convenient for sample transportation.

    5. Ethylene oxide sterilization, irradiation sterilization and independent packaging.

    Sampling operation method.

    1.Nasopharyngeal swab sampling.

    Nasopharyngeal Sampling diagram

    Nasopharyngeal Sampling diagram

    ①Insert a sterile swab into the nostril of the patient,reaching the surface of the posterior nasopharynx.

    ②Swab over the surface of the posterior nasopharynx. Hold the swab in the nosefor15-30s and rotate it 3 times.

    ③Withdraw the sterile swab from the nasal cavity.

    ④Put virus sampling tube vertically.

    ⑤At the breaking point, disconnect the swab, discard the tail, and completely place the swab into the tube.

    ⑥Tighten the tube cap, mark it, put it into the biosafety bag and seal it.

    2.Oropharyngeal swab sampling

    Oropharyngeal Sampling diagram

    Oropharyngeal Sampling diagram

    ① Keep the head slightly tilted, the mouth open, and the pharyngeal tonsils on both sides exposed.

    ②The sampling personnel shall wipe the root of the tongue with a swab. Wipe the pharyngeal tonsils on both sides of the subject back and forth with a strong force for at least 3 times, and then wipe the posterior pharyngeal wall up and down for at least 3 times.

    ③Put the sampled swab into the virus sampling tube, break the swab rod at the mouth of the tube cover and completely put it into the tube.

    ④Tighten the tube cap, mark it, put it into the biosafety bag and seal it.

    ⑤Submit for inspection in time.

    Anal swab sampling

    Anal Sampling diagram

    Anal Sampling diagram

    ①The patient is in the knee chest position or lateral position.

    ② Gently insert a sterile cotton swab into the anus for 3cm-5cm, and then gently rotate and pull it out.

    ③Immediately put it into a 15ml outer screw cap sampling tube with 3ml-5ml virus preservation solution.

    ④Fold off the tail of the cotton swab, tighten the sealing cover, and then submit it for inspection in time.

    ⑤In order to avoid embarrassment of the examinee, anal swab sampling is generally conducted in a more private place, and the operation requirements of anal swab sampling are more strict. Generally, there will be no obvious discomfort after anal swab sampling.