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What is Virus Sampling Tube

What is Virus Sampling Tube

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Since the outbreak of COVID-19 pneumonia, through multiple rounds of new coronavirus nucleic acid tests on personnel in relevant areas.

A virus sampling tube is a set of centrifuge tubes for sampling and transportation of influenza virus (including COVID-19), hand, foot and mouth virus and other viruses in the microbial sampling transportation tube.

Also known as the specimen transport tube, the virus transport medium has now become a commercial product.

Disposable sampling tube kit

The disposable sampling tube consists of a swab + sampling tube + virus preservation solution.

That is flocked swab, plastic virus sampling tube and virus transport medium.VTM

We have been able to quickly and effectively identify infected persons and treat them in a timely manner.

At the same time, we can quickly divide risk management areas and effectively Prevent the further spread of the epidemic.

The high efficiency and accuracy of the nucleic acid Swab Test is the “gold standard” for the diagnosis of new coronary pneumonia.

VTM KIST Virus Sampling Tube

Disposable virus sampler

Disposable sampling tube manufacturer Cell medical

In order to support the epidemic prevention work, Cell medical (Dongguan) Co., Ltd. has launched a single-use sampling tube.

Which meets the requirements of the COVID-19 virus nucleic acid sampling and testing technical specifications issued by the country.

VTM kits are suitable for the collection, transportation and storage of throat swab or nasal swab samples.

At the same time, our products have a variety of models and specifications, which can be used with multi-planting wool swabs, which are suitable for use by medical units at all levels.

At the same time, the kit contains a biosafety bag for the preservation and transfer of specimens after collection to prevent Accidental leakage, etc., improving the safety of use.

 

Do a good Virus Swab Test,Epidemic is not over yet

Do a good Virus Swab Test,Epidemic is not over yet

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Do a good Virus Swab Test, Epidemic is not over yet.

Winter has arrived, affected by the new crown pneumonia epidemic in Europe. Such as Cuba, the Netherlands, Germany, the United Kingdom, etc., the epidemic is continuing. More and more European countries have recently stopped restarting and tightening anti-epidemic measures. After the outbreak, European experience proved that: “Dynamic zero adjustment has been mastered” . “continuous changes in temperature rise have been controlled.” Advantages and captures “return to zero” and “constantly switch between limiting adjustments”.

COVID-19 mutant virus Omi Kiron strain has spread to all provinces in South Africa.

South African President Ramaphosa said on the 28th that in the next few weeks.

There may be a fourth wave of new crown epidemics in South Africa.

And the public should do a new crown virus sampling swab test as soon as possible and get the new crown vaccine.
In the evening, Ramafosa said in a televised speech that in the past 7 days.

The number of confirmed cases of the new crown in South Africa has risen to an average of 1,600 per day, while the positive rate of new crown virus testing in South Africa has risen from approximately 2% to 9% in the previous week.

If the newly emerging confirmed cases continue to rise, South Africa may enter the fourth wave in the next few weeks.
Lamafosa said that the recently emerged mutated virus, Omi Keron virus, has spread to all provinces in South Africa.

At present, immunization is still the most important means of protecting oneself and others.

The emergence of the Omi Keron virus has sounded a wake-up call for the whole world.

Several countries, including South Africa, have recently reported confirmed cases of Ome Keron, and the World Health Organization listed the virus as a “variety of concern” on the 26th.

Nasopharyngeal swab for covid

Do a good Virus Swab Test,Epidemic is not over yet

Do a good Virus Swab Test, Epidemic is not over yet

About the European epidemic

European experts believe that the recent occurrence of the “epicenter” of the global epidemic in Europe is related to factors such as disease awareness, premature “unblocking”, and insufficient vaccines. The most critical factor-the effect of excessive pendulum movement, which swings from side to side at a strict angle. We must do a good job in the swap test of the COVID-19.

Herd immunity？

Doing a good job of a virus swab test, testing and vaccination is the top priority.

At the beginning of the disease outbreak, Patrick Valence, the chief scientific adviser of the United Kingdom, put forward the theory of “herd immunity”. Now in the world, the EU has completed 7% of the population protection range of about 6%, and still cannot solve the fourth wave of epidemics.

The proliferation of medical resources film festivals appeared. The incidence is higher in the northern region. 79% of people abroad have received local injections. The number of the above cases has reached 9.8 million, and the proportion of the British population is 14.6%. New highs since the beginning of winter, the peaks of the virus wall in various states have taken turns, and no emergencies have occurred. The theory of “in the iron face, group immunity” has broken down.

“Coexisting with the Virus” Nonsense.

Mistakes in the fight against the virus continue to disrupt growth and even increase risks. From the beginning of the new century, the “labor shortage” and supply chain crisis in many countries can end this. Over the past year or so, Europe has had to return and fall in the policy choices of “severe cold and epidemic prevention” and “economic recovery”. The vicious circle of “high control-unraveling-epidemic rebound-re-management”.

However, as the life expectancy of relevant countries or regions has dropped significantly, the problems that gradually emerge after the outbreak of the disease will have a more severe and instantaneous impact.
It has achieved great success by adhering to the goal of zero-clearing the epidemic. Even eager to announce “coexistence with the virus”, trying to adopt a third response method under the situation of repeated zero-sum dynamics.
But, there will be such a road there? The result of choosing to “coexist with the virus” at this stage is nothing more than harming more people’s lives.

Learn more about virus tests, welcome to click：https://swabmanufacturer.com/

Viral Transport Kits | Viral Transport Medium | VTM Kit

Viral Transport Kits | Viral Transport Medium | VTM Kit

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Viral Transport Kits | Viral Transport Medium | VTM Kit

Winter is coming, the cold current is coming, the goose feather like snow is flying all over the sky, and the whole world is wearing a white coat.

Many customers once again throw this “soul question” to us. “Can your Viral Transport Kits adapt to the cold environment?”

He answer is yes. Let’s follow Cell medical Dr. Li for experimental verification!

Experimental purpose

Verify that the Viral Transport Medium of cell medical (Dongguan) Co., Ltd. has no significant impact on its service performance after low-temperature freezing and thawing.

Experimental scheme

Simulate the process of low-temperature freezing during transportation, place the sampling tube in a low-temperature environment for 24 hours, and then melt at room temperature. The sampling tube products stored normally without freezing and thawing were used as the control. Each group was added with virus mixture, stored at 4 ℃ for 24 hours, and then bathed in water at 56 ℃ for 30 minutes for nucleic acid extraction and nucleic acid detection. Set 2 repetitions for each group.

1. Low temperature icing simulation: set two low temperature gradients and place the sampling tube in two low temperature environments of – 20 ℃ and – 80 ℃ respectively.

2. Mixing of virus solution: We used two different concentrations of virus solutions, representing high and low concentration gradients respectively. The copy numbers of virus solution were 106 copies / ml and 104 copies / ml, respectively. Simulate the actual sampling process, add the virus solution in the volume ratio of 1:10 to the sampling solution, and the virus solution is 100 μ 50. 1ml of sampling solution and add 100 ml to it μ L of oral liquid.

3. After mixing each group, place it in 4 ℃ environment for 24 hours and inactivate it in 56 ℃ water bath for 30 minutes.

4. Extract the nucleic acid of each group of virus, and detect the virus content of each group by RT qPCR. The CT value is used as the basis for result evaluation. The higher the CT value, the higher the detected concentration.

5. The experimental groups are as follows:

Main experimental materials

  •   Sampling solution: virus preservation solution produced by cell medical (Dongguan) Co., Ltd.
  •  virus diluent: the virus isolation serum-free medium of Cell medical was used for gradient dilution.
  •   virus for verification: porcine epidemic diarrhea virus. Its structure and genome size are similar to those of New Coronavirus.
  •   nucleic acid extraction reagent: non cleavage nucleic acid extraction reagent (magnetic bead method).

Viral Transport Kits Experimental results.

1. Product appearance.

the color is consistent without precipitation.

2. Performance evaluation results

amplification curve.

A. amplification curve of each experimental group and negative control group:

The groups with earlier peaks are the amplification curves of high concentration groups, and the groups with later peaks are the amplification curves of low concentration groups.

Empirical conclusion.

The Viral Transport Medium products of Cell medical (Dongguan) Co. ,Ltd. will not affect its service performance after one-time freezing and thawing.

And can fully meet the requirements of low-temperature transportation and low-temperature preservation of virus samples.

Viral Transport Kits:composed of flocked swabs and test tubes with Viral Transport Medium(VTM)

 

Virus Transport Medium

What is the Virus Transport Medium and What is Its Role?

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What is the Virus Transport Medium and What is Its Role?

a medium used for the collection, transport and preservation of pathogenic bacteria or microbial samples. Including liquid and solid.

Generally,for the transportation and preservation of clinical samples.

It is used by medical units for pre-treatment of pathogenicity test to obtain pathogenic bacteria samples.

  Inspection principle

Different formulations adopted by different types of transport media can improve and enhance the viability of corresponding microorganisms in the samples.so as to facilitate the short-term preservation of the samples during transport.

Virus sampling tube Virus Transport Medium

Virus sampling tube_Virus Transport Medium

 Method of use

1. Before sampling, mark the relevant sample information on the label of the sampling tube.

2. Sample with corresponding sampling swab.

3. After sampling, quickly put the swab into the sampling tube.

4. Break the swab at the breaking point of the swab and discard the tail.

5. Tighten the pipe cover. Then put the sampling tube containing the sample into the biosafety bag and submit it for inspection in time.

  Features

1. Cell medical’s virus transport medium can preserve almost all cell morphology, structure and quantity.

2. The remaining cells after production can be further directly tested for HPV, DNA, chlamydia, gonorrhea, immunohistochemical tissue test and other tests.

eliminate most bacteria and microbial activities within 3 and 15 minutes. Avoid possible injury to medical staff.

VTM Collection Kits

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VTM Collection Kits

All VTM Collection Kits are currently in stock and ready to ship. Available with or without Phenol Red. Please contact our Customer Service Team for an official quote or to inquire about quantities of 50K or more.

Safely collect, handle and transport clinical specimens with our quality VTM kits, viral transport mediums, swabs and biohazard specimen bags.

Proper specimen collection and transport plays a critical role in laboratory diagnosis of infectious diseases associated with viruses. The VTM Collection Kits offered by Cell medical allow for the collection, preservation and transport of viruses, such as coronavirus (COVID-19). All VTM Collection Kits are made in the China and are manufactured according to CDC protocols for specimen collection and transport. Please note that products are available for research and clinical laboratories only.

Cell medical is  viral transport medium and flocked swab collection kit manufacturer. Please inquire for bulk pricing and larger case packs.

Transport medium: Made in an injection manufacturing facility, GMP(Good Manufacturing Pratice) product

Tube: E-Beam sterilization

Swab: E-Beam sterilization

High virus preservation and recovery rate, high bacterial culture

Corona Virus(Human) Recovery test: 3days, 95~110% Recovery rate

Chlamydia, Mycoplasma and Unreaplasma Recovery test

VTM Viral Transport Medium NP Kit – 3ml Vial

Contains one CELL VTM 3ml vial and one Nasal/Oropharyngeal Swab.
Approved for Emergency Use Authorization (EUA) under the policy outlined in Section IV.C of the FDA’s COVID-19 Transport Media Policy

NP SWAB AND OP SWAB BY SWABMUNUFACTURER

 

Viral Transport Medium China

Viral Transport Medium China Manufacturer

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Viral Transport Medium China manufacturer-Virus sampling tube

Viral Transport Medium is also called virus sampling tubes.

Cell medical not only produces sampling swabs, but also virus sampling tubes, general bacterial sampling tubes and sterile normal saline sampling tubes,Virus delivery medium swab, disposable sampling swab.

The factory is located in Dongguan and belongs to a high-tech enterprise.

Our virus sampling tubes are exported to developed countries in Europe and America and more than 100 countries and regions around the world.

The production capacity of  Viral Transport Medium is very strong, with a daily output of 400000 sets of virus sampling tubes.

Viral Transport Medium Package specification

2ml, 5ml, 10ml, etc.

Intended use

For the transportation and storage of clinical samples.

Inspection principle

Hank’s balanced salt to build a neutral environment;

As a protein stabilizer, bovine serum albumin and Hank’s equilibrium salt increase the survival time and infection stability of the virus;

Antibiotics have broad-spectrum antibacterial effect;

Phenol red is an acid-base indicator.

The pH value of the discolouration area is 6.6 (yellow) ～ 8.0 (red), and it is red when the pH value is 7.2 ～ 7.4.

Limitations of test methods

For the transportation and storage of virus samples.

Product performance index

Appearance: the sampling liquid in the tube is red, transparent and no precipitation liquid;

PH value: when stored at 25 ℃, the pH value is 7.2 ~ 7.4;

Loading capacity: no less than the marked capacity;

Stability: the product shall maintain the stability of various properties within one month beyond the validity period.

Sample requirements

The samples shall be transported to the corresponding laboratory within 2 working days after collection, and the storage temperature shall be 2-8 ℃; If it cannot be delivered to the laboratory within 48h, it shall be stored at – 70 ℃ or below, and the collected samples shall be delivered to the corresponding laboratory within 1 week. Specimens should avoid repeated freezing and thawing.

Main components

Hank’s equilibrium salt, phenol red, bovine serum albumin, gentamicin, polymyxin B, cryoprotectant, etc.

Storage conditions and validity period

Storage condition: 5-25 ℃, validity: 12 months

Viral Transport Medium China Manufacturer

Viral Transport Medium China Manufacturer

Usage

1. Collect samples with a sampling swab according to the sampling requirements.

2. Place the sampling flocked swab after collecting the sample into the sterile sampling tube.

3. Break the swab at the easily broken cut-off point of the sampling swab rod and discard the tail.

4. Tighten the sterile sampling tube cover.

5. Label the sterile sampling tube with information as required.

6. The samples shall be transported to the corresponding influenza monitoring network laboratory within 2 working days after collection, and the storage temperature shall be 2-8 ℃; If it cannot be sent to the laboratory within 48h, it shall be stored at – 70 ℃ or below, and the collected samples shall be sent to the corresponding network laboratory within 1 week. Specimens should avoid repeated freezing and thawing.

7. Specific sampling methods can refer to the following:

1) Pharyngeal swab specimen: use a special sampling swab to wipe the posterior pharyngeal wall and tonsils on both sides with moderate force, and avoid touching the tongue; Quickly immerse the swab head into the sampling solution, break the swab at the easily broken cut-off point of the sampling swab rod, and discard the tail. (applicable to a sampling of this product)

2) Nasal swab specimen: use a special sampling swab, gently insert the swab head into the nasal palate in the nasal canal, stay for a moment, and then rotate and exit slowly; Quickly immerse the swab head into the sampling solution, break the swab at the easily broken cut-off point of the sampling swab rod, and discard the tail. (applicable to a sampling of this product)

Precautions

1. Forbidden to directly contact the sampled liquid with the collected personnel.

2. Forbidden to infiltrate the swab with sampling solution before sampling.

3. Viral Transport Medium is a disposable product.

Only used for the collection, transportation and preservation of clinical virus samples.

4. When collecting virus samples, professionals should strictly follow the sampling procedures;

5. The samples shall be transported to the corresponding laboratory within 2 working days after collection, and the storage temperature shall be 2 ~ 8 ℃;

Specimens should avoid repeated freezing and thawing.

References

1. WHO Global Influenza network monitoring program

viral transport medium tube

Viral Transport Medium Tube

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Viral Transport Medium Tube

Whether nucleic acid detection or virus detection, Viral Transport Medium Tube and Sampling Swab play a great role.

Sampling swab is used to collect virus samples, while virus preservation solution plays the role of preserving these virus samples.

Which not only ensures the integrity of nucleic acid virus, but also facilitates the later detection accuracy.

How many virus preservation solutions are there? What are the functions and differences?

At present, there are two kinds of virus preservation solutions on the market, namely inactivated virus preservation solution and non inactivated Viral Transport Medium.

Non-inactivated  Viral Transport Medium can protect the protein and nucleic acid of the virus.

Inactivated Viral Transport Medium usually contains the cleavage salt of inactivated virus to cleave the protein and protect the nucleic acid.

Specific uses of virus sampling tube:

1. The virus sampling tube is generally used for nucleic acid extraction and later virus isolation of influenza virus, hand, foot and mouth virus and rubella virus in clinical patients. The amount of liquid required is usually 35m or 5m. (with nucleic acid detection kit and spore culture medium)

2. For the collection and short-term transportation of avian influenza virus in the external environment. The amount of liquid required is usually 6ml.

3.  For daily monitoring and sampling of poultry, pigs and other animals. The amount of liquid required is usually 15m.

4. For short-term preservation of virus samples. (2-8 ℃ for 48 hours) for necessary cell culture.

5. Used for long-term preservation of virus samples (- 70 ℃ – 196 ℃ for one year) for necessary cell culture.

6. To collect clinical respiratory virus rapid detection kit.

7. To collect clinical samples of mycoplasma, chlamydia and Ureaplasma.

8. To transport tissue samples from the sampling site to the detection laboratory for necessary cell culture.

Viral Transport Medium Sample Collection Tube Specimen Collection Kit

Viral Transport Medium Sample Collection Tube Specimen Collection Kit

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Viral Transport Medium Sample Collection Tube Specimen Collection Kit

Viral Transport Medium Sample Collection Tube: for collection, transportation and storage of detection samples.

The virus sampling tube kits includs a flocked swab and a centrifugal tube containing virus preservation solution.

In terms of protection, there are protection requirements.

As the manufacturer of virus sampling tube is a complete set of centrifuge, it can be used for microbial sampling and transportation of influenza virus, hand, foot and mouth disease virus and other viruses.

Therefore, the disposable virus sampling tube is more safe and convenient.

After all, the disposable virus sampling tube is very convenient to use and has no pollution problem.

Product name

Virus preservation solution

Disposable virus sampling tube-Viral Transport Medium

Disposable virus sampling tube-Viral Transport Medium

Package specification

3ml / piece

Intended use

For the collection, storage and transportation of human nasopharyngeal virus samples.

Inspection principle

This product is a non-toxic sample preservation solution. It can inactivate infectious pathogens and avoid the risk of secondary infection during operation. This product can inactivate more than 99% of cells or viruses within 10 minutes at room temperature to release DNA or RNA of cells or viruses. At the same time, the effective components in the preservation solution can keep RNA at room temperature for 7 days without obvious degradation.

Main ingredients

Product name Specifications

Sample preservation solution 3ml / piece

storage conditions and validity period

Keep at 2-37 ℃ for 12 months.

sample requirements

After sampling with this product, stored at room temperature for 7 days.Stored for a long time at low temperature.

Viral Transport Medium Specimen Collection Kit usage

1. Before sampling, clean your hands, rinse your mouth with water for 1-2 times, remove food residues in your mouth, and swallow the remaining water in your mouth.

2. Tear off the outer package of the sampling swab.

3. Put the swab into the mouth and wipe it repeatedly at the medial buccal mucosa for more than 8-10 times until the nylon pile has been fully infiltrated by saliva (there should be no foreign matter such as food residue).

4. Open the sample collection tube, put the sampled swab into the preservation solution for wetting, and break the swab rod near the top.

5. Tighten the tube cap and complete the sample sampling.

precautions

1. Please check whether the package is intact before use. Do not use if damaged.

2. The collected swab samples can be stored at 2-37 ℃ for 7 days and – 40 ℃ for 12 months. In view of the individual differences of samples, the specific storage periods of different samples are different.

3. The nucleic acid of swab samples can be purified using commercial related kits.

Related products: disposable virus sampling tube, virus sampling tube, sampling tube

 

viral transport medium with swab

Viral Transport Medium with Swab

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viral transport medium with swab | COVID-19 sampling and collection kits

The epidemic has disrupted the whole world and affected many industries.

During the outbreak of  COVID-19, people were paying close attention to the epidemic situation while doing personal protection. Novel coronavirus pneumonia and influenza were the most frequent period of communication in winter and spring. The most important way to control the epidemic is to select new patients with new crown pneumonia from many respiratory patients.

About viral transport medium with swab.

Swabs and virus transport media are a transport and collection system for long-term freezing, transport and collection of clinical specimens containing viruses.

The main species of organisms usually transported through this medium include chlamydia, urea plasma organisms and mycoplasma.

With the outbreak of COVID-19 pandemic, swabs and virus transport mediums were used to freeze and collect SARS-CoV-19 virus samples.

Rub a swab on the skin or vesicles to collect virus samples. The collected samples are then sent to the laboratory with transport media.

Why do swabs need virus transport media？

After virus collection, the traditional method can not be detected by PCR immediately. The virus itself will quickly dissolve in vitro and affect subsequent tests.

viral transport medium with swab -vtm kits

viral transport medium with swab -vtm kits

Purpose of virus transport media:

1. For monitoring and sampling infectious pathogenic microorganisms by disease control departments and clinical departments. It is applicable to the sampling of influenza virus (ordinary influenza, highly pathogenic avian influenza, influenza A H1N1 virus, etc.), hand, foot and mouth virus and other types of viruses.

2.For the sampling of mycoplasma, chlamydia and Ureaplasma.

3.. To transport nasopharyngeal swab samples or tissue samples at specific parts from the sampling site to the detection laboratory for PCR extraction and detection.

4.To preserve nasopharyngeal swab samples or tissue samples at specific sites for necessary cell culture.

Precautions for sample transportation.

1. Inspection time and temperature control:

After samples sampling, for inspection as soon as possible and sent to the laboratory within 2 ~ 4H as far as possible.

Transport at 2 ~ 8 ℃, and the transport time shall not exceed 72h. If it exceeds 72h, stored and transported at – 70 ℃ or lower.

2. Viral transport medium transport container:

Virus transport tube：waterproof, damage proof, leak proof, high temperature (low temperature) and high pressure resistant. 
Tube body: biological hazard signs, warnings and reminders with relevant regulations. 
Transport container: three-layer packaging system, namely inner packaging, middle packaging and outer packaging.

3. Transportation in the hospital:

The specimen delivery personnel shall be provided with secondary protection and carry 75% ethanol with them.

4. Long distance transportation:

The transport package of 2019 ncov specimens belongs to class A, and the corresponding UN number is un2814. The transporter shall wear safety protection according to the requirements of class II protection, and carry 75% ethanol with him. Drivers wear surgical masks or N95 masks and transport them by special vehicles. If transported by air, the packaging shall also comply with the pi602 classified packaging requirements of ICAO document Doc9284-AN / 905 technical rules for safe air transport of dangerous goods. At least one specimen transporter and driver shall transport specimens at the same time, and monitoring facilities for specimen transport process shall be equipped.

Contact us

  • Cell medical (Dongguan) Co., Ltd.
  • skype:+86-18814476009
  • E-mail：flockedswabs@gmail.com
  • No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China
  • Web：www.swabmanufacturer.com/

 

COVID-19 Sample Collection and Transport kits

COVID-19 Sample Collection and Transport kits

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COVID-19 Sample Collection and Transport kits

Since the outbreak of the new crown, we have received many customer queries about which types of swabs, media and kits can be used for sample collection and transportation of COVID-19.

Cell medical (Dongguan) Co., Ltd. produce flocked swabs, VTM kits, virus transport medium, with multiple automatic production lines.

We support custom production, OEM and so on, and develop products with customers.

Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.

Our VTM kits are manufactured in accordance with CDC and FDA guidelines.

Now let’s learn about sample collection and transport kits.

COVID-19 Sample Collection and Transport kits

COVID-19 Sample Collection and Transport kits

How do we collect COVID-19 samples?

Specimen collection requirements

Personal protective equipment (PPE) requirements for sampling personnel: N95 and above protective masks, goggles, one-piece protective clothing, double-layer latex gloves and waterproof boots; In case of contact with the patient’s blood, body fluids, secretions or excreta, replace the outer latex gloves in time.

Specimen type

1. Upper respiratory tract specimens: including pharyngeal swab, nasal swab, nasopharyngeal extract, etc.

2. Lower respiratory tract specimens

3. Stool samples: Patients with diarrhea symptoms need to collect stool samples.

Specimen collection method

Sampling flocked swab_Sample Collection

Sampling flocked swab_Sample Collection

1. Pharyngeal swab: wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with 2 plastic rod swabs planted with pile at the same time, immerse the swab head into a tube containing 3ml virus preservation solution (isotonic salt solution, tissue culture solution or phosphate buffer can also be used), discard the tail, and tighten the tube cover.

2. Nasal swab: gently insert a plastic rod swab with a flocking nylon faucet into the nasopalatine part of the nasal canal, stay for a moment, and then slowly rotate and exit. Take another plastic rod swab with polypropylene fiber head and collect the other nostril in the same way. Immerse the above two swabs into the same tube containing 3ml sampling solution, discard the tail, and tighten the tube cover.

What kind of Media do you use to transport specimens?

Since the outbreak of COVID-19, the Virus Transport Medium has played a major role. Sample and nasal swabs are used to protect samples in the sample tube with virus preservation, which improves the accuracy of detection. Virus preservation solution is a solution for virus sampling, preservation and detection. What kinds of virus preservation solution are added to the virus sampling tube?

virus transport medium

virus transport medium

There are two types of virus preservation transport Media: inactivated and non inactivated.

The following briefly introduces several principles for the selection of virus preservation solution.

Non inactivated virus transport medium:

The non inactivated type does not contain lysate.

On the basis of Hank’s, BSA (bovine serum albumin) amino acids, vitamins and other nutrients required by the virus are added, which can maintain the activity of the virus in a wide temperature range and maintain the originality of the sample to the greatest extent.

For nucleic acid extraction and detection of the virus, virus culture and separation.

Characteristics of non inactivated preservation solution:

COVID-19 Sample Collection and Transport kits Use process

COVID-19 Sample Collection and Transport kits Use process

1. It can maintain virus activity for detection or virus isolation;

2. It has no inhibitory effect on subsequent nucleic acid amplification;

3. stored in cold storage(at 2 ℃ ~ 8 ℃ ).

Inactivation virus transport medium:

Inactivation preservation solution can inactivate the virus in the sample by fully mixing the collected sample with virus lysate and virus nucleic acid preservation solution, and effectively ensure the integrity of virus nucleic acid in the sample.

About the preserved viral RNA samples: used in gene detection, enzyme-linked immunosorbent assay (ELISA), PCR detection and so on.

Inactivation preservation solution includes guanidine salt containing and guanidine salt free.

1. Guanidine salt

(1) Instantly cleaved and inactivated to reduce the risk of sampling;

(2) Stored stably at room temperature for 2 weeks;

(3) It can match most nucleic acid extraction reagents.

2. Non refined salt

(1) Inactivated instantaneously to reduce the virus sampling risk, and the virus may recover its infectivity after dilution;

(2) Stored stably at room temperature for 2 weeks;

(3) It can match most nucleic acid extraction reagents.

3. Direct expansion preservation solution

(1)Inactivated instantaneously to reduce the virus sampling risk, and the virus may recover its infectivity after dilution;

(2)Stored stably in cold storage for 24-48 hours;

(3) Directly added to the nucleic acid amplification system as a template;

(4) Match most nucleic acid extraction reagents and nucleic acid release agents.