Category Archives: flocked swabs

COVID-19 sampling tools

COVID-19 sampling tools help SARS-CoV-2 precise diagnosis!

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COVID-19 sampling tools help SARS-CoV-2 precise diagnosis!

Cell medical (Dongguan) Co.,Ltd. monthly production of millions of virus sampling swabs and VTM kits helps epidemic prevention and control.

What are the covid-19 sampling tools？

Flocked sampling Swabs,VTM Kits,Viral Transport Medium,VIRUS Collection & Transport Kits,Saliva collection kit,Disposable virus sampling tube,Op swabs,Np swabs,etc.

Why use sampling tools？

At present, the global New Coronavirus epidemic has reached a critical stage. The most reliable means of rapid diagnosis is still positive for nucleic acid detection. There has been a great breakthrough in the diagnosis of COVID-19, but clinicians, especially the laboratory doctors, still face the following serious problems:

1. Safety of specimens

The majority of “angels in white”, especially the laboratory doctors, are always facing the risk of virus infection.

Although there are various protective equipment, the risk of infection will increase if we can’t ensure the safety of specimens. Therefore, it is very important to ensure virus inactivation and the safety of collected samples.

2. False negative test results

The existence of false negative puzzles the clinic and affects the accuracy of diagnosis. The biggest problem is sample collection and preservation. The New Coronavirus infection prevention and control program (Fifth Edition) has been published to collect nasal swabs, throat swabs, sputum, lower respiratory tract secretions, blood and feces. The incorrect way of collecting and storing samples will lead to the degradation of viral RNA and directly lead to the false negative test results.

Cell medical (Dongguan) Co., Ltd. provides the perfect solution for sampling tools.

√. Cell medical provides samples of swabs collected from various viral types including New Coronavirus (covid-19) and provides a perfect solution.

√ .It can quickly inactivate viruses including New Coronavirus, influenza A virus and many other viruses.

√ .preservation solution: preserve RNA to prevent degradation.Disposable virus sampling tube.

√ .safety guarantee: vertical design, sampling and preservation in one step to reduce personnel exposure.

Sampling steps of new virus collection tube

1) The collection personnel shall be qualified through biosafety training and have corresponding experimental skills. Requirements for protective packaging of sampling personnel: N95 and above protective masks, goggles, one-piece protective clothing, double-layer latex gloves, waterproof boot covers, etc. in case of direct contact with the specimen, the protective equipment shall be replaced in time.

2) Sample type: pharyngeal swab and nasal swab.

nasal swab_COVID-19 sampling tools

nasal swab_COVID-19 sampling tools

Pharyngeal swab collection method: wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with a plastic rod swab with polypropylene fiber head

Nasal swab collection method: gently insert a plastic rod swab with polypropylene fiber head into the nasopalatine part of the nasal canal, stay for a moment, and then slowly rotate and exit. Take another plastic rod swab with polypropylene fiber head and collect the other nostril wall in the same way.

3) Specimen preservation: immerse the swab head into the tube containing virus preservation solution, discard the handle along the folding point of the sampling swab, and tighten the tube cover.

Notes

The tube containing virus preservation solution another name isvirus sampling tube.

Virus preservation solution is also called virus transport medium.VTM.

 

 

What is genetic testing?

What is genetic testing?

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What is genetic testing?

Definition of gene

What we usually call genes is a collection of genes in human cells, called the genome.

Every cell in our body has a complete genome. Inherited from parents and passed on to children.

Genes make people different from animals, make families different from families, and brothers and sisters are similar but different.

These subtle differences often do not exceed 0.5% of genes.

But determine different appearance, personality, genetic characteristics, and our adaptation to the external environment.

Why genetic testing?

It must be noted that various human traits and diseases are the result of the joint action of genes and environment .

Genes only play a partial role in traits, and whether gene expression is also greatly affected by the internal and external environment.

Therefore, gene test reports can not be directly used as the basis for clinical treatment, and it is not advisable to judge the role of genes without individual environment.

On this premise, the gene test report still has a certain reference significance for understanding various characteristics of the human body.

Understand that they carry hereditary pathogenic genes

We can know whether we carry pathogenic genes through genetic testing. For example, people with a family history of genetic diseases, cancer and hypertension. According to the test results, adjust dietary health care and living habits to avoid the occurrence of diseases. If both pregnant couples carry the pathogenic gene of recessive disease at the same time. They can pay attention and conduct further investigation when preparing for delivery.

Select drugs correctly to avoid drug waste and adverse drug reactions

Due to genetic differences, individuals will respond differently to drugs. When taking the same kind of drugs, some people will feel very effective, while others will not only be ineffective, but also have toxic and side effects. When some people use certain drugs, they may also have drug allergy. Through gene testing, we can know everyone’s gene situation, which may play a guiding role in drug use to a certain extent. Again, the drug test report can not be directly used in clinic. The drug selection and use in the treatment of diseases need to follow the doctor’s advice.

Provide scientific health management

Many adverse environmental factors  and bad living habits  will make the human body more prone to diseases. People can understand the occurrence tendency of individuals in different diseases through genetic testing, and make corresponding life adjustment or intervention in order to reduce the risk and delay the occurrence of diseases.

What is the principle of gene testing?

To understand the principle of gene detection, we need to understand the following questions:

Reasons for gene testing

Each person’s human nucleus has 23 pairs of chromosomes, which are composed of DNA and ribosomal proteins. All chromosomes have 3.16 billion base pairs.

When the same base pair position changes (this situation is generally called single nucleotide polymorphism, or SNP), which has a certain impact on human genetics and health, this SNP is the locus in the gene report. At present, there are nearly more than 100 million known or named SNPs.  Most of the differences between human DNA are SNP differences, which is about 0.5% of the total base pairs of human DNA. A large part of gene detection is based on SNP.

Samples for genetic testing

Normal human samples required for gene testing include saliva sampling and blood sampling. For different examination items, excreta sampling and cell slice sampling are also included.

Methods of gene testing

There are several ways to measure related base pair differences (SNPs): PCR, chip, exon, and genome-wide. The number of SNPs detected is dozens, hundreds of thousands, tens of thousands, and all SNPs.

Disposable DNA sampling flocked swab

Oral DNA sampling swab_flocked swab cm-98000

Oral DNA sampling swab_flocked swab cm-98000

1.Specially designed for micro DNA samples at the crime scene, especially for micro samples such as sweat, semen, blood, dandruff, oral cells, etc.

2. It can quickly absorb a small amount of samples and has high release aging rate.

3. The front end is sharp enough to extract cells from the victim or suspect’s fingernails.

4. The swab has a breakpoint, which is convenient for the automatic extraction workstation to extract samples.

5. The whole swab is packed in a transparent plastic tube to avoid biological pollution.

Who can do genetic testing?

① People with family disease history;

② People with bad living habits;

③ People exposed to chemical pollution, heavy metal pollution, nuclear pollution and toxic and harmful substances for a long time;

④ Friends who pay attention to health;

Hello!

When many diseases are diagnosed, it’s too late. Do a good job in disease prevention to make us healthy for a longer time!

 

 

 

COVID-19 Testing: Method of collecting virus swab samples

COVID-19 Testing: Method of collecting virus swab samples

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COVID-19 Testing: virus swab

According to New Coronavirus’s pneumonia diagnosis and treatment plan (trial version fourth), to diagnose suspected cases of New Coronavirus pneumonia, we need one of the following etiological evidence:

1. Respiratory tract specimens or blood samples were detected by real-time fluorescence RT-PCR for New Coronavirus nucleic acid positive.

2. Novel coronavirus is highly homologous to respiratory tract specimens or blood samples.

Therefore, novel coronavirus pneumonia is an indispensable part of the specimen collection. So, where to collect specimens? How to collect specimens? How can medical staff protect themselves during collection? What should I do after collection?

How to obtain a virus swab specimen?

We all know that the collection method of virus swab specimen is basically to use the swab to sample the infected place or part of the virus, and then put it into the virus sampling tube containing preservation solution without touching other places, so as to conduct virus detection later.

Specimen type:

1. Upper respiratory tract specimens: including pharyngeal swab, nasal swab, nasopharyngeal extract, etc.

2. Lower respiratory tract specimens: including deep expectoration fluid, respiratory tract extract, bronchial lavage fluid, alveolar lavage fluid and lung tissue biopsy specimens.

3. Blood samples: try to collect anticoagulants in the acute stage within 7 days after onset. The collection volume is 5 ml, preferably fasting blood.

4. Serum samples: try to collect double serum in acute stage and recovery stage.

5. Conjunctival specimens: conjunctival swab specimens shall be collected for cases with ocular infection symptoms.

6. Stool samples: Patients with diarrhea symptoms need to collect stool samples.

COVID-19 Testing: Common virus swab sampling methods

Methods of throat swab collection

Inform the patient of the purpose of examination, collection method and collection time. It is best to collect specimens before using antibiotics.

Objective: To study the clinical effect of the method

Secretions were collected from pharynx or tonsil for bacterial culture or virus isolation to assist in clinical diagnosis and treatment.

Prepare sampling supplies:

Disposable sampling swab, virus sampling tube, tongue depressor and biological bag

Operation method:

(1) Ask the patient to sit down, tilt his head back, open his mouth and make a “ah ~” sound

(2) The sampling personnel shall fix the patient’s tongue with a tongue depressor, and use a disposable pharyngeal swab to pass through the root of the tongue to the posterior pharyngeal wall, tonsil recess and lateral wall;

(3) Repeatedly wipe 3-5 times to collect mucous membrane cells or sputum

(4) . gently remove the swab to avoid touching the tongue, the suspension, the oral mucosa and saliva

(5) Put the swab into the virus sampling tube gently and vertically. The sampling personnel shall disconnect the tail of the flocking swab and leave the tail of the swab completely in the tube.Be careful that the swab does not touch the wall of the tube.

(6) . tighten the cap, mark it, and seal it in the biosafety bag.

(7) The sampling personnel shall send the samples to the laboratory for testing in time. If the sampling personnel cannot send the samples for inspection in time, they shall store them at low temperature

Methods: nasal swabs were collected

Nasopharyngeal swab_COVID-19 Testing: Method of collecting virus swab samples

Nasopharyngeal swab_COVID-19 Testing: Method of collecting virus swab samples

(1) Ask the patient to sit down with his head slightly back and remove the secretion from the front nostril first;

(2)measure the distance from nostril to ear base with swab and mark with finger

(3)gently insert the swab into the nostril until it is marked or stopped when there is obstruction. Let the swab stay in the nose for 15-30 seconds, and then rotate it gently three times.

(4) slowly pull out the nasal swab and quickly put it into the virus sampling tube. Then break the swab, leave its tail and place it completely in the tube.

(5) tighten the cap, mark it, and seal it in the biosafety bag.

(6) If we need to collect from both nostrils, we should use a swab.

(7) It should be sent to the laboratory for testing in time. If it can not be sent for inspection in time, it should be stored at low temperature.

Generally speaking, COVID-19 testing: the main methods of collecting virus swabs are nasopharyngeal sampling and oropharyngeal sampling.

 

Is COVID-19 pneumonia more infectious than SARS?

The effectiveness of infectious disease transmission depends on its basic regeneration index. Novel coronavirus pneumonia is more infectious than SARS at present, with a basic regeneration index of SARS higher than that of SARS.

The novel coronavirus pneumonia is a new disease, and the population is generally immune. Everyone is susceptible and susceptible to infection. The incubation period of COVID-19 also has the ability to transmit, and it has longer infection time. The novel coronavirus pneumonia novel coronavirus pneumonia is also typical of the clinical symptoms, and asymptomatic infections are also contagious, so the infection of new crown pneumonia is higher than SARS.

About us

Cell medical (Dongguan) Co., Ltd.  is a swabs manufacturer. We produce and sell virus sampling related products. Such as virus sampling flocked swabs, VTM kits and virus transport media, and have multiple automatic production lines.  We support customized production, OEM, etc., and develop products with customers.

 

Disposable virus sampling kits

Disposable virus sampling kits

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Disposable virus sampling kits

Cell medical (Dongguan) Co., Ltd. _China swab manufacturer.Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.

We produce flocked swabs, VTM kits, virus transport medium, Disposable virus sampling kits,with multiple automatic production lines.We support custom production, OEM and so on, and develop products with customers.

Email:flockedswabs@gmail.com

Nasopharyngeal Swab & Oropharyngeal Swab

Item No.# Description Sterilization
CM-98000 Oropharyngeal Specimen Collection flocked Swab, Single swab packaging, Sterile,Breakpoint 30mm EO
CM-96000 Nasopharyngeal Specimen Collection flocked Swab, Single swab packaging, Sterile,Breakpoint 80mm EO
CM-93050 Throat Specimen Collection flocked Swab, Single swab packaging, Sterile,Breakpoint 80mm EO
CM-96000N Nasopharyngeal Specimen Collection flocked Swab, Single swab packaging, Sterile,Breakpoint 90mm EO
CM-98000T Medical Flocked Swab With Test Tube, with Individually Wrapped and Sterile Buccal（oral)  Swabs EO
CM-93050T Specimen Collection Swab tube, Individual Sterile Package Oropharyngeal Swabs EO
CM-96000T Nasal Sample Collection Kits,flocked Swab With Test Tube,ndividually Wrapped and Sterile EO

Ethylene oxide sterilization, irradiation sterilization and independent packaging.

Supply Ability:500,000-1,000,000 Pieces per Month

Disposable sampling swab application

The top of the flocked swab is made of nylon fiber. The nylon fiber is successively attached to the surface of the medical ABS rod through the electrostatic field. The layer is completely parallel and perpendicular to the surface of the internal rod for DNA collection.

The collection system adopts nylon flocked swab, which is non-toxic to microorganisms and can maximize the collection and release of samples.

A large number of clinical experiments show that compared with ordinary sterile cotton swab, nylon flocking swab has better effect on the collection and transportation of clinical microbial samples

This is especially true for those specimens that cannot be submitted for examination in time and placed for too long.

Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.

Product features

1.Non invasive,Non-irritating；

2.Outstanding sample collection and release ability;

3.Rapid Absorption;

4. Increased Assay Sensitivity;

5.Perpendicular Nylon Fibers;

6.Certified Free Of Inhibitors And Interference.

Our FDA & CE certificate can ensure effective and legal customs clearance in your country.

VTM (Viral Transport Medium):Including inactivated and non inactivated.

Quality Certification:MSDS,CE,ISO

Our virus transport medium is based on Hanks equilibrium salt solution (HbSS).

It contains calcium and magnesium, heat inactivated fetal bovine serum, gentamicin and amphotericin B.

The composition and preparation of virus vectors follow the recommendations of who and CDC.

The product is provided in a sterile 13 ml flat bottom tube with or without swabs, providing the greatest range of possibilities for collecting samples.

Inactivated Viral Transport Medium(ITM).

This product is designed for the inactivation of a variety of viruses, such as clinical 2019-ncov, influenza, avian influenza, hand-foot-mouth, measles virus, norovirus, rotavirus and other virus specimens, and for the transportation of the virus nucleic acids. It is compatible with various viral nucleic acid extraction kits available on the market and can achieve seamless connection with downstream nucleic acid extraction.

1. It can not only inactivate the virus, but also prevent nucleic acid degradation.

2. Room temperature transmission

3. The samples can be stored at room temperature for 20 days.

4. It is used to collect, store and transport viruses, chlamydia, mycoplasma and Ureaplasma.

Model Specification
VTM-B-CM010120S 2With Nasal flocked Swab& Throat Swab ;  1ml inactivated medium ;2ml tube
VTM-B-CM010111S With Nasal Swab ; 1ml inactivated medium ;2ml tube
VTM-B-CM010112S With Throat Swab ; 1ml inactivated medium ;2ml tube
VTM-B-CM020220S 2With Nasal Swab& Throat Swab ;  2ml inactivated medium ;5ml tube
VTM-B-CM020211S With Nasal Swab ; 2ml inactivated medium ;5ml tube
VTM-B-CM020212S With Throat Swab ; 2ml inactivated medium ;5ml tube
VTM-B-CM020320S 2With Nasal Swab& Throat Swab ;  3ml inactivated medium ;5ml tube
VTM-B-CM020311S With Nasal Swab ; 3ml inactivated medium ;5ml tube
VTM-B-CM020312S With Throat Swab ; 3ml inactivated medium ;5ml tube
VTM-B-CM040320S 2With Nasal Swab& Throat Swab ;  3ml inactivated medium ;10ml tube
VTM-B-CM040311S With Nasal Swab ; 3ml inactivated medium ;10ml tube
VTM-B-CM040312S With Throat Swab ; 3ml inactivated medium ;10ml tube
VTM-B-CM040520S 2With Nasal Swab& Throat Swab ; 5ml inactivated medium ;10ml tube
VTM-B-CM040511S With Nasal Swab ; 5ml inactivated medium ;10ml tube
VTM-B-CM040512S With Throat Swab ; 5ml inactivated medium ;10ml tube

Non inactivated Viral Transport Medium(ITM).

Application:Nucleic acid detection and virus isolation.

1. Low temperature non freezing storage, does not damage the shell of the virus, and is convenient for long-distance transportation.

2. Applicable to all kinds of swab samples, including oral swab, nasal swab, throat swab, etc

3. It can be used for H1N1 influenza virus and any other virus that can be sampled with a swab.

4. The viral nucleic acid can be extracted with the lentivirus DNAOUT or the lentivirus RNAOUT.

5. Antibiotics in the sampling solution can effectively prevent bacterial and fungal contamination.

6. Bovine serum albumin is added to the sampling solution, which can protect the virus sample and improve the separation rate.

Model Specification
VTM-A-CM010120S 2With Nasal flocked Swab& Throat Swab ;  1ml Non inactivated medium ;2ml tube
VTM-A-CM010111S With Nasal Swab ; 1ml Non inactivated medium ;2ml tube
VTM-A-CM010112S With Throat Swab ; 1ml Non inactivated medium ;2ml tube
VTM-A-CM020220S 2With Nasal Swab& Throat Swab ;  2ml Non inactivated medium ;5ml tube
VTM-A-CM020211S With Nasal Swab ; 2ml Non inactivated medium ;5ml tube
VTM-A-CM020212S With Throat Swab ; 2ml Non inactivated medium ;5ml tube
VTM-A-CM020320S 2With Nasal Swab& Throat Swab ;  3ml Non inactivated medium ;5ml tube
VTM-A-CM020311S With Nasal Swab ; 3ml Non inactivated medium ;5ml tube
VTM-A-CM020312S With Throat Swab ; 3ml Non inactivated medium ;5ml tube
VTM-A-CM040320S 2With Nasal Swab& Throat Swab ;  3ml Non inactivated medium ;10ml tube
VTM-A-CM040311S With Nasal Swab ; 3ml Non inactivated medium ;10ml tube
VTM-A-CM040312S With Throat Swab ; 3ml Non inactivated medium ;10ml tube
VTM-A-CM040520S 2With Nasal Swab& Throat Swab ;  5ml Non inactivated medium ;10ml tube
VTM-A-CM040511S With Nasal Swab ; 5ml Non inactivated medium ;10ml tube
VTM-A-CM040512S With Throat flocked Swab ; 5ml Non inactivated medium ;10ml tube
Disposable virus sampling kits sampling process

Disposable virus sampling kits sampling process

Nasopharyngeal swab sampling_flocked swabs

Nasopharyngeal swab sampling_flocked swabs

Oropharyngeal swab sampling

Oropharyngeal swab sampling

Nasal swab for flu

Nasal swab for flu,Rapid antigen nasal or throat swab

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Nasal swab for flu_Rapid antigen (nasal or throat swab) test

Influenza is common in winter and spring. The clinical manifestations are mainly systemic poisoning symptoms such as high fever, fatigue, headache, cough and systemic muscle pain, while the respiratory symptoms are mild.

Influenza viruses are prone to mutation, infectious, susceptible to population and high incidence rate. They have caused many outbreaks in the world, and are important public health issues worldwide.

Why should we detect and prevent influenza?

According to the World Health Organization (WHO), seasonal influenza can cause 3-5 million severe cases and 290000-650000 deaths worldwide every year.

The researchers believe that children under the age of 5 are at high risk of severe influenza, but children under the age of 2 are at the highest risk, and infants under 6 months have the highest hospitalization rate and mortality.

Pregnant women, infants, the elderly, patients with chronic basic diseases and other high-risk groups have a higher risk of serious diseases and death after suffering from influenza.

Nasal swab for flu : common influenza test method

Nasopharyngeal sample collection

Nasopharyngeal sample collection

Influenza is an acute inflammation of the respiratory tract caused by the patient’s infection with influenza virus. The patient can have a series of symptoms such as cough, expectoration, fever, sore throat, stuffy nose, runny nose and general fatigue. In case of influenza, we should conduct relevant examinations in time to further clarify the diagnosis, including the following tests:

First, blood routine test, patients with influenza can promote the increase of lymphocytes. If combined with bacterial infection, there can be the increase of leukocytes and so on.

Second, scientists can culture throat swabs of influenza viruses. In throat swab culture, researchers can detect the related virus.

Third, influenza patients can do second-generation sequencing, including sputum second-generation sequencing, which can determine the type of virus infected.

Fourth, influenza patients can do the detection of respiratory pathogens to further clarify the pathogens of infection.

At present, the two most common influenza tests are rapid antigen diagnostic test  and influenza virus cell culture test. Both tests rely on Nasopharyngeal swabs, pharyngeal swabs, etc. for virus sampling. The most commonly used sampling swab is nylon flocking swab.

Nasal swab for flu:Application

It is suitable for the detection and sampling of COVID-19, influenza virus , hand foot mouth disease virus, COVID-19, measles rubella and other types of viruses.

About nasal swab sampling

Why use flocked nylon swabs?

flocked swabs_Cell medical (Dongguan) Co., Ltd.

flocked swabs_Cell medical (Dongguan) Co., Ltd.

Flocked nylon swab: excellent sample collection / release ability, release rate > 90%.

Conventional fiber swab: the sample is intercepted by the gap in the fiber mass. That is, the sample is penetrated, diluted and intercepted in the fiber.  Acquisition is slow and release is slower.

The sampling time of flocked swab is 3-5 seconds, while that of traditional swab is 15-120 seconds or even longer.

This caused pressure on the staff during the diagnosis process. Only by extracting samples faster can we complete the collection and analysis more efficiently.

Precautions for patients with nasopharyngeal swab:

① Please do not take antibiotics a few days before making nasal swabs;

② Do not use disinfectant mouthwash or smear the focus a few hours before nasal swab.

③ Do not eat, smoke or drink a few hours before doing nose swab.

Precautions for Sampler:

① . the sampler shall stand on the side of the patient for sampling.

② The patient is required to pull down the mask to expose only his nostrils. In case of sneezing reflex, the patient can cover it with elbows or paper towels.

If the sampler is not directly in front of the patient, the exposure risk will be lower.

③ If the patient’s turbinate is hypertrophic, the nasal tract is narrow, and the insertion of the nasal swab feels resistance. Try to change one side of the nasal cavity or directly change to oropharyngeal swab collection.

④ Those with nasal allergy symptoms are easy to induce sneezing. It is recommended to take oropharyngeal swab samples.

⑤ Remember to wear a protective mask / screen. For individuals who are highly suspected or basically diagnosed, or who have severe cough and hiccup, they should put on protective clothing.

⑥ After sampling, replace gloves, disinfect hands, and spray disinfectant where patients have touched.

Finally, we should pay attention to nasal swab for flu in special times. Remember to wear masks and personal hygiene when going out. Once you find the virus, please isolate it to avoid re transmission.

 

Nasal swab pcr test

Nasal swab pcr test

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Nasal swab pcr test COVID-19 Antigen Rapid Test Kit

Detection of COVID-19 by nasal swab PCR is an accurate and reliable method. Positive detection means you may have SARS-CoV-2. Negative detection means you may not have SARS-CoV-2 when testing.

In order to ensure your safety, if you have symptoms of COVID-19. Or people who have been exposed to the virus and tested positive should be tested.

What is the symptom of COVID-19?

If you have the following symptoms,if you have the following symptoms, please buy covid-19 antigen rapid test kit immediately or go to a nearby hospital for PCR test.

1.Fever;

2.General fatigue;

3.cough;

4.Dyspnea or even respiratory failure；

5.Nausea;

6.vomiting;

7.abdominal pain;

8.diarrhea;

Symptoms of New Coronavirus pneumonia are not necessarily typical, and some patients may not have clinical manifestations at the early stage of New Coronavirus infection. Then clinical manifestations will appear, that is, “asymptomatic infection” in incubation period.

In other words, try to avoid activities in places with crowd gathering, closed space and poor ventilation.

What is nasal swab PCR test？

Polymerase chain reaction (PCR) uses a piece of DNA as a template. Then, with the participation of DNA polymerase and nucleotide substrate, the DNA was amplified to a sufficient number for structural and functional analysis.

Scientists use PCR technology to amplify a small amount of RNA in the sample into deoxyribonucleic acid (DNA) and replicate until COVID-19(if present) is detected.

What should we pay attention to before doing nucleic acid test？

1.Try not to eat 2 hours before sampling to avoid vomiting.

2. Do not smoke, drink, drink water or chew gum 30 minutes before sampling.

3. The tester needs to wear a mask correctly and remove the mask before testing;

4. Wear it immediately after testing, and prepare a spare mask for easy replacement at any time after pollution.

5.When you feel sour and itchy in your nose or sneeze, you can immediately cover it with a paper towel or elbow.

Before collecting nasopharyngeal swabs and before being tested, the collection personnel shall be informed whether they have relevant past medical history. For example, history of nasal surgery, history of deviation of nasal septum, blood diseases, throat diseases, etc

How long will we know the pcr test results？

The test results of sampling in the morning are generally available from 18:00 to 19:00.

The detection results of afternoon sampling can generally be queried around 22:00.

What’s the difference between the PCR and antigen tests for COVID-19?

Polymerase chain reaction (PCR): PCR is the most reliable and accurate method to detect active infection. PCR tests usually give results in a few hours.

Antigen detection: detect protein fragments on the surface of covid-19. Antigen detection usually takes only 15 to 30 minutes.

How can we prevent COVID-19?

Firstly, keep good personal hygiene habits, wear masks when going out, wash hands frequently, clean the room, and wipe the floor and other furniture with disinfectant. Second, disinfect your hands with 75% alcohol every time you go out and go home, reduce the number of times you go out, and wear qualified masks. And the diet should also be light, do a good job of physical exercise and enhance personal physique.

Most importantly, if our conditions permit, we should vaccinate and conduct multiple nucleic acid tests.

If our Nasal swab pcr test results are positive,what should we do?

If our test results are positive, don’t be afraid. We should wear masks and go to the nearby Hospital for follow-up in time.

Repeat the nucleic acid test.Don’t be afraid and hide the test results. Timely medical treatment is the best choice.

Cell medical (Dongguan) Co., Ltd. is a comprehensive high-tech biotechnology company and an OEM factory of flocking swabs in China.We support custom production,our factory produces flocked swabs, pharyngeal swabs, nasal swabs, oral swabs,Rapid Antigen Test Kit, virus sampling tubes, transportation media, sample preservation solution and nucleic acid extraction kit.

Welcome to leave a message and consult us!

 

 

What is a sterile swab

What is a sterile swab？

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Sterile swab is the full name of disposable sterile sampling swab. The sterile sampling swab does not contain or other live microorganisms. Common sterilization methods include autoclave, EOS (ethylene oxide gas) and γ-ray irradiation. In medicine, we usually use sterile swabs for biological sample collection.  Such as bacterial sample processing, COVID-19, nasal swab sampling, throat swab sampling, oral swab sampling, virological cell culture and so on are ideal ways.

Swabs are sterile to maintain the integrity of the sample. Usually, there is a resealable sterile container or bag to preserve the integrity during processing and transportation to the laboratory. Many different people use sterile cotton swabs, including medical personnel, food manufacturers and crime scene investigators.

Sterile swabs can be used to collect samples. The material, size and shape of the sterile swab head may be different, such as round or pointed. Traditional swab may be made of cotton fiber, polyester or calcium alginate.  Collection and publishing volumes are large and may be corrupted. Traditional swab laboratory staff also need to use scissors to cut, which is too troublesome and time-consuming.Our new flocked disposable sampling cotton swab greatly reduces the time cost and has the advantage of being easier to use than traditional swabs.

The main advantages of our sterile flocked swab are as follows.

1. It has extraordinary water absorption capacity and can increase the number of samples collected on the watch from 20% of the traditional sampling swab to 60%.

2. The release rate of collected samples exceeds 90%, which ensures the high reliability of the results.

3. Select different preservation solutions for different types of samples.

4. The plastic rod has a unique fragile design, which is convenient for sample transportation.

5. Ethylene oxide sterilization, irradiation sterilization and independent packaging.

Sampling operation method.

1.Nasopharyngeal swab sampling.

Nasopharyngeal Sampling diagram

Nasopharyngeal Sampling diagram

①Insert a sterile swab into the nostril of the patient,reaching the surface of the posterior nasopharynx.

②Swab over the surface of the posterior nasopharynx. Hold the swab in the nosefor15-30s and rotate it 3 times.

③Withdraw the sterile swab from the nasal cavity.

④Put virus sampling tube vertically.

⑤At the breaking point, disconnect the swab, discard the tail, and completely place the swab into the tube.

⑥Tighten the tube cap, mark it, put it into the biosafety bag and seal it.

2.Oropharyngeal swab sampling

Oropharyngeal Sampling diagram

Oropharyngeal Sampling diagram

① Keep the head slightly tilted, the mouth open, and the pharyngeal tonsils on both sides exposed.

②The sampling personnel shall wipe the root of the tongue with a swab. Wipe the pharyngeal tonsils on both sides of the subject back and forth with a strong force for at least 3 times, and then wipe the posterior pharyngeal wall up and down for at least 3 times.

③Put the sampled swab into the virus sampling tube, break the swab rod at the mouth of the tube cover and completely put it into the tube.

④Tighten the tube cap, mark it, put it into the biosafety bag and seal it.

⑤Submit for inspection in time.

Anal swab sampling

Anal Sampling diagram

Anal Sampling diagram

①The patient is in the knee chest position or lateral position.

② Gently insert a sterile cotton swab into the anus for 3cm-5cm, and then gently rotate and pull it out.

③Immediately put it into a 15ml outer screw cap sampling tube with 3ml-5ml virus preservation solution.

④Fold off the tail of the cotton swab, tighten the sealing cover, and then submit it for inspection in time.

⑤In order to avoid embarrassment of the examinee, anal swab sampling is generally conducted in a more private place, and the operation requirements of anal swab sampling are more strict. Generally, there will be no obvious discomfort after anal swab sampling.

 

Nasopharyngeal swab collection kits with virus transport media

Nasopharyngeal swab collection kits with virus transport media

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Nasopharyngeal swab collection kits with virus transport media

Cell medical (Dongguan) Co., Ltd.

Nasopharyngeal swab collection kits are used to collect nasopharyngeal swab samples,which is a high quality and reasonable kit for testing COVID-19 .

The collection kit contains virus transport media, which can collect, transport and store viruses at room temperature The ability of these kits to detect samples by RT-PCR has been verified. The virus and related samples shall be stored and transported within 48 hours under cold storage (2 ~ 8 ℃).

Characteristics of nasopharyngeal swabs and virus transport tubes.

The sterile nasopharyngeal swab we provide has a flocked tip swab with a breakpoint located about 80-90 mm from the tip, which is compatible with the catheter provided. The virus sampling tube cap design is very effective in containing media and preventing sample leakage.And these sampling tubes are pre labeled, and each tube has a unique bar code identifier.

Nasopharyngeal swab collection kits with virus transport media

Nasopharyngeal swab collection kits with virus transport media

Product specification

A) 1pcs flocked swab ,1 pc 10ml tube with 3ml medium refilled；
B) 2pcs flocked swab ,1 pc 10ml tube with 3ml medium refilled；
C) 1pcs flocked swab ,1 pc 5ml tube with 2ml medium refilled；
D) 2pcs flocked swab ,1 pc 5ml tube with 2ml medium refilled；

certification

• CE IVD registration Kit；

• iso13484 certified manufacturer, registered with FDA；

 

Related products

Medical Nasal Swabs Kits Std Swab Virus Transport Medium

Nasopharyngeal Swabs Virus Sampling Transport Vtm Tube

Flocked Swab+Tube With Viral Transport Medium

Foam Swab VTM Kit

Contact us

Cell medical (Dongguan) Co,Ltd.

  • skype：+8618814476009
  • E-mail：flockedswabs@gmail.com
  • No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China
New Coronavirus nucleic acid detection

New Coronavirus nucleic acid detection steps

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New Coronavirus nucleic acid detection steps

1、 The specific operations are as follows

1. Virus sampling:

Take the patient’s saliva sample or nasopharyngeal flocked swab sample, and save the virus sampling tube.Virus preservation solutions commonly used in disposable virus tubes include inactivated and non inactivated types. The following are two models of virus transport medium:

1) Inactivated type: the inactivated virus transport medium characterized by killing the virus and retaining nucleic acid fragments. Application:detect COVID-19, influenza virus, hfmv and influenza A.

2) Non inactivated type: the non inactivated virus transport medium does not contain lysate. It can retain the activity and integrity of pathogens.Application:virus culture and reproduction to make the detection results more accurate.

flocked swab specimen collection_New Coronavirus nucleic acid detection steps

flocked swab specimen collection_New Coronavirus nucleic acid detection steps

2. Nucleic acid extraction:

Extract the genetic material from the patient’s sleeping fluid or nasopharyngeal swab sample. If a patient carries a virus, there will be RNA of the virus’s genetic material in the sample. Sampling personnel should pay attention to prevent RNA degradation when extracting RNA. At the same time, the extracted DNA shall be stored at – 20 ℃, and the RNA and DNA requiring long-term storage shall be stored at – 70 ℃ or liquid nitrogen.

3. Reverse transcription synthesis of cDNA:

reverse transcription of RNA in the extract, reverse transcription of RNA into cDNA, reverse transcription of cDNA synthesis reaction, using reverse transcription primers, dNTPs, reverse transcriptase, RNase inhibitor and DTT. Buffer, an appropriate amount of ultrapure water without RNA / DNase and RNA template. We propose to use commercially available RT-PCR one-step reagent for one round amplification of di.

4. PCR amplification reaction (nested PCR amplification method with secondary amplification):

Use specific primers of virus cDNA for PCR amplification in an amplification instrument. Nested PCR amplification using secondary amplification.

5. Result analysis and judgment:

If the DNA band of the virus is amplified, it is determined that the virus exists in the patient; If no DNA band is amplified, it is determined that the sample taken by the disease has no virus.

2、 Experimental precautions:

① We should set up two positive controls and two negative controls for each test. Only when the positive control enlarges the expected fragment, the negative control does not enlarge any fragment, and the results of two parallel samples are consistent.We can determine the nucleic acid positive or negative reaction results.

② Nucleic acid test is positive: if nucleic acid is positive, we should repeatedly collect samples for retest. If the retest result is nucleic acid positive, we can confirm that the sample result is nucleic acid positive. If the retest result is nucleic acid negative, the nucleic acid test result is uncertain.

Facing COVID-19 variation strain

Facing COVID-19 variation strain

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Facing COVID-19 variation strain. How should we deal with it？

What are the variant strains of COVID-19?

The variation of COVID-19 B.1.621 was first confirmed in Columbia in January this year. The World Health Organization named it Miao strain and recently listed it as a mutant strain that needs attention. Its influence level is lower than that of the delta strain. Preliminary data show that the Miao strain is similar to the beta strain and will weaken the effectiveness of the antibody.

At present, there are four variants defined by who as “needing attention”, including alpha, beta, gamma and delta. And five variants “needing attention”, including ETA, iota, kappa, lambda and mu.

How should we deal with it？

Weightier than Mount Tai, I think the most important thing about this new crown epidemic is: life is above everything else and responsibility is heavier than Mount Tai.

We often ask: where is the value of life? After this epidemic, we answer is: responsibility.

A person and an enterprise must have a sense of responsibility before their life can be valuable. If a person is not responsible and has no sense of responsibility, his life is worthless.

First, Let’s do a good job in personal protection. Don’t worry too much.

As the general public, we can prepare some masks, medical goggles,Rapid Antigen Test Kit,New Coronavirus test kits and so on.We advocate new crown nucleic acid testing at home

Rapid Antigen Test Kit specimen testing

Rapid Antigen Test Kit specimen testing

Cell medical (Dongguan) Co., Ltd. is a comprehensive high-tech biotechnology company and an OEM factory of flocking swabs in China.We support custom production,our factory produces flocked swabs, pharyngeal swabs, nasal swabs, oral swabs,Rapid Antigen Test Kit, virus sampling tubes, transportation media, sample preservation solution and nucleic acid extraction kit. Our GMP factory was built in 2016,the factory has ISO13485 quality management system, CE, FDA and other qualifications.

Secondly, we should keep regular work and rest and keep learning.

Adhere to exercise and treat your body: regular work and rest, balanced diet, exercise and regular physical examination. After all, the body is the capital of revolution, and immunity is the first competitiveness.

Finally, if your physical condition permits, please vaccinate as soon as possible. For you, for your family and for our national peace and security.

In order to better prevent COVID-19 variation strain, Cell medical (Dongguan) Co.,Ltd. increases production and guarantees supply.