What is Viral Transport Media

Posted on 2022年3月14日2022年3月30日 by Dr. Lee

What is VTM?

VTM is the acronym for Viral Transport Media, which contains nutrients that carry the virus and keep it active so that the sample can reach the laboratory for testing.

VTM virus preservation solution is suitable for the collection, preservation and transportation of common virus samples.

Such as new coronavirus, influenza virus, hand, foot and mouth virus. It can collect throat swabs, nasal swabs or tissue samples from specific parts.

And the stored samples can be used for subsequent nucleic acid extraction or Purification and other clinical experiments.

Virus Transport Medium (VTM) Definition

The virus transport medium sampling tube is a complete set of centrifuge tubes for sampling and transporting viruses.

What is Viral Transport Media

Such as new coronavirus, influenza virus, hand, foot and mouth virus in the microbial sampling transport tube.

Also known as the specimen transport tube. commoditized products.

A nutrient is used to carry and maintain the viability of test specimens (viruses) for the identification and further processing of samples in laboratories.

Viral Transmission Media Principles (VTM)

Since there is no cellular structure. Viruses cannot replicate themselves, but instead, invade genes into host cells and replicate new viruses with the help of the latter’s replication system.

After the virus sample is collected, in order to maintain the activity of the virus sample and prolong the survival time of the virus in the sample, the sampling swab will be put into the transportation medium for storage and transportation.

Transportation medium composition and inspection principle:

1. The combination of various antibiotics has broad-spectrum antibacterial and antifungal effects.

2. As a protein stabilizer, bovine serum albumin (BSA) can form a protective film on the protein shell of the virus, making it difficult to decompose and ensuring the integrity of the virus

3. The neutral environment constructed by Hank’s buffer helps to increase the survival time and infection stability of the virus.

4. Phenol red is an acid-base indicator, the discoloration area is 6.6 (yellow)-8.0 (red), and it is red at 7.2-7.4.

Vtm Kit

The most stable pH value of the viral outer layer protein is 7.2-7.4, which is also the pH value range of Hank’s buffer. If contaminating molds or bacteria are not inhibited by antibiotics, they can multiply, releasing carbon dioxide and lowering the pH.

At this time, the appearance of the sampling solution will change from red to yellow.

Notes:

To produce an isotonic solution containing proteins to protect viral structures, antibiotics to control microbial contamination, and one or more buffers to control pH.
Use the concept of assays such as nucleic acid amplification assays (NaAt) to prepare viral delivery media while preventing samples such as drying.

Composition of viral transport media (VTM Kits)

There are mainly the following parts:

1. Sampling swab with disposable sterile plastic rod/rayon head;

2. Sterile sampling tube containing 3ml of virus maintenance solution;

In addition, there are additional parts such as a tongue depressor, a biosafety bag, etc.

Viral delivery media typically have protective protein components, antimicrobial agents to prevent the potential for contamination, and buffers to maintain the pH of the solution.

Some VTMs also have additional ions and minerals that help keep such samples viable.

How to use Virus Transfer Media?

Liquid transfer media are primarily used to transfer the swab or material from the collection swab into the media.

It facilitates the collection and transport of samples in areas where it is not available.

Precautions for use:

1. Before sampling, indicate the sample information on the label of the cryopreservation tube;

2. According to different experimental purposes, use sampling swabs to sample at the corresponding parts (the most commonly used are nasopharyngeal swabs, pharyngeal swabs are the oral swabs in the product);

3. Immediately after sampling, put the swab into the sampling tube containing the virus transport medium, break it at the breaking point, and tighten the tube cover;

4. The specific sampling methods are as follows:

a) Nasal swab: Gently insert the flocked swab head into the nasal palate, stay for a while and then slowly turn to exit. Swab the other nostril with another swab, immerse the swab head in the sampling solution, break it at the breaking point, and discard it.

b) Pharyngeal swab: Wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with a flocked swab, and also immerse the swab head in the sampling solution and discard it.

Flocked Cotton Throat Oral swab 15Cm

Nasal Swab Covid Test

Posted on 2022年3月10日2022年3月22日 by Dr. Lee

Nasal Swab Covid Test

Some people need to be tested for COVID-19 with a quick swab.

The pros are sure: If it is possible for you to test both the nose and the throat at the same time.

Officially, the FDA follows inspection guidelines. Simply put, observe the subtests consistently during this period.

COVID19 test flocked swabs to exteriors.

What is a nasal swab?

COVID Swab test

A nasal swab is a test that checks for viruses in the nasal cavity, and the result is a test that checks the nasal cavity by a method.

Because of rhinitis in the nasal cavity, sinusitis, sinusitis, nasal cavity in the nasal cavity and other problems.

There are several respiratory infections, including the flu, COVID-19. A nasal swab can help your doctor determine the type of infection you have and which treatment is definitely best for you.

The test can be done by taking a sample of cells from your nostrils or nasopharynx.

A nasopharyngeal flocked swab is the uppermost part of the nose and throat.

Other names: Anterior nostril examination, turbinate swab, NMT swab, nasopharyngeal culture, nasopharyngeal swab

Why do I need a nasal swab?

01The positive detection rate of nasal swabs is higher.

When using a throat swab to detect the new coronavirus, the base of the tongue will instinctively prevent the cotton swab from going deep, and the cotton swab is not easy to reach the back wall of the pharynx. . The nasal swab is easier to reach the tested site and can stay in the nasopharynx for a long time, and the test results are more accurate. Students returning to school from the north and south of the river and by various means of transportation require us to improve the accuracy of testing in a responsible attitude to everyone’s health.

02Nasal swabs are safer for nucleic acid collection personnel.

During the nasal swab test, the tested person needs to wear a mask with only the nostrils exposed; while for the throat swab test, the tested person needs to open his mouth wide, and the exposed area is large. If the tested person coughs and retches, the nucleic acid tester may face a greater risk of infection. Once the testers are infected, the risk may spread to a wider range of people.

03 Throat swabs may be more uncomfortable than nasal swabs.

Throat swabs are collected across the base of the tongue to the posterior pharyngeal wall, tonsillar recesses, sidewalls, etc., and repeatedly wiped 3 to 5 times to collect mucosal cells. In response, nasal swabs were relatively less irritating to the mucosa.

The entire nasopharyngeal swab process:

nasopharyngeal swab

You will throw your head back.
Your healthcare provider will insert the swab directly into your nostril until it reaches your nasopharynx (the top of your throat).
Your supplier will rotate the swab and remove it.
Do I need to do any preparation for the exam?
For nasal swabs, you don’t need any kind of special preparation.

Nasopharyngeal Swab Collection Instructions

Posted on 2022年2月24日2022年3月22日 by Dr. Lee

Nasopharyngeal swab sampling： A thin flocked swab into the nostril, from the lower nasal meatus to the posterior wall of the nasopharynx.

And then twisting the swab for sampling.

The depth of the sampling swab into the nasal cavity is approximately the distance from the tip of the nose to the earlobe.

How to collect a Nasopharyngeal (NP) swab

1. Keep your head still, to remove the secretion on the surface of the anterior nostril;

2. Measure the distance from the tip of the nose to the earlobe with a swab as well as mark it with your finger. Put the swab right into the nasal dental caries in the direction perpendicular to the nose (face), and also reach the posterior nasopharynx when it comes across resistance, to make sure that the swab stays in the nose for 10-15 s, gently rotate 3 to 5 times to secure the examination piece.

There is definitely no demand for the sampler to stand straight opposite the patient. The advantage of standing on the side is that if the patient has coughing, sneeze, etc, it can be avoided in time.

It is vertical to the coronal airplane of the head or the face and also permeates deeply from the inferior meatus to the posterior wall of the nasopharynx, as well as it is enough to feel the wall surface. The depth of the nasopharyngeal swab right into the nasal tooth cavity is about the range from the pointer of the nose to the earlobe (see picture).

Nasopharyngeal sampling

3. The nasopharyngeal swab must be turned on the nasopharyngeal mucosa, maintained for 10-15 secs, and after that got rid of. The kid’s participation is poor. In practice, we typically obtain the swab in three to five secs after completely twisting it 2-3 times.

Nasopharyngeal swab vs nasal swab

A nasal swab (often called an anterior nose test): A sample taken by touching both sides of the nasal wall. The nasopharyngeal swab is to probe from the nose to the throat.

What is the nasal swab technique?

The nasal swab approach uses a swab to accumulate an analysis example from the nasal membrane. The nasal swab examination (frequently described as the former nasal test) involves putting the swab around 0.5-0.75 inches deep into the nostril as well as turning it in one nostril for 10-15 seconds while touching both sides of the nasal wall, then making use of the same cotton swab, Gather in the other nostril similarly. Since this approach is less invasive, it is usually extra comfortable for the patient – and is additionally the approach utilized when self-testing.

Nose structure

Nasopharyngeal swab

Impacted by elements such as age and also face structure development, there are private differences in the collection deepness of nasopharyngeal swabs.

We should pay attention to personal protection when collecting nasopharyngeal swabs, and clean the nasal cavity before collection.

If the patient has nasal septum curvature, the background of nasal surgery, rare coagulation characteristics, etc., the patient should inform the collection staff in advance to avoid damage to nasal caries.

During collection, the patient should tilt his head back to ensure smooth entry of the nasopharyngeal swab.

It might be accompanied by discomfort and discomfort in the nasal tooth cavity.

Furthermore, nasal bleeding might happen after nasopharyngeal swab collection.

A percentage of blood loss does not call for unique therapy. If the amount of blood loss is huge or lasts for a long time, you must go to the hospital for hemostasis therapy in time.

Additionally, clients with signs and symptoms such as edema as well as blood loss in the nasopharynx should stay clear of nasopharyngeal swab collection to avoid worsening the problem. Before the nasopharyngeal swab collection, you must avoid taking antibacterial and also antiviral drugs to stay clear of impacting the examination results.

Cell medical nasal swab adopts jet nylon fiber implantation technology, which is first-class in the domestic and foreign markets. Such material can absorb more samples.

Cell medical Flocked swab

How to Use Specimen Collection Kit

Posted on 2022年2月22日2022年3月22日 by Dr. Lee

What is a Specimen Collection Kit?

Sample collection kits provide all the components needed to secure samples and send them to laboratories for diagnostic. Environmental sampling and research purposes. Timely collection and processing of samples are critical to ensure accurate results and prevent analytical errors.

Specimen Collection Kit Contains:

Individually packaged sterile specimen collection flocked swab;
10ml virus sampling tube, containing 3ml Viral Transport Media;
Sterilization packaging, Individual in Paper peeled pouch;

The novel coronavirus (2019-nCoV) is an RNA virus. Improper storage or transportation of samples after collection may result in the degradation of part of the nucleic acid and affect the test results;

In addition, samples that have not been inactivated virus can easily lead to indirect infection of operators.

The covid-19 sampling kit independently developed by Cell medical can not only prevent the degradation of viral RNA but also inactivate the virus.

While ensuring the test results, it can reduce the risk of biological infection during the inspection process.

Product description

Viral Transport Medium Chin, VTM kits For COVID-19. Cell medical not only produces sampling swabs but also virus sampling tubes.

Analyzing material effectively starts with collecting samples with the right tools, whether it’s collecting samples from the body to aid in medical diagnosis, identifying potentially harmful pathogens from the environment.

Or collecting samples from crime scenes to aid in investigations.

However, it is also important to ensure that contaminants do not come into contact with the sample as it travels to the laboratory. Given the plethora of options available for each of these missions, choosing the right collection and transport vehicle can be overwhelming. This is where a specimen collection kit can prove invaluable.

Features:

1. Safety: Sampling and virus inactivation are carried out simultaneously, which greatly reduces the operator’s infection risk;

2. Stability: keep the sample stable, avoid nucleic acid degradation, and ensure the accuracy of the test results;

3. High efficiency: Inactivated virus samples can be detected in conventional PCR laboratories, and the collection tube can be adapted to the Tianlong automated nucleic acid extraction system, which improves the detection efficiency of samples.

Instructions:

What is the Specimen Collection Kit?

As pointed out over, example collection packages have shown very usefully in securing examples for analysis in a large range of clinical, environmental and research study applications.

Nasopharyngeal samples collected by swabbing the upper retronasal vocal cords can supply analysis confirmation for a series of viral as well as bacterial infections, consisting of influenza, COVID-19, enterovirus D68, as well as other respiratory system conditions.
Fecal samples can be gathered by anal or fecal swabs as well as can aid clinicians detect a series of intestinal microbial infections.
A vaginal swab example can help determine the visibility of Group B Streptococcus or Streptococcus agalactiae, a type of microorganisms found in the digestive system and also birth canals of a quarter of pregnant ladies, to name a few conditions.
Food sampling is not just important for recognizing the potential presence of foodborne pathogens but is a crucial step in maintaining conformity with FDA food security requirements.

How to choose the right specimen collection kit?

It is necessary to discover a sampling collection set created for your particular end-use. This consists of using the flocked swab that best matches your sampling collection needs. As an example, foam swabs might use elution characteristics best matched for environmental and also food sampling, while polyester or crowded swabs may supply a combination of collection attributes and also person comfort far better fit for medical usage.

Nonetheless, it is not constantly necessary to utilize a sample collection package created for usage with the example cpu’s very own brand name of the fluid transfer system. Validation examinations can aid clinicians, food mill and also other samplers, and also labs recognize precise and also affordable sample collection kits. Verifying shipping systems from several suppliers has come to be a common practice in research laboratories around the globe, not just to ensure sustainable supply, but likewise to compare item top quality and expense.

Viral Transport Medium Tube

Posted on 2022年2月18日2022年2月18日 by Dr. Lee

Viral Transport Medium Tube

A virus sampling tube is a product used to collect and transport infectious disease specimens. It is not a single product, but a set consisting of a sampling flocked swab, Viral Transport Medium and a plastic tube. Sampling process: use a swab to sample the patient, but the preservation solution into a plastic tube, put the sample into the plastic tube preservation solution, and transport the preserved sample to the laboratory for testing.

Special instructions for virus sampling tubes:

If the collected sample is used to detect viral nucleic acid, it must be used with a nucleic acid extraction kit and nucleic acid detection reagent; if it is used for virus isolation, it must be combined with a cell culture medium.

Different application fields have different requirements on the volume of sampling liquid, please replace the corresponding product according to the prompts in the sequence information:

Viral sampling tubes that collect viral samples from clinical patients generally require less fluid.

For a collection of single-use virus sampling tubes and short-term delivery of influenza virus from the external environment. We typically require a liquid volume of 3 to 6 ml.

Virus sampling tubes have three requirements, including transport, storage, and sample usefulness.

Transportation, ensure that the samples do not leak during transportation;

Preservation, ensure that the sample does not leak during the preservation process;

In terms of the usefulness of the sample, the sampling tube itself does not have a toxic effect on the sample, so as not to affect the material collected in the sampling tube, which in turn affects the test results.

What is vtm kits?

Viral Transport Medium Tube

Cell medical (Dongguan) Co., Ltd.’s disposable nasopharyngeal VTM sampling kit is a nasopharyngeal swab sample collection for COVID-19 detection. The kit contains a virus transport medium (VTM) for nucleic acid detection and virus culture. It can be stored at room temperature before collection and should be stored at 2-8°C after collection to avoid the growth of contaminants.

VTM

Viral Transport Medium includes Hanks’ Balanced Salt Solution, fetal bovine serum, antibacterial antibiotics, antifungal antibiotics. And phenol red as indicators for real-time monitoring of solution pH stability.

It is not a single product, but a set consisting of a sampling flocked swab, Viral Transport Medium and a plastic tube.

Flocked swab

The supplied sterile nasopharyngeal swab has a flocked tip with a breakpoint at 80mm of the tip, compatible with the supplied tubing. The cap design is very effective in containing the VTM and preventing sample leakage.

Viral Transport Medium kits Specifications

Specifications	1 nasal flocking sampling swab + 1 bottle of 10ml preservation solution, containing 3ml liquid
Liquid Volume:	1mL/2mL/3mL/5mL

Click here to learn more.

Flu Swab Test

Posted on 2022年2月8日2022年3月22日 by Dr. Lee

Influenza, commonly known as influenza, is an acute respiratory disease caused by influenza A, B, and C viruses, and belongs to Class C infectious diseases. Influenza is an illness that spreads relatively easily from person to person and can infect large numbers of people. While most people recover quickly from the flu, some, such as older adults, are at risk of serious illness and even death.

Flu Swab Test

Influenza viruses come in many different forms. A flu swab is a method of taking a sample from a person. Medical staff check patients for flu. If so, what kind of flu virus can be spread by inhalation through the nose and throat of an infected person.
When the flu virus infects a person, it spreads through the respiratory tract. The nose and throat are where the virus lives and grows, causing symptoms such as a runny nose, sore throat and cough. These places are also the easiest places for doctors to obtain samples. As a result, doctors can often use flu swabs to determine whether a patient has the flu, which often spreads quickly in a population.
Due to public health, some government medical agencies may wish to track the spread of the disease. Since influenza viruses also mutate rapidly, new influenza viruses are produced regularly. The type of flu a person has can also be important information for the public health system.
Finally, a doctor may need to determine whether a person has the flu so an appropriate treatment plan can be instituted. There are several ways a doctor can use a swab to determine if a patient has the flu virus. An area of medical professionals, usually to test for microbes. (Nasal swab for flu)

A sampler is doing a swab test for a woman

How to Swab for the Flu?

They usually have a long swab stick with a soft swab tip at the end, made of cotton material, polyester, polyester or rayon tip, nylon flocked swabs. is sterile, so the test only analyzes the microbes in the sampled area. During the flu swab procedure, doctors usually insert the end of the swab into the patient’s nose and spin the flu swab to collect as much mucus as possible.
A doctor can take a sample by inserting a swab into the top of the throat through the nose. If the virus grows heavily in the area, the flu can cause dizziness.
Doctors have several different ways to test a cotton swab for the flu virus. The test that takes a few days to a week to complete is a viral culture, in which viral particles are grown in human cells.
Rapid tests include tests that analyze the sample for the presence of genetic material or antigens from the flu virus. An antigen is a special molecule in a virus. Some commercial tests contain antibodies, and the presence of antigens can be recognized by their linkage to the antigens.
These tests are less than an hour, but may not be as accurate as viral cultures. A runny nose and sore throat can be symptoms of the flu.

How do I know if I have the flu?

If you have a fever, cough, sore throat, runny or stuffy nose, body aches, headache, cold and/or fatigue, your respiratory illness may be the flu (flu). Some people may experience vomiting and diarrhea, although this is more common in children. People can get the flu without having a fever. Influenza viruses usually cause the most illness during the colder months of the year. However, the flu can also occur outside of a typical flu season. In addition, other viruses can cause flu-like respiratory illnesses. Therefore, it is not possible to tell if you have the flu based on symptoms alone. If your doctor needs to determine if you have the flu, you can do laboratory tests.

Can I get the flu and COVID-19 at the same time?

Yes. Co-infection with influenza and other respiratory illnesses, including COVID-19, is possible. Health experts are still studying how common the condition is.

The flu is similar to some of the symptoms of COVID-19, so it’s hard to tell them apart based on symptoms alone. The swab diagnostic test helps determine if you have the flu, COVID-19 or other respiratory infections.

Virus Transport Medium Composition

Posted on 2022年1月29日2022年1月29日 by Dr. Lee

VTM Transport Medium

Transportation Medium Specifications

2.0ml, 3.0ml, 4.0ml, 5.0ml, 6.0ml.

Application

For transport storage of clinically collected samples.

Virus Transport Medium kits

Virus Transport Medium Composition

Hank’s balanced salt builds a neutral environment; bovine serum albumin as a protein stabilizer and Hank’s balanced salt increase the survival time and infection stability of the virus; antibiotics have broad-spectrum antibacterial effects; acid-base indicator, pH 6.6 in the discoloured area (yellow) to 8.0 (red), red at pH 7.2 to 7.4.

Storage condition: 4-25℃ Validity period: 12 months.

How to use VTM Kits?

1. According to the sampling requirements, use a sampling swab to collect samples.

2. Place the sampling swab after sample collection into a sterile sampling tube. 3. Break the swab at the breaking point of the sampling swab rod and discard the tail. 4. Tighten the cap of the sterile sampling tube.

5. Label the sterile sampling tube with information as required.

6. Specimens should be transported to the corresponding influenza surveillance network laboratory within 2 working days after collection, and the storage temperature should be 2-8°C; Save and ensure that the collected specimens are sent to the corresponding network laboratory within 1 week. Specimens should avoid repeated freezing and thawing.

how to use flocked swabs

7. For specific sampling methods, please refer to the following:

1) Throat swab specimen: Use a special sampling swab to wipe the posterior wall of the pharynx and the tonsils on both sides with moderate force, avoiding touching the tongue; quickly immerse the swab head in the sampling solution, and it is easy to break at the sampling swab rod. The swab is broken at the cut-off point and the tail is discarded. (Applicable to this product sampling)

2) Nasal swab specimen: use a special sampling swab, gently insert the swab head into the nasal palate, stay for a while and then slowly turn it to exit; quickly immerse the swab head in the sampling solution, and the swab head is easy to remove in the sampling swab rod. Break the swab at the cutoff point and discard the tail. (Applicable to this product sampling)

Why Choose Anal Swab Covid Tests

Posted on 2022年1月22日2022年1月22日 by Dr. Lee

Why Choose Anal Swab Covid Tests

A friendly netizen said,

“The anal test first asks you to take off your pants and lie on the bed,

The paramedic then stabs (anus) twice with an anal swab,

shallow once deep

In addition to the endless shyness in the whole process,

Nothing else feels like it! ”

A shy handsome man

Xiaolee will tell you why you need to do anal swabs.

Currently screening for COVID-19,

Mainly use an oropharyngeal swab and nasopharyngeal swab nucleic acid detection,

Because of its convenience and speed, it is suitable for large-scale screening;

Nasopharyngeal Swab Sample

However, some asymptomatic or mildly infected people,

The disease recovered quickly after infection,

Nucleic acids in the respiratory tract and pharynx are undetectable within 3-5 days.

longer duration of nucleic acid-positive stool or anal swab,

Therefore, adding anal swabs can improve the detection rate,

Effectively reduce the probability of false negatives,

Reduce missed diagnoses!

One of the anal swab samplings poses

Friends who have done nucleic acid swab tests have experience,

In previous tests,

The most uncomfortable part should be the part of the nasopharyngeal swab,

To poke the nose is to poke deep into the soul.

According to the description of the anal swab recipient,

“The whole process was lying in bed and the medical staff helped.

Insert the tip of the sampling cotton swab into the anus and turn it a few times.

Twice in total, each for about 10 seconds.

Although a little surprised and a little embarrassed,

But understandable and bearable,

After all, this rule is for everyone’s sake. ”

Many Chinese friends who have returned to China for quarantine

I have also experienced the subtle taste of it.

What is Pooled Sample Test?

Posted on 2022年1月20日2022年1月20日 by Dr. Lee

Pooled Sample Test?

With the 2019 coronavirus disease (COVID-19) pandemic, doing a good job in viral nucleic acid detection is the top priority. However, detection of the virus is still limited due to resource constraints. Pooled sample testing offers a way to significantly improve testing power.

“mixed nucleic acid test” or “Pooled Sample Test”, referred to as “mixed test”.

Which means that two or more samples are mixed and tested. method for positive samples.

What is the difference between the “mixed collection” of community nucleic acid testing and the “single collection” of the hospital?

Why do community streets adopt the mixed mining model?

Under what circumstances should I test individually?

What is pooled COVID testing?

The so-called “mixed detection” of nucleic acid.

Takes throat swab samples from multiple people and puts them together for viral nucleic acid detection.

Pooled Sample Test VTM Kits

In fact, Medical use of Pooled Samples is common.

In blood collection systems such as apheresis stations.

We use pooled samples to detect the presence of infectious disease antigens and antibodies in pooled samples of multiple people.

There are two modes of “mixed detection”. One is to sample several people, such as 3 or 5 people and put them into the same sampling tube.

The other is to mix samples of the same volume from 3 or 5 people during laboratory testing, also known as “sample mixing”.

From a scientific point of view, the first “mixed sampling” mode will not affect the sensitivity of nucleic acid detection. While the latter mode of mixed detection of samples has a certain impact on the sensitivity of the detection, the degree of impact is controllable.

In order to detect high-risk groups, such as symptomatic patients and close contacts in fever clinics, we still recommend separate sampling.

For screening of low-risk groups, pooled sample test is our first choice.

Is the mixed detection technology reliable?

Is the “five-in-one” and “ten-in-one” mixed mining detection technology reliable?

“The size of the single-collection tube and the mixed-collection tube are different, and the amount of the preservation solution is also different.

Based on the results of a large number of basic experimental research and practical operations in the previous period.

The increase in the volume of the mixed-collection preservation solution has no effect on the detection results of weakly positive specimens.”

In the case of an extremely low positive rate, the 10-in-1 mixed detection technology is very efficient.

The FDA is aware that there is a great interest in performing testing using pooled samples.

This approach increases the number of individuals who can be tested with the same amount of resources.

What are the benefits of mixed nucleic acid detection?

The hybrid detection of nucleic acid has greatly improved the nucleic acid detection capability, reduced the burden of personal testing costs, and did not affect the time for issuing the test report.

The testing time of a single test and mixed test laboratory is the same, but compared with a single test, the mixed test is more suitable for mass and group screening.

In the case of small-scale outbreaks, the use of mixed detection can improve efficiency, play the role of multiple screening, early diagnosis, reduce transmission, and save social costs.

Whether the population is suitable for mixed testing shall be carried out in accordance with the requirements of the provincial and municipal epidemic prevention and control headquarters,

The medical institutions carrying out the testing must follow the technical requirements and standards of the municipal health department.

Which groups recommend the single purchase and single inspection?

It is worth mentioning that single sampling and single inspection are recommended for specific populations.

The above notice specifically mentioned that COVID-19 nucleic acid 10 in 1 and 5 in one mixed detection technology is suitable for large-scale rapid screening of the low-risk populations, and is more suitable when the overall positive rate of population is low (0.1%).

However, for the detection of high-risk groups such as symptomatic patients, close contacts and people in key areas in fever clinics.

Recommended to adopt single sampling and single detection.

What should I pay attention to when doing pooled sample detection?

1. When going to the testing point for nucleic acid testing, wear a mask, and it is best to prepare one or two spare masks.

2. Two hours before the swab test, it is recommended not to eat to avoid vomiting during the test.

3. When waiting for the test, remember to keep a distance of more than one meter between the front and rear personnel to avoid close contact.

4. Whether it is doing swab testing or getting the report, it needs to be certified as one, so be sure to bring ID cards, receipts and other materials, so as to avoid incomplete materials at that time, which will affect the testing and reporting.

How to Swab For Covid Test

Posted on 2022年1月15日2022年3月22日 by Dr. Lee

How to Swab For Covid Test

Since the outbreak of novel coronavirus pneumonia, we must have experienced nucleic acid testing. Covid swab test Nucleic acid detection plays a very important role in the process of infection diagnosis of COVID-19. It can not only find asymptomatic patients in the incubation period as much as possible but also greatly reduce the risk of infection.

Nucleic acid detection is so important. Next, Dr Li will take you to have an in-depth understanding of the relevant principles of nucleic acid detection, the difference between mixed collection and single collection of nucleic acid detection, and the matters needing attention in nucleic acid detection, to help you better protect yourself while popularizing knowledge.

To understand nucleic acid detection, we must understand the detection principle. Let’s take a look at its detection principle first.

The doctor is testing the patient’s nasopharynx swab

What is the principle of nucleic acid detection?

Nucleic acid, the genetic factor of the virus, is a macromolecular chain composed of four nucleotides. Different molecules represent different nucleotides and form a long chain. Nucleic acid detection is through the detection of nucleic acid in the chain, and this segment of nucleic acid is COVID-19 specific, other viruses do not.

Pcr test nasal swab and an antigen test difference for covid 19

The substance detected by the nucleic acid is the nucleic acid of the virus. At present, the detection technology is mainly through PCR (polymerase chain reaction) technology to detect the nucleic acid of the virus. During the detection, the nucleic acid chain of COVID-19 was used as the detection target, and the target gene sequence was amplified by PCR Test, which was combined with a fluorescent labelled probe added in the test reagent to produce a fluorescence signal.

The three forms of nucleic acid detection have their advantages.

Nasal swab test

This test method is to insert the test swab vertically into the nostril. And gently rotate the swab, stay in the nasal cavity for about 15 seconds, to test.

Flocked Tip Swab Nylon Sterile Nasal Swab Stick

Compared with throat swabs, nasal swab testing is more accurate for inexperienced testing personnel. And the risk of exposure is lower. However, it is not suitable for some patients with nasal allergies, high blood pressure and people who take anticoagulation and antiplatelet for a long time.

Throat swab test

At present, the most widely used detection method is also a form that is often used by everyone. During the sampling process, the patient opened his mouth and made an “ah” sound, and the testing personnel used a sterilized cotton swab to gently and quickly wipe the pharyngeal lateral wall and posterior pharyngeal wall of the subject many times.

COVID-19 Testing:PCR swab test,antibody test, and antigen test difference

COVID-19 Testing: Throat swab test

Throat swab testing: Often used in the testing process because it is more convenient and fast to collect, and it is more acceptable to the public, but the risk of exposure is also greater.

Anal swab test

This test method is a method of inserting a test swab into the anus and taking a sample at the fold of the anus or taking a sample at 2 to 3 cm for a new coronavirus test.

Many patients reject anal swab testing, but anal swabs can improve the accuracy of testing for new coronavirus infections to a certain extent. Some patients indeed have negative throat swabs, but positive ones are detected by anal swabs. In some patients, the anal swab test was still positive after the other two tests turned negative.

Anal swab

Why do multiple nucleic acid tests need to be performed?

Multiple nucleic acid tests are necessary for epidemic prevention and control.
A negative nucleic acid test does not mean that the tested person is not at risk of infection. Any detection method has the possibility of errors, especially in high-risk areas. To ensure the accuracy of the inspection results and better prevent and control the spread of the epidemic, multiple rounds of inspections must be carried out. There are also the following reasons for taking multiple rounds of detection:

After virus infection, the human body has a certain value-added period. When testing, if the virus in the body does not reach a certain concentration, we may not detect the virus.

When the specimen was collected, due to the different sampling methods, no virus cells were collected. False-negative specimens can also lead to inaccurate collection results.

Errors in the transportation process and storage of samples for inspection will also affect the results of nucleic acid testing.

Therefore, for risk areas and special populations, multiple rounds of nucleic acid testing must be carried out to more accurately and effectively detect the virus source, so as to better and more accurately prevent and control and identify infected people, and cut off the source of the virus. No matter what kind of inspection process, attention must be paid.