Tag Archives: DNA samples

Nasopharyngeal swab collection kits with virus transport media

Nasopharyngeal swab collection kits with virus transport media

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Nasopharyngeal swab collection kits with virus transport media

Cell medical (Dongguan) Co., Ltd.

Nasopharyngeal swab collection kits are used to collect nasopharyngeal swab samples,which is a high quality and reasonable kit for testing COVID-19 .

The collection kit contains virus transport media, which can collect, transport and store viruses at room temperature The ability of these kits to detect samples by RT-PCR has been verified. The virus and related samples shall be stored and transported within 48 hours under cold storage (2 ~ 8 ℃).

Characteristics of nasopharyngeal swabs and virus transport tubes.

The sterile nasopharyngeal swab we provide has a flocked tip swab with a breakpoint located about 80-90 mm from the tip, which is compatible with the catheter provided. The virus sampling tube cap design is very effective in containing media and preventing sample leakage.And these sampling tubes are pre labeled, and each tube has a unique bar code identifier.

Nasopharyngeal swab collection kits with virus transport media

Nasopharyngeal swab collection kits with virus transport media

Product specification

A) 1pcs flocked swab ,1 pc 10ml tube with 3ml medium refilled；
B) 2pcs flocked swab ,1 pc 10ml tube with 3ml medium refilled；
C) 1pcs flocked swab ,1 pc 5ml tube with 2ml medium refilled；
D) 2pcs flocked swab ,1 pc 5ml tube with 2ml medium refilled；

certification

• CE IVD registration Kit；

• iso13484 certified manufacturer, registered with FDA；

 

Related products

Medical Nasal Swabs Kits Std Swab Virus Transport Medium

Nasopharyngeal Swabs Virus Sampling Transport Vtm Tube

Flocked Swab+Tube With Viral Transport Medium

Foam Swab VTM Kit

Contact us

Cell medical (Dongguan) Co,Ltd.

  • skype：+8618814476009
  • E-mail：flockedswabs@gmail.com
  • No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China
New Coronavirus nucleic acid detection

New Coronavirus nucleic acid detection steps

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New Coronavirus nucleic acid detection steps

1、 The specific operations are as follows

1. Virus sampling:

Take the patient’s saliva sample or nasopharyngeal flocked swab sample, and save the virus sampling tube.Virus preservation solutions commonly used in disposable virus tubes include inactivated and non inactivated types. The following are two models of virus transport medium:

1) Inactivated type: the inactivated virus transport medium characterized by killing the virus and retaining nucleic acid fragments. Application:detect COVID-19, influenza virus, hfmv and influenza A.

2) Non inactivated type: the non inactivated virus transport medium does not contain lysate. It can retain the activity and integrity of pathogens.Application:virus culture and reproduction to make the detection results more accurate.

flocked swab specimen collection_New Coronavirus nucleic acid detection steps

flocked swab specimen collection_New Coronavirus nucleic acid detection steps

2. Nucleic acid extraction:

Extract the genetic material from the patient’s sleeping fluid or nasopharyngeal swab sample. If a patient carries a virus, there will be RNA of the virus’s genetic material in the sample. Sampling personnel should pay attention to prevent RNA degradation when extracting RNA. At the same time, the extracted DNA shall be stored at – 20 ℃, and the RNA and DNA requiring long-term storage shall be stored at – 70 ℃ or liquid nitrogen.

3. Reverse transcription synthesis of cDNA:

reverse transcription of RNA in the extract, reverse transcription of RNA into cDNA, reverse transcription of cDNA synthesis reaction, using reverse transcription primers, dNTPs, reverse transcriptase, RNase inhibitor and DTT. Buffer, an appropriate amount of ultrapure water without RNA / DNase and RNA template. We propose to use commercially available RT-PCR one-step reagent for one round amplification of di.

4. PCR amplification reaction (nested PCR amplification method with secondary amplification):

Use specific primers of virus cDNA for PCR amplification in an amplification instrument. Nested PCR amplification using secondary amplification.

5. Result analysis and judgment:

If the DNA band of the virus is amplified, it is determined that the virus exists in the patient; If no DNA band is amplified, it is determined that the sample taken by the disease has no virus.

2、 Experimental precautions:

① We should set up two positive controls and two negative controls for each test. Only when the positive control enlarges the expected fragment, the negative control does not enlarge any fragment, and the results of two parallel samples are consistent.We can determine the nucleic acid positive or negative reaction results.

② Nucleic acid test is positive: if nucleic acid is positive, we should repeatedly collect samples for retest. If the retest result is nucleic acid positive, we can confirm that the sample result is nucleic acid positive. If the retest result is nucleic acid negative, the nucleic acid test result is uncertain.

Facing COVID-19 variation strain

Facing COVID-19 variation strain

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Facing COVID-19 variation strain. How should we deal with it？

What are the variant strains of COVID-19?

The variation of COVID-19 B.1.621 was first confirmed in Columbia in January this year. The World Health Organization named it Miao strain and recently listed it as a mutant strain that needs attention. Its influence level is lower than that of the delta strain. Preliminary data show that the Miao strain is similar to the beta strain and will weaken the effectiveness of the antibody.

At present, there are four variants defined by who as “needing attention”, including alpha, beta, gamma and delta. And five variants “needing attention”, including ETA, iota, kappa, lambda and mu.

How should we deal with it？

Weightier than Mount Tai, I think the most important thing about this new crown epidemic is: life is above everything else and responsibility is heavier than Mount Tai.

We often ask: where is the value of life? After this epidemic, we answer is: responsibility.

A person and an enterprise must have a sense of responsibility before their life can be valuable. If a person is not responsible and has no sense of responsibility, his life is worthless.

First, Let’s do a good job in personal protection. Don’t worry too much.

As the general public, we can prepare some masks, medical goggles,Rapid Antigen Test Kit,New Coronavirus test kits and so on.We advocate new crown nucleic acid testing at home

Rapid Antigen Test Kit specimen testing

Rapid Antigen Test Kit specimen testing

Cell medical (Dongguan) Co., Ltd. is a comprehensive high-tech biotechnology company and an OEM factory of flocking swabs in China.We support custom production,our factory produces flocked swabs, pharyngeal swabs, nasal swabs, oral swabs,Rapid Antigen Test Kit, virus sampling tubes, transportation media, sample preservation solution and nucleic acid extraction kit. Our GMP factory was built in 2016,the factory has ISO13485 quality management system, CE, FDA and other qualifications.

Secondly, we should keep regular work and rest and keep learning.

Adhere to exercise and treat your body: regular work and rest, balanced diet, exercise and regular physical examination. After all, the body is the capital of revolution, and immunity is the first competitiveness.

Finally, if your physical condition permits, please vaccinate as soon as possible. For you, for your family and for our national peace and security.

In order to better prevent COVID-19 variation strain, Cell medical (Dongguan) Co.,Ltd. increases production and guarantees supply.

Flocked swabs can be customized

Flocked swabs can be customized according to customer needs

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Flocked swabs can be customized according to customer needs.

Flocked swab customization

Cell medical (Dongguan) Co., Ltd. is a comprehensive high-tech biotechnology company and an OEM factory of flocking swabs in China. We support custom production, our swab OEM factory produces flocked swabs, pharyngeal swabs, nasal swabs, oral swabs, sponge swabs, virus sampling tubes, transportation media, sample preservation solution and nucleic acid extraction kits. Our GMP factory was built in 2016, the factory has an ISO13485 quality management system, CE, FDA and other qualifications.

vtm kit production process

vtm kit production process

flocked swabs customization

flocked swabs customization

We support ODM and OEM

Our factory has many automatic production lines. We support customized production, OEM, ODM, etc., and develop and design products with customers. Willing to challenge all kinds of demanding swabs.

Flocked swab customization, VTM kit customization, virus transport medium customization, virus collection and transport kit customization, disposable virus sampling tube customization, nasopharyngeal swab customization, oropharyngeal swab customization etc.
Welcome to consult our factory, we will serve customers wholeheartedly and develop high-quality flocking cotton swabs with customers.

Product features

1. nylon fiber flocked swab technology, the sample collection amount and release amount are as high as 95%;

2. nylon staple fixed vertically, faster and more efficient elution;

3. Single and independent packaging shall avoid pollution.

4. Strict process conditions, without DNase and expandable human DNA.

5. The tube body is transparent and the inspection materials are visible.

6. The unique patented casing design ensures the air circulation in the casing, prevents the inspection material from mildew due to the humid sealing environment. And avoids magazine pollution in the external air, which affects the inspection quality.

Our advantages

1. specially designed for virus sampling and DNA micro-sample. Especially for micro samples such as sweat, semen, blood, dandruff and oral cells, it has superior collection performance.

2. It can quickly adsorb trace samples and has a high release aging rate.

3. the front end is more sophisticated, which is suitable for the extraction of cells in the fingernail seam of the victim or suspect.

4. Unique swab breakpoint design, which is conducive to the automatic extraction workstation for sample extraction.

5. The whole swab is packed in a transparent plastic tube to avoid biological pollution.

 

 

 

VTM Factory VTM kits with flocked swab for sample collection

VTM Factory VTM kits with flocked swab for sample collection

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VTM Factory VTM kits with flocked swab for sample collection.

About us_VTM Factory

Cell medical (Dongguan) Co., Ltd., is a professional manufacturer of  VTM kits , more than 10 years of experience in medical application molding and flocked treatment heads and swabs. Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability. Certificate:FDA,CE,EU,Free Sale,Export license based on (GMP).

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production process VTM Factory

production process VTM Factory

Viral Transport Medium kits

Viral Transport Medium kits

VTM Factory FAQ

Q1: Why choose us?
A1: a professional manufacturer of  VTM kits, more than 10 years of experience in medical application molding and flocked treatment heads and swabs.Excellent and stable quality with best cost performance.Various payment term available.Timely delivery guaranteed.Supply Ability:500,000-1,000,000 Pieces per Month.Q2: Can I visit your factory?
A2: Sure, warmly welcome your visit . Please try to contact our sales representative first by email or phone. We will make the appointment and the most reasonable planning for your transportation.

Q3: Can you supply free samples?
A3: Yes, we would like to offer you free samples with freight collect.

Q4: Can you offer customized service?
A4: Yes, both OEM and ODM are available. Let me know your idea or requirements on the products, we make it perfect for you.

Q5: How long is your delivery time?
A5: Generally 20-30 days for mass production after sample confirmed and the receipt of formal PO or deposit.

How to use cm-98000 oral swab

How to use cm-98000 oral swab

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How to use cm-98000 oral swab

Brief description

A large number of clinical experiments on cell medical (Dongguan) Co, Ltd. nylon flocked swab show that CM nylon flocking swab has better collection and transportation effect on clinical microbial samples than ordinary sterile cotton swab. This is especially true for those specimens that cannot be submitted for examination in time and placed for too long.

cm-98000 oral swab

cm-98000 oral swab

advantage

1. The unique jet nylon fiber implantation technology increases the collection and release of specimens.

2. The total length of the swab is 14.5cm, and the plastic rod has a unique breakable design.

3. The villus texture can collect more target analytes.

4. There is no residual specimen, so the specimen processing process can be accelerated.

5. Swabs are sterilized and packaged separately

How to use cm-98000 oral flocked swab

① Tear the sealing paper on the disposable oral swab package, and gently take out the disposable sampling swab from the bag（ Note: the head of disposable oral swab shall not touch other objects other than the inner wall of oral cavity to avoid contamination.)

② . extend the hand-held sampling swab into one side of the oral cavity, from inside to outside at the inner wall mucosa, and then move the swab up and down for 15-20 times. The number can be increased according to the actual situation, with moderate strength. It is appropriate to stick close to the inner wall mucosa of the oral cavity, so as to ensure that the one-time sampling swab head can dip in the exfoliated cells of the oral mucosa everywhere; According to the same method, collect on the other side of the inner wall of the oral cavity.

③ After collection, please take out the disposable sampling swab from the mouth and put it into the sampling tube, push the disposable sampling swab head, put the sampling head into the storage tube, tighten and save it（ Note: too many rotations will lead to dilution or loss of samples. After sampling, make sure that the disposable sampling swab does not touch any other object.)

ABOUT US

Cell medical (Dongguan) Co.,Ltd., a swabs design / swabs manufacturer, has many years of experience in medical application molding and flocking treatment heads and swabs.

Main products:Flocked Swabs,VTM Kits,Viral Transport Medium,VIRUS Collection & Transport Kits,Disposable virus sampling tube,Op swabs,Np swabs,etc.

Contact us

 

how to collect nasopharyngeal swab

how to collect nasopharyngeal swab?

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how to collect nasopharyngeal swab?

After several times of nucleic acid sampling and testing, citizens wondered: why do some people require nasopharynx swabs and some people can collect oropharyngeal swabs?  Today, experts from cell medical will explain to you.

What is a sampling swab?

At present, except for special diseases, COVID-19’s nasopharyngeal swabs and oropharyngeal swabs are the most common ways of collecting.

Studies have shown that nasopharyngeal swabs have higher detection rate of COVID-19 nucleic acids than oropharyngeal swabs. Because the nasopharyngeal cavity is relatively closed and the virus is easier to accumulate. However, whether it is oropharyngeal swab or nasopharyngeal swab, the key is that the collection action must be standardized, in place and meet the standards. As long as the acquisition is standardized, both methods can achieve the expected detection purpose.

How to collect a nasopharyngeal swab?

nasal swab collection method_How to collect a nasopharyngeal swab？

nasal swab collection method_How to collect a nasopharyngeal swab？

1. The patient’s head tilts back (about 70 degrees) and persists. 2. Use a swab to estimate the interval from the ear root to the nostril. 3. Insert from the nostril pen to the face. The deepening interval should be at least half of the length from the earlobe to the tip of the nose. Reach the posterior nasopharynx after encountering resistance, stay for several seconds to absorb secretions (generally 15 ~ 30s), and rotate the swab for 3 ~ 5 times. 4. The swab should be gently rotated and taken out, and the swab head should be immersed in a collection tube containing 2ml lysate or cell preservation solution containing RNase inhibitor. 5. Break the sterile swab rod at the top, discard the tail, tighten the tube cover and close it with sealing film.

oropharyngeal swab collection

collection method of oral swabs

collection method of oral swabs

1. Ask the patient to gargle with normal saline or clear water first.

2. Wet the flocked swab in sterile normal saline.

3. The patient sat down with his head tilted back and his mouth open, accompanied by an “ah” sound.

4. Fix the tongue with a tongue depressor, and the swab jumps over the root of the tongue to the posterior pharyngeal wall, tonsil recess, lateral wall, etc.

5. The bilateral pharyngeal tonsils should be swabbed back and forth with moderate force for at least 3 times, and then the posterior pharyngeal wall should be swabbed for at least 3 times, 3 ~ 5 times.

6. Remove the swab to prevent touching the tongue, pituitary, oral mucosa and saliva.

7. Immerse the swab head into the preservation solution containing 2 ~ 3ml virus.

8. Break the sterile swab rod near the top, discard the tail, tighten the tube cover and close it with sealing film.

After the introduction of our experts, do you have a deeper understanding of sampling swabs? If you want to know more product details and prices, please leave us a message. We will serve you wholeheartedly!

Contact us

    what is a flocked swab

    What is a Flocked Swab

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    what is a flocked swab

    Many customers ask me, why is use flocking sampling swabs? You’ll know from the comparison below：

    The flocked sampling swab of cell medical (Dongguan) Co, Ltd. is composed of nylon short fibre velveteen wool head and medical-grade ABS plastic rod. The volume of samples collected and released is 3 times higher than that of traditional swabs.

    Ensure the survival rate of cell samples, no residual fatty acids, and will not affect the test results.

    cm flocked swabs Display diagram

    cm flocked swabs Display diagram

    The sampling swab set contains a swab and a cell preservation solution. After collecting samples with swabs, we stored them in a liquid-based cell preservation solution. It ensures the integrity of sample DNA,

    How are flocked swabs made?

    Made of nylon fibre flocked technology, and the front end with nylon short fibers fixed in a vertical manner.
    So that there is no absorption hole in the sampling area of ​​the sampling swab.
    The sample we obtained with a cotton swab does not disperse and remains in the fiber
    Which is beneficial Faster and more efficient elution.
    Traditional cotton swabs cannot completely collect and elute samples while flocking swabs can elute 95% of samples.
    Therefore, the use of flocked swabs has great advantages for various sample collection applications.

    What is a flock nylon swab?

    Nylon flocking sampling swabs are specially used for sample collection.
    And cotton swabs can also be used for sample collection, but the number of samples collected and the collective effect of the two cannot be compared.
    Flocked nylon swab with outstanding sample collection and release capabilities.
    It can quickly absorb trace amounts of samples, and the release efficiency is high.
    The increase in the number of targets helps to improve the sensitivity of rapid diagnostic tests.

    product features:

    • flocking swab has a good sampling effect, and the sample collection amount and release amount are as high as 95%;
    • our unique technology increases the comfort of the collected;
    • ethylene oxide sterilization has no inhibition on the subsequent application;
    • swab using spray dense planting nylon fiber technology;
    • nylon fiber swabs can better collect clinical samples and immediately release them into the liquid delivery medium.
    • It has an ABS rod with a “breakpoint“.

    In short, the upright nylon fiber is like a soft brush, which can collect more cell samples. Nylon flocked swabs can absorb and release more samples.

    How do you use a Copan swab?

    What are flocked swabs used for?

    cytological sample processing, virological cell culture, DFA test, enzyme-linked immunosorbent assay, PCR test, molecular diagnosis, epidemic disease detection, etc.

    1. Disposable sampling swabs for oral cavity and nasopharyngeal swab.

    2. Gynecological sampling swab.

    3. Virus and bacteria sampling swab.

    4. Disposable DNA sampling swab.

    5. Cell sampling sterile swab.

    6. Laboratory test sampling swab.

     Our flocked swabs are famous for their stable and reliable performance and excellent absorption and elution ability.

    • E-mail：flockedswabs@gmail.com
    • Welcome to consult, We will serve you wholeheartedly.
    • Cell medical (Dongguan) Co., Ltd.
    • China swab and VTM kits manufacturers
    VTM virus sampling tube kits

    VTM viral transport medium tube

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    VTM viral transport medium tube_virus sampling tube-universal transport medium

    1.[information]

    1. Common name: disposable virus sampling tube.

    2. Product Name: universal transport medium.

    3. Specifications and models vtm-a-cm and vtm-b-cm series.

     2.[package specification]

    20 copies / box.

    VTM viral transport medium tube-disposable sampling kits

    VTM viral transport medium tube-disposable sampling kits

    3.[VTM use]

    For sample collection, transportation and storage.

    4.[viral transport medium tube test principle]

    On the basis of Hank’s, potassium chloride, magnesium chloride and calcium chloride are added to provide necessary nutrient ions for pathogenic bacteria to make it more specific. The preservation time is longer, the integrity of pathogenic bacteria morphology is preserved to a greater extent, and the extraction rate of pathogenic bacteria is improved.

    5. [structure composition ]

    flocked swab and virus transport medium.(or universal transport medium).

    6. [sample requirements]

    the collected samples shall be sent to the laboratory within 48 hours at 2-8 ℃. If they cannot be sent to the laboratory within 48 hours, they shall be stored at – 70 ℃ or below. The samples shall be inoculated and separated as soon as possible after being sent to the laboratory.

    7.[storage conditions and validity period]

    1. The products shall be stored in a clean, hygienic, dry and ventilated environment;

    2. The temperature is 5 ℃ ~ 35 ℃. 3. Relative humidity ＜ 85% RH. 4. Product shelf life: 12 months.

    universal transport medium and flocked swabs-vtm swab

    universal transport medium and flocked swabs

    8.[usage]

    Firstly,before sampling, mark relevant information on the label of the sampling tube.

    Secondly,use the corresponding sampling flocked cotton swab for sampling.

    Then,after sampling, quickly put the cotton swab into the sampling tube, break the part higher than the sampling tube, and tighten the tube cover.

    Finally,see the following for specific sampling methods.

    a)Nasal swab: gently insert the sampling head into the nasopalatine part of the nasal catheter, stay for a moment, and then rotate it slowly to exit. Immerse the collected specimen into the liquid in the sampling tube, break the excess part and discard it, and tighten the sampling tube cover.

    b) Pharyngeal swab: wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with sampling head. Soak the collected specimen in the sampling solution, break the excess part, discard it, and tighten the sampling tube cover.

     9.[precautions]

    1. After collecting pathogenic bacteria samples, we should completely insert the disposable sampling flocked cotton swab into the preservation solution to retain pathogenic bacteria to the greatest extent.

    2. The collected samples must be fresh and sent for examination in time to avoid the death of pathogenic bacteria.

    3. Forbidden to use products with damaged packaging and expiration date to prevent pollution.

    Note: For in vitro diagnosis. For virus sampling, transportation and storage.Including COVID-19.

    Food can lead to serious events; It has certain irritation to eyes and skin. If it accidentally splashes into eyes, rinse with clean water.

    10.[Manufacturer information]-VTM virus sampling tube kits

    [name of manufacturer] cell medical (Dongguan) Co, Ltd .

    [address] No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China .

    [E-mail]：flockedswabs@gmail.com

    DO you to know about the virus sample preservation solution

    CM company take you to know the virus sample preservation solution.

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    Cell medical (Dongguan) Co, Ltd.,Take you to know the virus sample preservation solution.

    Virus sample preservation solution,also known as disposable virus sampling tube.When we first came into contact with this term, we must be very curious.

    However,what is  DNA / RNA virus sampling tube？

    First of all, virus sampling tube is a liquid that protects the tested substance of the virus by immersing the sampling swab virus sample in the sampling tube. Currently, it is mainly used for the collection, storage and transportation of common virus samples such as COVID-19, HFMV and so on. Medical workers can take tissue samples from the throat or specific areas using throat swabs and nasal flocked swabs. Samplers then store the samples for subsequent clinical trials such as nucleic acid extraction or purification. The virus and related samples can be stored at -70℃ or liquid nitrogen for a long time.

    Usually,we divide the virus storage solution into two kinds. the non-inactivated virus storage solution and the inactivated virus sampling tube. The non inactivated type can protect viral proteins and nucleic acids. Maintain virus activity and facilitate culture and detection. Although the inactivated type can make the virus no longer have physiological activity, lose infectivity and pathogenicity, and have the ability to reproduce. Inactivate the virus, eliminate secondary infection,and protect the safety of transportation and testing personnel.

    disposable virus sampling tube

    disposable virus sampling tube

    About inactivated sample preservation solution.

    There may be a small partner who will question whether the inactivated type will inactivate the virus? How do you check it? Don’t worry, the purpose of using inactivated virus sampling tube is to lyse nucleic acid and detect nucleic acid by subsequent RT-PCR.

    After extraction with most commercial kits, the quality of DNA or RNA obtained is good and the yield is high. So,it can complete various gene detection and analysis experiments, such as PCR or qPCR, and save transportation costs.

    It is suitable for COVID-19, hfmv and other common virus samples.

    About inactivated sample preservation solution.

    • The virus preserved in non inactivated virus sampling tube has better integrity and higher detection rate. Researchers use it for scientific research, including nucleic acid testing.
    • Cryogenic storage without freezing, does not destroy the virus shell, easy long-distance transportation;
    • Application:H1N1 influenza virus and any other Virus swab sampling;
    • The antibiotics in the preservation liquid can effectively prevent bacterial and fungal infection;

    Contact us