How to Obtain a Nasopharyngeal Swab Specimen

How to Obtain a Nasopharyngeal Swab Specimen

How to Obtain a Nasopharyngeal Swab Specimen

As a non-invasive operation, nasopharyngeal swab sampling is the first choice for diagnosis, monitoring and differential diagnosis of New Coronavirus pneumonia. Its sampling randomness is high. However, the sampling personnel may have false-negative test results due to the different location, strength and time of scraping samples, which may have an adverse impact on the epidemic prevention and control. During the operation, the patient choked and splashed to produce an aerosol, which is very easy for medical staff to have occupational exposure.

This paper mainly explains in detail the protection of medical staff when collecting nasopharyngeal swabs, the collection, packaging and transportation of nasopharyngeal swabs, as well as the patient’s body position, operation methods, collection parts and precautions during specimen collection.

Effective communication and nurse-patient cooperation help the successful collection of nasopharyngeal swabs.

Before the operation, the degree of cooperation of patients should be effectively evaluated. Inform the patient of the collection purpose and precautions in advance.

Clinicians should be cautious if the patient has recent nasal trauma or surgery, a significant deviation of the nasal septum, or a history of chronic obstructive nasal patency or severe coagulation disease.

Standard protection is the foundation of successful nasopharyngeal swab collection

Operator preparation: first, wear protective clothing, wash hands with soap and water (or alcohol), and wear a pair of non-sterile gloves. Then, according to the recommendations of the Centers for Disease Control and prevention, wear an N95 or higher protective mask. Finally, wear a face mask to protect your face and eyes.

Swab Nasal Covid Sampling preparation: operating platform; Sampling materials, including nasopharyngeal test swab, VTM kits, inspection sheet and bar code, transport bag and hand disinfectant, to ensure the sterilization effect and validity period.

 Obtain a Nasopharyngeal Flocked Swab Specimen

Obtain a Nasopharyngeal Flocked Swab Specimen

Nasal swab specimen collection method.

(1) Wet the flocked swab in normal saline.

(2) Measure the distance from nostril to ear root with a swab and mark with your fingers.

(3) Insert the swab into the nostril in the direction perpendicular to the nose (face) until the finger touches the nose, keep the swab in the nose for 15-30 seconds, and then rotate it gently for 3 times.

(4) Put the swab into the virus delivery medium, break the swab rod and place it completely in the tube.

(5) Tighten the tube cover, mark it, put it into the specimen bag and seal it.

(6) If it is necessary to collect from two nostrils, one swab should be used respectively.

(7) Store at 4 C or on ice (short term storage).

CDC recommends that all patients suspected of infection with COVID-19 use masks.

Ask the patient to take off the mask and wipe the nose with a paper towel to remove excessive secretions from the nasal cavity.

Remove the cotton swab from the package. Tilt the patient’s head back slightly so that the nasal tract is more accessible.

Let the patient close his eyes to reduce the slight discomfort caused by the operation.

Gently insert the swab into the nasopharynx along the nasal septum until resistance is felt.

Tips for nasopharyngeal swab sample collection.

The nasopharynx swab is inserted quickly. Do not hesitate. Some patients are intolerant to the operation of probing the nasopharyngeal swab into the nasal cavity, resulting in tears, foreign body feeling, etc. before the operation, ask the patients to inhale deeply in order to avoid choking and coughing.

When the cotton swab reaches the expected depth, do not continue to go deep, so as not to cause nasal mucosal damage and bleeding. During the stay, use finger strokes with the patient to read the seconds. It can distract attention, eliminate tension and motivate patients to ensure successful collection.

Never allow the patient to fully raise his head and bend the swab, which will lead to incorrect retention position of nasopharyngeal swab head and ultimately affect the accuracy of specimen collection results.

The operator can select the left / right side of the operator according to his left and right hands, and stand on the patient’s head and shoulder for operation. The patient’s head does not need to be biased to the opposite side. It is difficult to control the entry angle of the flocked swab when facing the side head, which may cause mucosal irritation. For patients with excessive tension, the operator can hold the patient’s hand and give comfort.

Quick questions and answers about nasopharyngeal swab sampling

1. Q: can carry out New Coronavirus test specimen collection personnel?

A: Qualified personnel with sampling skills after biosafety training.

2. Q: COVID-19 test specimen preferred?

A: Respiratory specimens.

Specific detection of

3. Q: infection in New Coronavirus includes?

A: Nucleic acid detection (nasal swab and pharyngeal swab); Virus isolation; Antibody detection.

4. Q: What are the key points of nasal swab collection?

A: Nasal swab: when the top of the swab reaches the posterior wall of the nasopharyngeal cavity, rotate it gently for one week.

5. Q: what respirator is used to collect COVID-19 respiratory specimens?

A: Masks N95 and above.

6. Q: how to disinfect hands after collecting samples?

A: After disinfection with disinfectant, take off the outer gloves, disinfect the inner gloves and wear new outer gloves.

7. Q: when taking off personal protective equipment, which of the following operations should be carried out first?

A: Spray disinfectant all over the body.

8. Q: What should we pay attention to when taking off conjoined protective clothing?

A: Ensure the contaminated surface of the conjoined protective clothing in the whole process, do not touch the inner clothing, and take off slowly and gently.

Contact us

Cell medical (Dongguan) Co., Ltd.
E-mail:flockedswabs@gmail.com
No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China.
Web:www.swabmanufacturer.com

Sterile Flocked Nylon Swab with 80mm Breakpoint

Sterile Flocked Nylon Swab with 80mm Breakpoint

Sterile Flocked Nylon Swab with 80mm Breakpoint.

Flocked swabs consist of nylon fibres to provide not only excellent sample collection but also release of that sample into Transport media.

The term “flocked” refers to a process of attaching fibers of variable length flocking to a surface coated in the adhesive in order to enhance sample collection.

Flocked swabs provide advantages for a variety of specimen collection procedures employed by medical and laboratory professionals. The swab is designed to be ergonomic for the user as well as for patient comfort and to optimize sample collection.

The flocked nylon fibers act similar to a fine bristled brush, which creates thousands of contact points whereby, cells, microscopic organisms or particles may be collected. Sterile flocked swabs are the preferred choice when collecting samples from the nasal cavity.

Nasal Swab flocked swab with 80mm breakpoint

Nasal Swab flocked swab with 80mm breakpoint

Get Latest Price

Email:flockedswabs@gmail.com

Product parameters.

Nasopharyngeal swab, 80 mm

100 swabs per package

Application type – nasopharynx

sterile

Nylon flocking tip. ABS plastic shaft

Needle tip diameter length: 2.5cm; Width 1 mm; The total length of the swab is 15 cm

Medical Swab Test Kit specimen collection sterile nylon flocked swab

Medical Swab Test Kit specimen collection sterile nylon flocked swab

Sterile Flocked Nylon Swab performance and structure.

Composed of a swab head (flocked nylon plush head) and a polystyrene plastic rod.

Sterile (micro) plastic rod nylon flocked swab (separately packaged) for nasopharynx.

For one-time use and sterilized by radiation.

It is suitable for DNA sampling, nasopharynx and other parts of the sample collection, convenient transmission, avoid pollution.

Name: nylon flocked swab for nasopharynx, plastic rod, aseptic independent packaging.

This product is produced by new technology in Australia. A large number of clinical trials abroad show that compared with ordinary sterile swabs, nylon flocking swabs have better collection and transportation effects on clinical biological samples, especially for those samples that cannot be submitted for inspection in time and placed for too long.

Flocked Nylon Swab Advantage:

1. Connection strength: when the moving speed of the fixture of the tensile testing machine is 200 mm / min; The pull-out force between the sampling head and one end of the connecting rod shall not be less than 2n,

2. Breaking strength: when the test span is 6cm, the swab shall be able to withstand 4N axial static pressure for 15s without permanent deformation or fracture phenomenon.

3. Rotational friction degree: spray nylon fluff on the ABS rod after viscose. The rubbing fastness refers to that the test is not greater than 2n, the rotational friction is not less than 3 times at 360 degrees, and the amount of nylon fluff on the surface is not greater than 2.

The sterile disposable sampling flocked swab has passed the whole project testing by the national quality supervision instrument testing centre, and the qualified test report zc1011965 / 96 / 97 has been issued.

The sampling swabs are divided into pharyngeal swabs, oral swabs, DNA swabs and cervical swabs, which can be used by various laboratory testing centres.

The sampling flocking swab is three times higher than the traditional winding swab in the number of samples collected and released and does not cause damage to the cell sample, so as to ensure the survival rate of the cell sample, no residual fatty acids, no impact on the test and detection effect, and no harm to the human body.

*Nasal swab for medical institutions to collect virus and DNA samples infected in the patient’s nasal cavity;

Sterile Flocked Nylon Swab application

Sterile Flocked Nylon Swab application.

*Type an oral swab for medical institutions to collect virus and DNA samples of patients with oral and throat infections;

oral swab

oral swab.

viral transport medium with swab

Viral Transport Medium with Swab

viral transport medium with swab | COVID-19 sampling and collection kits

The epidemic has disrupted the whole world and affected many industries.

During the outbreak of  COVID-19, people were paying close attention to the epidemic situation while doing personal protection. Novel coronavirus pneumonia and influenza were the most frequent period of communication in winter and spring. The most important way to control the epidemic is to select new patients with new crown pneumonia from many respiratory patients.

About viral transport medium with swab.

Swabs and virus transport media are a transport and collection system for long-term freezing, transport and collection of clinical specimens containing viruses.

The main species of organisms usually transported through this medium include chlamydia, urea plasma organisms and mycoplasma.

With the outbreak of COVID-19 pandemic, swabs and virus transport mediums were used to freeze and collect SARS-CoV-19 virus samples.

Rub a swab on the skin or vesicles to collect virus samples. The collected samples are then sent to the laboratory with transport media.

Why do swabs need virus transport media

After virus collection, the traditional method can not be detected by PCR immediately. The virus itself will quickly dissolve in vitro and affect subsequent tests.

viral transport medium with swab -vtm kits

viral transport medium with swab -vtm kits

Purpose of virus transport media:

1. For monitoring and sampling infectious pathogenic microorganisms by disease control departments and clinical departments. It is applicable to the sampling of influenza virus (ordinary influenza, highly pathogenic avian influenza, influenza A H1N1 virus, etc.), hand, foot and mouth virus and other types of viruses.

2.For the sampling of mycoplasma, chlamydia and Ureaplasma.

3.. To transport nasopharyngeal swab samples or tissue samples at specific parts from the sampling site to the detection laboratory for PCR extraction and detection.

4.To preserve nasopharyngeal swab samples or tissue samples at specific sites for necessary cell culture.

Precautions for sample transportation.

1. Inspection time and temperature control:

After samples sampling, for inspection as soon as possible and sent to the laboratory within 2 ~ 4H as far as possible.

Transport at 2 ~ 8 ℃, and the transport time shall not exceed 72h. If it exceeds 72h, stored and transported at – 70 ℃ or lower.

2. Viral transport medium transport container:

Virus transport tube:waterproof, damage proof, leak proof, high temperature (low temperature) and high pressure resistant. 
Tube body: biological hazard signs, warnings and reminders with relevant regulations. 
Transport container: three-layer packaging system, namely inner packaging, middle packaging and outer packaging.

3. Transportation in the hospital:

The specimen delivery personnel shall be provided with secondary protection and carry 75% ethanol with them.

4. Long distance transportation:

The transport package of 2019 ncov specimens belongs to class A, and the corresponding UN number is un2814. The transporter shall wear safety protection according to the requirements of class II protection, and carry 75% ethanol with him. Drivers wear surgical masks or N95 masks and transport them by special vehicles. If transported by air, the packaging shall also comply with the pi602 classified packaging requirements of ICAO document Doc9284-AN / 905 technical rules for safe air transport of dangerous goods. At least one specimen transporter and driver shall transport specimens at the same time, and monitoring facilities for specimen transport process shall be equipped.

Contact us

  • Cell medical (Dongguan) Co., Ltd.
  • skype:+86-18814476009
  • E-mail:flockedswabs@gmail.com
  • No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China
  • Web:www.swabmanufacturer.com/

 

Flocked Swab Meaning

Flocked Swab Meaning

Flocked Swab Meaning

This article will introduce what is the flocked swab, the purpose of the flocking swab, the application of the COVID-19 flocked swab and the material of the flocked swab, etc. The flocked swab manufacturer introduces. Give you a comprehensive understanding of flocking swabs.

what is the flocked swab?

Flocked Swab Meaning

Since the outbreak of COVID-19, the word “nasopharyngeal swab” has become a high-frequency word we talk about every day.

In recent months, the new crown pneumonia epidemic broke out again in countries all over the world. It’s worrying to watch the data released by WHO every day!!!

Flocked swab material

The flocked swab is made of nylon fiber flocking technology, and the front end is coated with nylon short fiber fixed vertically so that there is no absorption hole in the sampling area of the whole sampling swab, and the sampling sample will not be dispersed and retained in the fiber, which is conducive to faster and more efficient elution.

Traditional cotton swabs can not completely collect and elute samples while flocking swabs can elute 95% of samples. Therefore, the use of flocking swabs has great advantages for various sample collection applications.

The flocked swab is to deposit nylon fibres on the surface of the medical ABS rod in a certain direction through an electrostatic field to form a brush layer.

What is the purpose of flocked swabs?

Flocking swabs are the best choice for nucleic acid detection because they can better collect cell or biological samples at the collection site and release samples quickly.

Application: forensic medicine, DNA, microorganism, virus sampling, Oral swab, nasal swab etc.

What are flocked swabs used for in COVID-19?

The autumn and winter communication season is a period of the high incidence of common cold and influenza, and the most important means to control the epidemic situation is to select real patients with new crown pneumonia from a large number of respiratory tract patients.

At present, the most effective and widely used new crown sampling methods are nasopharyngeal swab and oropharyngeal swab sampling.

Flocked Swab Meaning _Application of flocking swab in COVID-19

Flocked Swab Meaning _Application of flocking swab in COVID-19

Nasopharyngeal swab collection method.

1. Keep the patient’s head still and remove the secretions on the middle surface of the anterior nostril;

2. Measure the distance from the nose tip to the earlobe with a swab and mark it with your fingers. Insert the swab into the nasal cavity in the direction perpendicular to the nose (face). When encountering resistance, it reaches the posterior nasopharynx, keeps the swab in the nose for 10 ~ 15 s, rotates it gently for 3 ~ 5 times, and take out the test flocked swab.

According to the American Society of Microbiology guidelines for the submission of clinical microbial specimens, the nasopharyngeal swab should be twisted on the nasopharyngeal mucosa, retained for 10-15 seconds, and then removed.

Feeling of nasal flocked swabs sampled by U.S. netizens.

netizens 01

netizens 01

It’s hard, and the tough guy still cries.

I have experienced both sampling methods.

It is not a painful cry, but a stress response caused by strong stimulation of the weak nasopharyngeal mucosa, with tears and tears.

netizens 02

netizens 02

No, as a girl, I don’t feel much about the nose swab sampling, except its excitement went to my head.

netizens 03

netizens 03

The more serious one is like eating mustard oil, and the lighter one is like blowing out your nose for several days.

Our netizens are so cute.

About oropharyngeal swab sampling.

oral swab sampling process_ Flocked Swab Meaning

oral swab sampling process_ Flocked Swab Meaning

Feeling of oral flocked swabs sampled by China's netizens.

Feeling of oral flocked swabs sampled by China’s netizens.

Pharyngeal(oral) swab: the collection process is faster (just a few seconds) and less painful, but you may feel nausea and vomiting, and some people have no obvious reaction.

As we are in the epidemic period, we should improve our awareness of self-protection when doing nucleic acid testing.

Flocked swab manufacturer introduces Cell medical (Dongguan) Co., Ltd.

Cell medical (Dongguan) Co., Ltd. is a comprehensive high-tech biotechnology enterprise.

We have invested a lot of scientific research strength to tackle key technical problems.

At present, our virus preservation tube and flocked swab have shown an irreplaceable role in the detection of new infectious diseases.

We support custom production, OEM and so on, and develop products with customers.

Welcome to leave a message for a consultation!

CONTACT US

What is a Nasopharyngeal Swab?

What is a Nasopharyngeal Swab?

What is a Nasopharyngeal Swab?

COVID-19 Nucleic acid swab test  is a very effective and effective measure to prevent and control the new crown pneumonia epidemic.

The upper respiratory tract of human body includes nasal cavity, pharynx and throat.

That is to say, if people are infected with COVID-19, they can extract the nucleic acid of the virus in the nasal cavity, pharynx and larynx in 96 hours.

Therefore, we usually sample these locations in the rapid detection of new crown nucleic acids.

How to collect a nasopharyngeal swab?

Nasal swab sampling process

The first step is to collect secretions.

First, the medical staff will use a pharyngeal swab to wipe the subject’s nasal cavity or throat tonsils to collect salivary secretions.

nasal swab collection method

nasal swab collection method

The second step is to reserve samples.

After collecting the sample, immerse the nasopharyngeal swab in the preservation solution and screw on the tube cover to retain the sample.

The third step is to send samples for inspection.

Then, put the samples into clean sealed bags and send them to relevant parts for testing.

The fourth step is nucleic acid extraction.

Relevant departments will send the samples to the designated laboratory for nucleic acid extraction experiment.

Step 5: fluorescent PCR nasopharyngeal swab.

After collecting nasal samples, the experimenter uses fluorescent PCR to detect whether there is amplification reaction.

That is, to judge whether it is negative or positive.

Step 6:Finally, Receive test report.

Finally, according to the fluorescent PCR reaction results of nucleic acid detection, the nucleic acid detection vehicle inspection report is obtained, and I can query the results.

Sampling swab material

The sampling swab used for nasopharynx collection consists of a narrow rod made of a short plastic rod, one end of which is covered with an absorbent material. Such as cotton, polyester or flocked nylon.

The swab used for virus sampling should directly contact the sampling part.

The material of the sampling head is closely related to subsequent detection.

And the material requirements of virus sampling swab will be very high.

The virus sampling swab head shall be made of polyester (PE) synthetic fiber or artificial fiber.

Forbidden to use calcium alginate sponge or wooden swab (including bamboo stick).

However, calcium alginate and wood components can also absorb proteins after immersion in the preservation solution, and even inhibit the subsequent PCR reaction.

What are the advantages of using flocked sampling Nasopharyngeal swabs?

1. Flocked swabs provide better sampling.

Flocking swabs are a good choice for rapid diagnostic tests because they can better collect cells or organisms at the collection site and ensure the rapid release of the whole cell.

2. Enhanced diagnostic sensitivity.

Professional flocked technology turns each fiber into a velvet like swab, thus creating hundreds of thousands of contact points to better collect and release the collected samples.

Compared with non aggregated swabs, the increase in the number of target cells helps to improve the sensitivity of rapid diagnostic tests.

3. Rapid elution.

Their stable and reliable performance and excellent absorption and elution ability.

4. Easy handling and transportation.

sterilized and packaged separately, and the sterile swabs.

 

 

 

China Sterile Sampling Swab

China Sterile Sampling Swab factory and manufacturers

China Disposable Sterile Sampling Swab factory and manufacturers

Guangdong Dongguan city Changan town comprehensive high-tech biotechnology company -Cell medical (Dongguan) Co., Ltd. , Nissan large amount of flocking sampling swab, Disposable Sterile Sampling Swab help COVID-19 prevention and control.

Our GMP factory _Cell medical (Dongguan) Co., Ltd.

Our GMP factory _Cell medical (Dongguan) Co., Ltd.

Controlled environment Labor uniform strict control, Ensure DNA& RNA free product

Controlled environment Labor uniform strict control, Ensure DNA& RNA free product

 

Brief description

China Sterile Sampling Swab floced swab

China Sterile Sampling Swab floced swab

Aseptic environment sampling swabs include aseptic environment sampling swabs, disposable sampling swabs, aseptic sampling swabs, disposable aseptic environment sampling swabs and disposable aseptic sampling swabs.

sterilization and packaging method

1. Sterilize the product with ethylene oxide

2. Adopted international paper plastic packaging convenient for sterilization.

3. Independent packaging, easy to use.

special storage conditions and methods

Stored in a cool, dry and clean environment with no corrosive gas at 2 ℃ ~ 30 ℃ and relative humidity of 40% ~ 90%.

Nasal swab: for medical institutions to collect virus and DNA samples infected in patients’ nasal cavity. Virus sampling swab: used for nose and throat sampling of respiratory and intestinal viruses such as influenza, swine influenza, avian influenza, hand, foot and mouth.

Product composition: ⑴ nylon flocked swab. That is, sterile sampling swab (for sampling, the release is up to more than 90%).

 Product features:

① International paper plastic packaging convenient for sterilization.

② γ Ray sterilization to ensure sterility.

③ Each set of independent packing in the large packing box is convenient to use.

④Researchers can select different culture media (bacteria, viruses, mycoplasma, chlamydia) for different types of specimens.

China Sterile Sampling Swab (Cell medical ) advantages:

1. Collection swab features: the collection system adopts nylon flocked swab, which is non-toxic to microorganisms and can maximize the collection and release of samples.

2. A large number of clinical experiments show that compared with ordinary sterile cotton swabs, nylon flocked swabs have a better effect on the collection and transportation of clinical microbial samples.

Especially for those samples that cannot be submitted for examination in time and placed for too long.

Advantages of flocked swabs:

① The unique jet nylon fiber implantation technology increases the collection and release of specimens.

② The total length of the swab is 15cm, and the plastic rod has a unique breakable design.

③ Flocking swabs can collect more target analytes.

④ There is no residual specimen, so the specimen processing process can be accelerated.

⑤ Swabs are sterilized and packaged separately. Aseptic swabs packaged separately.

Is anal swab nucleic acid test more accurate than nasopharyngeal swab?

Is anal swab nucleic acid test more accurate than nasopharyngeal swab?

Is anal swab nucleic acid test more accurate than nasopharyngeal swab?

Nucleic acid detection:generally carried out by sampling pharyngeal swabs in the nasal cavity and oral cavity.

Now began to increase the anal swab test, we are also curious about this test method. So, is anal swab nucleic acid detection more accurate than nasopharyngeal swab? How to sample for anal swab test?

Is the nucleic acid detection of anal swab more accurate than that of nasopharyngeal swab?

In the screening of New Coronavirus pneumonia, we mainly used the oropharyngeal swab nucleic acid test. Because the reason is convenient and quick, suitable for large-scale screening. The accuracy of nasopharynx swab for new crown nucleic acid detection is higher than that of oropharyngeal swab, but the human body feels uncomfortable.

In last year’s test, researchers found that many infected people had negative pharyngeal nucleic acid.But their anal swabs were still positive for longer.

The combination of the two in nucleic acid detection can improve the detection rate and reduce the proportion of missed diagnosis.

However, anal swab sampling is inconvenient and not suitable for large-scale screening and testing in the community.

For some asymptomatic or mild infections, the condition recovers quickly after infection. And the pharyngeal nucleic acid may not be detected in 3 to 5 days.

The researchers found that the duration of nucleic acid positive in feces or anal swabs of some infected people was longer than that in the upper respiratory tract.

Therefore, increasing anal swab nucleic acid detection can improve the detection rate of infected patients and reduce missed diagnosis.

Although nucleic acid detection has the advantages of high sensitivity and short detection cycle, it also has some disadvantages.

Different from the direct isolation of live virus from human body, nucleic acid detection adopts an indirect method, which may cause false negative.

Anal swab & test tubes

Anal swab & test tubes

How to sample for anal swab test

1. Soak the swab in normal saline, insert it into the anus of 2-3 cm, wipe it from the folds around the anus, or rotate it gently in the anus. And then insert it into the test tube containing normal saline.

2. For fecal swab culture, all the above operations require the use of sterile equipment and the swab into a sterile test tube.

anal swab

Disposable virus sampler

Disposable virus sampler

Disposable virus sampler

♦ Product Name:

① General name: disposable sampler

② Name: sample collection kit

③ Specification and model: cm-93050t, cm-93050rt, cm-93050rtz, cm-96000t, cm-96000ts, cm98000t, cm-98000tl, cm-761tz

♦ Package specification: 50 pcs / bag

♦ Intended use: disposable virus sampling tube_ Sampling swab_ Flocking swab_ Saliva collector_ Nucleic acid detection sampling kit_ Throat swab – cell medical (Dongguan) Co., Ltd.

Medical staff use DNA samplers and disposable samplers for sample collection, transportation and storage.

♦ Structure composition: swabs and test tubes. Non sterile.

Disposable virus sampler_cm-93050t

Disposable virus sampler_cm-93050t

♦ Storage conditions and validity period:

① Products shall be stored in a clean, hygienic, dry and ventilated environment;

② . the temperature is 5 ℃ ~ 35 ℃;

③ . relative humidity < 85% RH;

④ Product validity: two years

♦ usage method:

① Before sampling, relevant information shall be marked on the label of the sampling tube.

② . use the corresponding sampling flocked swab for sampling.

③ After sampling, quickly put the cotton swab into the sampling tube and tighten the tube cover.

④ . see the following for specific sampling methods:

a) Nasal swab: gently insert the sampling head into the nasopalatine part of the nasal canal, stay for a moment, then slowly rotate and exit, put the collected specimen into the sampling tube, and tighten the cover of the sampling tube.

b) Pharyngeal swab: wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with the sampling head, put the collected samples into the sampling tube, and tighten the cover of the sampling tube.

c) Sampling of mycoplasma, chlamydia and Ureaplasma samples:

Male: insert the sampling head into the urethra and rotate about 2cm. Stay for a moment and then exit. Put the collected specimen into the sampling tube and tighten the cover of the sampling tube.

Female: wipe the mucus from the cervical mouth, insert the sampling head into the cervical tube for 1-2cm for sampling, put the collected sample into the sampling tube, and tighten the cover of the sampling tube.

♦ matters needing attention:

① . After collecting the sample, the medical staff shall completely insert the disposable sampling cotton swab into the test tube to retain the sample to the greatest extent.

② . the collected samples must be fresh and submitted for inspection in time.

③ .forbidden to use products with damaged packaging and expiration date to prevent pollution.

④ . forbidden to directly contact the collected patient, and forbidden to sample the patient after wetting the swab.

⑤ . When collecting samples, medical staff should strictly implement the sampling procedures to ensure that the sampling position is accurate and the sampling intensity is uniform and appropriate, otherwise the sample collection quality will be affected.

⑥ The product shall not be used after the expiration date or the product package is damaged.

Note: this disposable sampler : for in vitro diagnosis and not for human or animal internal and external use.

If swallowed, it may lead to serious events and cause certain irritation to eyes and skin. If it accidentally splashes into eyes, rinse with clean water.

♦ essential information

Name of manufacturer: cell medical (Dongguan) Co., Ltd

Domicile: No. 98, Wusha street, Chang’an Town, Dongguan City, Guangdong Province, China

E-mail: flockedswabs@gmail.com

Sample collection swab :How much do you know about nucleic acid testing

Sample collection swab :How much do you know about nucleic acid testing

Sample collection swab:How much do you know about nucleic acid testing

The spread of the covid-19  is continuing worldwide. Although the global scientific and medical circles are making every effort to fight the epidemic, it is difficult to predict how the epidemic will develop in the future, and its impact on social development has appeared in many aspects.

Currently, COVID-19 cases are over 220 million.

Currently, COVID-19 cases are over 220 million.

In order to fully control covid-19, the general screening of local areas shall be carried out if necessary.

After identifying the detection object, how to detect it?

1、 What specimen to choose?

According to the recommendations of the American infectious diseases association, the following specimens were used:

The sensitivity of simple oropharyngeal swab may not be enough, so it is relatively easy to miss diagnosis.

The sensitivity of alveolar lavage fluid is the highest, but it is obviously not suitable for universal screening.

Nasopharynx swabs and other operations should also be standardized.

Improper operation may make the quality of collected samples not high, thus increasing the risk of missed diagnosis.

2、 Select the time of detection

It is very important for COVID-19 to measure RNA and choose the right time. One analysis included 7 studies, including 2 unpublished reports, to evaluate the detection efficiency of RT-PCR at different time points after exposure to virus :

On the day of exposure to the virus, the possibility of detecting viral RNA is almost zero;

On the fifth day after exposure, that is, the first day after infection, the detection probability was 62%

About the 8th day after exposure, i.e. the 4th day after infection, the probability of detection was 80%

On the 21st day after exposure, i.e. the 17th day after infection, the detection probability was 34%

In conclusion, exposure to COVID-19 may require multiple tests to avoid missed diagnosis. However, it is not recommended to repeat the test within 24 hours of the previous test; The interval between two tests shall be at least 24 hours.

After 28 days of exposure to the virus, if the infection has not been diagnosed, it is not necessary to continue the detection; At this time, it is almost certain that it is not infected.

Nucleic acid detection throat swab  sampling process

The COVID-19 nucleic acid was novel coronavirus, which was collected from sputum or lower respiratory tract by real-time fluorescent RT-PCR. If positive, the new coronavirus could be diagnosed. This is the basis for the diagnosis of new type of pneumonia. In addition to detecting the nucleic acid of the virus, it can also detect whether the virus in the specimen is highly homologous with New Coronavirus. If so, it can be diagnosed as New Coronavirus pneumonia. The virus is highly infectious and has the characteristics of human to human transmission, but the virus is sensitive to temperature and sunlight and ultraviolet rays.

Sample collection, nasopharyngeal swab sampling

Sample collection, nasopharyngeal swab sampling

Sample collection, Oropharyngeal swab sampling

Sample collection, Oropharyngeal swab sampling

The flocked swab is the best raw material for sampling swabs.

 

 

reference

1,Infectious Diseases Society of America Guidelines on the Diagnosis of COVID-19, updated December 23, 2020. https://www.idsociety.org/practice-guideline/covid-19-guideline-diagnostics/ (Accessed on January 14, 2021).

2,Kucirka LM, Lauer SA, Laeyendecker O, et al. Variation in False-Negative Rate of Reverse Transcriptase Polymerase Chain Reaction-Based SARS-CoV-2 Tests by Time Since Exposure. Ann Intern Med 2020; 173:262.

 

COVID-19 Testing: Method of collecting virus swab samples

COVID-19 Testing: Method of collecting virus swab samples

COVID-19 Testing: virus swab

According to New Coronavirus’s pneumonia diagnosis and treatment plan (trial version fourth), to diagnose suspected cases of New Coronavirus pneumonia, we need one of the following etiological evidence:

1. Respiratory tract specimens or blood samples were detected by real-time fluorescence RT-PCR for New Coronavirus nucleic acid positive.

2. Novel coronavirus is highly homologous to respiratory tract specimens or blood samples.

Therefore, novel coronavirus pneumonia is an indispensable part of the specimen collection. So, where to collect specimens? How to collect specimens? How can medical staff protect themselves during collection? What should I do after collection?

How to obtain a virus swab specimen?

We all know that the collection method of virus swab specimen is basically to use the swab to sample the infected place or part of the virus, and then put it into the virus sampling tube containing preservation solution without touching other places, so as to conduct virus detection later.

Specimen type:

1. Upper respiratory tract specimens: including pharyngeal swab, nasal swab, nasopharyngeal extract, etc.

2. Lower respiratory tract specimens: including deep expectoration fluid, respiratory tract extract, bronchial lavage fluid, alveolar lavage fluid and lung tissue biopsy specimens.

3. Blood samples: try to collect anticoagulants in the acute stage within 7 days after onset. The collection volume is 5 ml, preferably fasting blood.

4. Serum samples: try to collect double serum in acute stage and recovery stage.

5. Conjunctival specimens: conjunctival swab specimens shall be collected for cases with ocular infection symptoms.

6. Stool samples: Patients with diarrhea symptoms need to collect stool samples.

COVID-19 Testing: Common virus swab sampling methods

Methods of throat swab collection

Inform the patient of the purpose of examination, collection method and collection time. It is best to collect specimens before using antibiotics.

Objective: To study the clinical effect of the method

Secretions were collected from pharynx or tonsil for bacterial culture or virus isolation to assist in clinical diagnosis and treatment.

Prepare sampling supplies:

Disposable sampling swab, virus sampling tube, tongue depressor and biological bag

Operation method:

(1) Ask the patient to sit down, tilt his head back, open his mouth and make a “ah ~” sound

(2) The sampling personnel shall fix the patient’s tongue with a tongue depressor, and use a disposable pharyngeal swab to pass through the root of the tongue to the posterior pharyngeal wall, tonsil recess and lateral wall;

(3) Repeatedly wipe 3-5 times to collect mucous membrane cells or sputum

(4) . gently remove the swab to avoid touching the tongue, the suspension, the oral mucosa and saliva

(5) Put the swab into the virus sampling tube gently and vertically. The sampling personnel shall disconnect the tail of the flocking swab and leave the tail of the swab completely in the tube.Be careful that the swab does not touch the wall of the tube.

(6) . tighten the cap, mark it, and seal it in the biosafety bag.

(7) The sampling personnel shall send the samples to the laboratory for testing in time. If the sampling personnel cannot send the samples for inspection in time, they shall store them at low temperature

Methods: nasal swabs were collected

Nasopharyngeal swab_COVID-19 Testing: Method of collecting virus swab samples

Nasopharyngeal swab_COVID-19 Testing: Method of collecting virus swab samples

(1) Ask the patient to sit down with his head slightly back and remove the secretion from the front nostril first;

(2)measure the distance from nostril to ear base with swab and mark with finger

(3)gently insert the swab into the nostril until it is marked or stopped when there is obstruction. Let the swab stay in the nose for 15-30 seconds, and then rotate it gently three times.

(4) slowly pull out the nasal swab and quickly put it into the virus sampling tube. Then break the swab, leave its tail and place it completely in the tube.

(5) tighten the cap, mark it, and seal it in the biosafety bag.

(6) If we need to collect from both nostrils, we should use a swab.

(7) It should be sent to the laboratory for testing in time. If it can not be sent for inspection in time, it should be stored at low temperature.

Generally speaking, COVID-19 testing: the main methods of collecting virus swabs are nasopharyngeal sampling and oropharyngeal sampling.

 

Is COVID-19 pneumonia more infectious than SARS?

The effectiveness of infectious disease transmission depends on its basic regeneration index. Novel coronavirus pneumonia is more infectious than SARS at present, with a basic regeneration index of SARS higher than that of SARS.

The novel coronavirus pneumonia is a new disease, and the population is generally immune. Everyone is susceptible and susceptible to infection. The incubation period of COVID-19 also has the ability to transmit, and it has longer infection time. The novel coronavirus pneumonia novel coronavirus pneumonia is also typical of the clinical symptoms, and asymptomatic infections are also contagious, so the infection of new crown pneumonia is higher than SARS.

About us

Cell medical (Dongguan) Co., Ltd.  is a swabs manufacturer. We produce and sell virus sampling related products. Such as virus sampling flocked swabs, VTM kits and virus transport media, and have multiple automatic production lines.  We support customized production, OEM, etc., and develop products with customers.